Restriction map for
pF1KB4967
(Linear) (Six-base) MAPSORT of: KIBB4967.seq Check: 5009 from: 1 to: 7242
KIBB4967.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5014 5089 5775 6748 7242
Size: 5014 75 686 973 494
Fragments arranged by size:
5014 973 686 494 75
AccI GT'mk_AC
Cuts at: 0 3413 4178 5000 6768 7242
Size: 3413 765 822 1768 474
Fragments arranged by size:
3413 1768 822 765 474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 175 652 2182 3080 4493 6778 7242
Size: 17 158 477 1530 898 1413 2285 464
Fragments arranged by size:
2285 1530 1413 898 477 464 158 17
AfeI AGC'GCT
Cuts at: 0 5209 7242
Size: 5209 2033
AflII C'TTAA_G
Cuts at: 0 724 7242
Size: 724 6518
AflIII A'CryG_T
Cuts at: 0 1853 7242
Size: 1853 5389
AhdI GACnn_n'nnGTC
Cuts at: 0 5921 7242
Size: 5921 1321
AleI CACnn'nnGTG
Cuts at: 0 2881 4615 7242
Size: 2881 1734 2627
Fragments arranged by size:
2881 2627 1734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5372 6281 7242
Size: 5372 909 961
Fragments arranged by size:
5372 961 909
AlwNI CAG_nnn'CTG
Cuts at: 0 883 2584 2767 3171 3522 5223 6133 7242
Size: 883 1701 183 404 351 1701 910 1109
Fragments arranged by size:
1701 1701 1109 910 883 404 351 183
ApaI G_GGCC'C
Cuts at: 0 5762 7242
Size: 5762 1480
ApaLI G'TGCA_C
Cuts at: 0 3546 3836 7242
Size: 3546 290 3406
Fragments arranged by size:
3546 3406 290
ApoI r'AATT_y
Cuts at: 0 1611 1697 2487 2886 5837 7107 7206 7242
Size: 1611 86 790 399 2951 1270 99 36
Fragments arranged by size:
2951 1611 1270 790 399 99 86 36
AseI AT'TA_AT
Cuts at: 0 139 182 7242
Size: 139 43 7060
Fragments arranged by size:
7060 139 43
AvaI C'yCGr_G
Cuts at: 0 4711 7242
Size: 4711 2531
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3474 3507 5058 5091 7242
Size: 3474 33 1551 33 2151
Fragments arranged by size:
3474 2151 1551 33 33
BamHI G'GATC_C
Cuts at: 0 1785 3204 3717 7242
Size: 1785 1419 513 3525
Fragments arranged by size:
3525 1785 1419 513
BanI G'GyrC_C
Cuts at: 0 3895 5333 5406 6760 7242
Size: 3895 1438 73 1354 482
Fragments arranged by size:
3895 1438 1354 482 73
BanII G_rGCy'C
Cuts at: 0 679 2232 2553 3286 3341 4941 5179 5762
Size: 679 1553 321 733 55 1600 238 583
Cuts at: 5762 6487 6838 7242
Size: 725 351 404
Fragments arranged by size:
1600 1553 733 725 679 583 404 351
321 238 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1570 3014 3086 4773 7242
Size: 1570 1444 72 1687 2469
Fragments arranged by size:
2469 1687 1570 1444 72
BbvCI CC'TCA_GC
Cuts at: 0 3242 5624 6002 6998 7242
Size: 3242 2382 378 996 244
Fragments arranged by size:
3242 2382 996 378 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2722 2756 5946 5980 7242
Size: 2722 34 3190 34 1262
Fragments arranged by size:
3190 2722 1262 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3475 5212 6226 7242
Size: 3475 1737 1014 1016
Fragments arranged by size:
3475 1737 1016 1014
BfrBI ATG'CAT
Cuts at: 0 3762 4128 7242
Size: 3762 366 3114
Fragments arranged by size:
3762 3114 366
BglI GCCn_nnn'nGGC
Cuts at: 0 70 319 1151 2613 6324 7242
Size: 70 249 832 1462 3711 918
Fragments arranged by size:
3711 1462 918 832 249 70
BglII A'GATC_T
Cuts at: 0 3767 7242
Size: 3767 3475
BlpI GC'TnA_GC
Cuts at: 0 2552 5741 6494 7242
Size: 2552 3189 753 748
Fragments arranged by size:
3189 2552 753 748
Bme1580I G_kGCm'C
Cuts at: 0 418 1652 3550 3840 5338 5762 7242
Size: 418 1234 1898 290 1498 424 1480
Fragments arranged by size:
1898 1498 1480 1234 424 418 290
BmrI ACTGGGnnnn_n'
Cuts at: 0 3099 3457 4935 5921 6861 7242
Size: 3099 358 1478 986 940 381
Fragments arranged by size:
3099 1478 986 940 381 358
BmtI G_CTAG'C
Cuts at: 0 7133 7242
Size: 7133 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4161 7242
Size: 4161 3081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 935 995 1313 2246 2351 2411 3104
Size: 799 136 60 318 933 105 60 693
Cuts at: 3104 4936 6003 6445 6957 7242
Size: 1832 1067 442 512 285
Fragments arranged by size:
1832 1067 933 799 693 512 442 318
285 136 105 60 60
Bpu10I CC'TnA_GC
Cuts at: 0 1357 3242 5624 6002 6998 7242
Size: 1357 1885 2382 378 996 244
Fragments arranged by size:
2382 1885 1357 996 378 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2360 4245 5654 6567 7242
Size: 2360 1885 1409 913 675
Fragments arranged by size:
2360 1885 1409 913 675
BsaI GGTCTCn'nnnn_
Cuts at: 0 817 2438 6182 7242
Size: 817 1621 3744 1060
Fragments arranged by size:
3744 1621 1060 817
BsaAI yAC'GTr
Cuts at: 0 5832 7242
Size: 5832 1410
BsaBI GATnn'nnATC
Cuts at: 0 182 4844 7242
Size: 182 4662 2398
Fragments arranged by size:
4662 2398 182
BsaHI Gr'CG_yC
Cuts at: 0 73 5602 7242
Size: 73 5529 1640
Fragments arranged by size:
5529 1640 73
BsaWI w'CCGG_w
Cuts at: 0 3395 7242
Size: 3395 3847
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1286 1316 5522 5552 7242
Size: 1286 30 4206 30 1690
Fragments arranged by size:
4206 1690 1286 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2183 3088 4185 5720 7242
Size: 2183 905 1097 1535 1522
Fragments arranged by size:
2183 1535 1522 1097 905
BseYI C'CCAG_C
Cuts at: 0 716 1381 2162 2459 2729 2822 3659 5606
Size: 716 665 781 297 270 93 837 1947
Cuts at: 5606 6008 6056 6181 6233 6437 6839 6929 6959
Size: 402 48 125 52 204 402 90 30
Cuts at: 6959 7242
Size: 283
Fragments arranged by size:
1947 837 781 716 665 402 402 297
283 270 204 125 93 90 52 48
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2693 2710 4957 5314 7242
Size: 2693 17 2247 357 1928
Fragments arranged by size:
2693 2247 1928 357 17
BsiHKAI G_wGCw'C
Cuts at: 0 122 981 1299 2232 2758 3550 3840 7242
Size: 122 859 318 933 526 792 290 3402
Fragments arranged by size:
3402 933 859 792 526 318 290 122
BsmI GAATG_Cn'
Cuts at: 0 513 899 1599 5020 5253 6247 7242
Size: 513 386 700 3421 233 994 995
Fragments arranged by size:
3421 995 994 700 513 386 233
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5803 6543 7242
Size: 5803 740 699
Fragments arranged by size:
5803 740 699
Bsp1286I G_dGCh'C
Cuts at: 0 122 418 679 981 1299 1652 2232 2553
Size: 122 296 261 302 318 353 580 321
Cuts at: 2553 2758 3286 3341 3550 3840 4941 5179 5338
Size: 205 528 55 209 290 1101 238 159
Cuts at: 5338 5762 6487 6838 7242
Size: 424 725 351 404
Fragments arranged by size:
1101 725 580 528 424 404 353 351
321 318 302 296 290 261 238 209
205 159 122 55
BspHI T'CATG_A
Cuts at: 0 572 4242 7242
Size: 572 3670 3000
Fragments arranged by size:
3670 3000 572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 915 5014 5089 5775 6748 7242
Size: 915 4099 75 686 973 494
Fragments arranged by size:
4099 973 915 686 494 75
BsrBI CCG'CTC
Cuts at: 0 1879 7242
Size: 1879 5363
BsrDI GCAATG_nn'
Cuts at: 0 549 2897 4344 4608 4799 5016 5354 5792
Size: 549 2348 1447 264 191 217 338 438
Cuts at: 5792 7242
Size: 1450
Fragments arranged by size:
2348 1450 1447 549 438 338 264 217
191
BsrGI T'GTAC_A
Cuts at: 0 1216 3739 7242
Size: 1216 2523 3503
Fragments arranged by size:
3503 2523 1216
BssSI C'ACGA_G
Cuts at: 0 3557 7242
Size: 3557 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1160 1177 1968 2716 5015 5451 6207 6564
Size: 1160 17 791 748 2299 436 756 357
Cuts at: 6564 6678 6690 6699 6849 7242
Size: 114 12 9 150 393
Fragments arranged by size:
2299 1160 791 756 748 436 393 357
150 114 17 12 9
BstBI TT'CG_AA
Cuts at: 0 1737 7242
Size: 1737 5505
BstEII G'GTnAC_C
Cuts at: 0 5712 7242
Size: 5712 1530
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4536 6649 7074 7176 7242
Size: 4536 2113 425 102 66
Fragments arranged by size:
4536 2113 425 102 66
BstYI r'GATC_y
Cuts at: 0 1731 1785 1916 3204 3291 3620 3717 3767
Size: 1731 54 131 1288 87 329 97 50
Cuts at: 3767 6893 7242
Size: 3126 349
Fragments arranged by size:
3126 1731 1288 349 329 131 97 87
54 50
BstZ17I GTA'TAC
Cuts at: 0 3414 4179 7242
Size: 3414 765 3063
Fragments arranged by size:
3414 3063 765
BtgI C'CryG_G
Cuts at: 0 485 2082 2205 3287 3995 4760 5882 5891
Size: 485 1597 123 1082 708 765 1122 9
Cuts at: 5891 6470 6946 7073 7155 7169 7242
Size: 579 476 127 82 14 73
Fragments arranged by size:
1597 1122 1082 765 708 579 485 476
127 123 82 73 14 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 98 7242
Size: 98 7144
BtsI GCAGTG_nn'
Cuts at: 0 1093 1140 2094 5260 5382 5780 7242
Size: 1093 47 954 3166 122 398 1462
Fragments arranged by size:
3166 1462 1093 954 398 122 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4010 4045 5639 5674 7242
Size: 4010 35 1594 35 1568
Fragments arranged by size:
4010 1594 1568 35 35
DraI TTT'AAA
Cuts at: 0 1799 3827 4461 7242
Size: 1799 2028 634 2781
Fragments arranged by size:
2781 2028 1799 634
DraIII CAC_nnn'GTG
Cuts at: 0 1177 4321 4955 5028 7242
Size: 1177 3144 634 73 2214
Fragments arranged by size:
3144 2214 1177 634 73
DrdI GACnn_nn'nnGTC
Cuts at: 0 1229 4996 6174 7242
Size: 1229 3767 1178 1068
Fragments arranged by size:
3767 1229 1178 1068
EaeI y'GGCC_r
Cuts at: 0 2 311 2145 5495 5577 6614 6949 7242
Size: 2 309 1834 3350 82 1037 335 293
Fragments arranged by size:
3350 1834 1037 335 309 293 82 2
EarI CTCTTCn'nnn_
Cuts at: 0 671 2201 2483 3032 3274 4568 4929 7242
Size: 671 1530 282 549 242 1294 361 2313
Fragments arranged by size:
2313 1530 1294 671 549 361 282 242
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 4783 7242
Size: 66 4717 2459
Fragments arranged by size:
4717 2459 66
EcoICRI GAG'CTC
Cuts at: 0 2230 7242
Size: 2230 5012
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 175 652 799 935 995 1313 2182
Size: 17 158 477 147 136 60 318 869
Cuts at: 2182 2246 2351 2411 3080 3104 4493 4936 6003
Size: 64 105 60 669 24 1389 443 1067
Cuts at: 6003 6445 6778 6957 7242
Size: 442 333 179 285
Fragments arranged by size:
1389 1067 869 669 477 443 442 333
318 285 179 158 147 136 105 64
60 60 24 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 341 887 3950 7242
Size: 341 546 3063 3292
Fragments arranged by size:
3292 3063 546 341
EcoO109I rG'GnC_Cy
Cuts at: 0 436 712 1660 2014 5535 5758 5759 5923
Size: 436 276 948 354 3521 223 1 164
Cuts at: 5923 6604 6809 7242
Size: 681 205 433
Fragments arranged by size:
3521 948 681 436 433 354 276 223
205 164 1
EcoRI G'AATT_C
Cuts at: 0 1697 2487 2886 7107 7206 7242
Size: 1697 790 399 4221 99 36
Fragments arranged by size:
4221 1697 790 399 99 36
HaeII r_GCGC'y
Cuts at: 0 1059 5211 5820 7169 7242
Size: 1059 4152 609 1349 73
Fragments arranged by size:
4152 1349 1059 609 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 450 482 648 680 1369 1401
Size: 85 32 333 32 166 32 689 32
Cuts at: 1401 1518 1550 2094 2126 3196 3228 4860 4892
Size: 117 32 544 32 1070 32 1632 32
Cuts at: 4892 5907 5939 6669 6701 6981 7013 7242
Size: 1015 32 730 32 280 32 229
Fragments arranged by size:
1632 1070 1015 730 689 544 333 280
229 166 117 85 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 449 910 1339 3229 4107 6067 7242
Size: 449 461 429 1890 878 1960 1175
Fragments arranged by size:
1960 1890 1175 878 461 449 429
MfeI C'AATT_G
Cuts at: 0 584 5253 7242
Size: 584 4669 1989
Fragments arranged by size:
4669 1989 584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1284 2300 3482 5224 5587 5746 5880 7242
Size: 1284 1016 1182 1742 363 159 134 1362
Fragments arranged by size:
1742 1362 1284 1182 1016 363 159 134
MscI TGG'CCA
Cuts at: 0 313 2147 5497 5579 6616 7242
Size: 313 1834 3350 82 1037 626
Fragments arranged by size:
3350 1834 1037 626 313 82
MslI CAynn'nnrTG
Cuts at: 0 409 490 502 2881 3599 4615 4957 4963
Size: 409 81 12 2379 718 1016 342 6
Cuts at: 4963 5896 7242
Size: 933 1346
Fragments arranged by size:
2379 1346 1016 933 718 409 342 81
12 6
MspA1I CmG'CkG
Cuts at: 0 240 988 2357 2790 2830 5451 5508 5806
Size: 240 748 1369 433 40 2621 57 298
Cuts at: 5806 6237 6261 6675 7242
Size: 431 24 414 567
Fragments arranged by size:
2621 1369 748 567 433 431 414 298
240 57 40 24
NcoI C'CATG_G
Cuts at: 0 485 2205 3287 4760 5882 5891 6470 7073
Size: 485 1720 1082 1473 1122 9 579 603
Cuts at: 7073 7155 7242
Size: 82 87
Fragments arranged by size:
1720 1473 1122 1082 603 579 485 87
82 9
NdeI CA'TA_TG
Cuts at: 0 1830 6848 7242
Size: 1830 5018 394
Fragments arranged by size:
5018 1830 394
NheI G'CTAG_C
Cuts at: 0 7129 7242
Size: 7129 113
NsiI A_TGCA'T
Cuts at: 0 3764 4130 7242
Size: 3764 366 3112
Fragments arranged by size:
3764 3112 366
NspI r_CATG'y
Cuts at: 0 1042 1509 1857 1971 4128 4132 4313 4962
Size: 1042 467 348 114 2157 4 181 649
Cuts at: 4962 5505 5649 6318 6693 7242
Size: 543 144 669 375 549
Fragments arranged by size:
2157 1042 669 649 549 543 467 375
348 181 144 114 4
PasI CC'CwG_GG
Cuts at: 0 1656 5691 6301 6321 7242
Size: 1656 4035 610 20 921
Fragments arranged by size:
4035 1656 921 610 20
PciI A'CATG_T
Cuts at: 0 1853 7242
Size: 1853 5389
PflMI CCAn_nnn'nTGG
Cuts at: 0 966 2128 5112 5223 6240 6744 7045 7242
Size: 966 1162 2984 111 1017 504 301 197
Fragments arranged by size:
2984 1162 1017 966 504 301 197 111
PfoI T'CCnGG_A
Cuts at: 0 336 522 780 3120 7044 7242
Size: 336 186 258 2340 3924 198
Fragments arranged by size:
3924 2340 336 258 198 186
PmlI CAC'GTG
Cuts at: 0 5832 7242
Size: 5832 1410
PpuMI rG'GwC_Cy
Cuts at: 0 436 1660 5923 6809 7242
Size: 436 1224 4263 886 433
Fragments arranged by size:
4263 1224 886 436 433
PshAI GACnn'nnGTC
Cuts at: 0 4234 7242
Size: 4234 3008
PspOMI G'GGCC_C
Cuts at: 0 5758 7242
Size: 5758 1484
PstI C_TGCA'G
Cuts at: 0 540 1986 4236 4391 5166 5456 5772 6197
Size: 540 1446 2250 155 775 290 316 425
Cuts at: 6197 7242
Size: 1045
Fragments arranged by size:
2250 1446 1045 775 540 425 316 290
155
PvuII CAG'CTG
Cuts at: 0 240 988 2830 5451 6237 7242
Size: 240 748 1842 2621 786 1005
Fragments arranged by size:
2621 1842 1005 786 748 240
SacI G_AGCT'C
Cuts at: 0 2232 7242
Size: 2232 5010
SanDI GG'GwC_CC
Cuts at: 0 6809 7242
Size: 6809 433
SapI GCTCTTCn'nnn_
Cuts at: 0 2483 3274 4929 7242
Size: 2483 791 1655 2313
Fragments arranged by size:
2483 2313 1655 791
ScaI AGT'ACT
Cuts at: 0 3093 7242
Size: 3093 4149
SfcI C'TryA_G
Cuts at: 0 149 536 1954 1982 4232 4387 5162 5452
Size: 149 387 1418 28 2250 155 775 290
Cuts at: 5452 5464 5768 6016 6193 6337 6769 7242
Size: 12 304 248 177 144 432 473
Fragments arranged by size:
2250 1418 775 473 432 387 304 290
248 177 155 149 144 28 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 319 7242
Size: 319 6923
SmaI CCC'GGG
Cuts at: 0 4713 7242
Size: 4713 2529
SmlI C'TyrA_G
Cuts at: 0 724 2375 4224 5669 6546 7242
Size: 724 1651 1849 1445 877 696
Fragments arranged by size:
1849 1651 1445 877 724 696
SpeI A'CTAG_T
Cuts at: 0 418 2869 7242
Size: 418 2451 4373
Fragments arranged by size:
4373 2451 418
SphI G_CATG'C
Cuts at: 0 1042 1509 4128 4962 5505 5649 6318 7242
Size: 1042 467 2619 834 543 144 669 924
Fragments arranged by size:
2619 1042 924 834 669 543 467 144
StuI AGG'CCT
Cuts at: 0 941 5126 6742 6775 7242
Size: 941 4185 1616 33 467
Fragments arranged by size:
4185 1616 941 467 33
StyI C'CwwG_G
Cuts at: 0 485 807 960 1500 2205 2644 3287 3477
Size: 485 322 153 540 705 439 643 190
Cuts at: 3477 3624 4622 4760 5127 5303 5882 5891 5988
Size: 147 998 138 367 176 579 9 97
Cuts at: 5988 6031 6470 6642 6743 6985 7073 7155 7242
Size: 43 439 172 101 242 88 82 87
Fragments arranged by size:
998 705 643 579 540 485 439 439
367 322 242 190 176 172 153 147
138 101 97 88 87 82 43 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4176 5185 5970 6483 7242
Size: 4176 1009 785 513 759
Fragments arranged by size:
4176 1009 785 759 513
TatI w'GTAC_w
Cuts at: 0 834 1216 3091 3739 5047 7225 7242
Size: 834 382 1875 648 1308 2178 17
Fragments arranged by size:
2178 1875 1308 834 648 382 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3702 3734 6281 6313 7003 7035 7242
Size: 3702 32 2547 32 690 32 207
Fragments arranged by size:
3702 2547 690 207 32 32 32
XbaI T'CTAG_A
Cuts at: 0 3903 7242
Size: 3903 3339
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 2946 4959 5889 5934 6048 7242
Size: 408 2538 2013 930 45 114 1194
Fragments arranged by size:
2538 2013 1194 930 408 114 45
XmaI C'CCGG_G
Cuts at: 0 4711 7242
Size: 4711 2531
XmnI GAAnn'nnTTC
Cuts at: 0 2046 3215 3610 4055 7242
Size: 2046 1169 395 445 3187
Fragments arranged by size:
3187 2046 1169 445 395
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI ScaI SfcI SfiI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AgeI AloI AscI AsiSI AvrII
BbeI BclI BmgBI BsiEI BsiWI BspEI BsrFI BssHII
Bsu36I ClaI EagI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV
NotI NruI PacI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SalI SbfI SexAI SfoI SgfI
SgrAI SnaBI SrfI SspI SwaI Tth111I XhoI ZraI