Restriction map for
pF1KB4969
(Linear) (Six-base) MAPSORT of: KIBB4969.seq Check: 4631 from: 1 to: 6672
KIBB4969.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 5495 6672
Size: 5495 1177
AccI GT'mk_AC
Cuts at: 0 194 2544 3054 6672
Size: 194 2350 510 3618
Fragments arranged by size:
3618 2350 510 194
Acc65I G'GTAC_C
Cuts at: 0 1670 4709 6672
Size: 1670 3039 1963
Fragments arranged by size:
3039 1963 1670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 2165 2516 3079 3407 3849 3984 4778
Size: 296 1869 351 563 328 442 135 794
Cuts at: 4778 4970 5759 5974 6672
Size: 192 789 215 698
Fragments arranged by size:
1869 794 789 698 563 442 351 328
296 215 192 135
AflII C'TTAA_G
Cuts at: 0 1006 3190 6672
Size: 1006 2184 3482
Fragments arranged by size:
3482 2184 1006
AflIII A'CryG_T
Cuts at: 0 6321 6558 6672
Size: 6321 237 114
Fragments arranged by size:
6321 237 114
AleI CACnn'nnGTG
Cuts at: 0 6470 6672
Size: 6470 202
AlwNI CAG_nnn'CTG
Cuts at: 0 825 5717 6672
Size: 825 4892 955
Fragments arranged by size:
4892 955 825
ApaI G_GGCC'C
Cuts at: 0 566 904 6672
Size: 566 338 5768
Fragments arranged by size:
5768 566 338
ApoI r'AATT_y
Cuts at: 0 1859 1998 2032 2287 2566 3306 4196 4429
Size: 1859 139 34 255 279 740 890 233
Cuts at: 4429 4516 4783 4925 4983 5302 5866 6672
Size: 87 267 142 58 319 564 806
Fragments arranged by size:
1859 890 806 740 564 319 279 267
255 233 142 139 87 58 34
AseI AT'TA_AT
Cuts at: 0 5423 6672
Size: 5423 1249
AvaI C'yCGr_G
Cuts at: 0 549 783 4898 6137 6672
Size: 549 234 4115 1239 535
Fragments arranged by size:
4115 1239 549 535 234
BamHI G'GATC_C
Cuts at: 0 2511 5885 5924 6672
Size: 2511 3374 39 748
Fragments arranged by size:
3374 2511 748 39
BanI G'GyrC_C
Cuts at: 0 43 1670 4709 5938 6672
Size: 43 1627 3039 1229 734
Fragments arranged by size:
3039 1627 1229 734 43
BanII G_rGCy'C
Cuts at: 0 146 566 784 904 1275 3458 5801 6672
Size: 146 420 218 120 371 2183 2343 871
Fragments arranged by size:
2343 2183 871 420 371 218 146 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 619 2075 2504 2897 3192 4817 6672
Size: 619 1456 429 393 295 1625 1855
Fragments arranged by size:
1855 1625 1456 619 429 393 295
BbvCI CC'TCA_GC
Cuts at: 0 2023 3568 3622 3703 3730 3757 3838 3973
Size: 2023 1545 54 81 27 27 81 135
Cuts at: 3973 4108 4297 6672
Size: 135 189 2375
Fragments arranged by size:
2375 2023 1545 189 135 135 81 81
54 27 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2271 2305 6672
Size: 2271 34 4367
Fragments arranged by size:
4367 2271 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2062 2531 6672
Size: 2062 469 4141
Fragments arranged by size:
4141 2062 469
BclI T'GATC_A
Cuts at: 0 1412 2915 3405 4380 6672
Size: 1412 1503 490 975 2292
Fragments arranged by size:
2292 1503 1412 975 490
BfrBI ATG'CAT
Cuts at: 0 2127 6672
Size: 2127 4545
BglI GCCn_nnn'nGGC
Cuts at: 0 5176 6672
Size: 5176 1496
BglII A'GATC_T
Cuts at: 0 4185 4254 6014 6672
Size: 4185 69 1760 658
Fragments arranged by size:
4185 1760 658 69
BlpI GC'TnA_GC
Cuts at: 0 2588 6672
Size: 2588 4084
Bme1580I G_kGCm'C
Cuts at: 0 46 566 747 904 1886 3365 4634 6303
Size: 46 520 181 157 982 1479 1269 1669
Cuts at: 6303 6672
Size: 369
Fragments arranged by size:
1669 1479 1269 982 520 369 181 157
46
BmgBI CAC'GTC
Cuts at: 0 1524 6322 6672
Size: 1524 4798 350
Fragments arranged by size:
4798 1524 350
BmrI ACTGGGnnnn_n'
Cuts at: 0 1488 1889 3425 5802 6294 6672
Size: 1488 401 1536 2377 492 378
Fragments arranged by size:
2377 1536 1488 492 401 378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1306 6672
Size: 1306 5366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 690 847 2078 2293 2582 3326 3434
Size: 413 277 157 1231 215 289 744 108
Cuts at: 3434 3545 3572 3599 3626 3680 3707 3761 3788
Size: 111 27 27 27 54 27 54 27
Cuts at: 3788 3815 3869 3896 3950 4004 4058 4112 4220
Size: 27 54 27 54 54 54 54 108
Cuts at: 4220 4301 4355 4665 6672
Size: 81 54 310 2007
Fragments arranged by size:
2007 1231 744 413 310 289 277 215
157 111 108 108 81 54 54 54
54 54 54 54 54 27 27 27
27 27 27 27
Bpu10I CC'TnA_GC
Cuts at: 0 91 113 140 2023 2680 3568 3622 3703
Size: 91 22 27 1883 657 888 54 81
Cuts at: 3703 3730 3757 3838 3973 4108 4297 6672
Size: 27 27 81 135 135 189 2375
Fragments arranged by size:
2375 1883 888 657 189 135 135 91
81 81 54 27 27 27 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 326 348 1696 3490 6367 6672
Size: 138 188 22 1348 1794 2877 305
Fragments arranged by size:
2877 1794 1348 305 188 138 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 852 1133 6672
Size: 852 281 5539
Fragments arranged by size:
5539 852 281
BsaAI yAC'GTr
Cuts at: 0 105 2938 5124 5520 6672
Size: 105 2833 2186 396 1152
Fragments arranged by size:
2833 2186 1152 396 105
BsaWI w'CCGG_w
Cuts at: 0 2704 6672
Size: 2704 3968
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 447 477 1934 1964 2623 2653 2682 2712
Size: 447 30 1457 30 659 30 29 30
Cuts at: 2712 2733 2763 3570 3600 3624 3654 3705 3735
Size: 21 30 807 30 24 30 51 30
Cuts at: 3735 3894 3924 4218 4248 4299 4329 4660 4690
Size: 159 30 294 30 51 30 331 30
Cuts at: 4690 6672
Size: 1982
Fragments arranged by size:
1982 1457 807 659 447 331 294 159
51 51 30 30 30 30 30 30
30 30 30 30 30 30 29 24
21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 4358 4668 6672
Size: 4358 310 2004
Fragments arranged by size:
4358 2004 310
BseYI C'CCAG_C
Cuts at: 0 50 59 771 952 3669 3939 4047 4081
Size: 50 9 712 181 2717 270 108 34
Cuts at: 4081 6672
Size: 2591
Fragments arranged by size:
2717 2591 712 270 181 108 50 34
9
BsiHKAI G_wGCw'C
Cuts at: 0 784 1762 3458 6672
Size: 784 978 1696 3214
Fragments arranged by size:
3214 1696 978 784
BsmI GAATG_Cn'
Cuts at: 0 2478 2934 3195 6315 6333 6672
Size: 2478 456 261 3120 18 339
Fragments arranged by size:
3120 2478 456 339 261 18
Bsp1286I G_dGCh'C
Cuts at: 0 46 146 566 747 784 904 1275 1762
Size: 46 100 420 181 37 120 371 487
Cuts at: 1762 1886 3365 3458 4634 5801 6303 6672
Size: 124 1479 93 1176 1167 502 369
Fragments arranged by size:
1479 1176 1167 502 487 420 371 369
181 124 120 100 93 46 37
BspEI T'CCGG_A
Cuts at: 0 2704 6672
Size: 2704 3968
BspHI T'CATG_A
Cuts at: 0 1532 1754 6672
Size: 1532 222 4918
Fragments arranged by size:
4918 1532 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5495 6672
Size: 5495 1177
BsrDI GCAATG_nn'
Cuts at: 0 1058 3253 4414 4697 4748 6672
Size: 1058 2195 1161 283 51 1924
Fragments arranged by size:
2195 1924 1161 1058 283 51
BsrGI T'GTAC_A
Cuts at: 0 692 1184 4788 6672
Size: 692 492 3604 1884
Fragments arranged by size:
3604 1884 692 492
BssSI C'ACGA_G
Cuts at: 0 1019 6672
Size: 1019 5653
BstAPI GCAn_nnn'nTGC
Cuts at: 0 633 5869 6672
Size: 633 5236 803
Fragments arranged by size:
5236 803 633
BstBI TT'CG_AA
Cuts at: 0 4860 6672
Size: 4860 1812
BstEII G'GTnAC_C
Cuts at: 0 3576 6672
Size: 3576 3096
BstXI CCAn_nnnn'nTGG
Cuts at: 0 23 1879 2692 2743 6207 6672
Size: 23 1856 813 51 3464 465
Fragments arranged by size:
3464 1856 813 465 51 23
BstYI r'GATC_y
Cuts at: 0 1585 2511 3439 4185 4254 4347 4686 5827
Size: 1585 926 928 746 69 93 339 1141
Cuts at: 5827 5885 5924 6014 6125 6672
Size: 58 39 90 111 547
Fragments arranged by size:
1585 1141 928 926 746 547 339 111
93 90 69 58 39
Bsu36I CC'TnA_GG
Cuts at: 0 1392 6672
Size: 1392 5280
BtgI C'CryG_G
Cuts at: 0 5016 6515 6672
Size: 5016 1499 157
Fragments arranged by size:
5016 1499 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1527 6672
Size: 1527 5145
BtsI GCAGTG_nn'
Cuts at: 0 4605 6367 6672
Size: 4605 1762 305
Fragments arranged by size:
4605 1762 305
DraI TTT'AAA
Cuts at: 0 306 4980 5607 6672
Size: 306 4674 627 1065
Fragments arranged by size:
4674 1065 627 306
DraIII CAC_nnn'GTG
Cuts at: 0 2557 6672
Size: 2557 4115
EaeI y'GGCC_r
Cuts at: 0 816 1313 2988 5535 6672
Size: 816 497 1675 2547 1137
Fragments arranged by size:
2547 1675 1137 816 497
EarI CTCTTCn'nnn_
Cuts at: 0 1036 1386 1933 2147 2240 3236 3450 4580
Size: 1036 350 547 214 93 996 214 1130
Cuts at: 4580 4907 5332 6672
Size: 327 425 1340
Fragments arranged by size:
1340 1130 1036 996 547 425 350 327
214 214 93
EcoICRI GAG'CTC
Cuts at: 0 782 3456 6672
Size: 782 2674 3216
Fragments arranged by size:
3216 2674 782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 413 690 847 2078 2165 2293 2516
Size: 296 117 277 157 1231 87 128 223
Cuts at: 2516 2582 3079 3326 3407 3434 3545 3572 3599
Size: 66 497 247 81 27 111 27 27
Cuts at: 3599 3626 3680 3707 3761 3788 3815 3849 3869
Size: 27 54 27 54 27 27 34 20
Cuts at: 3869 3896 3950 3984 4004 4058 4112 4220 4301
Size: 27 54 34 20 54 54 108 81
Cuts at: 4301 4355 4665 4778 4970 5759 5974 6672
Size: 54 310 113 192 789 215 698
Fragments arranged by size:
1231 789 698 497 310 296 277 247
223 215 192 157 128 117 113 111
108 87 81 81 66 54 54 54
54 54 54 34 34 27 27 27
27 27 27 27 27 20 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 595 1395 3571 4300 6672
Size: 595 800 2176 729 2372
Fragments arranged by size:
2372 2176 800 729 595
EcoO109I rG'GnC_Cy
Cuts at: 0 27 563 587 822 1258 1847 2599 2714
Size: 27 536 24 235 436 589 752 115
Cuts at: 2714 2724 2765 2775 3137 5236 6672
Size: 10 41 10 362 2099 1436
Fragments arranged by size:
2099 1436 752 589 536 436 362 235
115 41 27 24 10 10
EcoRI G'AATT_C
Cuts at: 0 1998 2287 2566 3306 4925 6672
Size: 1998 289 279 740 1619 1747
Fragments arranged by size:
1998 1747 1619 740 289 279
EcoRV GAT'ATC
Cuts at: 0 4361 6672
Size: 4361 2311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 321 353 532 564 1132 1164 1242 1262
Size: 321 32 179 32 568 32 78 20
Cuts at: 1262 1274 1294 1531 1563 2056 2088 3570 3602
Size: 12 20 237 32 493 32 1482 32
Cuts at: 3602 3624 3656 3894 3926 4218 4250 4299 4331
Size: 22 32 238 32 292 32 49 32
Cuts at: 4331 4350 4382 4458 4490 5206 5238 5291 5323
Size: 19 32 76 32 716 32 53 32
Cuts at: 5323 6672
Size: 1349
Fragments arranged by size:
1482 1349 716 568 493 321 292 238
237 179 78 76 53 49 32 32
32 32 32 32 32 32 32 32
32 32 32 32 22 20 20 19
12
HincII GTy'rAC
Cuts at: 0 1377 1988 2904 6672
Size: 1377 611 916 3768
Fragments arranged by size:
3768 1377 916 611
HpaI GTT'AAC
Cuts at: 0 1988 2904 6672
Size: 1988 916 3768
Fragments arranged by size:
3768 1988 916
KpnI G_GTAC'C
Cuts at: 0 1674 4713 6672
Size: 1674 3039 1959
Fragments arranged by size:
3039 1959 1674
MfeI C'AATT_G
Cuts at: 0 2907 6672
Size: 2907 3765
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 261 1953 5643 6020 6157 6672
Size: 106 155 1692 3690 377 137 515
Fragments arranged by size:
3690 1692 515 377 155 137 106
MscI TGG'CCA
Cuts at: 0 1315 2990 6672
Size: 1315 1675 3682
Fragments arranged by size:
3682 1675 1315
MslI CAynn'nnrTG
Cuts at: 0 5015 5287 5518 6470 6672
Size: 5015 272 231 952 202
Fragments arranged by size:
5015 952 272 231 202
MspA1I CmG'CkG
Cuts at: 0 50 761 2416 2899 5197 6672
Size: 50 711 1655 483 2298 1475
Fragments arranged by size:
2298 1655 1475 711 483 50
NcoI C'CATG_G
Cuts at: 0 5016 6515 6672
Size: 5016 1499 157
Fragments arranged by size:
5016 1499 157
NdeI CA'TA_TG
Cuts at: 0 2076 3899 6672
Size: 2076 1823 2773
Fragments arranged by size:
2773 2076 1823
NsiI A_TGCA'T
Cuts at: 0 2129 6672
Size: 2129 4543
NspI r_CATG'y
Cuts at: 0 2587 5014 5134 5286 5901 6562 6672
Size: 2587 2427 120 152 615 661 110
Fragments arranged by size:
2587 2427 661 615 152 120 110
PasI CC'CwG_GG
Cuts at: 0 2717 6207 6315 6672
Size: 2717 3490 108 357
Fragments arranged by size:
3490 2717 357 108
PciI A'CATG_T
Cuts at: 0 6558 6672
Size: 6558 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 4243 4270 4459 6672
Size: 825 3418 27 189 2213
Fragments arranged by size:
3418 2213 825 189 27
PfoI T'CCnGG_A
Cuts at: 0 824 2641 4343 6672
Size: 824 1817 1702 2329
Fragments arranged by size:
2329 1817 1702 824
PmlI CAC'GTG
Cuts at: 0 2938 5520 6672
Size: 2938 2582 1152
Fragments arranged by size:
2938 2582 1152
PpuMI rG'GwC_Cy
Cuts at: 0 27 587 822 1258 1847 2599 2724 2775
Size: 27 560 235 436 589 752 125 51
Cuts at: 2775 3137 5236 6672
Size: 362 2099 1436
Fragments arranged by size:
2099 1436 752 589 560 436 362 235
125 51 27
PshAI GACnn'nnGTC
Cuts at: 0 3323 3431 3482 4460 6672
Size: 3323 108 51 978 2212
Fragments arranged by size:
3323 2212 978 108 51
PsiI TTA'TAA
Cuts at: 0 1172 1448 5834 6409 6672
Size: 1172 276 4386 575 263
Fragments arranged by size:
4386 1172 575 276 263
PspOMI G'GGCC_C
Cuts at: 0 562 900 6672
Size: 562 338 5772
Fragments arranged by size:
5772 562 338
PspXI vC'TCGA_Gb
Cuts at: 0 4898 6137 6672
Size: 4898 1239 535
Fragments arranged by size:
4898 1239 535
PstI C_TGCA'G
Cuts at: 0 1068 2196 6672
Size: 1068 1128 4476
Fragments arranged by size:
4476 1128 1068
PvuII CAG'CTG
Cuts at: 0 50 761 2416 2899 5197 6672
Size: 50 711 1655 483 2298 1475
Fragments arranged by size:
2298 1655 1475 711 483 50
SacI G_AGCT'C
Cuts at: 0 784 3458 6672
Size: 784 2674 3214
Fragments arranged by size:
3214 2674 784
SanDI GG'GwC_CC
Cuts at: 0 587 1847 2599 6672
Size: 587 1260 752 4073
Fragments arranged by size:
4073 1260 752 587
SapI GCTCTTCn'nnn_
Cuts at: 0 4907 5332 6672
Size: 4907 425 1340
Fragments arranged by size:
4907 1340 425
SfcI C'TryA_G
Cuts at: 0 195 1064 1326 1515 1836 2102 2192 2504
Size: 195 869 262 189 321 266 90 312
Cuts at: 2504 2518 5436 5718 6468 6672
Size: 14 2918 282 750 204
Fragments arranged by size:
2918 869 750 321 312 282 266 262
204 195 189 90 14
SmlI C'TyrA_G
Cuts at: 0 153 327 341 1006 1675 3190 3469 4898
Size: 153 174 14 665 669 1515 279 1429
Cuts at: 4898 6137 6382 6672
Size: 1239 245 290
Fragments arranged by size:
1515 1429 1239 669 665 290 279 245
174 153 14
SnaBI TAC'GTA
Cuts at: 0 5124 6672
Size: 5124 1548
SphI G_CATG'C
Cuts at: 0 5134 6672
Size: 5134 1538
SspI AAT'ATT
Cuts at: 0 2346 6672
Size: 2346 4326
StyI C'CwwG_G
Cuts at: 0 69 2603 4242 4458 5016 5881 5934 6341
Size: 69 2534 1639 216 558 865 53 407
Cuts at: 6341 6515 6672
Size: 174 157
Fragments arranged by size:
2534 1639 865 558 407 216 174 157
69 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 3008 3494 3667 3802 3937 4045 5463
Size: 496 2512 486 173 135 135 108 1418
Cuts at: 5463 6672
Size: 1209
Fragments arranged by size:
2512 1418 1209 496 486 173 135 135
108
TatI w'GTAC_w
Cuts at: 0 692 1184 4788 6672
Size: 692 492 3604 1884
Fragments arranged by size:
3604 1884 692 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 1934 1966 2623 2655 2682 2714
Size: 447 32 1455 32 657 32 27 32
Cuts at: 2714 2733 2765 6672
Size: 19 32 3907
Fragments arranged by size:
3907 1455 657 447 32 32 32 32
32 27 19
Tth111I GACn'n_nGTC
Cuts at: 0 1819 6672
Size: 1819 4853
XbaI T'CTAG_A
Cuts at: 0 3858 3993 4101 6672
Size: 3858 135 108 2571
Fragments arranged by size:
3858 2571 135 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 911 6672
Size: 911 5761
XhoI C'TCGA_G
Cuts at: 0 4898 6137 6672
Size: 4898 1239 535
Fragments arranged by size:
4898 1239 535
XmnI GAAnn'nnTTC
Cuts at: 0 3650 3663 6571 6672
Size: 3650 13 2908 101
Fragments arranged by size:
3650 2908 101 13
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlwNI
ApaI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV Hin4I HincII HpaI KpnI MfeI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuII SacI SanDI SapI SfcI SmlI SnaBI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AlfI AloI ApaLI
AscI AsiSI AvrII BaeI BbeI BmtI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BstZ17I
ClaI CspCI DrdI EagI EciI FalI FseI FspI
FspAI HaeII HindIII KasI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PmeI PpiI PsrI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SrfI StuI SwaI XmaI
ZraI