Restriction map for
pF1KB4982
(Linear) (Six-base) MAPSORT of: KIBB4982.seq Check: 3175 from: 1 to: 2403
KIBB4982.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AclI AA'CG_TT
Cuts at: 0 587 2403
Size: 587 1816
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1022 2403
Size: 416 606 1381
Fragments arranged by size:
1381 606 416
AflII C'TTAA_G
Cuts at: 0 2020 2403
Size: 2020 383
AleI CACnn'nnGTG
Cuts at: 0 1406 1856 2403
Size: 1406 450 547
Fragments arranged by size:
1406 547 450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 2403
Size: 94 2309
AlwNI CAG_nnn'CTG
Cuts at: 0 742 2332 2403
Size: 742 1590 71
Fragments arranged by size:
1590 742 71
ApaI G_GGCC'C
Cuts at: 0 2264 2403
Size: 2264 139
ApoI r'AATT_y
Cuts at: 0 1417 1497 2403
Size: 1417 80 906
Fragments arranged by size:
1417 906 80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1926 1959 2403
Size: 1926 33 444
Fragments arranged by size:
1926 444 33
BanI G'GyrC_C
Cuts at: 0 520 2403
Size: 520 1883
BanII G_rGCy'C
Cuts at: 0 187 791 2264 2403
Size: 187 604 1473 139
Fragments arranged by size:
1473 604 187 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 523 1085 2351 2403
Size: 523 562 1266 52
Fragments arranged by size:
1266 562 523 52
BbvCI CC'TCA_GC
Cuts at: 0 964 2403
Size: 964 1439
BfrBI ATG'CAT
Cuts at: 0 3 771 2403
Size: 3 768 1632
Fragments arranged by size:
1632 768 3
BglII A'GATC_T
Cuts at: 0 2319 2403
Size: 2319 84
Bme1580I G_kGCm'C
Cuts at: 0 525 852 1786 2264 2403
Size: 525 327 934 478 139
Fragments arranged by size:
934 525 478 327 139
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 533 2015 2403
Size: 145 388 1482 388
Fragments arranged by size:
1482 388 388 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2322 2403
Size: 2322 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 669 805 1307 2002 2253 2403
Size: 319 350 136 502 695 251 150
Fragments arranged by size:
695 502 350 319 251 150 136
Bpu10I CC'TnA_GC
Cuts at: 0 181 964 2403
Size: 181 783 1439
Fragments arranged by size:
1439 783 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1894 2050 2075 2154 2403
Size: 1894 156 25 79 249
Fragments arranged by size:
1894 249 156 79 25
BsaAI yAC'GTr
Cuts at: 0 2076 2403
Size: 2076 327
BsaBI GATnn'nnATC
Cuts at: 0 446 1631 2403
Size: 446 1185 772
Fragments arranged by size:
1185 772 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 261 291 913 943 1256 1286 2403
Size: 261 30 622 30 313 30 1117
Fragments arranged by size:
1117 622 313 261 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 978 2302 2403
Size: 646 332 1324 101
Fragments arranged by size:
1324 646 332 101
BseYI C'CCAG_C
Cuts at: 0 1888 2403
Size: 1888 515
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1521 2378 2403
Size: 1521 857 25
Fragments arranged by size:
1521 857 25
BsiHKAI G_wGCw'C
Cuts at: 0 187 791 886 1777 2081 2403
Size: 187 604 95 891 304 322
Fragments arranged by size:
891 604 322 304 187 95
BsmI GAATG_Cn'
Cuts at: 0 663 2403
Size: 663 1740
Bsp1286I G_dGCh'C
Cuts at: 0 187 525 791 852 886 1777 1786 2081
Size: 187 338 266 61 34 891 9 295
Cuts at: 2081 2264 2403
Size: 183 139
Fragments arranged by size:
891 338 295 266 187 183 139 61
34 9
BspHI T'CATG_A
Cuts at: 0 693 1515 2169 2403
Size: 693 822 654 234
Fragments arranged by size:
822 693 654 234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1904 2260 2403
Size: 1904 356 143
Fragments arranged by size:
1904 356 143
BsrDI GCAATG_nn'
Cuts at: 0 78 815 2403
Size: 78 737 1588
Fragments arranged by size:
1588 737 78
BsrGI T'GTAC_A
Cuts at: 0 2203 2403
Size: 2203 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1655 2076 2403
Size: 1655 421 327
Fragments arranged by size:
1655 421 327
BstEII G'GTnAC_C
Cuts at: 0 251 597 2403
Size: 251 346 1806
Fragments arranged by size:
1806 346 251
BstXI CCAn_nnnn'nTGG
Cuts at: 0 958 2403
Size: 958 1445
BstYI r'GATC_y
Cuts at: 0 441 2319 2403
Size: 441 1878 84
Fragments arranged by size:
1878 441 84
BtgI C'CryG_G
Cuts at: 0 1346 1737 2403
Size: 1346 391 666
Fragments arranged by size:
1346 666 391
BtsI GCAGTG_nn'
Cuts at: 0 1066 2403
Size: 1066 1337
DraIII CAC_nnn'GTG
Cuts at: 0 520 873 2403
Size: 520 353 1530
Fragments arranged by size:
1530 520 353
EaeI y'GGCC_r
Cuts at: 0 546 875 1329 2403
Size: 546 329 454 1074
Fragments arranged by size:
1074 546 454 329
EarI CTCTTCn'nnn_
Cuts at: 0 1484 2291 2306 2403
Size: 1484 807 15 97
Fragments arranged by size:
1484 807 97 15
EcoICRI GAG'CTC
Cuts at: 0 185 789 2403
Size: 185 604 1614
Fragments arranged by size:
1614 604 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 416 669 805 1022 1307 2002 2253
Size: 319 97 253 136 217 285 695 251
Cuts at: 2253 2403
Size: 150
Fragments arranged by size:
695 319 285 253 251 217 150 136
97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 140 2403
Size: 140 2263
EcoO109I rG'GnC_Cy
Cuts at: 0 172 2061 2260 2403
Size: 172 1889 199 143
Fragments arranged by size:
1889 199 172 143
EcoRI G'AATT_C
Cuts at: 0 1417 2403
Size: 1417 986
EcoRV GAT'ATC
Cuts at: 0 1551 2403
Size: 1551 852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2148 2403
Size: 2148 255
FspI TGC'GCA
Cuts at: 0 1471 2403
Size: 1471 932
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 2094 2126 2127 2159 2403
Size: 487 32 1575 32 1 32 244
Fragments arranged by size:
1575 487 244 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1380 2403
Size: 1380 1023
HindIII A'AGCT_T
Cuts at: 0 1790 2403
Size: 1790 613
HpaI GTT'AAC
Cuts at: 0 1380 2403
Size: 1380 1023
MfeI C'AATT_G
Cuts at: 0 1256 2403
Size: 1256 1147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 2403
Size: 447 1956
MscI TGG'CCA
Cuts at: 0 548 877 1331 2403
Size: 548 329 454 1072
Fragments arranged by size:
1072 548 454 329
MslI CAynn'nnrTG
Cuts at: 0 539 755 776 1028 1300 1406 1625 1840
Size: 539 216 21 252 272 106 219 215
Cuts at: 1840 1856 2403
Size: 16 547
Fragments arranged by size:
547 539 272 252 219 216 215 106
21 16
MspA1I CmG'CkG
Cuts at: 0 51 2377 2403
Size: 51 2326 26
Fragments arranged by size:
2326 51 26
NcoI C'CATG_G
Cuts at: 0 1346 1737 2403
Size: 1346 391 666
Fragments arranged by size:
1346 666 391
NsiI A_TGCA'T
Cuts at: 0 5 773 2403
Size: 5 768 1630
Fragments arranged by size:
1630 768 5
NspI r_CATG'y
Cuts at: 0 840 2403
Size: 840 1563
PasI CC'CwG_GG
Cuts at: 0 1719 2403
Size: 1719 684
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 1827 2403
Size: 555 1272 576
Fragments arranged by size:
1272 576 555
PmlI CAC'GTG
Cuts at: 0 2076 2403
Size: 2076 327
PpuMI rG'GwC_Cy
Cuts at: 0 172 2061 2403
Size: 172 1889 342
Fragments arranged by size:
1889 342 172
PspOMI G'GGCC_C
Cuts at: 0 2260 2403
Size: 2260 143
PvuII CAG'CTG
Cuts at: 0 51 2403
Size: 51 2352
SacI G_AGCT'C
Cuts at: 0 187 791 2403
Size: 187 604 1612
Fragments arranged by size:
1612 604 187
SanDI GG'GwC_CC
Cuts at: 0 172 2403
Size: 172 2231
SapI GCTCTTCn'nnn_
Cuts at: 0 1484 2403
Size: 1484 919
SfcI C'TryA_G
Cuts at: 0 743 1866 1935 2349 2403
Size: 743 1123 69 414 54
Fragments arranged by size:
1123 743 414 69 54
SmlI C'TyrA_G
Cuts at: 0 1909 2020 2065 2090 2133 2403
Size: 1909 111 45 25 43 270
Fragments arranged by size:
1909 270 111 45 43 25
StuI AGG'CCT
Cuts at: 0 146 2403
Size: 146 2257
StyI C'CwwG_G
Cuts at: 0 560 1346 1737 2403
Size: 560 786 391 666
Fragments arranged by size:
786 666 560 391
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 132 1012 1637 1981 2403
Size: 132 880 625 344 422
Fragments arranged by size:
880 625 422 344 132
TatI w'GTAC_w
Cuts at: 0 368 1129 2203 2403
Size: 368 761 1074 200
Fragments arranged by size:
1074 761 368 200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 261 293 356 388 1535 1567 1969 2001
Size: 261 32 63 32 1147 32 402 32
Cuts at: 2001 2346 2378 2403
Size: 345 32 25
Fragments arranged by size:
1147 402 345 261 63 32 32 32
32 32 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 676 870 2403
Size: 676 194 1533
Fragments arranged by size:
1533 676 194
XmnI GAAnn'nnTTC
Cuts at: 0 1295 2403
Size: 1295 1108
Enzymes that do cut:
AclI AcuI AflII AleI AlfI AlwNI ApaI ApoI
BaeI BanI BanII BbsI BbvCI BfrBI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PflMI PmlI PpuMI PspOMI PvuII SacI SanDI SapI
SfcI SmlI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BcgI BciVI BclI BglI BlpI BmgBI BmtI
BsaI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI FseI FspAI HaeII
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PciI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI