Restriction map for
pF1KB4983
(Linear) (Six-base) MAPSORT of: KIBB4983.seq Check: 2069 from: 1 to: 1527
KIBB4983.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1211 1527
Size: 923 288 316
Fragments arranged by size:
923 316 288
AlwNI CAG_nnn'CTG
Cuts at: 0 1378 1527
Size: 1378 149
ApaLI G'TGCA_C
Cuts at: 0 1144 1527
Size: 1144 383
ApoI r'AATT_y
Cuts at: 0 354 727 811 960 1007 1037 1258 1527
Size: 354 373 84 149 47 30 221 269
Fragments arranged by size:
373 354 269 221 149 84 47 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 584 617 1527
Size: 584 33 910
Fragments arranged by size:
910 584 33
Bme1580I G_kGCm'C
Cuts at: 0 1148 1527
Size: 1148 379
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 1360 1527
Size: 177 1183 167
Fragments arranged by size:
1183 177 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 173 1400 1527
Size: 30 143 1227 127
Fragments arranged by size:
1227 143 127 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 1373 1403 1527
Size: 146 30 1197 30 124
Fragments arranged by size:
1197 146 124 30 30
BseYI C'CCAG_C
Cuts at: 0 978 1527
Size: 978 549
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 728 1527
Size: 680 48 799
Fragments arranged by size:
799 680 48
BsiHKAI G_wGCw'C
Cuts at: 0 1148 1359 1527
Size: 1148 211 168
Fragments arranged by size:
1148 211 168
Bsp1286I G_dGCh'C
Cuts at: 0 1148 1359 1527
Size: 1148 211 168
Fragments arranged by size:
1148 211 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1495 1527
Size: 1495 32
BstYI r'GATC_y
Cuts at: 0 993 1319 1527
Size: 993 326 208
Fragments arranged by size:
993 326 208
BtgI C'CryG_G
Cuts at: 0 303 545 1527
Size: 303 242 982
Fragments arranged by size:
982 303 242
BtsI GCAGTG_nn'
Cuts at: 0 92 485 1527
Size: 92 393 1042
Fragments arranged by size:
1042 393 92
DraI TTT'AAA
Cuts at: 0 627 810 816 1527
Size: 627 183 6 711
Fragments arranged by size:
711 627 183 6
DraIII CAC_nnn'GTG
Cuts at: 0 229 1527
Size: 229 1298
EaeI y'GGCC_r
Cuts at: 0 32 939 1527
Size: 32 907 588
Fragments arranged by size:
907 588 32
EarI CTCTTCn'nnn_
Cuts at: 0 373 968 1211 1226 1527
Size: 373 595 243 15 301
Fragments arranged by size:
595 373 301 243 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 173 923 1211 1400 1527
Size: 30 143 750 288 189 127
Fragments arranged by size:
750 288 189 143 127 30
EcoRI G'AATT_C
Cuts at: 0 1007 1527
Size: 1007 520
EcoRV GAT'ATC
Cuts at: 0 770 1527
Size: 770 757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1420 1510 1527
Size: 1420 90 17
Fragments arranged by size:
1420 90 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 304 336 1527
Size: 29 275 32 1191
Fragments arranged by size:
1191 275 32 29
HindIII A'AGCT_T
Cuts at: 0 395 1475 1527
Size: 395 1080 52
Fragments arranged by size:
1080 395 52
MfeI C'AATT_G
Cuts at: 0 1158 1527
Size: 1158 369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1486 1527
Size: 732 754 41
Fragments arranged by size:
754 732 41
MscI TGG'CCA
Cuts at: 0 34 1527
Size: 34 1493
MslI CAynn'nnrTG
Cuts at: 0 449 743 1527
Size: 449 294 784
Fragments arranged by size:
784 449 294
NcoI C'CATG_G
Cuts at: 0 303 1527
Size: 303 1224
NdeI CA'TA_TG
Cuts at: 0 296 1527
Size: 296 1231
NspI r_CATG'y
Cuts at: 0 239 1527
Size: 239 1288
PflMI CCAn_nnn'nTGG
Cuts at: 0 1378 1527
Size: 1378 149
PfoI T'CCnGG_A
Cuts at: 0 150 570 1527
Size: 150 420 957
Fragments arranged by size:
957 420 150
PstI C_TGCA'G
Cuts at: 0 87 390 1527
Size: 87 303 1137
Fragments arranged by size:
1137 303 87
SapI GCTCTTCn'nnn_
Cuts at: 0 968 1211 1527
Size: 968 243 316
Fragments arranged by size:
968 316 243
ScaI AGT'ACT
Cuts at: 0 384 1349 1527
Size: 384 965 178
Fragments arranged by size:
965 384 178
SexAI A'CCwGG_T
Cuts at: 0 1371 1527
Size: 1371 156
SfcI C'TryA_G
Cuts at: 0 70 83 386 608 1527
Size: 70 13 303 222 919
Fragments arranged by size:
919 303 222 70 13
StyI C'CwwG_G
Cuts at: 0 29 303 1323 1527
Size: 29 274 1020 204
Fragments arranged by size:
1020 274 204 29
SwaI ATTT'AAAT
Cuts at: 0 816 1527
Size: 816 711
TatI w'GTAC_w
Cuts at: 0 20 382 921 1244 1347 1527
Size: 20 362 539 323 103 180
Fragments arranged by size:
539 362 323 180 103 20
XbaI T'CTAG_A
Cuts at: 0 322 1527
Size: 322 1205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 1527
Size: 301 1226
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI BaeI Bme1580I BmrI BpmI
BsaXI BseYI BsgI BsiHKAI Bsp1286I BstAPI BstYI BtgI
BtsI DraI DraIII EaeI EarI Eco57MI EcoRI EcoRV
FalI Hin4I HindIII MfeI MmeI MscI MslI NcoI
NdeI NspI PflMI PfoI PstI SapI ScaI SexAI
SfcI StyI SwaI TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MluI MspA1I
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI TaqII
TstI Tth111I XhoI XmaI XmnI ZraI