Restriction map for
pF1KB4985
(Linear) (Six-base) MAPSORT of: KIBB4985.seq Check: 8436 from: 1 to: 1605
KIBB4985.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 943 1605
Size: 943 662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1544 1605
Size: 1544 61
AflII C'TTAA_G
Cuts at: 0 847 1605
Size: 847 758
AflIII A'CryG_T
Cuts at: 0 1581 1605
Size: 1581 24
AhdI GACnn_n'nnGTC
Cuts at: 0 71 1605
Size: 71 1534
AlwNI CAG_nnn'CTG
Cuts at: 0 46 1605
Size: 46 1559
ApoI r'AATT_y
Cuts at: 0 149 460 707 1142 1605
Size: 149 311 247 435 463
Fragments arranged by size:
463 435 311 247 149
AseI AT'TA_AT
Cuts at: 0 883 1605
Size: 883 722
AvaI C'yCGr_G
Cuts at: 0 761 1605
Size: 761 844
BanI G'GyrC_C
Cuts at: 0 91 199 1129 1447 1569 1605
Size: 91 108 930 318 122 36
Fragments arranged by size:
930 318 122 108 91 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 1073 1605
Size: 1073 532
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1026 1060 1605
Size: 1026 34 545
Fragments arranged by size:
1026 545 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 726 1605
Size: 726 879
BclI T'GATC_A
Cuts at: 0 438 1005 1590 1605
Size: 438 567 585 15
Fragments arranged by size:
585 567 438 15
BglI GCCn_nnn'nGGC
Cuts at: 0 403 1605
Size: 403 1202
Bme1580I G_kGCm'C
Cuts at: 0 493 1605
Size: 493 1112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1605
Size: 607 998
Bpu10I CC'TnA_GC
Cuts at: 0 1393 1605
Size: 1393 212
BsaHI Gr'CG_yC
Cuts at: 0 4 1605
Size: 4 1601
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 1605
Size: 98 30 1477
Fragments arranged by size:
1477 98 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1241 1605
Size: 1241 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1394 1538 1605
Size: 1394 144 67
Fragments arranged by size:
1394 144 67
BsiHKAI G_wGCw'C
Cuts at: 0 124 1605
Size: 124 1481
Bsp1286I G_dGCh'C
Cuts at: 0 124 493 1605
Size: 124 369 1112
Fragments arranged by size:
1112 369 124
BspHI T'CATG_A
Cuts at: 0 1055 1605
Size: 1055 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 943 1605
Size: 943 662
BsrDI GCAATG_nn'
Cuts at: 0 1165 1310 1605
Size: 1165 145 295
Fragments arranged by size:
1165 295 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 790 949 1605
Size: 790 159 656
Fragments arranged by size:
790 656 159
BstEII G'GTnAC_C
Cuts at: 0 186 1605
Size: 186 1419
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 1605
Size: 141 1464
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 81 1605
Size: 81 1524
BtsI GCAGTG_nn'
Cuts at: 0 168 1421 1605
Size: 168 1253 184
Fragments arranged by size:
1253 184 168
EarI CTCTTCn'nnn_
Cuts at: 0 47 1605
Size: 47 1558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1544 1605
Size: 607 937 61
Fragments arranged by size:
937 607 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1102 1605
Size: 1102 503
EcoO109I rG'GnC_Cy
Cuts at: 0 130 1605
Size: 130 1475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 1605
Size: 141 1464
FspI TGC'GCA
Cuts at: 0 1193 1605
Size: 1193 412
HaeII r_GCGC'y
Cuts at: 0 421 1605
Size: 421 1184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 1605
Size: 57 32 1516
Fragments arranged by size:
1516 57 32
HincII GTy'rAC
Cuts at: 0 27 228 774 1605
Size: 27 201 546 831
Fragments arranged by size:
831 546 201 27
HindIII A'AGCT_T
Cuts at: 0 1024 1605
Size: 1024 581
HpaI GTT'AAC
Cuts at: 0 27 1605
Size: 27 1578
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 638 1605
Size: 367 271 967
Fragments arranged by size:
967 367 271
MspA1I CmG'CkG
Cuts at: 0 1522 1605
Size: 1522 83
NspI r_CATG'y
Cuts at: 0 808 943 1605
Size: 808 135 662
Fragments arranged by size:
808 662 135
PpuMI rG'GwC_Cy
Cuts at: 0 130 1605
Size: 130 1475
PsiI TTA'TAA
Cuts at: 0 890 1605
Size: 890 715
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 542 574 1605
Size: 542 32 1031
Fragments arranged by size:
1031 542 32
PstI C_TGCA'G
Cuts at: 0 435 1605
Size: 435 1170
PvuII CAG'CTG
Cuts at: 0 1522 1605
Size: 1522 83
SanDI GG'GwC_CC
Cuts at: 0 130 1605
Size: 130 1475
SexAI A'CCwGG_T
Cuts at: 0 1379 1605
Size: 1379 226
SfcI C'TryA_G
Cuts at: 0 431 548 1605
Size: 431 117 1057
Fragments arranged by size:
1057 431 117
SmaI CCC'GGG
Cuts at: 0 763 1605
Size: 763 842
SmlI C'TyrA_G
Cuts at: 0 847 1605
Size: 847 758
SphI G_CATG'C
Cuts at: 0 943 1605
Size: 943 662
SspI AAT'ATT
Cuts at: 0 483 1605
Size: 483 1122
StyI C'CwwG_G
Cuts at: 0 125 1098 1605
Size: 125 973 507
Fragments arranged by size:
973 507 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1025 1587 1605
Size: 1025 562 18
Fragments arranged by size:
1025 562 18
XbaI T'CTAG_A
Cuts at: 0 1210 1605
Size: 1210 395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 1428 1458 1605
Size: 108 1320 30 147
Fragments arranged by size:
1320 147 108 30
XmaI C'CCGG_G
Cuts at: 0 761 1605
Size: 761 844
XmnI GAAnn'nnTTC
Cuts at: 0 557 842 1487 1605
Size: 557 285 645 118
Fragments arranged by size:
645 557 285 118
Enzymes that do cut:
AarI AcuI AflII AflIII AhdI AlwNI ApoI AseI
AvaI BanI BbsI BcgI BciVI BclI BglI Bme1580I
BpmI Bpu10I BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrDI BstAPI BstEII BstXI BtgZI BtsI
EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII HindIII HpaI MmeI MspA1I NspI PpuMI PsiI
PsrI PstI PvuII SanDI SexAI SfcI SmaI SmlI
SphI SspI StyI TaqII XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BanII BbeI BbvCI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BseYI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoRI EcoRV FseI FspAI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI StuI SwaI TatI
TstI Tth111I XhoI ZraI