Restriction map for
pF1KB4988
(Linear) (Six-base) MAPSORT of: KIBB4988.seq Check: 4248 from: 1 to: 759
KIBB4988.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 672 759
Size: 672 87
ApoI r'AATT_y
Cuts at: 0 446 759
Size: 446 313
BanII G_rGCy'C
Cuts at: 0 186 734 759
Size: 186 548 25
Fragments arranged by size:
548 186 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 759
Size: 93 666
BbvCI CC'TCA_GC
Cuts at: 0 581 759
Size: 581 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 593 759
Size: 559 34 166
Fragments arranged by size:
559 166 34
BclI T'GATC_A
Cuts at: 0 167 759
Size: 167 592
BfrBI ATG'CAT
Cuts at: 0 630 759
Size: 630 129
BglI GCCn_nnn'nGGC
Cuts at: 0 11 649 759
Size: 11 638 110
Fragments arranged by size:
638 110 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 474 759
Size: 474 285
BmtI G_CTAG'C
Cuts at: 0 404 759
Size: 404 355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 527 759
Size: 527 232
Bpu10I CC'TnA_GC
Cuts at: 0 581 759
Size: 581 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 83 113 759
Size: 83 30 646
Fragments arranged by size:
646 83 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 724 759
Size: 12 712 35
Fragments arranged by size:
712 35 12
BseYI C'CCAG_C
Cuts at: 0 672 759
Size: 672 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 723 759
Size: 723 36
BsiHKAI G_wGCw'C
Cuts at: 0 734 759
Size: 734 25
BsmI GAATG_Cn'
Cuts at: 0 353 759
Size: 353 406
Bsp1286I G_dGCh'C
Cuts at: 0 186 734 759
Size: 186 548 25
Fragments arranged by size:
548 186 25
BspHI T'CATG_A
Cuts at: 0 623 759
Size: 623 136
BsrGI T'GTAC_A
Cuts at: 0 143 420 759
Size: 143 277 339
Fragments arranged by size:
339 277 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 80 759
Size: 80 679
BstYI r'GATC_y
Cuts at: 0 717 759
Size: 717 42
EcoICRI GAG'CTC
Cuts at: 0 732 759
Size: 732 27
EcoRI G'AATT_C
Cuts at: 0 446 759
Size: 446 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 83 115 702 734 759
Size: 83 32 587 32 25
Fragments arranged by size:
587 83 32 32 25
MfeI C'AATT_G
Cuts at: 0 540 759
Size: 540 219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 759
Size: 593 166
MslI CAynn'nnrTG
Cuts at: 0 440 759
Size: 440 319
MspA1I CmG'CkG
Cuts at: 0 70 432 669 759
Size: 70 362 237 90
Fragments arranged by size:
362 237 90 70
NheI G'CTAG_C
Cuts at: 0 400 759
Size: 400 359
NsiI A_TGCA'T
Cuts at: 0 632 759
Size: 632 127
NspI r_CATG'y
Cuts at: 0 634 681 759
Size: 634 47 78
Fragments arranged by size:
634 78 47
PfoI T'CCnGG_A
Cuts at: 0 227 759
Size: 227 532
PstI C_TGCA'G
Cuts at: 0 526 759
Size: 526 233
PvuII CAG'CTG
Cuts at: 0 70 432 669 759
Size: 70 362 237 90
Fragments arranged by size:
362 237 90 70
SacI G_AGCT'C
Cuts at: 0 734 759
Size: 734 25
SfcI C'TryA_G
Cuts at: 0 161 176 522 759
Size: 161 15 346 237
Fragments arranged by size:
346 237 161 15
SphI G_CATG'C
Cuts at: 0 681 759
Size: 681 78
TatI w'GTAC_w
Cuts at: 0 143 420 759
Size: 143 277 339
Fragments arranged by size:
339 277 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 759
Size: 33 726
Enzymes that do cut:
AlwNI ApoI BanII BbsI BbvCI BcgI BclI BfrBI
BglI BmrI BmtI BplI Bpu10I BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrGI BstAPI BstYI
EcoICRI EcoRI Hin4I MfeI MmeI MslI MspA1I NheI
NsiI NspI PfoI PstI PvuII SacI SfcI SphI
TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BciVI BglII BlpI Bme1580I BmgBI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI