Restriction map for pF1KB4989
 (Linear) (Six-base) MAPSORT of: KIBB4989.seq  Check: 6856  from: 1  to: 783



KIBB4989.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      23     783 
   Size:         23     760
 
AccI GT'mk_AC

Cuts at:      0     436     783 
   Size:        436     347
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     664     783 
   Size:        310     354     119

  Fragments arranged by size: 

                 354     310     119
 
AflII C'TTAA_G

Cuts at:      0     367     783 
   Size:        367     416
 
AlwNI CAG_nnn'CTG

Cuts at:      0     288     675     783 
   Size:        288     387     108

  Fragments arranged by size: 

                 387     288     108
 
ApoI r'AATT_y

Cuts at:      0     258     304     525     538     783 
   Size:        258      46     221      13     245

  Fragments arranged by size: 

                 258     245     221      46      13
 
AseI AT'TA_AT

Cuts at:      0     683     783 
   Size:        683     100
 
BanII G_rGCy'C

Cuts at:      0     128     783 
   Size:        128     655
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319     783 
   Size:        319     464
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     604     783 
   Size:        604     179
 
BclI T'GATC_A

Cuts at:      0     594     783 
   Size:        594     189
 
BglII A'GATC_T

Cuts at:      0      53     783 
   Size:         53     730
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     783 
   Size:        112     671
 
BsaAI yAC'GTr

Cuts at:      0     240     783 
   Size:        240     543
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     376     783 
   Size:        376     407
 
BseYI C'CCAG_C

Cuts at:      0     772     783 
   Size:        772      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     783 
   Size:        538     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     783 
   Size:        128     655
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     783 
   Size:        128     655
 
BspHI T'CATG_A

Cuts at:      0     308     783 
   Size:        308     475
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      23     395     783 
   Size:         23     372     388

  Fragments arranged by size: 

                 388     372      23
 
BsrDI GCAATG_nn'

Cuts at:      0     612     783 
   Size:        612     171
 
BstEII G'GTnAC_C

Cuts at:      0      85     783 
   Size:         85     698
 
BstYI r'GATC_y

Cuts at:      0      53     648     672     750     783 
   Size:         53     595      24      78      33

  Fragments arranged by size: 

                 595      78      53      33      24
 
BstZ17I GTA'TAC

Cuts at:      0     437     783 
   Size:        437     346
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     540     575     783 
   Size:        540      35     208

  Fragments arranged by size: 

                 540     208      35
 
EaeI y'GGCC_r

Cuts at:      0     500     783 
   Size:        500     283
 
EarI CTCTTCn'nnn_

Cuts at:      0     248     277     783 
   Size:        248      29     506

  Fragments arranged by size: 

                 506     248      29
 
EcoICRI GAG'CTC

Cuts at:      0     126     783 
   Size:        126     657
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     310     664     783 
   Size:        112     198     354     119

  Fragments arranged by size: 

                 354     198     119     112
 
EcoO109I rG'GnC_Cy

Cuts at:      0     384     783 
   Size:        384     399
 
EcoRI G'AATT_C

Cuts at:      0     304     783 
   Size:        304     479
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     704     783 
   Size:        704      79
 
MscI TGG'CCA

Cuts at:      0     502     783 
   Size:        502     281
 
MspA1I CmG'CkG

Cuts at:      0     288     783 
   Size:        288     495
 
PfoI T'CCnGG_A

Cuts at:      0     119     783 
   Size:        119     664
 
PpuMI rG'GwC_Cy

Cuts at:      0     384     783 
   Size:        384     399
 
PstI C_TGCA'G

Cuts at:      0      20     110     783 
   Size:         20      90     673

  Fragments arranged by size: 

                 673      90      20
 
PvuII CAG'CTG

Cuts at:      0     288     783 
   Size:        288     495
 
SacI G_AGCT'C

Cuts at:      0     128     783 
   Size:        128     655
 
SapI GCTCTTCn'nnn_

Cuts at:      0     277     783 
   Size:        277     506
 
SfcI C'TryA_G

Cuts at:      0      16     106     783 
   Size:         16      90     677

  Fragments arranged by size: 

                 677      90      16
 
SmlI C'TyrA_G

Cuts at:      0     367     783 
   Size:        367     416
 
SspI AAT'ATT

Cuts at:      0     690     783 
   Size:        690      93
 
XmnI GAAnn'nnTTC

Cuts at:      0     568     783 
   Size:        568     215
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII    AlwNI     ApoI     AseI    BanII
     BbsI    BciVI     BclI    BglII     BpmI    BsaAI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI   BstEII    BstYI
  BstZ17I    CspCI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI
     MmeI     MscI   MspA1I     PfoI    PpuMI     PstI    PvuII     SacI
     SapI     SfcI     SmlI     SspI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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