Restriction map for
pF1KB4989
(Linear) (Six-base) MAPSORT of: KIBB4989.seq Check: 6856 from: 1 to: 783
KIBB4989.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 23 783
Size: 23 760
AccI GT'mk_AC
Cuts at: 0 436 783
Size: 436 347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 664 783
Size: 310 354 119
Fragments arranged by size:
354 310 119
AflII C'TTAA_G
Cuts at: 0 367 783
Size: 367 416
AlwNI CAG_nnn'CTG
Cuts at: 0 288 675 783
Size: 288 387 108
Fragments arranged by size:
387 288 108
ApoI r'AATT_y
Cuts at: 0 258 304 525 538 783
Size: 258 46 221 13 245
Fragments arranged by size:
258 245 221 46 13
AseI AT'TA_AT
Cuts at: 0 683 783
Size: 683 100
BanII G_rGCy'C
Cuts at: 0 128 783
Size: 128 655
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 783
Size: 319 464
BciVI GTATCCnnnnn_n'
Cuts at: 0 604 783
Size: 604 179
BclI T'GATC_A
Cuts at: 0 594 783
Size: 594 189
BglII A'GATC_T
Cuts at: 0 53 783
Size: 53 730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 783
Size: 112 671
BsaAI yAC'GTr
Cuts at: 0 240 783
Size: 240 543
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 783
Size: 376 407
BseYI C'CCAG_C
Cuts at: 0 772 783
Size: 772 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 783
Size: 538 245
BsiHKAI G_wGCw'C
Cuts at: 0 128 783
Size: 128 655
Bsp1286I G_dGCh'C
Cuts at: 0 128 783
Size: 128 655
BspHI T'CATG_A
Cuts at: 0 308 783
Size: 308 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 23 395 783
Size: 23 372 388
Fragments arranged by size:
388 372 23
BsrDI GCAATG_nn'
Cuts at: 0 612 783
Size: 612 171
BstEII G'GTnAC_C
Cuts at: 0 85 783
Size: 85 698
BstYI r'GATC_y
Cuts at: 0 53 648 672 750 783
Size: 53 595 24 78 33
Fragments arranged by size:
595 78 53 33 24
BstZ17I GTA'TAC
Cuts at: 0 437 783
Size: 437 346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 540 575 783
Size: 540 35 208
Fragments arranged by size:
540 208 35
EaeI y'GGCC_r
Cuts at: 0 500 783
Size: 500 283
EarI CTCTTCn'nnn_
Cuts at: 0 248 277 783
Size: 248 29 506
Fragments arranged by size:
506 248 29
EcoICRI GAG'CTC
Cuts at: 0 126 783
Size: 126 657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 310 664 783
Size: 112 198 354 119
Fragments arranged by size:
354 198 119 112
EcoO109I rG'GnC_Cy
Cuts at: 0 384 783
Size: 384 399
EcoRI G'AATT_C
Cuts at: 0 304 783
Size: 304 479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 783
Size: 704 79
MscI TGG'CCA
Cuts at: 0 502 783
Size: 502 281
MspA1I CmG'CkG
Cuts at: 0 288 783
Size: 288 495
PfoI T'CCnGG_A
Cuts at: 0 119 783
Size: 119 664
PpuMI rG'GwC_Cy
Cuts at: 0 384 783
Size: 384 399
PstI C_TGCA'G
Cuts at: 0 20 110 783
Size: 20 90 673
Fragments arranged by size:
673 90 20
PvuII CAG'CTG
Cuts at: 0 288 783
Size: 288 495
SacI G_AGCT'C
Cuts at: 0 128 783
Size: 128 655
SapI GCTCTTCn'nnn_
Cuts at: 0 277 783
Size: 277 506
SfcI C'TryA_G
Cuts at: 0 16 106 783
Size: 16 90 677
Fragments arranged by size:
677 90 16
SmlI C'TyrA_G
Cuts at: 0 367 783
Size: 367 416
SspI AAT'ATT
Cuts at: 0 690 783
Size: 690 93
XmnI GAAnn'nnTTC
Cuts at: 0 568 783
Size: 568 215
Enzymes that do cut:
AarI AccI AcuI AflII AlwNI ApoI AseI BanII
BbsI BciVI BclI BglII BpmI BsaAI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstEII BstYI
BstZ17I CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
MmeI MscI MspA1I PfoI PpuMI PstI PvuII SacI
SapI SfcI SmlI SspI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI Bsu36I BtgI BtgZI BtsI ClaI DraI
DraIII DrdI EagI EciI EcoNI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI