(Linear) (Six-base) MAPSORT of: KIBB4989.seq Check: 6856 from: 1 to: 783 KIBB4989.orf With 182 enzymes: SgfI * April 24, 2009 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 23 783 Size: 23 760 AccI GT'mk_AC Cuts at: 0 436 783 Size: 436 347 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 664 783 Size: 310 354 119 Fragments arranged by size: 354 310 119 AflII C'TTAA_G Cuts at: 0 367 783 Size: 367 416 AlwNI CAG_nnn'CTG Cuts at: 0 288 675 783 Size: 288 387 108 Fragments arranged by size: 387 288 108 ApoI r'AATT_y Cuts at: 0 258 304 525 538 783 Size: 258 46 221 13 245 Fragments arranged by size: 258 245 221 46 13 AseI AT'TA_AT Cuts at: 0 683 783 Size: 683 100 BanII G_rGCy'C Cuts at: 0 128 783 Size: 128 655 BbsI GAAGACnn'nnnn_ Cuts at: 0 319 783 Size: 319 464 BciVI GTATCCnnnnn_n' Cuts at: 0 604 783 Size: 604 179 BclI T'GATC_A Cuts at: 0 594 783 Size: 594 189 BglII A'GATC_T Cuts at: 0 53 783 Size: 53 730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 783 Size: 112 671 BsaAI yAC'GTr Cuts at: 0 240 783 Size: 240 543 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 376 783 Size: 376 407 BseYI C'CCAG_C Cuts at: 0 772 783 Size: 772 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 538 783 Size: 538 245 BsiHKAI G_wGCw'C Cuts at: 0 128 783 Size: 128 655 Bsp1286I G_dGCh'C Cuts at: 0 128 783 Size: 128 655 BspHI T'CATG_A Cuts at: 0 308 783 Size: 308 475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 23 395 783 Size: 23 372 388 Fragments arranged by size: 388 372 23 BsrDI GCAATG_nn' Cuts at: 0 612 783 Size: 612 171 BstEII G'GTnAC_C Cuts at: 0 85 783 Size: 85 698 BstYI r'GATC_y Cuts at: 0 53 648 672 750 783 Size: 53 595 24 78 33 Fragments arranged by size: 595 78 53 33 24 BstZ17I GTA'TAC Cuts at: 0 437 783 Size: 437 346 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 540 575 783 Size: 540 35 208 Fragments arranged by size: 540 208 35 EaeI y'GGCC_r Cuts at: 0 500 783 Size: 500 283 EarI CTCTTCn'nnn_ Cuts at: 0 248 277 783 Size: 248 29 506 Fragments arranged by size: 506 248 29 EcoICRI GAG'CTC Cuts at: 0 126 783 Size: 126 657 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 310 664 783 Size: 112 198 354 119 Fragments arranged by size: 354 198 119 112 EcoO109I rG'GnC_Cy Cuts at: 0 384 783 Size: 384 399 EcoRI G'AATT_C Cuts at: 0 304 783 Size: 304 479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 704 783 Size: 704 79 MscI TGG'CCA Cuts at: 0 502 783 Size: 502 281 MspA1I CmG'CkG Cuts at: 0 288 783 Size: 288 495 PfoI T'CCnGG_A Cuts at: 0 119 783 Size: 119 664 PpuMI rG'GwC_Cy Cuts at: 0 384 783 Size: 384 399 PstI C_TGCA'G Cuts at: 0 20 110 783 Size: 20 90 673 Fragments arranged by size: 673 90 20 PvuII CAG'CTG Cuts at: 0 288 783 Size: 288 495 SacI G_AGCT'C Cuts at: 0 128 783 Size: 128 655 SapI GCTCTTCn'nnn_ Cuts at: 0 277 783 Size: 277 506 SfcI C'TryA_G Cuts at: 0 16 106 783 Size: 16 90 677 Fragments arranged by size: 677 90 16 SmlI C'TyrA_G Cuts at: 0 367 783 Size: 367 416 SspI AAT'ATT Cuts at: 0 690 783 Size: 690 93 XmnI GAAnn'nnTTC Cuts at: 0 568 783 Size: 568 215 Enzymes that do cut: AarI AccI AcuI AflII AlwNI ApoI AseI BanII BbsI BciVI BclI BglII BpmI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstEII BstYI BstZ17I CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI MmeI MscI MspA1I PfoI PpuMI PstI PvuII SacI SapI SfcI SmlI SspI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI