Restriction map for
pF1KB4990
(Linear) (Six-base) MAPSORT of: KIBB4990.seq Check: 3138 from: 1 to: 1479
KIBB4990.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1261 1479
Size: 1261 218
AfeI AGC'GCT
Cuts at: 0 29 1479
Size: 29 1450
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 952 1479
Size: 952 527
AlwNI CAG_nnn'CTG
Cuts at: 0 261 1474 1479
Size: 261 1213 5
Fragments arranged by size:
1213 261 5
ApaI G_GGCC'C
Cuts at: 0 18 855 1479
Size: 18 837 624
Fragments arranged by size:
837 624 18
AvaI C'yCGr_G
Cuts at: 0 395 1479
Size: 395 1084
BamHI G'GATC_C
Cuts at: 0 399 663 1479
Size: 399 264 816
Fragments arranged by size:
816 399 264
BanI G'GyrC_C
Cuts at: 0 882 1021 1479
Size: 882 139 458
Fragments arranged by size:
882 458 139
BanII G_rGCy'C
Cuts at: 0 18 239 392 764 855 946 1479
Size: 18 221 153 372 91 91 533
Fragments arranged by size:
533 372 221 153 91 91 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 1249 1479
Size: 1249 230
BbvCI CC'TCA_GC
Cuts at: 0 354 359 907 1479
Size: 354 5 548 572
Fragments arranged by size:
572 548 354 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1190 1224 1479
Size: 1190 34 255
Fragments arranged by size:
1190 255 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1129 1479
Size: 1129 350
BglII A'GATC_T
Cuts at: 0 548 1479
Size: 548 931
BlpI GC'TnA_GC
Cuts at: 0 940 1479
Size: 940 539
Bme1580I G_kGCm'C
Cuts at: 0 18 855 887 1479
Size: 18 837 32 592
Fragments arranged by size:
837 592 32 18
BmtI G_CTAG'C
Cuts at: 0 1046 1479
Size: 1046 433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 145 157 406 435 756 994 1225
Size: 112 33 12 249 29 321 238 231
Cuts at: 1225 1479
Size: 254
Fragments arranged by size:
321 254 249 238 231 112 33 29
12
Bpu10I CC'TnA_GC
Cuts at: 0 354 359 907 1479
Size: 354 5 548 572
Fragments arranged by size:
572 548 354 5
BsaHI Gr'CG_yC
Cuts at: 0 1109 1444 1479
Size: 1109 335 35
Fragments arranged by size:
1109 335 35
BsaWI w'CCGG_w
Cuts at: 0 153 302 1479
Size: 153 149 1177
Fragments arranged by size:
1177 153 149
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 526 1479
Size: 526 953
BseYI C'CCAG_C
Cuts at: 0 764 945 1479
Size: 764 181 534
Fragments arranged by size:
764 534 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1184 1321 1479
Size: 987 197 137 158
Fragments arranged by size:
987 197 158 137
BsiHKAI G_wGCw'C
Cuts at: 0 239 392 1479
Size: 239 153 1087
Fragments arranged by size:
1087 239 153
BsmI GAATG_Cn'
Cuts at: 0 461 1479
Size: 461 1018
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1120 1479
Size: 1120 359
Bsp1286I G_dGCh'C
Cuts at: 0 18 239 392 764 855 887 946 1479
Size: 18 221 153 372 91 32 59 533
Fragments arranged by size:
533 372 221 153 91 59 32 18
BspEI T'CCGG_A
Cuts at: 0 153 302 1479
Size: 153 149 1177
Fragments arranged by size:
1177 153 149
BspHI T'CATG_A
Cuts at: 0 656 1479
Size: 656 823
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 1163 1175 1479
Size: 167 996 12 304
Fragments arranged by size:
996 304 167 12
BsrBI CCG'CTC
Cuts at: 0 783 1284 1479
Size: 783 501 195
Fragments arranged by size:
783 501 195
BsrDI GCAATG_nn'
Cuts at: 0 1052 1479
Size: 1052 427
BsrFI r'CCGG_y
Cuts at: 0 1424 1479
Size: 1424 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 864 1479
Size: 864 615
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 687 1059 1479
Size: 90 597 372 420
Fragments arranged by size:
597 420 372 90
BstYI r'GATC_y
Cuts at: 0 68 399 548 663 1304 1479
Size: 68 331 149 115 641 175
Fragments arranged by size:
641 331 175 149 115 68
Bsu36I CC'TnA_GG
Cuts at: 0 519 1079 1316 1479
Size: 519 560 237 163
Fragments arranged by size:
560 519 237 163
BtgI C'CryG_G
Cuts at: 0 827 1479
Size: 827 652
BtsI GCAGTG_nn'
Cuts at: 0 761 956 1037 1448 1479
Size: 761 195 81 411 31
Fragments arranged by size:
761 411 195 81 31
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 903 938 1479
Size: 903 35 541
Fragments arranged by size:
903 541 35
EarI CTCTTCn'nnn_
Cuts at: 0 13 25 773 1479
Size: 13 12 748 706
Fragments arranged by size:
748 706 13 12
EcoICRI GAG'CTC
Cuts at: 0 237 390 1479
Size: 237 153 1089
Fragments arranged by size:
1089 237 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 145 157 406 435 756 994 1225
Size: 112 33 12 249 29 321 238 231
Cuts at: 1225 1261 1479
Size: 36 218
Fragments arranged by size:
321 249 238 231 218 112 36 33
29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 658 1479
Size: 658 821
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 707 851 852 984 1146 1233
Size: 14 1 692 144 1 132 162 87
Cuts at: 1233 1479
Size: 246
Fragments arranged by size:
692 246 162 144 132 87 14 1
1
FspI TGC'GCA
Cuts at: 0 616 1153 1171 1479
Size: 616 537 18 308
Fragments arranged by size:
616 537 308 18
HaeII r_GCGC'y
Cuts at: 0 31 91 1124 1479
Size: 31 60 1033 355
Fragments arranged by size:
1033 355 60 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 299 331 352 384 487 519 1479
Size: 299 32 21 32 103 32 960
Fragments arranged by size:
960 299 103 32 32 32 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 1479
Size: 1214 265
MslI CAynn'nnrTG
Cuts at: 0 1102 1479
Size: 1102 377
MspA1I CmG'CkG
Cuts at: 0 312 358 406 768 861 1089 1161 1474
Size: 312 46 48 362 93 228 72 313
Cuts at: 1474 1479
Size: 5
Fragments arranged by size:
362 313 312 228 93 72 48 46
5
NcoI C'CATG_G
Cuts at: 0 827 1479
Size: 827 652
NheI G'CTAG_C
Cuts at: 0 1042 1479
Size: 1042 437
NspI r_CATG'y
Cuts at: 0 49 903 1479
Size: 49 854 576
Fragments arranged by size:
854 576 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 894 1479
Size: 894 585
PfoI T'CCnGG_A
Cuts at: 0 596 1479
Size: 596 883
PpuMI rG'GwC_Cy
Cuts at: 0 984 1479
Size: 984 495
PshAI GACnn'nnGTC
Cuts at: 0 1112 1479
Size: 1112 367
PspOMI G'GGCC_C
Cuts at: 0 14 851 1479
Size: 14 837 628
Fragments arranged by size:
837 628 14
PstI C_TGCA'G
Cuts at: 0 773 860 1256 1479
Size: 773 87 396 223
Fragments arranged by size:
773 396 223 87
PvuII CAG'CTG
Cuts at: 0 312 358 768 861 1089 1161 1474 1479
Size: 312 46 410 93 228 72 313 5
Fragments arranged by size:
410 313 312 228 93 72 46 5
SacI G_AGCT'C
Cuts at: 0 239 392 1479
Size: 239 153 1087
Fragments arranged by size:
1087 239 153
SapI GCTCTTCn'nnn_
Cuts at: 0 773 1479
Size: 773 706
SfcI C'TryA_G
Cuts at: 0 769 856 1252 1479
Size: 769 87 396 227
Fragments arranged by size:
769 396 227 87
SmaI CCC'GGG
Cuts at: 0 397 1479
Size: 397 1082
StuI AGG'CCT
Cuts at: 0 442 1479
Size: 442 1037
StyI C'CwwG_G
Cuts at: 0 83 287 827 1479
Size: 83 204 540 652
Fragments arranged by size:
652 540 204 83
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1278 1310 1479
Size: 1278 32 169
Fragments arranged by size:
1278 169 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 684 753 1101 1479
Size: 285 399 69 348 378
Fragments arranged by size:
399 378 348 285 69
XmaI C'CCGG_G
Cuts at: 0 395 1479
Size: 395 1084
XmnI GAAnn'nnTTC
Cuts at: 0 1185 1479
Size: 1185 294
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmtI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI
CspCI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I MmeI MslI MspA1I NcoI NheI NspI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SapI
SfcI SmaI StuI StyI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BclI BfrBI BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XhoI ZraI