Restriction map for
pF1KB4991
(Linear) (Six-base) MAPSORT of: KIBB4991.seq Check: 5719 from: 1 to: 933
KIBB4991.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 346 933
Size: 346 587
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 536 933
Size: 352 184 397
Fragments arranged by size:
397 352 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 933
Size: 115 818
ApoI r'AATT_y
Cuts at: 0 310 933
Size: 310 623
AvaI C'yCGr_G
Cuts at: 0 75 933
Size: 75 858
BanI G'GyrC_C
Cuts at: 0 455 933
Size: 455 478
BanII G_rGCy'C
Cuts at: 0 669 792 933
Size: 669 123 141
Fragments arranged by size:
669 141 123
BbeI G_GCGC'C
Cuts at: 0 459 933
Size: 459 474
BclI T'GATC_A
Cuts at: 0 701 933
Size: 701 232
BglI GCCn_nnn'nGGC
Cuts at: 0 796 933
Size: 796 137
BmrI ACTGGGnnnn_n'
Cuts at: 0 752 933
Size: 752 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 767 933
Size: 495 272 166
Fragments arranged by size:
495 272 166
BsaAI yAC'GTr
Cuts at: 0 366 933
Size: 366 567
BsaHI Gr'CG_yC
Cuts at: 0 456 933
Size: 456 477
BsaWI w'CCGG_w
Cuts at: 0 317 872 933
Size: 317 555 61
Fragments arranged by size:
555 317 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 779 809 933
Size: 779 30 124
Fragments arranged by size:
779 124 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 96 677 933
Size: 93 3 581 256
Fragments arranged by size:
581 256 93 3
BsiEI CG_ry'CG
Cuts at: 0 29 346 933
Size: 29 317 587
Fragments arranged by size:
587 317 29
BsiHKAI G_wGCw'C
Cuts at: 0 263 933
Size: 263 670
Bsp1286I G_dGCh'C
Cuts at: 0 263 669 792 933
Size: 263 406 123 141
Fragments arranged by size:
406 263 141 123
BspEI T'CCGG_A
Cuts at: 0 872 933
Size: 872 61
BspHI T'CATG_A
Cuts at: 0 704 933
Size: 704 229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 647 933
Size: 647 286
BsrBI CCG'CTC
Cuts at: 0 86 933
Size: 86 847
BsrDI GCAATG_nn'
Cuts at: 0 210 281 434 933
Size: 210 71 153 499
Fragments arranged by size:
499 210 153 71
BsrGI T'GTAC_A
Cuts at: 0 611 933
Size: 611 322
BstAPI GCAn_nnn'nTGC
Cuts at: 0 778 933
Size: 778 155
BstYI r'GATC_y
Cuts at: 0 320 933
Size: 320 613
BtgI C'CryG_G
Cuts at: 0 269 933
Size: 269 664
DrdI GACnn_nn'nnGTC
Cuts at: 0 559 933
Size: 559 374
EaeI y'GGCC_r
Cuts at: 0 591 933
Size: 591 342
EarI CTCTTCn'nnn_
Cuts at: 0 127 136 835 933
Size: 127 9 699 98
Fragments arranged by size:
699 127 98 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 536 933
Size: 352 184 397
Fragments arranged by size:
397 352 184
EcoRI G'AATT_C
Cuts at: 0 310 933
Size: 310 623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 600 933
Size: 600 333
HaeII r_GCGC'y
Cuts at: 0 64 459 933
Size: 64 395 474
Fragments arranged by size:
474 395 64
HincII GTy'rAC
Cuts at: 0 347 933
Size: 347 586
KasI G'GCGC_C
Cuts at: 0 455 933
Size: 455 478
MscI TGG'CCA
Cuts at: 0 593 933
Size: 593 340
MslI CAynn'nnrTG
Cuts at: 0 758 933
Size: 758 175
NarI GG'CG_CC
Cuts at: 0 456 933
Size: 456 477
NruI TCG'CGA
Cuts at: 0 295 933
Size: 295 638
PfoI T'CCnGG_A
Cuts at: 0 32 563 933
Size: 32 531 370
Fragments arranged by size:
531 370 32
PstI C_TGCA'G
Cuts at: 0 644 692 933
Size: 644 48 241
Fragments arranged by size:
644 241 48
PvuI CG_AT'CG
Cuts at: 0 29 933
Size: 29 904
SalI G'TCGA_C
Cuts at: 0 345 933
Size: 345 588
SbfI CC_TGCA'GG
Cuts at: 0 644 933
Size: 644 289
SfcI C'TryA_G
Cuts at: 0 534 640 688 933
Size: 534 106 48 245
Fragments arranged by size:
534 245 106 48
SfoI GGC'GCC
Cuts at: 0 457 933
Size: 457 476
SmlI C'TyrA_G
Cuts at: 0 474 782 933
Size: 474 308 151
Fragments arranged by size:
474 308 151
TatI w'GTAC_w
Cuts at: 0 611 933
Size: 611 322
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 779 811 933
Size: 779 32 122
Fragments arranged by size:
779 122 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 933
Size: 765 168
XmnI GAAnn'nnTTC
Cuts at: 0 580 933
Size: 580 353
Enzymes that do cut:
AccI AcuI AlfI ApoI AvaI BanI BanII BbeI
BclI BglI BmrI BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrGI BstAPI BstYI BtgI DrdI EaeI EarI
Eco57MI EcoRI FalI HaeII HincII KasI MscI MslI
NarI NruI PfoI PstI PvuI SalI SbfI SfcI
SfoI SmlI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BsaI BsaBI BseYI BsgI BsiWI BsmI BsmBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI