Restriction map for
pF1KB4992
(Linear) (Six-base) MAPSORT of: KIBB4992.seq Check: 1081 from: 1 to: 843
KIBB4992.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 484 843
Size: 484 359
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 843
Size: 663 180
AleI CACnn'nnGTG
Cuts at: 0 25 843
Size: 25 818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 147 843
Size: 147 696
ApaLI G'TGCA_C
Cuts at: 0 166 843
Size: 166 677
ApoI r'AATT_y
Cuts at: 0 298 337 602 843
Size: 298 39 265 241
Fragments arranged by size:
298 265 241 39
BanII G_rGCy'C
Cuts at: 0 704 843
Size: 704 139
BbsI GAAGACnn'nnnn_
Cuts at: 0 833 843
Size: 833 10
BclI T'GATC_A
Cuts at: 0 426 843
Size: 426 417
BglII A'GATC_T
Cuts at: 0 431 843
Size: 431 412
BlpI GC'TnA_GC
Cuts at: 0 523 843
Size: 523 320
Bme1580I G_kGCm'C
Cuts at: 0 170 843
Size: 170 673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 843
Size: 676 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 843
Size: 192 651
BsaHI Gr'CG_yC
Cuts at: 0 481 843
Size: 481 362
BsaWI w'CCGG_w
Cuts at: 0 551 843
Size: 551 292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 843
Size: 67 776
BsiHKAI G_wGCw'C
Cuts at: 0 170 704 843
Size: 170 534 139
Fragments arranged by size:
534 170 139
BsmI GAATG_Cn'
Cuts at: 0 545 843
Size: 545 298
Bsp1286I G_dGCh'C
Cuts at: 0 170 704 843
Size: 170 534 139
Fragments arranged by size:
534 170 139
BspEI T'CCGG_A
Cuts at: 0 551 843
Size: 551 292
BsrDI GCAATG_nn'
Cuts at: 0 534 843
Size: 534 309
BsrFI r'CCGG_y
Cuts at: 0 518 843
Size: 518 325
BstYI r'GATC_y
Cuts at: 0 431 843
Size: 431 412
Bsu36I CC'TnA_GG
Cuts at: 0 794 843
Size: 794 49
EaeI y'GGCC_r
Cuts at: 0 2 516 582 843
Size: 2 514 66 261
Fragments arranged by size:
514 261 66 2
EarI CTCTTCn'nnn_
Cuts at: 0 157 806 843
Size: 157 649 37
Fragments arranged by size:
649 157 37
EcoICRI GAG'CTC
Cuts at: 0 702 843
Size: 702 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 663 676 843
Size: 663 13 167
Fragments arranged by size:
663 167 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 419 843
Size: 419 424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 843
Size: 357 486
HaeII r_GCGC'y
Cuts at: 0 409 843
Size: 409 434
HincII GTy'rAC
Cuts at: 0 72 261 843
Size: 72 189 582
Fragments arranged by size:
582 189 72
MslI CAynn'nnrTG
Cuts at: 0 25 145 722 843
Size: 25 120 577 121
Fragments arranged by size:
577 121 120 25
MspA1I CmG'CkG
Cuts at: 0 624 843
Size: 624 219
NaeI GCC'GGC
Cuts at: 0 520 843
Size: 520 323
NgoMIV G'CCGG_C
Cuts at: 0 518 843
Size: 518 325
NspI r_CATG'y
Cuts at: 0 150 507 843
Size: 150 357 336
Fragments arranged by size:
357 336 150
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 624 843
Size: 141 483 219
Fragments arranged by size:
483 219 141
PvuII CAG'CTG
Cuts at: 0 624 843
Size: 624 219
SacI G_AGCT'C
Cuts at: 0 704 843
Size: 704 139
SapI GCTCTTCn'nnn_
Cuts at: 0 157 806 843
Size: 157 649 37
Fragments arranged by size:
649 157 37
SfcI C'TryA_G
Cuts at: 0 275 843
Size: 275 568
SmlI C'TyrA_G
Cuts at: 0 171 843
Size: 171 672
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 244 843
Size: 244 599
TatI w'GTAC_w
Cuts at: 0 14 843
Size: 14 829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 453 485 843
Size: 453 32 358
Fragments arranged by size:
453 358 32
ZraI GAC'GTC
Cuts at: 0 482 843
Size: 482 361
Enzymes that do cut:
AatII AcuI AleI AlfI ApaLI ApoI BanII BbsI
BclI BglII BlpI Bme1580I BpmI BpuEI BsaHI BsaWI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrFI BstYI
Bsu36I EaeI EarI EcoICRI Eco57MI EcoNI FalI HaeII
HincII MslI MspA1I NaeI NgoMIV NspI PflMI PvuII
SacI SapI SfcI SmlI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiWI BsmBI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI