Restriction map for
pF1KB4995
(Linear) (Six-base) MAPSORT of: KIBB4995.seq Check: 108 from: 1 to: 2022
KIBB4995.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 639 2022
Size: 639 1383
AccI GT'mk_AC
Cuts at: 0 50 2022
Size: 50 1972
AfeI AGC'GCT
Cuts at: 0 343 2022
Size: 343 1679
AgeI A'CCGG_T
Cuts at: 0 1352 2022
Size: 1352 670
AleI CACnn'nnGTG
Cuts at: 0 1328 2022
Size: 1328 694
AlwNI CAG_nnn'CTG
Cuts at: 0 120 396 1027 1490 1699 2022
Size: 120 276 631 463 209 323
Fragments arranged by size:
631 463 323 276 209 120
ApaI G_GGCC'C
Cuts at: 0 1394 2022
Size: 1394 628
ApaLI G'TGCA_C
Cuts at: 0 148 2022
Size: 148 1874
AvaI C'yCGr_G
Cuts at: 0 786 957 1098 1158 1340 2022
Size: 786 171 141 60 182 682
Fragments arranged by size:
786 682 182 171 141 60
BanI G'GyrC_C
Cuts at: 0 1460 2022
Size: 1460 562
BanII G_rGCy'C
Cuts at: 0 187 1339 1394 1594 2022
Size: 187 1152 55 200 428
Fragments arranged by size:
1152 428 200 187 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1841 2022
Size: 1841 181
BbvCI CC'TCA_GC
Cuts at: 0 835 1498 2022
Size: 835 663 524
Fragments arranged by size:
835 663 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 29 30 64 229 263 2022
Size: 29 1 34 165 34 1759
Fragments arranged by size:
1759 165 34 34 29 1
BglI GCCn_nnn'nGGC
Cuts at: 0 1374 2022
Size: 1374 648
BglII A'GATC_T
Cuts at: 0 404 657 2022
Size: 404 253 1365
Fragments arranged by size:
1365 404 253
Bme1580I G_kGCm'C
Cuts at: 0 152 252 1394 2022
Size: 152 100 1142 628
Fragments arranged by size:
1142 628 152 100
BmgBI CAC'GTC
Cuts at: 0 615 627 2022
Size: 615 12 1395
Fragments arranged by size:
1395 615 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 108 2022
Size: 108 1914
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 939 951 2022
Size: 597 342 12 1071
Fragments arranged by size:
1071 597 342 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1152 1164 1176 2022
Size: 418 734 12 12 846
Fragments arranged by size:
846 734 418 12 12
Bpu10I CC'TnA_GC
Cuts at: 0 835 1498 2022
Size: 835 663 524
Fragments arranged by size:
835 663 524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1971 2022
Size: 784 1187 51
Fragments arranged by size:
1187 784 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 432 2022
Size: 432 1590
BsaHI Gr'CG_yC
Cuts at: 0 792 804 1717 2022
Size: 792 12 913 305
Fragments arranged by size:
913 792 305 12
BsaWI w'CCGG_w
Cuts at: 0 716 1352 2022
Size: 716 636 670
Fragments arranged by size:
716 670 636
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 1231 1261 1342 1372 2022
Size: 541 30 660 30 81 30 650
Fragments arranged by size:
660 650 541 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 562 565 568 583 586 745 781
Size: 400 162 3 3 15 3 159 36
Cuts at: 781 784 982 1024 1119 1122 1125 1128 1131
Size: 3 198 42 95 3 3 3 3
Cuts at: 1131 1134 1137 2022
Size: 3 3 885
Fragments arranged by size:
885 400 198 162 159 95 42 36
15 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 108 442 859 1079 1546 1552 2022
Size: 108 334 417 220 467 6 470
Fragments arranged by size:
470 467 417 334 220 108 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 2022
Size: 379 1643
BsiEI CG_ry'CG
Cuts at: 0 566 1236 1449 1634 2022
Size: 566 670 213 185 388
Fragments arranged by size:
670 566 388 213 185
BsiHKAI G_wGCw'C
Cuts at: 0 152 2022
Size: 152 1870
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 1337 2022
Size: 69 1268 685
Fragments arranged by size:
1268 685 69
Bsp1286I G_dGCh'C
Cuts at: 0 152 187 252 1339 1394 1594 2022
Size: 152 35 65 1087 55 200 428
Fragments arranged by size:
1087 428 200 152 65 55 35
BspEI T'CCGG_A
Cuts at: 0 716 2022
Size: 716 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 114 639 1683 2022
Size: 114 525 1044 339
Fragments arranged by size:
1044 525 339 114
BsrBI CCG'CTC
Cuts at: 0 27 72 797 935 941 947 1127 1178
Size: 27 45 725 138 6 6 180 51
Cuts at: 1178 1202 1247 1259 1271 1277 1283 1364 1415
Size: 24 45 12 12 6 6 81 51
Cuts at: 1415 1821 1830 1878 1937 2022
Size: 406 9 48 59 85
Fragments arranged by size:
725 406 180 138 85 81 59 51
51 48 45 45 27 24 12 12
9 6 6 6 6
BsrFI r'CCGG_y
Cuts at: 0 373 1316 1352 1629 1925 2022
Size: 373 943 36 277 296 97
Fragments arranged by size:
943 373 296 277 97 36
BssSI C'ACGA_G
Cuts at: 0 7 2022
Size: 7 2015
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1027 2022
Size: 1027 995
BstEII G'GTnAC_C
Cuts at: 0 905 2022
Size: 905 1117
BstYI r'GATC_y
Cuts at: 0 404 657 1862 2022
Size: 404 253 1205 160
Fragments arranged by size:
1205 404 253 160
Bsu36I CC'TnA_GG
Cuts at: 0 1456 1721 2022
Size: 1456 265 301
Fragments arranged by size:
1456 301 265
BtgI C'CryG_G
Cuts at: 0 1209 1311 1850 2022
Size: 1209 102 539 172
Fragments arranged by size:
1209 539 172 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1019 2022
Size: 1019 1003
BtsI GCAGTG_nn'
Cuts at: 0 397 2022
Size: 397 1625
EaeI y'GGCC_r
Cuts at: 0 132 173 515 563 689 1233 1314 1446
Size: 132 41 342 48 126 544 81 132
Cuts at: 1446 1631 2022
Size: 185 391
Fragments arranged by size:
544 391 342 185 132 132 126 81
48 41
EagI C'GGCC_G
Cuts at: 0 563 1233 1446 1631 2022
Size: 563 670 213 185 391
Fragments arranged by size:
670 563 391 213 185
EarI CTCTTCn'nnn_
Cuts at: 0 55 1155 1898 1977 2022
Size: 55 1100 743 79 45
Fragments arranged by size:
1100 743 79 55 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 1789 2022
Size: 82 1707 233
Fragments arranged by size:
1707 233 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1152 1164 1176 2022
Size: 418 734 12 12 846
Fragments arranged by size:
846 734 418 12 12
EcoO109I rG'GnC_Cy
Cuts at: 0 105 1163 1391 1452 1991 2022
Size: 105 1058 228 61 539 31
Fragments arranged by size:
1058 539 228 105 61 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1858 2022
Size: 1858 164
FseI GG_CCGG'CC
Cuts at: 0 1633 2022
Size: 1633 389
HaeII r_GCGC'y
Cuts at: 0 345 1118 1293 1756 1986 2022
Size: 345 773 175 463 230 36
Fragments arranged by size:
773 463 345 230 175 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 541 573 2022
Size: 227 32 282 32 1449
Fragments arranged by size:
1449 282 227 32 32
HincII GTy'rAC
Cuts at: 0 51 2022
Size: 51 1971
HindIII A'AGCT_T
Cuts at: 0 31 2022
Size: 31 1991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 2022
Size: 296 1726
MslI CAynn'nnrTG
Cuts at: 0 613 1328 2022
Size: 613 715 694
Fragments arranged by size:
715 694 613
MspA1I CmG'CkG
Cuts at: 0 178 562 810 1027 1490 1681 2022
Size: 178 384 248 217 463 191 341
Fragments arranged by size:
463 384 341 248 217 191 178
NaeI GCC'GGC
Cuts at: 0 375 1318 1631 1927 2022
Size: 375 943 313 296 95
Fragments arranged by size:
943 375 313 296 95
NcoI C'CATG_G
Cuts at: 0 1850 2022
Size: 1850 172
NgoMIV G'CCGG_C
Cuts at: 0 373 1316 1629 1925 2022
Size: 373 943 313 296 97
Fragments arranged by size:
943 373 313 296 97
NspI r_CATG'y
Cuts at: 0 754 2022
Size: 754 1268
PpuMI rG'GwC_Cy
Cuts at: 0 105 1163 1991 2022
Size: 105 1058 828 31
Fragments arranged by size:
1058 828 105 31
PspOMI G'GGCC_C
Cuts at: 0 1390 2022
Size: 1390 632
PspXI vC'TCGA_Gb
Cuts at: 0 1158 2022
Size: 1158 864
PstI C_TGCA'G
Cuts at: 0 125 165 2022
Size: 125 40 1857
Fragments arranged by size:
1857 125 40
PvuII CAG'CTG
Cuts at: 0 1490 2022
Size: 1490 532
SalI G'TCGA_C
Cuts at: 0 49 2022
Size: 49 1973
SapI GCTCTTCn'nnn_
Cuts at: 0 1977 2022
Size: 1977 45
SbfI CC_TGCA'GG
Cuts at: 0 125 2022
Size: 125 1897
SfcI C'TryA_G
Cuts at: 0 121 161 345 1434 2022
Size: 121 40 184 1089 588
Fragments arranged by size:
1089 588 184 121 40
SmaI CCC'GGG
Cuts at: 0 959 1100 2022
Size: 959 141 922
Fragments arranged by size:
959 922 141
SmlI C'TyrA_G
Cuts at: 0 763 1158 1986 2022
Size: 763 395 828 36
Fragments arranged by size:
828 763 395 36
SphI G_CATG'C
Cuts at: 0 754 2022
Size: 754 1268
StuI AGG'CCT
Cuts at: 0 353 431 2022
Size: 353 78 1591
Fragments arranged by size:
1591 353 78
StyI C'CwwG_G
Cuts at: 0 755 1850 2022
Size: 755 1095 172
Fragments arranged by size:
1095 755 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 248 873 1684 2022
Size: 248 625 811 338
Fragments arranged by size:
811 625 338 248
TatI w'GTAC_w
Cuts at: 0 782 1919 2022
Size: 782 1137 103
Fragments arranged by size:
1137 782 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1342 1374 2022
Size: 1231 32 79 32 648
Fragments arranged by size:
1231 648 79 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 703 2022
Size: 703 1319
XhoI C'TCGA_G
Cuts at: 0 1158 2022
Size: 1158 864
XmaI C'CCGG_G
Cuts at: 0 957 1098 2022
Size: 957 141 924
Fragments arranged by size:
957 924 141
Enzymes that do cut:
AarI AccI AfeI AgeI AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbsI BbvCI BcgI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI Eco57MI EcoO109I FalI FseI HaeII Hin4I
HincII HindIII MmeI MslI MspA1I NaeI NcoI NgoMIV
NspI PpuMI PspOMI PspXI PstI PvuII SalI SapI
SbfI SfcI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BclI BfrBI BlpI BmtI BsaAI BsaBI
BsiWI BsmI BspHI BsrDI BsrGI BssHII BstBI BstXI
BstZ17I ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoRI EcoRV FspI FspAI HpaI KasI KpnI MfeI
MluI MscI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SacII SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XcmI XmnI ZraI