(Linear) (Six-base) MAPSORT of: KIBB5000.seq  Check: 1417  from: 1  to: 612



KIBB5000.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     612 
   Size:        277     335
 
ApoI r'AATT_y

Cuts at:      0     348     382     612 
   Size:        348      34     230

  Fragments arranged by size: 

                 348     230      34
 
BanII G_rGCy'C

Cuts at:      0     117     336     612 
   Size:        117     219     276

  Fragments arranged by size: 

                 276     219     117
 
BglI GCCn_nnn'nGGC

Cuts at:      0     152     206     612 
   Size:        152      54     406

  Fragments arranged by size: 

                 406     152      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     160     214     366     438     612 
   Size:        160      54     152      72     174

  Fragments arranged by size: 

                 174     160     152      72      54
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     612 
   Size:        577      35
 
BsaHI Gr'CG_yC

Cuts at:      0     321     612 
   Size:        321     291
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     322     612 
   Size:        322     290
 
Bsp1286I G_dGCh'C

Cuts at:      0     117     336     612 
   Size:        117     219     276

  Fragments arranged by size: 

                 276     219     117
 
BssHII G'CGCG_C

Cuts at:      0     142     612 
   Size:        142     470
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     550     612 
   Size:        550      62
 
Bsu36I CC'TnA_GG

Cuts at:      0     227     612 
   Size:        227     385
 
BtgI C'CryG_G

Cuts at:      0     201     513     612 
   Size:        201     312      99

  Fragments arranged by size: 

                 312     201      99
 
DraIII CAC_nnn'GTG

Cuts at:      0     446     612 
   Size:        446     166
 
EaeI y'GGCC_r

Cuts at:      0     207     612 
   Size:        207     405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     577     612 
   Size:        277     300      35

  Fragments arranged by size: 

                 300     277      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     523     612 
   Size:        523      89
 
EcoRI G'AATT_C

Cuts at:      0     348     612 
   Size:        348     264
 
HincII GTy'rAC

Cuts at:      0     267     612 
   Size:        267     345
 
HpaI GTT'AAC

Cuts at:      0     267     612 
   Size:        267     345
 
MslI CAynn'nnrTG

Cuts at:      0     260     612 
   Size:        260     352
 
MspA1I CmG'CkG

Cuts at:      0      77     612 
   Size:         77     535
 
PfoI T'CCnGG_A

Cuts at:      0      20     612 
   Size:         20     592
 
PstI C_TGCA'G

Cuts at:      0     501     612 
   Size:        501     111
 
SfcI C'TryA_G

Cuts at:      0     497     612 
   Size:        497     115
 
StyI C'CwwG_G

Cuts at:      0     519     612 
   Size:        519      93
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     457     612 
   Size:        457     155
 
TatI w'GTAC_w

Cuts at:      0      14     612 
   Size:         14     598
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     547     550     612 
   Size:        547       3      62

  Fragments arranged by size: 

                 547      62       3
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BglI     BmrI     BpmI    BsaHI    BseRI
 Bsp1286I   BssHII    BstXI   Bsu36I     BtgI   DraIII     EaeI  Eco57MI
    EcoNI    EcoRI   HincII     HpaI     MslI   MspA1I     PfoI     PstI
     SfcI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI