Restriction map for
pF1KB5005
(Linear) (Six-base) MAPSORT of: KIBB5005.seq Check: 3167 from: 1 to: 1254
KIBB5005.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 889 1254
Size: 138 751 365
Fragments arranged by size:
751 365 138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 523 1254
Size: 523 731
AlwNI CAG_nnn'CTG
Cuts at: 0 88 1254
Size: 88 1166
ApaLI G'TGCA_C
Cuts at: 0 319 1254
Size: 319 935
ApoI r'AATT_y
Cuts at: 0 637 1254
Size: 637 617
AvaI C'yCGr_G
Cuts at: 0 556 832 1254
Size: 556 276 422
Fragments arranged by size:
556 422 276
BanI G'GyrC_C
Cuts at: 0 589 1211 1254
Size: 589 622 43
Fragments arranged by size:
622 589 43
BanII G_rGCy'C
Cuts at: 0 112 239 1254
Size: 112 127 1015
Fragments arranged by size:
1015 127 112
BbeI G_GCGC'C
Cuts at: 0 593 1215 1254
Size: 593 622 39
Fragments arranged by size:
622 593 39
BglI GCCn_nnn'nGGC
Cuts at: 0 148 1254
Size: 148 1106
BglII A'GATC_T
Cuts at: 0 872 1254
Size: 872 382
BlpI GC'TnA_GC
Cuts at: 0 289 1254
Size: 289 965
Bme1580I G_kGCm'C
Cuts at: 0 323 1254
Size: 323 931
BmgBI CAC'GTC
Cuts at: 0 573 1254
Size: 573 681
BmrI ACTGGGnnnn_n'
Cuts at: 0 636 1254
Size: 636 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 777 1254
Size: 58 719 477
Fragments arranged by size:
719 477 58
BsaAI yAC'GTr
Cuts at: 0 822 1254
Size: 822 432
BsaHI Gr'CG_yC
Cuts at: 0 268 590 1212 1254
Size: 268 322 622 42
Fragments arranged by size:
622 322 268 42
BsaWI w'CCGG_w
Cuts at: 0 713 1254
Size: 713 541
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 775 805 1254
Size: 775 30 449
Fragments arranged by size:
775 449 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 1135 1254
Size: 328 807 119
Fragments arranged by size:
807 328 119
BseYI C'CCAG_C
Cuts at: 0 231 246 387 409 964 969 1254
Size: 231 15 141 22 555 5 285
Fragments arranged by size:
555 285 231 141 22 15 5
BsiHKAI G_wGCw'C
Cuts at: 0 323 473 1254
Size: 323 150 781
Fragments arranged by size:
781 323 150
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1238 1254
Size: 1238 16
Bsp1286I G_dGCh'C
Cuts at: 0 112 239 323 473 1254
Size: 112 127 84 150 781
Fragments arranged by size:
781 150 127 112 84
BspHI T'CATG_A
Cuts at: 0 701 1254
Size: 701 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 956 1254
Size: 78 878 298
Fragments arranged by size:
878 298 78
BsrFI r'CCGG_y
Cuts at: 0 142 325 1254
Size: 142 183 929
Fragments arranged by size:
929 183 142
BssHII G'CGCG_C
Cuts at: 0 367 503 1254
Size: 367 136 751
Fragments arranged by size:
751 367 136
BssSI C'ACGA_G
Cuts at: 0 238 1254
Size: 238 1016
BstEII G'GTnAC_C
Cuts at: 0 561 936 1254
Size: 561 375 318
Fragments arranged by size:
561 375 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 927 1254
Size: 573 354 327
Fragments arranged by size:
573 354 327
BstYI r'GATC_y
Cuts at: 0 872 1254
Size: 872 382
Bsu36I CC'TnA_GG
Cuts at: 0 1226 1254
Size: 1226 28
BtgI C'CryG_G
Cuts at: 0 170 212 263 362 663 692 1254
Size: 170 42 51 99 301 29 562
Fragments arranged by size:
562 301 170 99 51 42 29
BtsI GCAGTG_nn'
Cuts at: 0 92 194 290 1254
Size: 92 102 96 964
Fragments arranged by size:
964 102 96 92
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1050 1085 1254
Size: 1050 35 169
Fragments arranged by size:
1050 169 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 267 1254
Size: 267 987
EaeI y'GGCC_r
Cuts at: 0 50 119 173 1043 1254
Size: 50 69 54 870 211
Fragments arranged by size:
870 211 69 54 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 138 777 889 1254
Size: 58 80 639 112 365
Fragments arranged by size:
639 365 112 80 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 979 1254
Size: 37 942 275
Fragments arranged by size:
942 275 37
EcoO109I rG'GnC_Cy
Cuts at: 0 1032 1254
Size: 1032 222
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 501 1023 1254
Size: 501 522 231
Fragments arranged by size:
522 501 231
FspI TGC'GCA
Cuts at: 0 442 1129 1254
Size: 442 687 125
Fragments arranged by size:
687 442 125
HaeII r_GCGC'y
Cuts at: 0 26 143 296 593 840 1215 1254
Size: 26 117 153 297 247 375 39
Fragments arranged by size:
375 297 247 153 117 39 26
HincII GTy'rAC
Cuts at: 0 603 1254
Size: 603 651
KasI G'GCGC_C
Cuts at: 0 589 1211 1254
Size: 589 622 43
Fragments arranged by size:
622 589 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1254
Size: 1055 199
MscI TGG'CCA
Cuts at: 0 175 1045 1254
Size: 175 870 209
Fragments arranged by size:
870 209 175
MslI CAynn'nnrTG
Cuts at: 0 202 629 706 808 1254
Size: 202 427 77 102 446
Fragments arranged by size:
446 427 202 102 77
MspA1I CmG'CkG
Cuts at: 0 303 867 1254
Size: 303 564 387
Fragments arranged by size:
564 387 303
NaeI GCC'GGC
Cuts at: 0 144 327 1254
Size: 144 183 927
Fragments arranged by size:
927 183 144
NarI GG'CG_CC
Cuts at: 0 590 1212 1254
Size: 590 622 42
Fragments arranged by size:
622 590 42
NcoI C'CATG_G
Cuts at: 0 170 1254
Size: 170 1084
NgoMIV G'CCGG_C
Cuts at: 0 142 325 1254
Size: 142 183 929
Fragments arranged by size:
929 183 142
NspI r_CATG'y
Cuts at: 0 1020 1254
Size: 1020 234
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 189 651 822 1254
Size: 171 18 462 171 432
Fragments arranged by size:
462 432 171 171 18
PfoI T'CCnGG_A
Cuts at: 0 35 1254
Size: 35 1219
PmlI CAC'GTG
Cuts at: 0 822 1254
Size: 822 432
PpuMI rG'GwC_Cy
Cuts at: 0 1032 1254
Size: 1032 222
PstI C_TGCA'G
Cuts at: 0 75 512 953 1254
Size: 75 437 441 301
Fragments arranged by size:
441 437 301 75
PvuII CAG'CTG
Cuts at: 0 303 867 1254
Size: 303 564 387
Fragments arranged by size:
564 387 303
SfcI C'TryA_G
Cuts at: 0 71 508 949 1254
Size: 71 437 441 305
Fragments arranged by size:
441 437 305 71
SfoI GGC'GCC
Cuts at: 0 591 1213 1254
Size: 591 622 41
Fragments arranged by size:
622 591 41
SmlI C'TyrA_G
Cuts at: 0 832 1254
Size: 832 422
StuI AGG'CCT
Cuts at: 0 722 1254
Size: 722 532
StyI C'CwwG_G
Cuts at: 0 113 170 176 278 566 920 1254
Size: 113 57 6 102 288 354 334
Fragments arranged by size:
354 334 288 113 102 57 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 671 685 1198 1254
Size: 671 14 513 56
Fragments arranged by size:
671 513 56 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 775 807 1254
Size: 775 32 447
Fragments arranged by size:
775 447 32
Tth111I GACn'n_nGTC
Cuts at: 0 681 1254
Size: 681 573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 927 1254
Size: 927 327
XhoI C'TCGA_G
Cuts at: 0 832 1254
Size: 832 422
Enzymes that do cut:
AcuI AlfI AlwNI ApaLI ApoI AvaI BanI BanII
BbeI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BssHII BssSI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI Eco57MI
EcoNI EcoO109I FalI FspI HaeII HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PfoI PmlI PpuMI PstI PvuII SfcI SfoI
SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsgI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BstAPI
BstBI BstZ17I BtgZI ClaI DraI DraIII EagI EarI
EciI EcoICRI EcoRI EcoRV FseI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XmaI XmnI ZraI