Restriction map for
pF1KB5008
(Linear) (Six-base) MAPSORT of: KIBB5008.seq Check: 5367 from: 1 to: 882
KIBB5008.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 12 559 882
Size: 12 547 323
Fragments arranged by size:
547 323 12
AleI CACnn'nnGTG
Cuts at: 0 772 882
Size: 772 110
AlwNI CAG_nnn'CTG
Cuts at: 0 738 882
Size: 738 144
ApaI G_GGCC'C
Cuts at: 0 108 824 882
Size: 108 716 58
Fragments arranged by size:
716 108 58
AvaI C'yCGr_G
Cuts at: 0 770 882
Size: 770 112
BanI G'GyrC_C
Cuts at: 0 540 826 882
Size: 540 286 56
Fragments arranged by size:
540 286 56
BanII G_rGCy'C
Cuts at: 0 108 320 815 824 882
Size: 108 212 495 9 58
Fragments arranged by size:
495 212 108 58 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 496 530 882
Size: 496 34 352
Fragments arranged by size:
496 352 34
BclI T'GATC_A
Cuts at: 0 668 882
Size: 668 214
BglI GCCn_nnn'nGGC
Cuts at: 0 178 882
Size: 178 704
Bme1580I G_kGCm'C
Cuts at: 0 108 519 545 824 882
Size: 108 411 26 279 58
Fragments arranged by size:
411 279 108 58 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 726 882
Size: 726 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 882
Size: 167 715
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 882
Size: 43 839
BsaI GGTCTCn'nnnn_
Cuts at: 0 231 882
Size: 231 651
BsaAI yAC'GTr
Cuts at: 0 645 882
Size: 645 237
BsaHI Gr'CG_yC
Cuts at: 0 209 247 882
Size: 209 38 635
Fragments arranged by size:
635 209 38
BsaWI w'CCGG_w
Cuts at: 0 347 882
Size: 347 535
BseYI C'CCAG_C
Cuts at: 0 835 882
Size: 835 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 649 882
Size: 639 10 233
Fragments arranged by size:
639 233 10
BsiHKAI G_wGCw'C
Cuts at: 0 320 797 882
Size: 320 477 85
Fragments arranged by size:
477 320 85
BsmI GAATG_Cn'
Cuts at: 0 280 882
Size: 280 602
Bsp1286I G_dGCh'C
Cuts at: 0 108 320 519 545 797 815 824 882
Size: 108 212 199 26 252 18 9 58
Fragments arranged by size:
252 212 199 108 58 26 18 9
BspEI T'CCGG_A
Cuts at: 0 347 882
Size: 347 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 559 882
Size: 12 547 323
Fragments arranged by size:
547 323 12
BsrBI CCG'CTC
Cuts at: 0 144 882
Size: 144 738
BsrGI T'GTAC_A
Cuts at: 0 287 761 882
Size: 287 474 121
Fragments arranged by size:
474 287 121
BssHII G'CGCG_C
Cuts at: 0 35 37 882
Size: 35 2 845
Fragments arranged by size:
845 35 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 860 882
Size: 860 22
BstBI TT'CG_AA
Cuts at: 0 384 882
Size: 384 498
BstYI r'GATC_y
Cuts at: 0 299 882
Size: 299 583
BtsI GCAGTG_nn'
Cuts at: 0 519 882
Size: 519 363
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 444 479 882
Size: 444 35 403
Fragments arranged by size:
444 403 35
DraIII CAC_nnn'GTG
Cuts at: 0 590 882
Size: 590 292
EarI CTCTTCn'nnn_
Cuts at: 0 381 882
Size: 381 501
EcoICRI GAG'CTC
Cuts at: 0 318 882
Size: 318 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 882
Size: 167 715
EcoO109I rG'GnC_Cy
Cuts at: 0 104 105 820 882
Size: 104 1 715 62
Fragments arranged by size:
715 104 62 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 512 882
Size: 480 32 370
Fragments arranged by size:
480 370 32
HincII GTy'rAC
Cuts at: 0 582 882
Size: 582 300
MslI CAynn'nnrTG
Cuts at: 0 220 238 643 697 772 787 882
Size: 220 18 405 54 75 15 95
Fragments arranged by size:
405 220 95 75 54 18 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 882
Size: 738 144
PspOMI G'GGCC_C
Cuts at: 0 104 820 882
Size: 104 716 62
Fragments arranged by size:
716 104 62
PstI C_TGCA'G
Cuts at: 0 514 882
Size: 514 368
SacI G_AGCT'C
Cuts at: 0 320 882
Size: 320 562
SfcI C'TryA_G
Cuts at: 0 510 882
Size: 510 372
SmaI CCC'GGG
Cuts at: 0 772 882
Size: 772 110
SmlI C'TyrA_G
Cuts at: 0 58 882
Size: 58 824
StyI C'CwwG_G
Cuts at: 0 294 882
Size: 294 588
TatI w'GTAC_w
Cuts at: 0 287 761 882
Size: 287 474 121
Fragments arranged by size:
474 287 121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 837 882
Size: 837 45
XmaI C'CCGG_G
Cuts at: 0 770 882
Size: 770 112
XmnI GAAnn'nnTTC
Cuts at: 0 451 882
Size: 451 431
Enzymes that do cut:
AarI AleI AlwNI ApaI AvaI BanI BanII BcgI
BclI BglI Bme1580I BmrI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrGI BssHII BstAPI BstBI BstYI BtsI
CspCI DraIII EarI EcoICRI Eco57MI EcoO109I Hin4I HincII
MslI PflMI PspOMI PstI SacI SfcI SmaI SmlI
StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaBI
BsaXI BseRI BsiEI BsiWI BsmBI BspHI BsrDI BsrFI
BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI ZraI