Restriction map for
pF1KB5012
(Linear) (Six-base) MAPSORT of: KIBB5012.seq Check: 6033 from: 1 to: 1629
KIBB5012.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 194 803 1629
Size: 194 609 826
Fragments arranged by size:
826 609 194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1057 1629
Size: 40 1017 572
Fragments arranged by size:
1017 572 40
AfeI AGC'GCT
Cuts at: 0 1408 1629
Size: 1408 221
AlwNI CAG_nnn'CTG
Cuts at: 0 675 1599 1629
Size: 675 924 30
Fragments arranged by size:
924 675 30
ApoI r'AATT_y
Cuts at: 0 367 1629
Size: 367 1262
BamHI G'GATC_C
Cuts at: 0 99 1629
Size: 99 1530
BbsI GAAGACnn'nnnn_
Cuts at: 0 887 1629
Size: 887 742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 174 208 1187 1221 1629
Size: 174 34 979 34 408
Fragments arranged by size:
979 408 174 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1270 1629
Size: 1270 359
BglI GCCn_nnn'nGGC
Cuts at: 0 355 1629
Size: 355 1274
BglII A'GATC_T
Cuts at: 0 648 1629
Size: 648 981
Bme1580I G_kGCm'C
Cuts at: 0 502 1629
Size: 502 1127
BmrI ACTGGGnnnn_n'
Cuts at: 0 219 1629
Size: 219 1410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1243 1288 1359 1629
Size: 1243 45 71 270
Fragments arranged by size:
1243 270 71 45
Bpu10I CC'TnA_GC
Cuts at: 0 43 1629
Size: 43 1586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1225 1629
Size: 991 234 404
Fragments arranged by size:
991 404 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 616 1629
Size: 616 1013
BsaAI yAC'GTr
Cuts at: 0 114 1629
Size: 114 1515
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 337 367 1357 1387 1629
Size: 337 30 990 30 242
Fragments arranged by size:
990 337 242 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 589 1099 1629
Size: 301 288 510 530
Fragments arranged by size:
530 510 301 288
BseYI C'CCAG_C
Cuts at: 0 690 1503 1629
Size: 690 813 126
Fragments arranged by size:
813 690 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1504 1629
Size: 1504 125
BsiHKAI G_wGCw'C
Cuts at: 0 989 1007 1629
Size: 989 18 622
Fragments arranged by size:
989 622 18
Bsp1286I G_dGCh'C
Cuts at: 0 502 989 1007 1629
Size: 502 487 18 622
Fragments arranged by size:
622 502 487 18
BspHI T'CATG_A
Cuts at: 0 371 524 1493 1629
Size: 371 153 969 136
Fragments arranged by size:
969 371 153 136
BsrBI CCG'CTC
Cuts at: 0 1050 1629
Size: 1050 579
BsrFI r'CCGG_y
Cuts at: 0 1430 1629
Size: 1430 199
BsrGI T'GTAC_A
Cuts at: 0 119 1629
Size: 119 1510
BssSI C'ACGA_G
Cuts at: 0 301 1629
Size: 301 1328
BstXI CCAn_nnnn'nTGG
Cuts at: 0 759 861 1221 1341 1629
Size: 759 102 360 120 288
Fragments arranged by size:
759 360 288 120 102
BstYI r'GATC_y
Cuts at: 0 99 513 648 1589 1629
Size: 99 414 135 941 40
Fragments arranged by size:
941 414 135 99 40
BstZ17I GTA'TAC
Cuts at: 0 804 1629
Size: 804 825
Bsu36I CC'TnA_GG
Cuts at: 0 1478 1629
Size: 1478 151
BtgI C'CryG_G
Cuts at: 0 1340 1466 1629
Size: 1340 126 163
Fragments arranged by size:
1340 163 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1333 1629
Size: 1333 296
ClaI AT'CG_AT
Cuts at: 0 59 1629
Size: 59 1570
EaeI y'GGCC_r
Cuts at: 0 1469 1629
Size: 1469 160
EarI CTCTTCn'nnn_
Cuts at: 0 575 1629
Size: 575 1054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1057 1243 1288 1359 1629
Size: 40 1017 186 45 71 270
Fragments arranged by size:
1017 270 186 71 45 40
EcoO109I rG'GnC_Cy
Cuts at: 0 39 1539 1629
Size: 39 1500 90
Fragments arranged by size:
1500 90 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 667 735 1629
Size: 667 68 894
Fragments arranged by size:
894 667 68
HaeII r_GCGC'y
Cuts at: 0 1363 1410 1629
Size: 1363 47 219
Fragments arranged by size:
1363 219 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1357 1389 1629
Size: 1357 32 240
Fragments arranged by size:
1357 240 32
HincII GTy'rAC
Cuts at: 0 195 1629
Size: 195 1434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 1629
Size: 389 1240
MslI CAynn'nnrTG
Cuts at: 0 772 859 1345 1629
Size: 772 87 486 284
Fragments arranged by size:
772 486 284 87
MspA1I CmG'CkG
Cuts at: 0 799 1233 1266 1306 1379 1629
Size: 799 434 33 40 73 250
Fragments arranged by size:
799 434 250 73 40 33
NcoI C'CATG_G
Cuts at: 0 1340 1466 1629
Size: 1340 126 163
Fragments arranged by size:
1340 163 126
NdeI CA'TA_TG
Cuts at: 0 906 1629
Size: 906 723
NspI r_CATG'y
Cuts at: 0 390 1629
Size: 390 1239
PfoI T'CCnGG_A
Cuts at: 0 1220 1520 1629
Size: 1220 300 109
Fragments arranged by size:
1220 300 109
PmlI CAC'GTG
Cuts at: 0 114 1629
Size: 114 1515
PpuMI rG'GwC_Cy
Cuts at: 0 1539 1629
Size: 1539 90
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 688 720 1629
Size: 688 32 909
Fragments arranged by size:
909 688 32
PvuII CAG'CTG
Cuts at: 0 1233 1266 1379 1629
Size: 1233 33 113 250
Fragments arranged by size:
1233 250 113 33
SalI G'TCGA_C
Cuts at: 0 193 1629
Size: 193 1436
SmlI C'TyrA_G
Cuts at: 0 1006 1240 1629
Size: 1006 234 389
Fragments arranged by size:
1006 389 234
StyI C'CwwG_G
Cuts at: 0 546 752 1340 1466 1629
Size: 546 206 588 126 163
Fragments arranged by size:
588 546 206 163 126
TatI w'GTAC_w
Cuts at: 0 119 944 1424 1629
Size: 119 825 480 205
Fragments arranged by size:
825 480 205 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1574 1606 1629
Size: 1574 32 23
Fragments arranged by size:
1574 32 23
XbaI T'CTAG_A
Cuts at: 0 243 1629
Size: 243 1386
XmnI GAAnn'nnTTC
Cuts at: 0 379 650 1629
Size: 379 271 979
Fragments arranged by size:
979 379 271
Enzymes that do cut:
AccI AcuI AfeI AlwNI ApoI BamHI BbsI BcgI
BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrBI BsrFI BsrGI BssSI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI EaeI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HincII MmeI MslI MspA1I NcoI
NdeI NspI PfoI PmlI PpuMI PsrI PvuII SalI
SmlI StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbvCI BclI
BfrBI BlpI BmgBI BmtI BplI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrDI BssHII
BstAPI BstBI BstEII BtsI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XcmI XhoI XmaI ZraI