Restriction map for
pF1KB5014
(Linear) (Six-base) MAPSORT of: KIBB5014.seq Check: 5483 from: 1 to: 1251
KIBB5014.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 62 1251
Size: 62 1189
AflII C'TTAA_G
Cuts at: 0 1099 1251
Size: 1099 152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 1251
Size: 478 773
AlwNI CAG_nnn'CTG
Cuts at: 0 81 270 1251
Size: 81 189 981
Fragments arranged by size:
981 189 81
ApoI r'AATT_y
Cuts at: 0 142 1251
Size: 142 1109
AvaI C'yCGr_G
Cuts at: 0 715 748 889 1251
Size: 715 33 141 362
Fragments arranged by size:
715 362 141 33
BamHI G'GATC_C
Cuts at: 0 627 866 1251
Size: 627 239 385
Fragments arranged by size:
627 385 239
BanI G'GyrC_C
Cuts at: 0 418 970 1251
Size: 418 552 281
Fragments arranged by size:
552 418 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 656 1121 1251
Size: 596 60 465 130
Fragments arranged by size:
596 465 130 60
BbvCI CC'TCA_GC
Cuts at: 0 1016 1251
Size: 1016 235
BclI T'GATC_A
Cuts at: 0 467 1251
Size: 467 784
BglI GCCn_nnn'nGGC
Cuts at: 0 35 1251
Size: 35 1216
Bme1580I G_kGCm'C
Cuts at: 0 862 1251
Size: 862 389
BmgBI CAC'GTC
Cuts at: 0 1037 1251
Size: 1037 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 663 714 765 784 902 1251
Size: 663 51 51 19 118 349
Fragments arranged by size:
663 349 118 51 51 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 1251
Size: 597 654
Bpu10I CC'TnA_GC
Cuts at: 0 1016 1251
Size: 1016 235
BsaWI w'CCGG_w
Cuts at: 0 645 1251
Size: 645 606
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 193 1033 1102 1120 1147 1150 1153
Size: 22 171 840 69 18 27 3 3
Cuts at: 1153 1198 1207 1228 1251
Size: 45 9 21 23
Fragments arranged by size:
840 171 69 45 27 23 22 21
18 9 3 3
BseYI C'CCAG_C
Cuts at: 0 939 1251
Size: 939 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1251
Size: 298 953
BsmBI CGTCTCn'nnnn_
Cuts at: 0 83 1027 1251
Size: 83 944 224
Fragments arranged by size:
944 224 83
Bsp1286I G_dGCh'C
Cuts at: 0 862 1251
Size: 862 389
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 948 1251
Size: 948 303
BsrBI CCG'CTC
Cuts at: 0 663 1251
Size: 663 588
BsrGI T'GTAC_A
Cuts at: 0 512 1251
Size: 512 739
BstYI r'GATC_y
Cuts at: 0 627 866 1251
Size: 627 239 385
Fragments arranged by size:
627 385 239
Bsu36I CC'TnA_GG
Cuts at: 0 698 1251
Size: 698 553
ClaI AT'CG_AT
Cuts at: 0 674 1251
Size: 674 577
EaeI y'GGCC_r
Cuts at: 0 41 233 529 1230 1251
Size: 41 192 296 701 21
Fragments arranged by size:
701 296 192 41 21
EarI CTCTTCn'nnn_
Cuts at: 0 758 1251
Size: 758 493
EcoNI CCTnn'n_nnAGG
Cuts at: 0 955 1251
Size: 955 296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 612 644 663 695 1251
Size: 612 32 19 32 556
Fragments arranged by size:
612 556 32 32 19
MspA1I CmG'CkG
Cuts at: 0 18 107 1251
Size: 18 89 1144
Fragments arranged by size:
1144 89 18
NspI r_CATG'y
Cuts at: 0 366 1251
Size: 366 885
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 1251
Size: 270 981
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 793 825 1251
Size: 793 32 426
Fragments arranged by size:
793 426 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 35 1251
Size: 35 1216
SmlI C'TyrA_G
Cuts at: 0 1099 1251
Size: 1099 152
StyI C'CwwG_G
Cuts at: 0 584 1238 1251
Size: 584 654 13
Fragments arranged by size:
654 584 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 627 887 1251
Size: 627 260 364
Fragments arranged by size:
627 364 260
TatI w'GTAC_w
Cuts at: 0 512 809 1251
Size: 512 297 442
Fragments arranged by size:
512 442 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 1251
Size: 763 32 456
Fragments arranged by size:
763 456 32
Enzymes that do cut:
AccI AflII AlfI AlwNI ApoI AvaI BamHI BanI
BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI BplI
Bpu10I BsaWI BseRI BseYI BsgI BsmBI Bsp1286I BspMI
BsrBI BsrGI BstYI Bsu36I ClaI EaeI EarI EcoNI
Hin4I MspA1I NspI PflMI PsrI SfiI SmlI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbeI BcgI BciVI BfrBI BglII
BlpI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EagI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI