Restriction map for
pF1KB5017
(Linear) (Six-base) MAPSORT of: KIBB5017.seq Check: 915 from: 1 to: 1014
KIBB5017.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 972 1014
Size: 557 415 42
Fragments arranged by size:
557 415 42
AflIII A'CryG_T
Cuts at: 0 860 1014
Size: 860 154
AleI CACnn'nnGTG
Cuts at: 0 13 1014
Size: 13 1001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 817 1014
Size: 114 703 197
Fragments arranged by size:
703 197 114
AlwNI CAG_nnn'CTG
Cuts at: 0 771 1014
Size: 771 243
ApaI G_GGCC'C
Cuts at: 0 512 564 1014
Size: 512 52 450
Fragments arranged by size:
512 450 52
AvaI C'yCGr_G
Cuts at: 0 313 563 805 1014
Size: 313 250 242 209
Fragments arranged by size:
313 250 242 209
BanI G'GyrC_C
Cuts at: 0 124 379 736 1014
Size: 124 255 357 278
Fragments arranged by size:
357 278 255 124
BanII G_rGCy'C
Cuts at: 0 241 431 512 564 718 1014
Size: 241 190 81 52 154 296
Fragments arranged by size:
296 241 190 154 81 52
BbvCI CC'TCA_GC
Cuts at: 0 202 1014
Size: 202 812
BglI GCCn_nnn'nGGC
Cuts at: 0 941 1014
Size: 941 73
Bme1580I G_kGCm'C
Cuts at: 0 127 512 552 564 1014
Size: 127 385 40 12 450
Fragments arranged by size:
450 385 127 40 12
BmgBI CAC'GTC
Cuts at: 0 671 1014
Size: 671 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 80 617 1014
Size: 80 537 397
Fragments arranged by size:
537 397 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 577 744 909 1014
Size: 318 259 167 165 105
Fragments arranged by size:
318 259 167 165 105
Bpu10I CC'TnA_GC
Cuts at: 0 202 916 1014
Size: 202 714 98
Fragments arranged by size:
714 202 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 196 954 1014
Size: 57 139 758 60
Fragments arranged by size:
758 139 60 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 254 1014
Size: 254 760
BsaHI Gr'CG_yC
Cuts at: 0 975 1014
Size: 975 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 1014
Size: 190 824
BseYI C'CCAG_C
Cuts at: 0 172 613 877 1014
Size: 172 441 264 137
Fragments arranged by size:
441 264 172 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1014
Size: 110 904
BsiHKAI G_wGCw'C
Cuts at: 0 84 241 431 1014
Size: 84 157 190 583
Fragments arranged by size:
583 190 157 84
Bsp1286I G_dGCh'C
Cuts at: 0 84 127 241 431 512 552 564 718
Size: 84 43 114 190 81 40 12 154
Cuts at: 718 1014
Size: 296
Fragments arranged by size:
296 190 154 114 84 81 43 40
12
BsrBI CCG'CTC
Cuts at: 0 232 266 288 1014
Size: 232 34 22 726
Fragments arranged by size:
726 232 34 22
BssSI C'ACGA_G
Cuts at: 0 13 424 1014
Size: 13 411 590
Fragments arranged by size:
590 411 13
Bsu36I CC'TnA_GG
Cuts at: 0 884 1014
Size: 884 130
BtsI GCAGTG_nn'
Cuts at: 0 125 1014
Size: 125 889
EaeI y'GGCC_r
Cuts at: 0 321 554 1014
Size: 321 233 460
Fragments arranged by size:
460 321 233
EarI CTCTTCn'nnn_
Cuts at: 0 533 838 1014
Size: 533 305 176
Fragments arranged by size:
533 305 176
EcoICRI GAG'CTC
Cuts at: 0 239 429 1014
Size: 239 190 585
Fragments arranged by size:
585 239 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 557 577 744 909 972 1014
Size: 318 239 20 167 165 63 42
Fragments arranged by size:
318 239 167 165 63 42 20
EcoO109I rG'GnC_Cy
Cuts at: 0 509 560 567 1014
Size: 509 51 7 447
Fragments arranged by size:
509 447 51 7
HaeII r_GCGC'y
Cuts at: 0 546 701 1014
Size: 546 155 313
Fragments arranged by size:
546 313 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 880 912 986 1014
Size: 880 32 74 28
Fragments arranged by size:
880 74 32 28
HincII GTy'rAC
Cuts at: 0 330 1014
Size: 330 684
MslI CAynn'nnrTG
Cuts at: 0 13 1014
Size: 13 1001
MspA1I CmG'CkG
Cuts at: 0 515 577 631 643 1014
Size: 515 62 54 12 371
Fragments arranged by size:
515 371 62 54 12
NruI TCG'CGA
Cuts at: 0 243 1014
Size: 243 771
NspI r_CATG'y
Cuts at: 0 864 1014
Size: 864 150
PciI A'CATG_T
Cuts at: 0 860 1014
Size: 860 154
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1014
Size: 771 243
PpuMI rG'GwC_Cy
Cuts at: 0 567 1014
Size: 567 447
PspOMI G'GGCC_C
Cuts at: 0 508 560 1014
Size: 508 52 454
Fragments arranged by size:
508 454 52
SacI G_AGCT'C
Cuts at: 0 241 431 1014
Size: 241 190 583
Fragments arranged by size:
583 241 190
SapI GCTCTTCn'nnn_
Cuts at: 0 533 1014
Size: 533 481
SfcI C'TryA_G
Cuts at: 0 75 1014
Size: 75 939
SmaI CCC'GGG
Cuts at: 0 315 565 807 1014
Size: 315 250 242 207
Fragments arranged by size:
315 250 242 207
SmlI C'TyrA_G
Cuts at: 0 36 211 969 1014
Size: 36 175 758 45
Fragments arranged by size:
758 175 45 36
StyI C'CwwG_G
Cuts at: 0 959 1014
Size: 959 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 1014
Size: 316 698
TatI w'GTAC_w
Cuts at: 0 455 1014
Size: 455 559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 404 436 1014
Size: 404 32 578
Fragments arranged by size:
578 404 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 768 1014
Size: 654 114 246
Fragments arranged by size:
654 246 114
XmaI C'CCGG_G
Cuts at: 0 313 563 805 1014
Size: 313 250 242 209
Fragments arranged by size:
313 250 242 209
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI AvaI BanI
BanII BbvCI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrBI BssSI Bsu36I BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MslI MspA1I NruI NspI
PciI PflMI PpuMI PspOMI SacI SapI SfcI SmaI
SmlI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmnI ZraI