(Linear) (Six-base) MAPSORT of: KIBB5021.seq  Check: 3747  from: 1  to: 1488



KIBB5021.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AccI GT'mk_AC

Cuts at:      0     848    1488 
   Size:        848     640
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     229     407    1010    1454    1488 
   Size:        126     103     178     603     444      34

  Fragments arranged by size: 

                 603     444     178     126     103      34
 
AflIII A'CryG_T

Cuts at:      0     894    1488 
   Size:        894     594
 
AhdI GACnn_n'nnGTC

Cuts at:      0     898    1488 
   Size:        898     590
 
ApoI r'AATT_y

Cuts at:      0     286     556    1235    1488 
   Size:        286     270     679     253

  Fragments arranged by size: 

                 679     286     270     253
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     961     994    1488 
   Size:        961      33     494

  Fragments arranged by size: 

                 961     494      33
 
BanI G'GyrC_C

Cuts at:      0    1034    1213    1488 
   Size:       1034     179     275

  Fragments arranged by size: 

                1034     275     179
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     132    1229    1481    1488 
   Size:        132    1097     252       7

  Fragments arranged by size: 

                1097     252     132       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     320    1488 
   Size:        320    1168
 
BfrBI ATG'CAT

Cuts at:      0    1441    1488 
   Size:       1441      47
 
Bpu10I CC'TnA_GC

Cuts at:      0     776    1488 
   Size:        776     712
 
BseYI C'CCAG_C

Cuts at:      0      55    1268    1488 
   Size:         55    1213     220

  Fragments arranged by size: 

                1213     220      55
 
BsiHKAI G_wGCw'C

Cuts at:      0     641     782    1488 
   Size:        641     141     706

  Fragments arranged by size: 

                 706     641     141
 
BsmI GAATG_Cn'

Cuts at:      0      50     343    1419    1441    1488 
   Size:         50     293    1076      22      47

  Fragments arranged by size: 

                1076     293      50      47      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     641     782    1488 
   Size:        641     141     706

  Fragments arranged by size: 

                 706     641     141
 
BsrGI T'GTAC_A

Cuts at:      0    1281    1488 
   Size:       1281     207
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1438    1488 
   Size:       1438      50
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1425    1488 
   Size:       1425      63
 
BstYI r'GATC_y

Cuts at:      0     378    1488 
   Size:        378    1110
 
BstZ17I GTA'TAC

Cuts at:      0     849    1488 
   Size:        849     639
 
BtgI C'CryG_G

Cuts at:      0      17    1488 
   Size:         17    1471
 
DraI TTT'AAA

Cuts at:      0     182    1488 
   Size:        182    1306
 
EarI CTCTTCn'nnn_

Cuts at:      0      79    1488 
   Size:         79    1409
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     126     229     407    1010    1454    1488 
   Size:        126     103     178     603     444      34

  Fragments arranged by size: 

                 603     444     178     126     103      34
 
EcoRI G'AATT_C

Cuts at:      0     286    1488 
   Size:        286    1202
 
EcoRV GAT'ATC

Cuts at:      0    1335    1488 
   Size:       1335     153
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1242    1246    1488 
   Size:       1242       4     242

  Fragments arranged by size: 

                1242     242       4
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     884     916    1488 
   Size:        884      32     572

  Fragments arranged by size: 

                 884     572      32
 
HindIII A'AGCT_T

Cuts at:      0     703    1488 
   Size:        703     785
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     651    1488 
   Size:        651     837
 
MslI CAynn'nnrTG

Cuts at:      0     332    1488 
   Size:        332    1156
 
MspA1I CmG'CkG

Cuts at:      0    1268    1488 
   Size:       1268     220
 
NcoI C'CATG_G

Cuts at:      0      17    1488 
   Size:         17    1471
 
NsiI A_TGCA'T

Cuts at:      0    1443    1488 
   Size:       1443      45
 
NspI r_CATG'y

Cuts at:      0     898    1488 
   Size:        898     590
 
PciI A'CATG_T

Cuts at:      0     894    1488 
   Size:        894     594
 
PsiI TTA'TAA

Cuts at:      0     101     173     323    1488 
   Size:        101      72     150    1165

  Fragments arranged by size: 

                1165     150     101      72
 
PvuII CAG'CTG

Cuts at:      0    1268    1488 
   Size:       1268     220
 
SfcI C'TryA_G

Cuts at:      0    1022    1406    1488 
   Size:       1022     384      82

  Fragments arranged by size: 

                1022     384      82
 
SspI AAT'ATT

Cuts at:      0     230     873    1488 
   Size:        230     643     615

  Fragments arranged by size: 

                 643     615     230
 
StyI C'CwwG_G

Cuts at:      0      17    1038    1217    1488 
   Size:         17    1021     179     271

  Fragments arranged by size: 

                1021     271     179      17
 
TatI w'GTAC_w

Cuts at:      0    1281    1488 
   Size:       1281     207
 
XmnI GAAnn'nnTTC

Cuts at:      0     290     872    1226    1370    1488 
   Size:        290     582     354     144     118

  Fragments arranged by size: 

                 582     354     290     144     118
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     ApoI     BaeI     BanI     BbsI
    BciVI    BfrBI   Bpu10I    BseYI  BsiHKAI     BsmI Bsp1286I    BsrGI
   BstAPI    BstXI    BstYI  BstZ17I     BtgI     DraI     EarI  Eco57MI
    EcoRI    EcoRV     FalI    Hin4I  HindIII     MmeI     MslI   MspA1I
     NcoI     NsiI     NspI     PciI     PsiI    PvuII     SfcI     SspI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI