Restriction map for
pF1KB5023
(Linear) (Six-base) MAPSORT of: KIBB5023.seq Check: 1769 from: 1 to: 987
KIBB5023.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 58 987
Size: 58 929
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 987
Size: 437 32 518
Fragments arranged by size:
518 437 32
AlwNI CAG_nnn'CTG
Cuts at: 0 431 987
Size: 431 556
ApoI r'AATT_y
Cuts at: 0 43 832 987
Size: 43 789 155
Fragments arranged by size:
789 155 43
BanII G_rGCy'C
Cuts at: 0 417 582 987
Size: 417 165 405
Fragments arranged by size:
417 405 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 697 987
Size: 697 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 770 804 987
Size: 770 34 183
Fragments arranged by size:
770 183 34
BfrBI ATG'CAT
Cuts at: 0 130 250 987
Size: 130 120 737
Fragments arranged by size:
737 130 120
Bme1580I G_kGCm'C
Cuts at: 0 535 987
Size: 535 452
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 826 987
Size: 826 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 987
Size: 464 523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 987
Size: 720 267
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 710 740 987
Size: 437 30 243 30 247
Fragments arranged by size:
437 247 243 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 491 987
Size: 491 496
BseYI C'CCAG_C
Cuts at: 0 220 987
Size: 220 767
BsiHKAI G_wGCw'C
Cuts at: 0 417 987
Size: 417 570
BsmI GAATG_Cn'
Cuts at: 0 389 987
Size: 389 598
Bsp1286I G_dGCh'C
Cuts at: 0 417 535 582 987
Size: 417 118 47 405
Fragments arranged by size:
417 405 118 47
BspHI T'CATG_A
Cuts at: 0 933 987
Size: 933 54
BssSI C'ACGA_G
Cuts at: 0 767 987
Size: 767 220
BstAPI GCAn_nnn'nTGC
Cuts at: 0 316 987
Size: 316 671
BstXI CCAn_nnnn'nTGG
Cuts at: 0 133 987
Size: 133 854
BtgI C'CryG_G
Cuts at: 0 146 776 987
Size: 146 630 211
Fragments arranged by size:
630 211 146
BtsI GCAGTG_nn'
Cuts at: 0 533 987
Size: 533 454
DraI TTT'AAA
Cuts at: 0 369 987
Size: 369 618
EaeI y'GGCC_r
Cuts at: 0 923 987
Size: 923 64
EcoICRI GAG'CTC
Cuts at: 0 415 987
Size: 415 572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 987
Size: 464 523
EcoNI CCTnn'n_nnAGG
Cuts at: 0 893 987
Size: 893 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 235 267 368 400 404 436
Size: 44 32 159 32 101 32 4 32
Cuts at: 436 885 917 987
Size: 449 32 70
Fragments arranged by size:
449 159 101 70 44 32 32 32
32 32 4
HincII GTy'rAC
Cuts at: 0 141 987
Size: 141 846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 462 987
Size: 219 243 525
Fragments arranged by size:
525 243 219
MslI CAynn'nnrTG
Cuts at: 0 131 953 987
Size: 131 822 34
Fragments arranged by size:
822 131 34
NcoI C'CATG_G
Cuts at: 0 776 987
Size: 776 211
NsiI A_TGCA'T
Cuts at: 0 132 252 987
Size: 132 120 735
Fragments arranged by size:
735 132 120
NspI r_CATG'y
Cuts at: 0 134 497 987
Size: 134 363 490
Fragments arranged by size:
490 363 134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 437 469 987
Size: 437 32 518
Fragments arranged by size:
518 437 32
PshAI GACnn'nnGTC
Cuts at: 0 701 987
Size: 701 286
SacI G_AGCT'C
Cuts at: 0 417 987
Size: 417 570
SmlI C'TyrA_G
Cuts at: 0 699 987
Size: 699 288
SphI G_CATG'C
Cuts at: 0 497 987
Size: 497 490
StyI C'CwwG_G
Cuts at: 0 776 824 987
Size: 776 48 163
Fragments arranged by size:
776 163 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 710 742 987
Size: 710 32 245
Fragments arranged by size:
710 245 32
Enzymes that do cut:
AhdI AloI AlwNI ApoI BanII BbsI BcgI BfrBI
Bme1580I BplI BpmI BpuEI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BssSI BstAPI BstXI BtgI BtsI
DraI EaeI EcoICRI Eco57MI EcoNI Hin4I HincII MmeI
MslI NcoI NsiI NspI PpiI PshAI SacI SmlI
SphI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EarI EciI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI