Restriction map for
pF1KB5024
(Linear) (Six-base) MAPSORT of: KIBB5024.seq Check: 9084 from: 1 to: 1284
KIBB5024.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1269 1284
Size: 1269 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1284
Size: 660 624
AflIII A'CryG_T
Cuts at: 0 80 467 1284
Size: 80 387 817
Fragments arranged by size:
817 387 80
AleI CACnn'nnGTG
Cuts at: 0 1231 1273 1284
Size: 1231 42 11
Fragments arranged by size:
1231 42 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 548 1284
Size: 548 736
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 598 630 1284
Size: 598 32 654
Fragments arranged by size:
654 598 32
AlwNI CAG_nnn'CTG
Cuts at: 0 273 318 418 946 1012 1284
Size: 273 45 100 528 66 272
Fragments arranged by size:
528 273 272 100 66 45
ApaI G_GGCC'C
Cuts at: 0 26 738 1284
Size: 26 712 546
Fragments arranged by size:
712 546 26
AvaI C'yCGr_G
Cuts at: 0 343 1284
Size: 343 941
BanII G_rGCy'C
Cuts at: 0 26 496 738 845 1284
Size: 26 470 242 107 439
Fragments arranged by size:
470 439 242 107 26
BbvCI CC'TCA_GC
Cuts at: 0 448 704 1284
Size: 448 256 580
Fragments arranged by size:
580 448 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1189 1223 1284
Size: 1189 34 61
Fragments arranged by size:
1189 61 34
BclI T'GATC_A
Cuts at: 0 1256 1284
Size: 1256 28
BglI GCCn_nnn'nGGC
Cuts at: 0 434 715 811 1069 1284
Size: 434 281 96 258 215
Fragments arranged by size:
434 281 258 215 96
BlpI GC'TnA_GC
Cuts at: 0 1013 1284
Size: 1013 271
Bme1580I G_kGCm'C
Cuts at: 0 26 738 1284
Size: 26 712 546
Fragments arranged by size:
712 546 26
BmgBI CAC'GTC
Cuts at: 0 468 1284
Size: 468 816
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 1284
Size: 705 579
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1284
Size: 340 944
Bpu10I CC'TnA_GC
Cuts at: 0 448 704 1284
Size: 448 256 580
Fragments arranged by size:
580 448 256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1284
Size: 1012 272
BsaAI yAC'GTr
Cuts at: 0 81 1284
Size: 81 1203
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 1284
Size: 697 587
BseYI C'CCAG_C
Cuts at: 0 92 1284
Size: 92 1192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 544 805 1284
Size: 307 237 261 479
Fragments arranged by size:
479 307 261 237
BsiEI CG_ry'CG
Cuts at: 0 185 1284
Size: 185 1099
BsiHKAI G_wGCw'C
Cuts at: 0 496 845 1284
Size: 496 349 439
Fragments arranged by size:
496 439 349
BsiWI C'GTAC_G
Cuts at: 0 829 1284
Size: 829 455
BsmBI CGTCTCn'nnnn_
Cuts at: 0 172 1284
Size: 172 1112
Bsp1286I G_dGCh'C
Cuts at: 0 26 496 738 845 1284
Size: 26 470 242 107 439
Fragments arranged by size:
470 439 242 107 26
BspHI T'CATG_A
Cuts at: 0 230 1284
Size: 230 1054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 912 1269 1284
Size: 912 357 15
Fragments arranged by size:
912 357 15
BsrBI CCG'CTC
Cuts at: 0 1017 1284
Size: 1017 267
BsrFI r'CCGG_y
Cuts at: 0 860 1284
Size: 860 424
BstYI r'GATC_y
Cuts at: 0 443 752 821 953 1284
Size: 443 309 69 132 331
Fragments arranged by size:
443 331 309 132 69
BtgI C'CryG_G
Cuts at: 0 254 518 806 1284
Size: 254 264 288 478
Fragments arranged by size:
478 288 264 254
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 831 1009 1284
Size: 831 178 275
Fragments arranged by size:
831 275 178
DraIII CAC_nnn'GTG
Cuts at: 0 18 1284
Size: 18 1266
EaeI y'GGCC_r
Cuts at: 0 182 251 426 548 1196 1284
Size: 182 69 175 122 648 88
Fragments arranged by size:
648 182 175 122 88 69
EagI C'GGCC_G
Cuts at: 0 182 1284
Size: 182 1102
EarI CTCTTCn'nnn_
Cuts at: 0 758 1284
Size: 758 526
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 988 1284
Size: 988 296
EcoICRI GAG'CTC
Cuts at: 0 494 843 1284
Size: 494 349 441
Fragments arranged by size:
494 441 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 660 1284
Size: 340 320 624
Fragments arranged by size:
624 340 320
EcoO109I rG'GnC_Cy
Cuts at: 0 22 347 734 924 1086 1284
Size: 22 325 387 190 162 198
Fragments arranged by size:
387 325 198 190 162 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 669 690 1089 1284
Size: 669 21 399 195
Fragments arranged by size:
669 399 195 21
HaeII r_GCGC'y
Cuts at: 0 37 1284
Size: 37 1247
HincII GTy'rAC
Cuts at: 0 690 1284
Size: 690 594
HpaI GTT'AAC
Cuts at: 0 690 1284
Size: 690 594
MfeI C'AATT_G
Cuts at: 0 6 1284
Size: 6 1278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 143 1284
Size: 89 54 1141
Fragments arranged by size:
1141 89 54
MslI CAynn'nnrTG
Cuts at: 0 544 1231 1273 1284
Size: 544 687 42 11
Fragments arranged by size:
687 544 42 11
MspA1I CmG'CkG
Cuts at: 0 164 703 1284
Size: 164 539 581
Fragments arranged by size:
581 539 164
NaeI GCC'GGC
Cuts at: 0 862 1284
Size: 862 422
NgoMIV G'CCGG_C
Cuts at: 0 860 1284
Size: 860 424
NspI r_CATG'y
Cuts at: 0 1222 1284
Size: 1222 62
PasI CC'CwG_GG
Cuts at: 0 498 1284
Size: 498 786
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 1284
Size: 18 1266
PfoI T'CCnGG_A
Cuts at: 0 575 1284
Size: 575 709
PpuMI rG'GwC_Cy
Cuts at: 0 347 924 1284
Size: 347 577 360
Fragments arranged by size:
577 360 347
PshAI GACnn'nnGTC
Cuts at: 0 211 1284
Size: 211 1073
PspOMI G'GGCC_C
Cuts at: 0 22 734 1284
Size: 22 712 550
Fragments arranged by size:
712 550 22
PstI C_TGCA'G
Cuts at: 0 314 731 1284
Size: 314 417 553
Fragments arranged by size:
553 417 314
PvuII CAG'CTG
Cuts at: 0 703 1284
Size: 703 581
SacI G_AGCT'C
Cuts at: 0 496 845 1284
Size: 496 349 439
Fragments arranged by size:
496 439 349
SanDI GG'GwC_CC
Cuts at: 0 347 1284
Size: 347 937
SfcI C'TryA_G
Cuts at: 0 310 727 851 1284
Size: 310 417 124 433
Fragments arranged by size:
433 417 310 124
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 715 1284
Size: 715 569
SmlI C'TyrA_G
Cuts at: 0 1027 1284
Size: 1027 257
SphI G_CATG'C
Cuts at: 0 1222 1284
Size: 1222 62
StyI C'CwwG_G
Cuts at: 0 1073 1127 1284
Size: 1073 54 157
Fragments arranged by size:
1073 157 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 571 603 806 838 1284
Size: 571 32 203 32 446
Fragments arranged by size:
571 446 203 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 1071 1284
Size: 33 1038 213
Fragments arranged by size:
1038 213 33
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI
AvaI BanII BbvCI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BstYI BtgI BtgZI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII HincII
HpaI MfeI MmeI MslI MspA1I NaeI NgoMIV NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SfcI SfiI SmlI SphI StyI TstI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BciVI BfrBI BglII BmtI BplI
BsaI BsaBI BsaHI BsaWI BsaXI BsmI BspEI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DrdI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HindIII KasI KpnI
MluI MscI NarI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI