Restriction map for
pF1KB5026
(Linear) (Six-base) MAPSORT of: KIBB5026.seq Check: 2987 from: 1 to: 1344
KIBB5026.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 539 1344
Size: 539 805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 179 379 598 1250 1344
Size: 105 74 200 219 652 94
Fragments arranged by size:
652 219 200 105 94 74
AflIII A'CryG_T
Cuts at: 0 771 1344
Size: 771 573
AlwNI CAG_nnn'CTG
Cuts at: 0 48 153 1113 1228 1344
Size: 48 105 960 115 116
Fragments arranged by size:
960 116 115 105 48
ApaI G_GGCC'C
Cuts at: 0 1340 1344
Size: 1340 4
ApaLI G'TGCA_C
Cuts at: 0 97 1344
Size: 97 1247
ApoI r'AATT_y
Cuts at: 0 641 724 927 1001 1344
Size: 641 83 203 74 343
Fragments arranged by size:
641 343 203 83 74
AvaI C'yCGr_G
Cuts at: 0 371 558 1344
Size: 371 187 786
Fragments arranged by size:
786 371 187
BanI G'GyrC_C
Cuts at: 0 969 1344
Size: 969 375
BanII G_rGCy'C
Cuts at: 0 1340 1344
Size: 1340 4
BbeI G_GCGC'C
Cuts at: 0 973 1344
Size: 973 371
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 1344
Size: 434 910
Bme1580I G_kGCm'C
Cuts at: 0 101 1340 1344
Size: 101 1239 4
Fragments arranged by size:
1239 101 4
BmgBI CAC'GTC
Cuts at: 0 849 1344
Size: 849 495
BmtI G_CTAG'C
Cuts at: 0 13 1344
Size: 13 1331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1090 1333 1344
Size: 1038 52 243 11
Fragments arranged by size:
1038 243 52 11
Bpu10I CC'TnA_GC
Cuts at: 0 1023 1344
Size: 1023 321
BsaBI GATnn'nnATC
Cuts at: 0 200 1344
Size: 200 1144
BsaHI Gr'CG_yC
Cuts at: 0 970 1344
Size: 970 374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 521 551 1344
Size: 521 30 793
Fragments arranged by size:
793 521 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1330 1344
Size: 1330 14
BseYI C'CCAG_C
Cuts at: 0 399 1344
Size: 399 945
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 1344
Size: 1279 65
BsiHKAI G_wGCw'C
Cuts at: 0 101 1344
Size: 101 1243
BsmBI CGTCTCn'nnnn_
Cuts at: 0 839 1344
Size: 839 505
Bsp1286I G_dGCh'C
Cuts at: 0 101 1340 1344
Size: 101 1239 4
Fragments arranged by size:
1239 101 4
BsrDI GCAATG_nn'
Cuts at: 0 245 1344
Size: 245 1099
BsrGI T'GTAC_A
Cuts at: 0 718 1176 1344
Size: 718 458 168
Fragments arranged by size:
718 458 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 1344
Size: 267 1077
BstYI r'GATC_y
Cuts at: 0 65 739 1090 1344
Size: 65 674 351 254
Fragments arranged by size:
674 351 254 65
BtgI C'CryG_G
Cuts at: 0 32 1283 1344
Size: 32 1251 61
Fragments arranged by size:
1251 61 32
BtsI GCAGTG_nn'
Cuts at: 0 1123 1344
Size: 1123 221
DraI TTT'AAA
Cuts at: 0 830 1344
Size: 830 514
DraIII CAC_nnn'GTG
Cuts at: 0 1193 1344
Size: 1193 151
EaeI y'GGCC_r
Cuts at: 0 474 1344
Size: 474 870
EarI CTCTTCn'nnn_
Cuts at: 0 124 1344
Size: 124 1220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1338 1344
Size: 1338 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 179 379 598 1038 1090 1250 1333
Size: 105 74 200 219 440 52 160 83
Cuts at: 1333 1344
Size: 11
Fragments arranged by size:
440 219 200 160 105 83 74 52
11
EcoO109I rG'GnC_Cy
Cuts at: 0 1336 1337 1344
Size: 1336 1 7
Fragments arranged by size:
1336 7 1
EcoRI G'AATT_C
Cuts at: 0 724 927 1001 1344
Size: 724 203 74 343
Fragments arranged by size:
724 343 203 74
EcoRV GAT'ATC
Cuts at: 0 502 1344
Size: 502 842
HaeII r_GCGC'y
Cuts at: 0 973 1312 1344
Size: 973 339 32
Fragments arranged by size:
973 339 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 724 756 1344
Size: 521 32 171 32 588
Fragments arranged by size:
588 521 171 32 32
KasI G'GCGC_C
Cuts at: 0 969 1344
Size: 969 375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 930 1134 1344
Size: 930 204 210
Fragments arranged by size:
930 210 204
MscI TGG'CCA
Cuts at: 0 476 1344
Size: 476 868
MslI CAynn'nnrTG
Cuts at: 0 265 1010 1297 1344
Size: 265 745 287 47
Fragments arranged by size:
745 287 265 47
MspA1I CmG'CkG
Cuts at: 0 921 1344
Size: 921 423
NarI GG'CG_CC
Cuts at: 0 970 1344
Size: 970 374
NcoI C'CATG_G
Cuts at: 0 32 1344
Size: 32 1312
NheI G'CTAG_C
Cuts at: 0 9 1344
Size: 9 1335
NspI r_CATG'y
Cuts at: 0 775 1344
Size: 775 569
PciI A'CATG_T
Cuts at: 0 771 1344
Size: 771 573
PflMI CCAn_nnn'nTGG
Cuts at: 0 48 76 1344
Size: 48 28 1268
Fragments arranged by size:
1268 48 28
PsiI TTA'TAA
Cuts at: 0 650 1344
Size: 650 694
PspOMI G'GGCC_C
Cuts at: 0 1336 1344
Size: 1336 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 332 364 479 511 1344
Size: 332 32 115 32 833
Fragments arranged by size:
833 332 115 32 32
PstI C_TGCA'G
Cuts at: 0 308 1118 1344
Size: 308 810 226
Fragments arranged by size:
810 308 226
PvuII CAG'CTG
Cuts at: 0 921 1344
Size: 921 423
SexAI A'CCwGG_T
Cuts at: 0 41 1344
Size: 41 1303
SfcI C'TryA_G
Cuts at: 0 170 304 932 1114 1344
Size: 170 134 628 182 230
Fragments arranged by size:
628 230 182 170 134
SfoI GGC'GCC
Cuts at: 0 971 1344
Size: 971 373
SspI AAT'ATT
Cuts at: 0 572 1344
Size: 572 772
StuI AGG'CCT
Cuts at: 0 809 1344
Size: 809 535
StyI C'CwwG_G
Cuts at: 0 15 32 777 1344
Size: 15 17 745 567
Fragments arranged by size:
745 567 17 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1287 1344
Size: 1287 57
TatI w'GTAC_w
Cuts at: 0 718 1176 1344
Size: 718 458 168
Fragments arranged by size:
718 458 168
XmnI GAAnn'nnTTC
Cuts at: 0 1005 1344
Size: 1005 339
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI
BanI BanII BbeI BbsI Bme1580I BmgBI BmtI BpmI
Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BsrDI BsrGI BstXI BstYI BtgI BtsI
DraI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NcoI NheI NspI PciI PflMI PsiI PspOMI
PsrI PstI PvuII SexAI SfcI SfoI SspI StuI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmrI BplI BpuEI BsaI BsaAI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DrdI EagI EcoICRI EcoNI FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI