Restriction map for
pF1KB5029
(Linear) (Six-base) MAPSORT of: KIBB5029.seq Check: 5276 from: 1 to: 1644
KIBB5029.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1073 1644
Size: 1073 571
AccI GT'mk_AC
Cuts at: 0 1556 1644
Size: 1556 88
Acc65I G'GTAC_C
Cuts at: 0 1361 1644
Size: 1361 283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1294 1563 1644
Size: 464 830 269 81
Fragments arranged by size:
830 464 269 81
AflIII A'CryG_T
Cuts at: 0 338 1644
Size: 338 1306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 421 1644
Size: 421 1223
AlwNI CAG_nnn'CTG
Cuts at: 0 363 1208 1236 1644
Size: 363 845 28 408
Fragments arranged by size:
845 408 363 28
ApaI G_GGCC'C
Cuts at: 0 594 1644
Size: 594 1050
AvaI C'yCGr_G
Cuts at: 0 64 470 1644
Size: 64 406 1174
Fragments arranged by size:
1174 406 64
AvrII C'CTAG_G
Cuts at: 0 1395 1644
Size: 1395 249
BanI G'GyrC_C
Cuts at: 0 1361 1399 1573 1644
Size: 1361 38 174 71
Fragments arranged by size:
1361 174 71 38
BanII G_rGCy'C
Cuts at: 0 594 893 1644
Size: 594 299 751
Fragments arranged by size:
751 594 299
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 1644
Size: 274 1370
BciVI GTATCCnnnnn_n'
Cuts at: 0 243 1644
Size: 243 1401
BglI GCCn_nnn'nGGC
Cuts at: 0 754 943 1644
Size: 754 189 701
Fragments arranged by size:
754 701 189
Bme1580I G_kGCm'C
Cuts at: 0 594 1644
Size: 594 1050
BmgBI CAC'GTC
Cuts at: 0 1389 1644
Size: 1389 255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1522 1644
Size: 1166 356 122
Fragments arranged by size:
1166 356 122
Bpu10I CC'TnA_GC
Cuts at: 0 1444 1644
Size: 1444 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1002 1324 1510 1644
Size: 881 121 322 186 134
Fragments arranged by size:
881 322 186 134 121
BsaAI yAC'GTr
Cuts at: 0 1368 1644
Size: 1368 276
BsaHI Gr'CG_yC
Cuts at: 0 1136 1227 1644
Size: 1136 91 417
Fragments arranged by size:
1136 417 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 952 982 1644
Size: 952 30 662
Fragments arranged by size:
952 662 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 168 235 648 1368 1519 1612 1621 1624
Size: 168 67 413 720 151 93 9 3
Cuts at: 1624 1644
Size: 20
Fragments arranged by size:
720 413 168 151 93 67 20 9
3
BseYI C'CCAG_C
Cuts at: 0 44 971 1403 1644
Size: 44 927 432 241
Fragments arranged by size:
927 432 241 44
BsiHKAI G_wGCw'C
Cuts at: 0 1130 1644
Size: 1130 514
BsmI GAATG_Cn'
Cuts at: 0 398 1461 1644
Size: 398 1063 183
Fragments arranged by size:
1063 398 183
Bsp1286I G_dGCh'C
Cuts at: 0 594 893 1130 1644
Size: 594 299 237 514
Fragments arranged by size:
594 514 299 237
BspHI T'CATG_A
Cuts at: 0 11 1139 1644
Size: 11 1128 505
Fragments arranged by size:
1128 505 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 1073 1315 1533 1644
Size: 362 711 242 218 111
Fragments arranged by size:
711 362 242 218 111
BsrBI CCG'CTC
Cuts at: 0 779 1644
Size: 779 865
BsrDI GCAATG_nn'
Cuts at: 0 1259 1644
Size: 1259 385
BssHII G'CGCG_C
Cuts at: 0 256 1644
Size: 256 1388
BssSI C'ACGA_G
Cuts at: 0 630 1644
Size: 630 1014
BstAPI GCAn_nnn'nTGC
Cuts at: 0 363 1644
Size: 363 1281
BstXI CCAn_nnnn'nTGG
Cuts at: 0 814 1644
Size: 814 830
BstYI r'GATC_y
Cuts at: 0 359 1327 1644
Size: 359 968 317
Fragments arranged by size:
968 359 317
Bsu36I CC'TnA_GG
Cuts at: 0 39 1644
Size: 39 1605
BtgI C'CryG_G
Cuts at: 0 938 1265 1644
Size: 938 327 379
Fragments arranged by size:
938 379 327
BtsI GCAGTG_nn'
Cuts at: 0 646 982 1644
Size: 646 336 662
Fragments arranged by size:
662 646 336
DrdI GACnn_nn'nnGTC
Cuts at: 0 697 1114 1644
Size: 697 417 530
Fragments arranged by size:
697 530 417
EaeI y'GGCC_r
Cuts at: 0 755 935 1644
Size: 755 180 709
Fragments arranged by size:
755 709 180
EarI CTCTTCn'nnn_
Cuts at: 0 483 832 1598 1613 1625 1644
Size: 483 349 766 15 12 19
Fragments arranged by size:
766 483 349 19 15 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1166 1294 1522 1563 1644
Size: 464 702 128 228 41 81
Fragments arranged by size:
702 464 228 128 81 41
EcoO109I rG'GnC_Cy
Cuts at: 0 49 590 591 1530 1644
Size: 49 541 1 939 114
Fragments arranged by size:
939 541 114 49 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 684 1644
Size: 684 960
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 442 474 1241 1273 1644
Size: 22 32 388 32 767 32 371
Fragments arranged by size:
767 388 371 32 32 32 22
HincII GTy'rAC
Cuts at: 0 1221 1644
Size: 1221 423
HindIII A'AGCT_T
Cuts at: 0 1090 1342 1644
Size: 1090 252 302
Fragments arranged by size:
1090 302 252
KpnI G_GTAC'C
Cuts at: 0 1365 1644
Size: 1365 279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1644
Size: 313 1331
MscI TGG'CCA
Cuts at: 0 757 937 1644
Size: 757 180 707
Fragments arranged by size:
757 707 180
MslI CAynn'nnrTG
Cuts at: 0 763 1211 1492 1644
Size: 763 448 281 152
Fragments arranged by size:
763 448 281 152
MspA1I CmG'CkG
Cuts at: 0 243 282 1644
Size: 243 39 1362
Fragments arranged by size:
1362 243 39
NcoI C'CATG_G
Cuts at: 0 938 1644
Size: 938 706
NspI r_CATG'y
Cuts at: 0 342 1644
Size: 342 1302
PciI A'CATG_T
Cuts at: 0 338 1644
Size: 338 1306
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 1644
Size: 246 1398
PfoI T'CCnGG_A
Cuts at: 0 26 1644
Size: 26 1618
PmlI CAC'GTG
Cuts at: 0 1368 1644
Size: 1368 276
PpuMI rG'GwC_Cy
Cuts at: 0 49 1530 1644
Size: 49 1481 114
Fragments arranged by size:
1481 114 49
PshAI GACnn'nnGTC
Cuts at: 0 1226 1644
Size: 1226 418
PspOMI G'GGCC_C
Cuts at: 0 590 1644
Size: 590 1054
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1644
Size: 1270 32 342
Fragments arranged by size:
1270 342 32
PstI C_TGCA'G
Cuts at: 0 359 410 1644
Size: 359 51 1234
Fragments arranged by size:
1234 359 51
PvuII CAG'CTG
Cuts at: 0 243 1644
Size: 243 1401
SanDI GG'GwC_CC
Cuts at: 0 49 1644
Size: 49 1595
ScaI AGT'ACT
Cuts at: 0 175 1644
Size: 175 1469
SfcI C'TryA_G
Cuts at: 0 355 406 1557 1644
Size: 355 51 1151 87
Fragments arranged by size:
1151 355 87 51
SmlI C'TyrA_G
Cuts at: 0 896 981 1339 1525 1644
Size: 896 85 358 186 119
Fragments arranged by size:
896 358 186 119 85
StyI C'CwwG_G
Cuts at: 0 938 1395 1644
Size: 938 457 249
Fragments arranged by size:
938 457 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1417 1644
Size: 1417 227
TatI w'GTAC_w
Cuts at: 0 173 1644
Size: 173 1471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 565 597 952 984 1296 1328
Size: 8 32 525 32 355 32 312 32
Cuts at: 1328 1644
Size: 316
Fragments arranged by size:
525 355 316 312 32 32 32 32
8
Tth111I GACn'n_nGTC
Cuts at: 0 697 1644
Size: 697 947
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 427 1644
Size: 427 1217
XmnI GAAnn'nnTTC
Cuts at: 0 398 415 1644
Size: 398 17 1229
Fragments arranged by size:
1229 398 17
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
AvaI AvrII BanI BanII BbsI BciVI BglI Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DrdI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NcoI NspI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI
PstI PvuII SanDI ScaI SfcI SmlI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbeI
BbvCI BcgI BclI BfrBI BglII BlpI BmrI BmtI
BplI BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmBI
BspEI BsrFI BsrGI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PpiI PsiI PspXI PvuI
RsrII SacI SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XhoI XmaI ZraI