(Linear) (Six-base) MAPSORT of: KIBB5035.seq  Check: 2187  from: 1  to: 576



KIBB5035.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     576 
   Size:        374     202
 
ApoI r'AATT_y

Cuts at:      0     285     576 
   Size:        285     291
 
AvaI C'yCGr_G

Cuts at:      0     280     576 
   Size:        280     296
 
BanII G_rGCy'C

Cuts at:      0      10     135     576 
   Size:         10     125     441

  Fragments arranged by size: 

                 441     125      10
 
BglII A'GATC_T

Cuts at:      0      97     576 
   Size:         97     479
 
Bme1580I G_kGCm'C

Cuts at:      0      54     576 
   Size:         54     522
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     558     576 
   Size:        558      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     528     576 
   Size:        528      48
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430     576 
   Size:        430     146
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     174     576 
   Size:        174     402
 
BsiHKAI G_wGCw'C

Cuts at:      0     135     576 
   Size:        135     441
 
BsmI GAATG_Cn'

Cuts at:      0      21     576 
   Size:         21     555
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      54     135     576 
   Size:         10      44      81     441

  Fragments arranged by size: 

                 441      81      44      10
 
BstYI r'GATC_y

Cuts at:      0      97     576 
   Size:         97     479
 
Bsu36I CC'TnA_GG

Cuts at:      0     341     576 
   Size:        341     235
 
BtgI C'CryG_G

Cuts at:      0      34     453     576 
   Size:         34     419     123

  Fragments arranged by size: 

                 419     123      34
 
BtsI GCAGTG_nn'

Cuts at:      0      86     576 
   Size:         86     490
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      23      58     576 
   Size:         23      35     518

  Fragments arranged by size: 

                 518      35      23
 
DraI TTT'AAA

Cuts at:      0     311     576 
   Size:        311     265
 
EcoICRI GAG'CTC

Cuts at:      0     133     576 
   Size:        133     443
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     528     576 
   Size:        374     154      48

  Fragments arranged by size: 

                 374     154      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     337     576 
   Size:        337     239
 
MspA1I CmG'CkG

Cuts at:      0      68     330     576 
   Size:         68     262     246

  Fragments arranged by size: 

                 262     246      68
 
NcoI C'CATG_G

Cuts at:      0     453     576 
   Size:        453     123
 
PpuMI rG'GwC_Cy

Cuts at:      0     337     576 
   Size:        337     239
 
PstI C_TGCA'G

Cuts at:      0      20     335     576 
   Size:         20     315     241

  Fragments arranged by size: 

                 315     241      20
 
PvuII CAG'CTG

Cuts at:      0     330     576 
   Size:        330     246
 
SacI G_AGCT'C

Cuts at:      0     135     576 
   Size:        135     441
 
SfcI C'TryA_G

Cuts at:      0      16     331     576 
   Size:         16     315     245

  Fragments arranged by size: 

                 315     245      16
 
SmlI C'TyrA_G

Cuts at:      0     280     576 
   Size:        280     296
 
StyI C'CwwG_G

Cuts at:      0     453     576 
   Size:        453     123
 
XhoI C'TCGA_G

Cuts at:      0     280     576 
   Size:        280     296
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BanII    BglII Bme1580I     BplI     BpmI
    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BstYI   Bsu36I     BtgI
     BtsI    CspCI     DraI  EcoICRI  Eco57MI EcoO109I   MspA1I     NcoI
    PpuMI     PstI    PvuII     SacI     SfcI     SmlI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI   DraIII
     DrdI     EaeI     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI