Restriction map for
pF1KB5063
(Linear) (Six-base) MAPSORT of: KIBB5063.seq Check: 3203 from: 1 to: 2325
KIBB5063.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 1427 2325
Size: 1427 898
AccI GT'mk_AC
Cuts at: 0 1264 2325
Size: 1264 1061
AclI AA'CG_TT
Cuts at: 0 420 2325
Size: 420 1905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 2123 2323 2325
Size: 490 1633 200 2
Fragments arranged by size:
1633 490 200 2
AfeI AGC'GCT
Cuts at: 0 264 2325
Size: 264 2061
AflII C'TTAA_G
Cuts at: 0 685 2325
Size: 685 1640
AflIII A'CryG_T
Cuts at: 0 2160 2325
Size: 2160 165
AhdI GACnn_n'nnGTC
Cuts at: 0 18 2325
Size: 18 2307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 922 954 2325
Size: 547 32 343 32 1371
Fragments arranged by size:
1371 547 343 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 129 411 651 768 2008 2325
Size: 129 282 240 117 1240 317
Fragments arranged by size:
1240 317 282 240 129 117
ApaI G_GGCC'C
Cuts at: 0 535 1960 2325
Size: 535 1425 365
Fragments arranged by size:
1425 535 365
ApoI r'AATT_y
Cuts at: 0 1375 2325
Size: 1375 950
AvaI C'yCGr_G
Cuts at: 0 458 1610 2325
Size: 458 1152 715
Fragments arranged by size:
1152 715 458
BamHI G'GATC_C
Cuts at: 0 158 2253 2325
Size: 158 2095 72
Fragments arranged by size:
2095 158 72
BanI G'GyrC_C
Cuts at: 0 395 1773 2118 2325
Size: 395 1378 345 207
Fragments arranged by size:
1378 395 345 207
BanII G_rGCy'C
Cuts at: 0 254 535 1046 1094 1960 2325
Size: 254 281 511 48 866 365
Fragments arranged by size:
866 511 365 281 254 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 1827 2315 2325
Size: 1827 488 10
Fragments arranged by size:
1827 488 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 186 2325
Size: 186 2139
BclI T'GATC_A
Cuts at: 0 491 2325
Size: 491 1834
BlpI GC'TnA_GC
Cuts at: 0 119 259 2325
Size: 119 140 2066
Fragments arranged by size:
2066 140 119
Bme1580I G_kGCm'C
Cuts at: 0 535 1611 1778 1960 2325
Size: 535 1076 167 182 365
Fragments arranged by size:
1076 535 365 182 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 1539 1955 2046 2325
Size: 1539 416 91 279
Fragments arranged by size:
1539 416 279 91
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1108 2325
Size: 1108 1217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 790 1143 1483 1735 2002 2030 2325
Size: 574 216 353 340 252 267 28 295
Fragments arranged by size:
574 353 340 295 267 252 216 28
Bpu10I CC'TnA_GC
Cuts at: 0 43 2325
Size: 43 2282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 292 2325
Size: 55 237 2033
Fragments arranged by size:
2033 237 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 1121 1789 2325
Size: 1121 668 536
Fragments arranged by size:
1121 668 536
BsaAI yAC'GTr
Cuts at: 0 793 2163 2325
Size: 793 1370 162
Fragments arranged by size:
1370 793 162
BsaHI Gr'CG_yC
Cuts at: 0 201 1424 2325
Size: 201 1223 901
Fragments arranged by size:
1223 901 201
BsaWI w'CCGG_w
Cuts at: 0 599 2325
Size: 599 1726
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1116 1146 1610 1640 2325
Size: 547 30 539 30 464 30 685
Fragments arranged by size:
685 547 539 464 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 1097 1102 1156 1876 2325
Size: 382 715 5 54 720 449
Fragments arranged by size:
720 715 449 382 54 5
BseYI C'CCAG_C
Cuts at: 0 254 404 1785 2325
Size: 254 150 1381 540
Fragments arranged by size:
1381 540 254 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1350 1450 1459 2325
Size: 1350 100 9 866
Fragments arranged by size:
1350 866 100 9
BsiHKAI G_wGCw'C
Cuts at: 0 1046 1094 1349 2325
Size: 1046 48 255 976
Fragments arranged by size:
1046 976 255 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 58 208 2325
Size: 58 150 2117
Fragments arranged by size:
2117 150 58
Bsp1286I G_dGCh'C
Cuts at: 0 254 535 1046 1094 1349 1611 1778 1960
Size: 254 281 511 48 255 262 167 182
Cuts at: 1960 2325
Size: 365
Fragments arranged by size:
511 365 281 262 255 254 182 167
48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 504 777 1472 1999 2325
Size: 504 273 695 527 326
Fragments arranged by size:
695 527 504 326 273
BsrBI CCG'CTC
Cuts at: 0 324 2325
Size: 324 2001
BsrDI GCAATG_nn'
Cuts at: 0 1172 1419 2325
Size: 1172 247 906
Fragments arranged by size:
1172 906 247
BsrFI r'CCGG_y
Cuts at: 0 398 2325
Size: 398 1927
BssHII G'CGCG_C
Cuts at: 0 89 2325
Size: 89 2236
BssSI C'ACGA_G
Cuts at: 0 1697 2325
Size: 1697 628
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1516 2325
Size: 1516 809
BstEII G'GTnAC_C
Cuts at: 0 1986 2325
Size: 1986 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 700 1761 2325
Size: 700 1061 564
Fragments arranged by size:
1061 700 564
BstYI r'GATC_y
Cuts at: 0 158 239 1493 2106 2253 2325
Size: 158 81 1254 613 147 72
Fragments arranged by size:
1254 613 158 147 81 72
BstZ17I GTA'TAC
Cuts at: 0 1265 2325
Size: 1265 1060
Bsu36I CC'TnA_GG
Cuts at: 0 1586 1912 2325
Size: 1586 326 413
Fragments arranged by size:
1586 413 326
BtgI C'CryG_G
Cuts at: 0 1136 1540 1703 2249 2325
Size: 1136 404 163 546 76
Fragments arranged by size:
1136 546 404 163 76
BtsI GCAGTG_nn'
Cuts at: 0 1147 1323 1447 2325
Size: 1147 176 124 878
Fragments arranged by size:
1147 878 176 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 599 634 2325
Size: 599 35 1691
Fragments arranged by size:
1691 599 35
DraIII CAC_nnn'GTG
Cuts at: 0 2040 2166 2325
Size: 2040 126 159
Fragments arranged by size:
2040 159 126
DrdI GACnn_nn'nnGTC
Cuts at: 0 514 2325
Size: 514 1811
EaeI y'GGCC_r
Cuts at: 0 947 1452 2325
Size: 947 505 873
Fragments arranged by size:
947 873 505
EarI CTCTTCn'nnn_
Cuts at: 0 31 1398 2325
Size: 31 1367 927
Fragments arranged by size:
1367 927 31
EcoICRI GAG'CTC
Cuts at: 0 1044 1092 2325
Size: 1044 48 1233
Fragments arranged by size:
1233 1044 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 574 790 1143 1483 1735 2002 2030
Size: 490 84 216 353 340 252 267 28
Cuts at: 2030 2123 2323 2325
Size: 93 200 2
Fragments arranged by size:
490 353 340 267 252 216 200 93
84 28 2
EcoO109I rG'GnC_Cy
Cuts at: 0 516 532 681 1956 1957 2325
Size: 516 16 149 1275 1 368
Fragments arranged by size:
1275 516 368 149 16 1
EcoRI G'AATT_C
Cuts at: 0 1375 2325
Size: 1375 950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2298 2325
Size: 2298 27
FspI TGC'GCA
Cuts at: 0 1171 2325
Size: 1171 1154
HaeII r_GCGC'y
Cuts at: 0 266 1887 2325
Size: 266 1621 438
Fragments arranged by size:
1621 438 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 36 639 671 1116 1148 2325
Size: 4 32 603 32 445 32 1177
Fragments arranged by size:
1177 603 445 32 32 32 4
HindIII A'AGCT_T
Cuts at: 0 2275 2325
Size: 2275 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 2325
Size: 521 1804
MslI CAynn'nnrTG
Cuts at: 0 1708 1771 2325
Size: 1708 63 554
Fragments arranged by size:
1708 554 63
MspA1I CmG'CkG
Cuts at: 0 1789 2325
Size: 1789 536
NcoI C'CATG_G
Cuts at: 0 1136 1703 2325
Size: 1136 567 622
Fragments arranged by size:
1136 622 567
NdeI CA'TA_TG
Cuts at: 0 999 2325
Size: 999 1326
NspI r_CATG'y
Cuts at: 0 513 841 1948 2325
Size: 513 328 1107 377
Fragments arranged by size:
1107 513 377 328
PasI CC'CwG_GG
Cuts at: 0 129 2325
Size: 129 2196
PflMI CCAn_nnn'nTGG
Cuts at: 0 411 528 1905 2325
Size: 411 117 1377 420
Fragments arranged by size:
1377 420 411 117
PmlI CAC'GTG
Cuts at: 0 2163 2325
Size: 2163 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 2325
Size: 547 32 1746
Fragments arranged by size:
1746 547 32
PpuMI rG'GwC_Cy
Cuts at: 0 516 2325
Size: 516 1809
PspOMI G'GGCC_C
Cuts at: 0 531 1956 2325
Size: 531 1425 369
Fragments arranged by size:
1425 531 369
PstI C_TGCA'G
Cuts at: 0 1943 1996 2325
Size: 1943 53 329
Fragments arranged by size:
1943 329 53
PvuII CAG'CTG
Cuts at: 0 1789 2325
Size: 1789 536
SacI G_AGCT'C
Cuts at: 0 1046 1094 2325
Size: 1046 48 1231
Fragments arranged by size:
1231 1046 48
SapI GCTCTTCn'nnn_
Cuts at: 0 31 1398 2325
Size: 31 1367 927
Fragments arranged by size:
1367 927 31
SfcI C'TryA_G
Cuts at: 0 1244 1939 1992 2325
Size: 1244 695 53 333
Fragments arranged by size:
1244 695 333 53
SmaI CCC'GGG
Cuts at: 0 1612 2325
Size: 1612 713
SmlI C'TyrA_G
Cuts at: 0 70 271 685 2325
Size: 70 201 414 1640
Fragments arranged by size:
1640 414 201 70
SnaBI TAC'GTA
Cuts at: 0 793 2325
Size: 793 1532
StuI AGG'CCT
Cuts at: 0 2026 2325
Size: 2026 299
StyI C'CwwG_G
Cuts at: 0 293 872 1136 1370 1703 1760 2325
Size: 293 579 264 234 333 57 565
Fragments arranged by size:
579 565 333 293 264 234 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 328 1842 2028 2325
Size: 328 1514 186 297
Fragments arranged by size:
1514 328 297 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1279 1311 1610 1642 1728 1760 2325
Size: 1279 32 299 32 86 32 565
Fragments arranged by size:
1279 565 299 86 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 651 2325
Size: 651 1674
XbaI T'CTAG_A
Cuts at: 0 9 1656 2325
Size: 9 1647 669
Fragments arranged by size:
1647 669 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 309 957 1710 2325
Size: 309 648 753 615
Fragments arranged by size:
753 648 615 309
XmaI C'CCGG_G
Cuts at: 0 1610 2325
Size: 1610 715
XmnI GAAnn'nnTTC
Cuts at: 0 562 2325
Size: 562 1763
ZraI GAC'GTC
Cuts at: 0 1425 2325
Size: 1425 900
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflII AflIII AhdI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbsI BciVI BclI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HindIII MmeI MslI MspA1I NcoI NdeI NspI PasI
PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SapI SfcI SmaI SmlI SnaBI StuI StyI TaqII
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AgeI AleI AlfI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BcgI BfrBI BglI
BglII BmgBI BmtI BsaBI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BstBI BtgZI ClaI DraI EagI EciI
EcoNI EcoRV FseI FspAI HincII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SpeI SphI
SrfI SspI SwaI TatI XhoI