Restriction map for
pF1KB5066
(Linear) (Six-base) MAPSORT of: KIBB5066.seq Check: 7179 from: 1 to: 1725
KIBB5066.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 188 1117 1725
Size: 188 929 608
Fragments arranged by size:
929 608 188
AccI GT'mk_AC
Cuts at: 0 601 1725
Size: 601 1124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 233 362 864 1200 1725
Size: 46 187 129 502 336 525
Fragments arranged by size:
525 502 336 187 129 46
AhdI GACnn_n'nnGTC
Cuts at: 0 173 1725
Size: 173 1552
AlwNI CAG_nnn'CTG
Cuts at: 0 441 698 1290 1725
Size: 441 257 592 435
Fragments arranged by size:
592 441 435 257
ApaLI G'TGCA_C
Cuts at: 0 820 1657 1725
Size: 820 837 68
Fragments arranged by size:
837 820 68
ApoI r'AATT_y
Cuts at: 0 478 1725
Size: 478 1247
AvaI C'yCGr_G
Cuts at: 0 7 1515 1725
Size: 7 1508 210
Fragments arranged by size:
1508 210 7
BamHI G'GATC_C
Cuts at: 0 140 1725
Size: 140 1585
BanI G'GyrC_C
Cuts at: 0 1451 1725
Size: 1451 274
BanII G_rGCy'C
Cuts at: 0 1438 1570 1611 1725
Size: 1438 132 41 114
Fragments arranged by size:
1438 132 114 41
BglI GCCn_nnn'nGGC
Cuts at: 0 1488 1725
Size: 1488 237
BglII A'GATC_T
Cuts at: 0 578 1725
Size: 578 1147
Bme1580I G_kGCm'C
Cuts at: 0 824 1661 1725
Size: 824 837 64
Fragments arranged by size:
837 824 64
BmgBI CAC'GTC
Cuts at: 0 156 1725
Size: 156 1569
BmrI ACTGGGnnnn_n'
Cuts at: 0 1016 1100 1725
Size: 1016 84 625
Fragments arranged by size:
1016 625 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 250 624 944 1542 1725
Size: 184 66 374 320 598 183
Fragments arranged by size:
598 374 320 184 183 66
Bpu10I CC'TnA_GC
Cuts at: 0 197 1725
Size: 197 1528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 472 1725
Size: 433 39 1253
Fragments arranged by size:
1253 433 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 1725
Size: 160 1565
BsaHI Gr'CG_yC
Cuts at: 0 1306 1725
Size: 1306 419
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 375 405 583 613 1042 1072
Size: 169 30 176 30 178 30 429 30
Cuts at: 1072 1540 1570 1725
Size: 468 30 155
Fragments arranged by size:
468 429 178 176 169 155 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 882 1050 1192 1489 1725
Size: 361 521 168 142 297 236
Fragments arranged by size:
521 361 297 236 168 142
BseYI C'CCAG_C
Cuts at: 0 224 1238 1725
Size: 224 1014 487
Fragments arranged by size:
1014 487 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1141 1725
Size: 775 366 584
Fragments arranged by size:
775 584 366
BsiHKAI G_wGCw'C
Cuts at: 0 824 1438 1570 1661 1725
Size: 824 614 132 91 64
Fragments arranged by size:
824 614 132 91 64
Bsp1286I G_dGCh'C
Cuts at: 0 824 1438 1570 1611 1661 1725
Size: 824 614 132 41 50 64
Fragments arranged by size:
824 614 132 64 50 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 1117 1725
Size: 188 929 608
Fragments arranged by size:
929 608 188
BsrFI r'CCGG_y
Cuts at: 0 48 1496 1725
Size: 48 1448 229
Fragments arranged by size:
1448 229 48
BstEII G'GTnAC_C
Cuts at: 0 175 1725
Size: 175 1550
BstYI r'GATC_y
Cuts at: 0 140 578 1725
Size: 140 438 1147
Fragments arranged by size:
1147 438 140
BstZ17I GTA'TAC
Cuts at: 0 602 1725
Size: 602 1123
BtgI C'CryG_G
Cuts at: 0 1485 1725
Size: 1485 240
BtsI GCAGTG_nn'
Cuts at: 0 1119 1200 1725
Size: 1119 81 525
Fragments arranged by size:
1119 525 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 849 884 1725
Size: 849 35 841
Fragments arranged by size:
849 841 35
EarI CTCTTCn'nnn_
Cuts at: 0 323 338 1670 1725
Size: 323 15 1332 55
Fragments arranged by size:
1332 323 55 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1592 1725
Size: 1592 133
EcoICRI GAG'CTC
Cuts at: 0 1436 1568 1725
Size: 1436 132 157
Fragments arranged by size:
1436 157 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 184 233 250 362 624 864 944
Size: 46 138 49 17 112 262 240 80
Cuts at: 944 1200 1542 1725
Size: 256 342 183
Fragments arranged by size:
342 262 256 240 183 138 112 80
49 46 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1518 1725
Size: 1518 207
EcoO109I rG'GnC_Cy
Cuts at: 0 193 333 1043 1184 1725
Size: 193 140 710 141 541
Fragments arranged by size:
710 541 193 141 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 286 318 337 369 375 407
Size: 159 32 95 32 19 32 6 32
Cuts at: 407 423 455 1296 1328 1725
Size: 16 32 841 32 397
Fragments arranged by size:
841 397 159 95 32 32 32 32
32 32 19 16 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1725
Size: 455 1270
MspA1I CmG'CkG
Cuts at: 0 123 228 1248 1725
Size: 123 105 1020 477
Fragments arranged by size:
1020 477 123 105
NaeI GCC'GGC
Cuts at: 0 50 1498 1725
Size: 50 1448 227
Fragments arranged by size:
1448 227 50
NcoI C'CATG_G
Cuts at: 0 1485 1725
Size: 1485 240
NgoMIV G'CCGG_C
Cuts at: 0 48 1496 1725
Size: 48 1448 229
Fragments arranged by size:
1448 229 48
PasI CC'CwG_GG
Cuts at: 0 273 1065 1341 1725
Size: 273 792 276 384
Fragments arranged by size:
792 384 276 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 441 1725
Size: 441 1284
PfoI T'CCnGG_A
Cuts at: 0 80 1725
Size: 80 1645
PpuMI rG'GwC_Cy
Cuts at: 0 193 1725
Size: 193 1532
PspXI vC'TCGA_Gb
Cuts at: 0 1515 1725
Size: 1515 210
PstI C_TGCA'G
Cuts at: 0 1114 1575 1725
Size: 1114 461 150
Fragments arranged by size:
1114 461 150
PvuII CAG'CTG
Cuts at: 0 123 228 1725
Size: 123 105 1497
Fragments arranged by size:
1497 123 105
SacI G_AGCT'C
Cuts at: 0 1438 1570 1725
Size: 1438 132 155
Fragments arranged by size:
1438 155 132
SapI GCTCTTCn'nnn_
Cuts at: 0 1670 1725
Size: 1670 55
SexAI A'CCwGG_T
Cuts at: 0 1532 1725
Size: 1532 193
SfcI C'TryA_G
Cuts at: 0 1110 1571 1725
Size: 1110 461 154
Fragments arranged by size:
1110 461 154
SgrAI Cr'CCGG_yG
Cuts at: 0 48 1725
Size: 48 1677
SmlI C'TyrA_G
Cuts at: 0 448 487 1515 1725
Size: 448 39 1028 210
Fragments arranged by size:
1028 448 210 39
StyI C'CwwG_G
Cuts at: 0 1485 1725
Size: 1485 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 240 256 286 1678 1725
Size: 240 16 30 1392 47
Fragments arranged by size:
1392 240 47 30 16
TatI w'GTAC_w
Cuts at: 0 775 973 1725
Size: 775 198 752
Fragments arranged by size:
775 752 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 694 726 1213 1245 1540 1572
Size: 169 32 493 32 487 32 295 32
Cuts at: 1572 1725
Size: 153
Fragments arranged by size:
493 487 295 169 153 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 583 1725
Size: 583 1142
XhoI C'TCGA_G
Cuts at: 0 1515 1725
Size: 1515 210
XmnI GAAnn'nnTTC
Cuts at: 0 877 961 1129 1213 1423 1725
Size: 877 84 168 84 210 302
Fragments arranged by size:
877 302 210 168 84 84
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BglI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BstEII BstYI BstZ17I
BtgI BtsI CspCI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I Hin4I MmeI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PpuMI PspXI PstI PvuII SacI SapI
SexAI SfcI SgrAI SmlI StyI TaqII TatI TstI
XbaI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstXI Bsu36I BtgZI ClaI DraI DraIII
DrdI EaeI EagI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XcmI XmaI
ZraI