Restriction map for
pF1KB5072
(Linear) (Six-base) MAPSORT of: KIBB5072.seq Check: 4603 from: 1 to: 1848
KIBB5072.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 950 1848
Size: 950 898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 805 1628 1848
Size: 335 470 823 220
Fragments arranged by size:
823 470 335 220
AleI CACnn'nnGTG
Cuts at: 0 1402 1848
Size: 1402 446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 361 1848
Size: 361 1487
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 1848
Size: 142 32 1674
Fragments arranged by size:
1674 142 32
AlwNI CAG_nnn'CTG
Cuts at: 0 221 1848
Size: 221 1627
ApoI r'AATT_y
Cuts at: 0 1099 1516 1848
Size: 1099 417 332
Fragments arranged by size:
1099 417 332
AvaI C'yCGr_G
Cuts at: 0 364 444 644 922 1142 1848
Size: 364 80 200 278 220 706
Fragments arranged by size:
706 364 278 220 200 80
BanI G'GyrC_C
Cuts at: 0 552 778 1848
Size: 552 226 1070
Fragments arranged by size:
1070 552 226
BanII G_rGCy'C
Cuts at: 0 1143 1848
Size: 1143 705
BbsI GAAGACnn'nnnn_
Cuts at: 0 986 1175 1848
Size: 986 189 673
Fragments arranged by size:
986 673 189
BciVI GTATCCnnnnn_n'
Cuts at: 0 45 1848
Size: 45 1803
BclI T'GATC_A
Cuts at: 0 1473 1848
Size: 1473 375
BglI GCCn_nnn'nGGC
Cuts at: 0 31 1848
Size: 31 1817
BlpI GC'TnA_GC
Cuts at: 0 13 1848
Size: 13 1835
Bme1580I G_kGCm'C
Cuts at: 0 350 557 1848
Size: 350 207 1291
Fragments arranged by size:
1291 350 207
BmgBI CAC'GTC
Cuts at: 0 1779 1848
Size: 1779 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 654 1848
Size: 371 283 1194
Fragments arranged by size:
1194 371 283
BsaI GGTCTCn'nnnn_
Cuts at: 0 1729 1848
Size: 1729 119
BsaHI Gr'CG_yC
Cuts at: 0 983 1848
Size: 983 865
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1130 1160 1848
Size: 1130 30 688
Fragments arranged by size:
1130 688 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 1848
Size: 374 1474
BseYI C'CCAG_C
Cuts at: 0 405 425 511 1848
Size: 405 20 86 1337
Fragments arranged by size:
1337 405 86 20
BsiHKAI G_wGCw'C
Cuts at: 0 603 1848
Size: 603 1245
BsmI GAATG_Cn'
Cuts at: 0 1695 1848
Size: 1695 153
Bsp1286I G_dGCh'C
Cuts at: 0 350 557 603 1143 1848
Size: 350 207 46 540 705
Fragments arranged by size:
705 540 350 207 46
BspHI T'CATG_A
Cuts at: 0 134 1820 1848
Size: 134 1686 28
Fragments arranged by size:
1686 134 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 496 1848
Size: 496 1352
BsrBI CCG'CTC
Cuts at: 0 1295 1848
Size: 1295 553
BsrDI GCAATG_nn'
Cuts at: 0 26 557 1196 1848
Size: 26 531 639 652
Fragments arranged by size:
652 639 531 26
BssSI C'ACGA_G
Cuts at: 0 193 602 1848
Size: 193 409 1246
Fragments arranged by size:
1246 409 193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1773 1848
Size: 1773 75
BstYI r'GATC_y
Cuts at: 0 623 962 1848
Size: 623 339 886
Fragments arranged by size:
886 623 339
BtgI C'CryG_G
Cuts at: 0 1787 1848
Size: 1787 61
EaeI y'GGCC_r
Cuts at: 0 6 32 1394 1806 1848
Size: 6 26 1362 412 42
Fragments arranged by size:
1362 412 42 26 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 1313 1848
Size: 104 1209 535
Fragments arranged by size:
1209 535 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 371 654 805 1628 1848
Size: 335 36 283 151 823 220
Fragments arranged by size:
823 335 283 220 151 36
EcoO109I rG'GnC_Cy
Cuts at: 0 422 508 1848
Size: 422 86 1340
Fragments arranged by size:
1340 422 86
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 676 961 1633 1848
Size: 676 285 672 215
Fragments arranged by size:
676 672 285 215
HaeII r_GCGC'y
Cuts at: 0 394 1848
Size: 394 1454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 324 356 916 948 1130 1154
Size: 266 32 26 32 560 32 182 24
Cuts at: 1154 1162 1186 1330 1362 1709 1741 1848
Size: 8 24 144 32 347 32 107
Fragments arranged by size:
560 347 266 182 144 107 32 32
32 32 32 26 24 24 8
HincII GTy'rAC
Cuts at: 0 681 1848
Size: 681 1167
HindIII A'AGCT_T
Cuts at: 0 658 1228 1848
Size: 658 570 620
Fragments arranged by size:
658 620 570
MfeI C'AATT_G
Cuts at: 0 208 1848
Size: 208 1640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 1284 1848
Size: 303 981 564
Fragments arranged by size:
981 564 303
MscI TGG'CCA
Cuts at: 0 1396 1848
Size: 1396 452
MslI CAynn'nnrTG
Cuts at: 0 1402 1771 1848
Size: 1402 369 77
Fragments arranged by size:
1402 369 77
MspA1I CmG'CkG
Cuts at: 0 12 313 811 1496 1848
Size: 12 301 498 685 352
Fragments arranged by size:
685 498 352 301 12
NspI r_CATG'y
Cuts at: 0 1431 1848
Size: 1431 417
PfoI T'CCnGG_A
Cuts at: 0 1054 1595 1848
Size: 1054 541 253
Fragments arranged by size:
1054 541 253
PpuMI rG'GwC_Cy
Cuts at: 0 508 1848
Size: 508 1340
PshAI GACnn'nnGTC
Cuts at: 0 853 1848
Size: 853 995
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1414 1446 1848
Size: 1414 32 402
Fragments arranged by size:
1414 402 32
PstI C_TGCA'G
Cuts at: 0 507 1848
Size: 507 1341
PvuII CAG'CTG
Cuts at: 0 12 313 811 1848
Size: 12 301 498 1037
Fragments arranged by size:
1037 498 301 12
SfcI C'TryA_G
Cuts at: 0 503 704 1794 1848
Size: 503 201 1090 54
Fragments arranged by size:
1090 503 201 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 31 1848
Size: 31 1817
SmlI C'TyrA_G
Cuts at: 0 922 1848
Size: 922 926
SspI AAT'ATT
Cuts at: 0 1557 1848
Size: 1557 291
StuI AGG'CCT
Cuts at: 0 1222 1848
Size: 1222 626
StyI C'CwwG_G
Cuts at: 0 1362 1617 1848
Size: 1362 255 231
Fragments arranged by size:
1362 255 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1572 1848
Size: 1572 276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 582 614 1503 1535 1848
Size: 582 32 889 32 313
Fragments arranged by size:
889 582 313 32 32
XbaI T'CTAG_A
Cuts at: 0 1111 1848
Size: 1111 737
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 433 1848
Size: 433 1415
XhoI C'TCGA_G
Cuts at: 0 922 1848
Size: 922 926
XmnI GAAnn'nnTTC
Cuts at: 0 453 661 1848
Size: 453 208 1187
Fragments arranged by size:
1187 453 208
Enzymes that do cut:
AccI AcuI AleI AlfI AloI AlwNI ApoI AvaI
BanI BanII BbsI BciVI BclI BglI BlpI Bme1580I
BmgBI BpmI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstXI
BstYI BtgI EaeI EciI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NspI PfoI PpuMI PshAI PsrI PstI PvuII SfcI
SfiI SmlI SspI StuI StyI TaqII TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BglII BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TatI Tth111I XmaI
ZraI