Restriction map for
pF1KB5078
(Linear) (Six-base) MAPSORT of: KIBB5078.seq Check: 2261 from: 1 to: 2571
KIBB5078.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 45 1626 2406 2571
Size: 45 1581 780 165
Fragments arranged by size:
1581 780 165 45
AccI GT'mk_AC
Cuts at: 0 332 2571
Size: 332 2239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 931 2030 2571
Size: 808 123 1099 541
Fragments arranged by size:
1099 808 541 123
AfeI AGC'GCT
Cuts at: 0 1045 1741 2571
Size: 1045 696 830
Fragments arranged by size:
1045 830 696
AflIII A'CryG_T
Cuts at: 0 152 1593 2571
Size: 152 1441 978
Fragments arranged by size:
1441 978 152
AleI CACnn'nnGTG
Cuts at: 0 520 1831 2571
Size: 520 1311 740
Fragments arranged by size:
1311 740 520
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 2571
Size: 1489 32 1050
Fragments arranged by size:
1489 1050 32
AlwNI CAG_nnn'CTG
Cuts at: 0 658 1671 2337 2398 2517 2571
Size: 658 1013 666 61 119 54
Fragments arranged by size:
1013 666 658 119 61 54
ApaLI G'TGCA_C
Cuts at: 0 909 2571
Size: 909 1662
ApoI r'AATT_y
Cuts at: 0 1180 2571
Size: 1180 1391
AvaI C'yCGr_G
Cuts at: 0 15 2571
Size: 15 2556
BanI G'GyrC_C
Cuts at: 0 66 494 1236 2004 2266 2571
Size: 66 428 742 768 262 305
Fragments arranged by size:
768 742 428 305 262 66
BanII G_rGCy'C
Cuts at: 0 1940 2330 2571
Size: 1940 390 241
Fragments arranged by size:
1940 390 241
BbeI G_GCGC'C
Cuts at: 0 1240 2571
Size: 1240 1331
BbsI GAAGACnn'nnnn_
Cuts at: 0 1573 1919 2571
Size: 1573 346 652
Fragments arranged by size:
1573 652 346
BbvCI CC'TCA_GC
Cuts at: 0 1583 2571
Size: 1583 988
BciVI GTATCCnnnnn_n'
Cuts at: 0 968 2538 2571
Size: 968 1570 33
Fragments arranged by size:
1570 968 33
BglI GCCn_nnn'nGGC
Cuts at: 0 80 2255 2571
Size: 80 2175 316
Fragments arranged by size:
2175 316 80
BglII A'GATC_T
Cuts at: 0 248 2571
Size: 248 2323
Bme1580I G_kGCm'C
Cuts at: 0 913 2269 2433 2571
Size: 913 1356 164 138
Fragments arranged by size:
1356 913 164 138
BmgBI CAC'GTC
Cuts at: 0 153 2571
Size: 153 2418
BmtI G_CTAG'C
Cuts at: 0 400 863 2571
Size: 400 463 1708
Fragments arranged by size:
1708 463 400
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1624 2571
Size: 1624 947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1094 2314 2571
Size: 907 187 1220 257
Fragments arranged by size:
1220 907 257 187
Bpu10I CC'TnA_GC
Cuts at: 0 1583 2571
Size: 1583 988
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1378 1501 1672 2571
Size: 937 441 123 171 899
Fragments arranged by size:
937 899 441 171 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 387 2571
Size: 387 2184
BsaAI yAC'GTr
Cuts at: 0 2364 2571
Size: 2364 207
BsaBI GATnn'nnATC
Cuts at: 0 1333 1444 2571
Size: 1333 111 1127
Fragments arranged by size:
1333 1127 111
BsaHI Gr'CG_yC
Cuts at: 0 1237 1570 2571
Size: 1237 333 1001
Fragments arranged by size:
1237 1001 333
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1489 1519 1769 1799 2571
Size: 1489 30 250 30 772
Fragments arranged by size:
1489 772 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1138 1930 2571
Size: 1138 792 641
Fragments arranged by size:
1138 792 641
BseYI C'CCAG_C
Cuts at: 0 567 1036 1797 1858 2184 2188 2391 2571
Size: 567 469 761 61 326 4 203 180
Fragments arranged by size:
761 567 469 326 203 180 61 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 2134 2265 2551 2571
Size: 1654 480 131 286 20
Fragments arranged by size:
1654 480 286 131 20
BsiEI CG_ry'CG
Cuts at: 0 1260 2571
Size: 1260 1311
BsiHKAI G_wGCw'C
Cuts at: 0 913 1394 1940 2330 2571
Size: 913 481 546 390 241
Fragments arranged by size:
913 546 481 390 241
BsiWI C'GTAC_G
Cuts at: 0 2364 2571
Size: 2364 207
BsmI GAATG_Cn'
Cuts at: 0 422 1152 2571
Size: 422 730 1419
Fragments arranged by size:
1419 730 422
Bsp1286I G_dGCh'C
Cuts at: 0 913 1394 1940 2269 2330 2433 2571
Size: 913 481 546 329 61 103 138
Fragments arranged by size:
913 546 481 329 138 103 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 47 1626 2406 2523 2571
Size: 45 2 1579 780 117 48
Fragments arranged by size:
1579 780 117 48 45 2
BsrBI CCG'CTC
Cuts at: 0 530 1108 2571
Size: 530 578 1463
Fragments arranged by size:
1463 578 530
BsrDI GCAATG_nn'
Cuts at: 0 1271 2502 2571
Size: 1271 1231 69
Fragments arranged by size:
1271 1231 69
BsrFI r'CCGG_y
Cuts at: 0 74 1233 1572 2571
Size: 74 1159 339 999
Fragments arranged by size:
1159 999 339 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1632 2571
Size: 1632 939
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1183 2571
Size: 1183 1388
BstYI r'GATC_y
Cuts at: 0 248 446 1102 1164 2333 2571
Size: 248 198 656 62 1169 238
Fragments arranged by size:
1169 656 248 238 198 62
Bsu36I CC'TnA_GG
Cuts at: 0 548 2571
Size: 548 2023
BtgI C'CryG_G
Cuts at: 0 1894 2571
Size: 1894 677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1335 2571
Size: 1335 1236
BtsI GCAGTG_nn'
Cuts at: 0 307 656 1633 1838 2571
Size: 307 349 977 205 733
Fragments arranged by size:
977 733 349 307 205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1689 1724 2571
Size: 1689 35 847
Fragments arranged by size:
1689 847 35
DraIII CAC_nnn'GTG
Cuts at: 0 2445 2571
Size: 2445 126
EaeI y'GGCC_r
Cuts at: 0 116 441 485 984 1257 2048 2519 2571
Size: 116 325 44 499 273 791 471 52
Fragments arranged by size:
791 499 471 325 273 116 52 44
EagI C'GGCC_G
Cuts at: 0 1257 2571
Size: 1257 1314
EarI CTCTTCn'nnn_
Cuts at: 0 814 1379 2164 2571
Size: 814 565 785 407
Fragments arranged by size:
814 785 565 407
EcoICRI GAG'CTC
Cuts at: 0 1938 2328 2571
Size: 1938 390 243
Fragments arranged by size:
1938 390 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 907 931 1094 2030 2314 2571
Size: 808 99 24 163 936 284 257
Fragments arranged by size:
936 808 284 257 163 99 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 356 2571
Size: 52 304 2215
Fragments arranged by size:
2215 304 52
EcoO109I rG'GnC_Cy
Cuts at: 0 10 429 783 2505 2514 2571
Size: 10 419 354 1722 9 57
Fragments arranged by size:
1722 419 354 57 10 9
FspI TGC'GCA
Cuts at: 0 2554 2571
Size: 2554 17
HaeII r_GCGC'y
Cuts at: 0 35 1047 1240 1743 2571
Size: 35 1012 193 503 828
Fragments arranged by size:
1012 828 503 193 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1647 1679 2571
Size: 1647 32 892
Fragments arranged by size:
1647 892 32
HincII GTy'rAC
Cuts at: 0 204 333 1456 2571
Size: 204 129 1123 1115
Fragments arranged by size:
1123 1115 204 129
KasI G'GCGC_C
Cuts at: 0 1236 2571
Size: 1236 1335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1578 1700 2571
Size: 1578 122 871
Fragments arranged by size:
1578 871 122
MscI TGG'CCA
Cuts at: 0 118 487 986 2050 2521 2571
Size: 118 369 499 1064 471 50
Fragments arranged by size:
1064 499 471 369 118 50
MslI CAynn'nnrTG
Cuts at: 0 520 1831 2288 2462 2571
Size: 520 1311 457 174 109
Fragments arranged by size:
1311 520 457 174 109
MspA1I CmG'CkG
Cuts at: 0 25 440 567 885 1413 2188 2349 2571
Size: 25 415 127 318 528 775 161 222
Fragments arranged by size:
775 528 415 318 222 161 127 25
NaeI GCC'GGC
Cuts at: 0 76 1235 2571
Size: 76 1159 1336
Fragments arranged by size:
1336 1159 76
NarI GG'CG_CC
Cuts at: 0 1237 2571
Size: 1237 1334
NgoMIV G'CCGG_C
Cuts at: 0 74 1233 2571
Size: 74 1159 1338
Fragments arranged by size:
1338 1159 74
NheI G'CTAG_C
Cuts at: 0 396 859 2571
Size: 396 463 1712
Fragments arranged by size:
1712 463 396
NspI r_CATG'y
Cuts at: 0 1305 2571
Size: 1305 1266
PasI CC'CwG_GG
Cuts at: 0 352 2571
Size: 352 2219
PflMI CCAn_nnn'nTGG
Cuts at: 0 392 567 2445 2517 2571
Size: 392 175 1878 72 54
Fragments arranged by size:
1878 392 175 72 54
PfoI T'CCnGG_A
Cuts at: 0 1175 2571
Size: 1175 1396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 1489 1513 1521 1545 2571
Size: 574 32 883 24 8 24 1026
Fragments arranged by size:
1026 883 574 32 24 24 8
PpuMI rG'GwC_Cy
Cuts at: 0 10 2505 2571
Size: 10 2495 66
Fragments arranged by size:
2495 66 10
PsiI TTA'TAA
Cuts at: 0 668 2571
Size: 668 1903
PstI C_TGCA'G
Cuts at: 0 53 1541 2571
Size: 53 1488 1030
Fragments arranged by size:
1488 1030 53
PvuII CAG'CTG
Cuts at: 0 25 885 1413 2188 2349 2571
Size: 25 860 528 775 161 222
Fragments arranged by size:
860 775 528 222 161 25
SacI G_AGCT'C
Cuts at: 0 1940 2330 2571
Size: 1940 390 241
Fragments arranged by size:
1940 390 241
SalI G'TCGA_C
Cuts at: 0 331 2571
Size: 331 2240
SanDI GG'GwC_CC
Cuts at: 0 10 2505 2571
Size: 10 2495 66
Fragments arranged by size:
2495 66 10
SexAI A'CCwGG_T
Cuts at: 0 2441 2571
Size: 2441 130
SfcI C'TryA_G
Cuts at: 0 49 1537 2571
Size: 49 1488 1034
Fragments arranged by size:
1488 1034 49
SfoI GGC'GCC
Cuts at: 0 1238 2571
Size: 1238 1333
SgrAI Cr'CCGG_yG
Cuts at: 0 1572 2571
Size: 1572 999
SmlI C'TyrA_G
Cuts at: 0 952 1393 1516 1687 2571
Size: 952 441 123 171 884
Fragments arranged by size:
952 884 441 171 123
SnaBI TAC'GTA
Cuts at: 0 2364 2571
Size: 2364 207
StyI C'CwwG_G
Cuts at: 0 458 770 1427 2571
Size: 458 312 657 1144
Fragments arranged by size:
1144 657 458 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 817 1078 1296 2230 2234 2571
Size: 817 261 218 934 4 337
Fragments arranged by size:
934 817 337 261 218 4
TatI w'GTAC_w
Cuts at: 0 2561 2571
Size: 2561 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 487 519 887 919 2571
Size: 487 32 368 32 1652
Fragments arranged by size:
1652 487 368 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1671 2571
Size: 1671 900
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 2571
Size: 495 2076
XmnI GAAnn'nnTTC
Cuts at: 0 1315 2571
Size: 1315 1256
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AloI AlwNI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BciVI BglI BglII Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PflMI PfoI PpiI PpuMI PsiI PstI
PvuII SacI SalI SanDI SexAI SfcI SfoI SgrAI
SmlI SnaBI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BclI
BfrBI BlpI BmrI BsaWI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstBI BstEII BstZ17I ClaI DraI DrdI
EciI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SapI SbfI ScaI SfiI SgfI
SmaI SpeI SphI SrfI SspI StuI SwaI XbaI
XhoI XmaI ZraI