Restriction map for
pF1KB5081
(Linear) (Six-base) MAPSORT of: KIBB5081.seq Check: 8642 from: 1 to: 1623
KIBB5081.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 1349 1388 1623
Size: 1349 39 235
Fragments arranged by size:
1349 235 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1623
Size: 770 853
AfeI AGC'GCT
Cuts at: 0 253 1623
Size: 253 1370
AgeI A'CCGG_T
Cuts at: 0 1391 1623
Size: 1391 232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 553 1623
Size: 553 1070
AlwNI CAG_nnn'CTG
Cuts at: 0 1486 1623
Size: 1486 137
ApaI G_GGCC'C
Cuts at: 0 247 1580 1623
Size: 247 1333 43
Fragments arranged by size:
1333 247 43
AvaI C'yCGr_G
Cuts at: 0 17 122 273 1623
Size: 17 105 151 1350
Fragments arranged by size:
1350 151 105 17
BamHI G'GATC_C
Cuts at: 0 1521 1623
Size: 1521 102
BanI G'GyrC_C
Cuts at: 0 343 382 466 657 1144 1165 1623
Size: 343 39 84 191 487 21 458
Fragments arranged by size:
487 458 343 191 84 39 21
BanII G_rGCy'C
Cuts at: 0 35 50 247 953 1580 1623
Size: 35 15 197 706 627 43
Fragments arranged by size:
706 627 197 43 35 15
BbeI G_GCGC'C
Cuts at: 0 347 386 470 1148 1623
Size: 347 39 84 678 475
Fragments arranged by size:
678 475 347 84 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 1623
Size: 261 1362
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 318 352 1623
Size: 318 34 1271
Fragments arranged by size:
1271 318 34
BclI T'GATC_A
Cuts at: 0 305 1623
Size: 305 1318
BglI GCCn_nnn'nGGC
Cuts at: 0 187 196 380 666 1623
Size: 187 9 184 286 957
Fragments arranged by size:
957 286 187 184 9
BlpI GC'TnA_GC
Cuts at: 0 1210 1623
Size: 1210 413
Bme1580I G_kGCm'C
Cuts at: 0 247 662 1580 1623
Size: 247 415 918 43
Fragments arranged by size:
918 415 247 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 217 1623
Size: 217 1406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1324 1623
Size: 1324 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1623
Size: 1319 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 1623
Size: 276 1347
BsaI GGTCTCn'nnnn_
Cuts at: 0 813 1623
Size: 813 810
BsaAI yAC'GTr
Cuts at: 0 1455 1623
Size: 1455 168
BsaBI GATnn'nnATC
Cuts at: 0 307 688 1623
Size: 307 381 935
Fragments arranged by size:
935 381 307
BsaHI Gr'CG_yC
Cuts at: 0 344 383 424 467 1024 1145 1623
Size: 344 39 41 43 557 121 478
Fragments arranged by size:
557 478 344 121 43 41 39
BsaWI w'CCGG_w
Cuts at: 0 640 1391 1623
Size: 640 751 232
Fragments arranged by size:
751 640 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1342 1372 1623
Size: 1342 30 251
Fragments arranged by size:
1342 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 5 1108 1432 1549 1623
Size: 5 1103 324 117 74
Fragments arranged by size:
1103 324 117 74 5
BseYI C'CCAG_C
Cuts at: 0 201 526 741 930 1623
Size: 201 325 215 189 693
Fragments arranged by size:
693 325 215 201 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 1623
Size: 348 1275
BsiEI CG_ry'CG
Cuts at: 0 375 1349 1392 1598 1623
Size: 375 974 43 206 25
Fragments arranged by size:
974 375 206 43 25
BsiHKAI G_wGCw'C
Cuts at: 0 542 1623
Size: 542 1081
BsiWI C'GTAC_G
Cuts at: 0 1462 1623
Size: 1462 161
Bsp1286I G_dGCh'C
Cuts at: 0 35 50 247 542 662 953 1580 1623
Size: 35 15 197 295 120 291 627 43
Fragments arranged by size:
627 295 291 197 120 43 35 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 1623
Size: 164 1459
BsrDI GCAATG_nn'
Cuts at: 0 1199 1623
Size: 1199 424
BsrFI r'CCGG_y
Cuts at: 0 190 1391 1623
Size: 190 1201 232
Fragments arranged by size:
1201 232 190
BssHII G'CGCG_C
Cuts at: 0 365 1623
Size: 365 1258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1588 1623
Size: 1588 35
BstEII G'GTnAC_C
Cuts at: 0 408 1623
Size: 408 1215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 804 1623
Size: 420 384 819
Fragments arranged by size:
819 420 384
BstYI r'GATC_y
Cuts at: 0 555 1521 1623
Size: 555 966 102
Fragments arranged by size:
966 555 102
BtgI C'CryG_G
Cuts at: 0 182 494 503 662 797 945 1592 1623
Size: 182 312 9 159 135 148 647 31
Fragments arranged by size:
647 312 182 159 148 135 31 9
BtsI GCAGTG_nn'
Cuts at: 0 516 1034 1199 1623
Size: 516 518 165 424
Fragments arranged by size:
518 516 424 165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1023 1058 1623
Size: 1023 35 565
Fragments arranged by size:
1023 565 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1384 1623
Size: 1384 239
EaeI y'GGCC_r
Cuts at: 0 5 179 188 372 1031 1187 1259 1316
Size: 5 174 9 184 659 156 72 57
Cuts at: 1316 1623
Size: 307
Fragments arranged by size:
659 307 184 174 156 72 57 9
5
EagI C'GGCC_G
Cuts at: 0 372 1623
Size: 372 1251
EarI CTCTTCn'nnn_
Cuts at: 0 620 985 1056 1180 1498 1623
Size: 620 365 71 124 318 125
Fragments arranged by size:
620 365 318 125 124 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 465 1050 1623
Size: 58 407 585 573
Fragments arranged by size:
585 573 407 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1319 1623
Size: 770 549 304
Fragments arranged by size:
770 549 304
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1298 1623
Size: 1298 325
EcoO109I rG'GnC_Cy
Cuts at: 0 63 745 1046 1576 1623
Size: 63 682 301 530 47
Fragments arranged by size:
682 530 301 63 47
HaeII r_GCGC'y
Cuts at: 0 73 202 255 263 347 386 394 433
Size: 73 129 53 8 84 39 8 39
Cuts at: 433 451 470 733 1148 1623
Size: 18 19 263 415 475
Fragments arranged by size:
475 415 263 129 84 73 53 39
39 19 18 8 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 1597 1623
Size: 1342 32 223 26
Fragments arranged by size:
1342 223 32 26
HincII GTy'rAC
Cuts at: 0 1155 1338 1350 1389 1623
Size: 1155 183 12 39 234
Fragments arranged by size:
1155 234 183 39 12
KasI G'GCGC_C
Cuts at: 0 343 382 466 1144 1623
Size: 343 39 84 678 479
Fragments arranged by size:
678 479 343 84 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1623
Size: 605 1018
MscI TGG'CCA
Cuts at: 0 1033 1318 1623
Size: 1033 285 305
Fragments arranged by size:
1033 305 285
MspA1I CmG'CkG
Cuts at: 0 40 140 318 505 1029 1074 1285 1527
Size: 40 100 178 187 524 45 211 242
Cuts at: 1527 1623
Size: 96
Fragments arranged by size:
524 242 211 187 178 100 96 45
40
NaeI GCC'GGC
Cuts at: 0 192 1623
Size: 192 1431
NarI GG'CG_CC
Cuts at: 0 344 383 467 1145 1623
Size: 344 39 84 678 478
Fragments arranged by size:
678 478 344 84 39
NcoI C'CATG_G
Cuts at: 0 797 945 1623
Size: 797 148 678
Fragments arranged by size:
797 678 148
NgoMIV G'CCGG_C
Cuts at: 0 190 1623
Size: 190 1433
PflMI CCAn_nnn'nTGG
Cuts at: 0 1185 1623
Size: 1185 438
PfoI T'CCnGG_A
Cuts at: 0 551 811 1623
Size: 551 260 812
Fragments arranged by size:
812 551 260
PpuMI rG'GwC_Cy
Cuts at: 0 1046 1623
Size: 1046 577
PspOMI G'GGCC_C
Cuts at: 0 243 1576 1623
Size: 243 1333 47
Fragments arranged by size:
1333 243 47
PstI C_TGCA'G
Cuts at: 0 461 1623
Size: 461 1162
SacII CC_GC'GG
Cuts at: 0 506 1623
Size: 506 1117
SalI G'TCGA_C
Cuts at: 0 1348 1387 1623
Size: 1348 39 236
Fragments arranged by size:
1348 236 39
SapI GCTCTTCn'nnn_
Cuts at: 0 1180 1623
Size: 1180 443
SexAI A'CCwGG_T
Cuts at: 0 128 584 1623
Size: 128 456 1039
Fragments arranged by size:
1039 456 128
SfcI C'TryA_G
Cuts at: 0 457 1623
Size: 457 1166
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 187 1623
Size: 187 1436
SfoI GGC'GCC
Cuts at: 0 345 384 468 1146 1623
Size: 345 39 84 678 477
Fragments arranged by size:
678 477 345 84 39
SmaI CCC'GGG
Cuts at: 0 124 275 1623
Size: 124 151 1348
Fragments arranged by size:
1348 151 124
SmlI C'TyrA_G
Cuts at: 0 17 255 1623
Size: 17 238 1368
Fragments arranged by size:
1368 238 17
StyI C'CwwG_G
Cuts at: 0 26 90 797 903 945 1223 1623
Size: 26 64 707 106 42 278 400
Fragments arranged by size:
707 400 278 106 64 42 26
TatI w'GTAC_w
Cuts at: 0 911 1623
Size: 911 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 197 229 635 667 1623
Size: 197 32 406 32 956
Fragments arranged by size:
956 406 197 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 63 801 1155 1623
Size: 63 738 354 468
Fragments arranged by size:
738 468 354 63
XhoI C'TCGA_G
Cuts at: 0 17 1623
Size: 17 1606
XmaI C'CCGG_G
Cuts at: 0 122 273 1623
Size: 122 151 1350
Fragments arranged by size:
1350 151 122
Enzymes that do cut:
AccI AcuI AfeI AgeI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BcgI BclI BglI
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspMI BsrDI BsrFI BssHII BstAPI
BstEII BstXI BstYI BtgI BtsI CspCI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI MmeI MscI MspA1I NaeI NarI NcoI
NgoMIV PflMI PfoI PpuMI PspOMI PstI SacII SalI
SapI SexAI SfcI SfiI SfoI SmaI SmlI StyI
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BfrBI BglII BmgBI BmtI Bpu10I BsmI
BsmBI BspEI BspHI BsrBI BsrGI BssSI BstBI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MslI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SanDI SbfI
ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XmnI ZraI