Restriction map for
pF1KB5086
(Linear) (Six-base) MAPSORT of: KIBB5086.seq Check: 9401 from: 1 to: 1980
KIBB5086.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 1750 1980
Size: 1750 230
AclI AA'CG_TT
Cuts at: 0 1073 1980
Size: 1073 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 958 979 1017 1120 1387 1980
Size: 958 21 38 103 267 593
Fragments arranged by size:
958 593 267 103 38 21
AfeI AGC'GCT
Cuts at: 0 1381 1980
Size: 1381 599
AflIII A'CryG_T
Cuts at: 0 905 1980
Size: 905 1075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1336 1880 1980
Size: 1336 544 100
Fragments arranged by size:
1336 544 100
AlwNI CAG_nnn'CTG
Cuts at: 0 954 1641 1976 1980
Size: 954 687 335 4
Fragments arranged by size:
954 687 335 4
ApaI G_GGCC'C
Cuts at: 0 1264 1765 1792 1980
Size: 1264 501 27 188
Fragments arranged by size:
1264 501 188 27
ApaLI G'TGCA_C
Cuts at: 0 1510 1980
Size: 1510 470
AvaI C'yCGr_G
Cuts at: 0 34 48 1188 1980
Size: 34 14 1140 792
Fragments arranged by size:
1140 792 34 14
BanI G'GyrC_C
Cuts at: 0 1675 1980
Size: 1675 305
BanII G_rGCy'C
Cuts at: 0 41 181 1264 1765 1792 1980
Size: 41 140 1083 501 27 188
Fragments arranged by size:
1083 501 188 140 41 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 756 1980
Size: 756 1224
BbvCI CC'TCA_GC
Cuts at: 0 1886 1980
Size: 1886 94
BclI T'GATC_A
Cuts at: 0 1232 1980
Size: 1232 748
BglI GCCn_nnn'nGGC
Cuts at: 0 70 668 1980
Size: 70 598 1312
Fragments arranged by size:
1312 598 70
BglII A'GATC_T
Cuts at: 0 689 1064 1092 1980
Size: 689 375 28 888
Fragments arranged by size:
888 689 375 28
Bme1580I G_kGCm'C
Cuts at: 0 1054 1264 1514 1765 1792 1980
Size: 1054 210 250 251 27 188
Fragments arranged by size:
1054 251 250 210 188 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1583 1980
Size: 1583 397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 1980
Size: 60 1920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 697 1678 1847 1864 1980
Size: 469 228 981 169 17 116
Fragments arranged by size:
981 469 228 169 116 17
Bpu10I CC'TnA_GC
Cuts at: 0 1886 1892 1980
Size: 1886 6 88
Fragments arranged by size:
1886 88 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 241 1980
Size: 241 1739
BsaWI w'CCGG_w
Cuts at: 0 419 1391 1980
Size: 419 972 589
Fragments arranged by size:
972 589 419
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1105 1135 1165 1195 1980
Size: 1105 30 30 30 785
Fragments arranged by size:
1105 785 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1108 1159 1162 1681 1830 1980
Size: 1108 51 3 519 149 150
Fragments arranged by size:
1108 519 150 149 51 3
BseYI C'CCAG_C
Cuts at: 0 40 391 1257 1897 1980
Size: 40 351 866 640 83
Fragments arranged by size:
866 640 351 83 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1015 1609 1980
Size: 1015 594 371
Fragments arranged by size:
1015 594 371
BsiEI CG_ry'CG
Cuts at: 0 74 1980
Size: 74 1906
BsiHKAI G_wGCw'C
Cuts at: 0 1514 1818 1980
Size: 1514 304 162
Fragments arranged by size:
1514 304 162
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1772 1980
Size: 1772 208
Bsp1286I G_dGCh'C
Cuts at: 0 41 181 1054 1264 1514 1765 1792 1818
Size: 41 140 873 210 250 251 27 26
Cuts at: 1818 1980
Size: 162
Fragments arranged by size:
873 251 250 210 162 140 41 27
26
BspEI T'CCGG_A
Cuts at: 0 419 1391 1980
Size: 419 972 589
Fragments arranged by size:
972 589 419
BspHI T'CATG_A
Cuts at: 0 738 1980
Size: 738 1242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1631 1980
Size: 1631 349
BsrDI GCAATG_nn'
Cuts at: 0 645 822 1289 1980
Size: 645 177 467 691
Fragments arranged by size:
691 645 467 177
BsrFI r'CCGG_y
Cuts at: 0 9 1910 1980
Size: 9 1901 70
Fragments arranged by size:
1901 70 9
BsrGI T'GTAC_A
Cuts at: 0 1590 1980
Size: 1590 390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1360 1980
Size: 1360 620
BstXI CCAn_nnnn'nTGG
Cuts at: 0 165 603 1980
Size: 165 438 1377
Fragments arranged by size:
1377 438 165
BstYI r'GATC_y
Cuts at: 0 689 980 1064 1092 1980
Size: 689 291 84 28 888
Fragments arranged by size:
888 689 291 84 28
BtgI C'CryG_G
Cuts at: 0 65 87 663 1505 1980
Size: 65 22 576 842 475
Fragments arranged by size:
842 576 475 65 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1294 1980
Size: 1294 686
BtsI GCAGTG_nn'
Cuts at: 0 470 606 1908 1980
Size: 470 136 1302 72
Fragments arranged by size:
1302 470 136 72
DraIII CAC_nnn'GTG
Cuts at: 0 1938 1980
Size: 1938 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 1980
Size: 10 1970
EaeI y'GGCC_r
Cuts at: 0 56 71 534 1344 1980
Size: 56 15 463 810 636
Fragments arranged by size:
810 636 463 56 15
EagI C'GGCC_G
Cuts at: 0 71 1980
Size: 71 1909
EarI CTCTTCn'nnn_
Cuts at: 0 328 565 1980
Size: 328 237 1415
Fragments arranged by size:
1415 328 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 358 553 1980
Size: 358 195 1427
Fragments arranged by size:
1427 358 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 697 958 979 1017 1120 1387 1678
Size: 469 228 261 21 38 103 267 291
Cuts at: 1678 1847 1864 1980
Size: 169 17 116
Fragments arranged by size:
469 291 267 261 228 169 116 103
38 21 17
EcoO109I rG'GnC_Cy
Cuts at: 0 507 1761 1762 1788 1952 1980
Size: 507 1254 1 26 164 28
Fragments arranged by size:
1254 507 164 28 26 1
FspI TGC'GCA
Cuts at: 0 1570 1980
Size: 1570 410
HaeII r_GCGC'y
Cuts at: 0 130 954 1383 1932 1980
Size: 130 824 429 549 48
Fragments arranged by size:
824 549 429 130 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 797 1165 1197 1217 1249 1822 1854
Size: 765 32 368 32 20 32 573 32
Cuts at: 1854 1980
Size: 126
Fragments arranged by size:
765 573 368 126 32 32 32 32
20
HincII GTy'rAC
Cuts at: 0 1751 1980
Size: 1751 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1052 1094 1980
Size: 1052 42 886
Fragments arranged by size:
1052 886 42
MscI TGG'CCA
Cuts at: 0 1346 1980
Size: 1346 634
MslI CAynn'nnrTG
Cuts at: 0 572 802 1405 1980
Size: 572 230 603 575
Fragments arranged by size:
603 575 572 230
MspA1I CmG'CkG
Cuts at: 0 67 1638 1864 1973 1980
Size: 67 1571 226 109 7
Fragments arranged by size:
1571 226 109 67 7
NaeI GCC'GGC
Cuts at: 0 1912 1980
Size: 1912 68
NcoI C'CATG_G
Cuts at: 0 1505 1980
Size: 1505 475
NgoMIV G'CCGG_C
Cuts at: 0 1910 1980
Size: 1910 70
NotI GC'GGCC_GC
Cuts at: 0 71 1980
Size: 71 1909
NspI r_CATG'y
Cuts at: 0 801 909 1323 1980
Size: 801 108 414 657
Fragments arranged by size:
801 657 414 108
PasI CC'CwG_GG
Cuts at: 0 1947 1980
Size: 1947 33
PciI A'CATG_T
Cuts at: 0 905 1980
Size: 905 1075
PflMI CCAn_nnn'nTGG
Cuts at: 0 1506 1641 1980
Size: 1506 135 339
Fragments arranged by size:
1506 339 135
PfoI T'CCnGG_A
Cuts at: 0 446 1649 1980
Size: 446 1203 331
Fragments arranged by size:
1203 446 331
PpuMI rG'GwC_Cy
Cuts at: 0 507 1980
Size: 507 1473
PshAI GACnn'nnGTC
Cuts at: 0 908 1980
Size: 908 1072
PspOMI G'GGCC_C
Cuts at: 0 1260 1761 1788 1980
Size: 1260 501 27 192
Fragments arranged by size:
1260 501 192 27
PstI C_TGCA'G
Cuts at: 0 112 1980
Size: 112 1868
PvuII CAG'CTG
Cuts at: 0 1864 1980
Size: 1864 116
SacII CC_GC'GG
Cuts at: 0 68 1980
Size: 68 1912
SalI G'TCGA_C
Cuts at: 0 1749 1980
Size: 1749 231
SapI GCTCTTCn'nnn_
Cuts at: 0 328 1980
Size: 328 1652
SexAI A'CCwGG_T
Cuts at: 0 413 1980
Size: 413 1567
SfcI C'TryA_G
Cuts at: 0 108 1137 1844 1980
Size: 108 1029 707 136
Fragments arranged by size:
1029 707 136 108
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 70 1980
Size: 70 1910
SmaI CCC'GGG
Cuts at: 0 50 1190 1980
Size: 50 1140 790
Fragments arranged by size:
1140 790 50
SphI G_CATG'C
Cuts at: 0 801 1323 1980
Size: 801 522 657
Fragments arranged by size:
801 657 522
StuI AGG'CCT
Cuts at: 0 316 1229 1980
Size: 316 913 751
Fragments arranged by size:
913 751 316
StyI C'CwwG_G
Cuts at: 0 1271 1505 1664 1980
Size: 1271 234 159 316
Fragments arranged by size:
1271 316 234 159
TatI w'GTAC_w
Cuts at: 0 1590 1980
Size: 1590 390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1548 1980
Size: 846 702 432
Fragments arranged by size:
846 702 432
XmaI C'CCGG_G
Cuts at: 0 48 1188 1980
Size: 48 1140 792
Fragments arranged by size:
1140 792 48
XmnI GAAnn'nnTTC
Cuts at: 0 559 1105 1980
Size: 559 546 875
Fragments arranged by size:
875 559 546
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbsI BbvCI BclI BglI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI
NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI
PstI PvuII SacII SalI SapI SexAI SfcI SfiI
SmaI SphI StuI StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AleI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BlpI BmgBI BmtI BpuEI BsaAI
BsaBI BsaHI BsiWI BsmI BsrBI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NruI NsiI
PacI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SanDI SbfI ScaI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XhoI ZraI