Restriction map for
pF1KB5089
(Linear) (Six-base) MAPSORT of: KIBB5089.seq Check: 7403 from: 1 to: 3702
KIBB5089.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 1414 2999 3702
Size: 477 937 1585 703
Fragments arranged by size:
1585 937 703 477
Acc65I G'GTAC_C
Cuts at: 0 2090 3702
Size: 2090 1612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 547 3702
Size: 499 48 3155
Fragments arranged by size:
3155 499 48
AflIII A'CryG_T
Cuts at: 0 1091 2249 3702
Size: 1091 1158 1453
Fragments arranged by size:
1453 1158 1091
AhdI GACnn_n'nnGTC
Cuts at: 0 107 870 2685 3702
Size: 107 763 1815 1017
Fragments arranged by size:
1815 1017 763 107
AleI CACnn'nnGTG
Cuts at: 0 52 1156 1957 3702
Size: 52 1104 801 1745
Fragments arranged by size:
1745 1104 801 52
AlwNI CAG_nnn'CTG
Cuts at: 0 855 966 1374 2211 2697 3123 3192 3471
Size: 855 111 408 837 486 426 69 279
Cuts at: 3471 3702
Size: 231
Fragments arranged by size:
855 837 486 426 408 279 231 111
69
ApaI G_GGCC'C
Cuts at: 0 169 2619 3702
Size: 169 2450 1083
Fragments arranged by size:
2450 1083 169
ApoI r'AATT_y
Cuts at: 0 586 2410 3702
Size: 586 1824 1292
Fragments arranged by size:
1824 1292 586
AvaI C'yCGr_G
Cuts at: 0 768 3640 3702
Size: 768 2872 62
Fragments arranged by size:
2872 768 62
AvrII C'CTAG_G
Cuts at: 0 3033 3702
Size: 3033 669
BamHI G'GATC_C
Cuts at: 0 506 2426 3702
Size: 506 1920 1276
Fragments arranged by size:
1920 1276 506
BanI G'GyrC_C
Cuts at: 0 7 700 1158 1409 1951 2003 2090 2420
Size: 7 693 458 251 542 52 87 330
Cuts at: 2420 3011 3028 3653 3702
Size: 591 17 625 49
Fragments arranged by size:
693 625 591 542 458 330 251 87
52 49 17 7
BanII G_rGCy'C
Cuts at: 0 169 842 1492 1508 1897 2339 2619 2870
Size: 169 673 650 16 389 442 280 251
Cuts at: 2870 3497 3702
Size: 627 205
Fragments arranged by size:
673 650 627 442 389 280 251 205
169 16
BbeI G_GCGC'C
Cuts at: 0 11 704 3702
Size: 11 693 2998
Fragments arranged by size:
2998 693 11
BbvCI CC'TCA_GC
Cuts at: 0 2794 3002 3702
Size: 2794 208 700
Fragments arranged by size:
2794 700 208
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1474 1508 1735 1769 3702
Size: 1474 34 227 34 1933
Fragments arranged by size:
1933 1474 227 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1396 2449 3702
Size: 1396 1053 1253
Fragments arranged by size:
1396 1253 1053
BclI T'GATC_A
Cuts at: 0 2576 3702
Size: 2576 1126
BglI GCCn_nnn'nGGC
Cuts at: 0 801 3514 3575 3591 3702
Size: 801 2713 61 16 111
Fragments arranged by size:
2713 801 111 61 16
BlpI GC'TnA_GC
Cuts at: 0 1930 3702
Size: 1930 1772
Bme1580I G_kGCm'C
Cuts at: 0 169 1735 1954 2423 2619 3033 3656 3702
Size: 169 1566 219 469 196 414 623 46
Fragments arranged by size:
1566 623 469 414 219 196 169 46
BmgBI CAC'GTC
Cuts at: 0 1092 1173 2595 2672 3702
Size: 1092 81 1422 77 1030
Fragments arranged by size:
1422 1092 1030 81 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 2679 3702
Size: 2679 1023
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 120 1527 2669 3206 3702
Size: 120 1407 1142 537 496
Fragments arranged by size:
1407 1142 537 496 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 316 712 748 877 1198 1480 1615
Size: 90 226 396 36 129 321 282 135
Cuts at: 1615 3148 3436 3702
Size: 1533 288 266
Fragments arranged by size:
1533 396 321 288 282 266 226 135
129 90 36
Bpu10I CC'TnA_GC
Cuts at: 0 1480 1555 2654 2794 2990 3002 3202 3702
Size: 1480 75 1099 140 196 12 200 500
Fragments arranged by size:
1480 1099 500 200 196 140 75 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1600 2083 3702
Size: 1600 483 1619
Fragments arranged by size:
1619 1600 483
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 94 857 1532 1858 2708 2909 3702
Size: 52 42 763 675 326 850 201 793
Fragments arranged by size:
850 793 763 675 326 201 52 42
BsaAI yAC'GTr
Cuts at: 0 2238 3702
Size: 2238 1464
BsaHI Gr'CG_yC
Cuts at: 0 8 20 701 3702
Size: 8 12 681 3001
Fragments arranged by size:
3001 681 12 8
BsaWI w'CCGG_w
Cuts at: 0 593 2423 2452 2897 3702
Size: 593 1830 29 445 805
Fragments arranged by size:
1830 805 593 445 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1273 1303 3702
Size: 1273 30 2399
Fragments arranged by size:
2399 1273 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 313 412 922 978 1129 1500 1582
Size: 112 201 99 510 56 151 371 82
Cuts at: 1582 1687 1705 1777 1956 2413 3433 3505 3700
Size: 105 18 72 179 457 1020 72 195
Cuts at: 3700 3702
Size: 2
Fragments arranged by size:
1020 510 457 371 201 195 179 151
112 105 99 82 72 72 56 18
2
BseYI C'CCAG_C
Cuts at: 0 11 959 1024 1360 1417 1521 1531 1588
Size: 11 948 65 336 57 104 10 57
Cuts at: 1588 1982 2697 2728 3161 3454 3509 3573 3594
Size: 394 715 31 433 293 55 64 21
Cuts at: 3594 3702
Size: 108
Fragments arranged by size:
948 715 433 394 336 293 108 104
65 64 57 57 55 31 21 11
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 3136 3702
Size: 388 2748 566
Fragments arranged by size:
2748 566 388
BsiEI CG_ry'CG
Cuts at: 0 670 1108 2122 3702
Size: 670 438 1014 1580
Fragments arranged by size:
1580 1014 670 438
BsiHKAI G_wGCw'C
Cuts at: 0 949 1492 2870 3702
Size: 949 543 1378 832
Fragments arranged by size:
1378 949 832 543
BsmI GAATG_Cn'
Cuts at: 0 1758 3702
Size: 1758 1944
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1295 2662 3702
Size: 1295 1367 1040
Fragments arranged by size:
1367 1295 1040
Bsp1286I G_dGCh'C
Cuts at: 0 169 842 949 1492 1508 1735 1897 1954
Size: 169 673 107 543 16 227 162 57
Cuts at: 1954 2339 2423 2619 2870 3033 3497 3656 3702
Size: 385 84 196 251 163 464 159 46
Fragments arranged by size:
673 543 464 385 251 227 196 169
163 162 159 107 84 57 46 16
BspEI T'CCGG_A
Cuts at: 0 2452 2897 3702
Size: 2452 445 805
Fragments arranged by size:
2452 805 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 1414 1662 2102 2927 2999 3456 3702
Size: 477 937 248 440 825 72 457 246
Fragments arranged by size:
937 825 477 457 440 248 246 72
BsrBI CCG'CTC
Cuts at: 0 642 1289 2450 3702
Size: 642 647 1161 1252
Fragments arranged by size:
1252 1161 647 642
BsrDI GCAATG_nn'
Cuts at: 0 1184 1566 2384 3702
Size: 1184 382 818 1318
Fragments arranged by size:
1318 1184 818 382
BsrFI r'CCGG_y
Cuts at: 0 224 2505 2753 2903 3014 3702
Size: 224 2281 248 150 111 688
Fragments arranged by size:
2281 688 248 224 150 111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3123 3471 3702
Size: 3123 348 231
Fragments arranged by size:
3123 348 231
BstEII G'GTnAC_C
Cuts at: 0 120 3702
Size: 120 3582
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 1875 2736 2859 3504 3702
Size: 54 1821 861 123 645 198
Fragments arranged by size:
1821 861 645 198 123 54
BstYI r'GATC_y
Cuts at: 0 254 506 683 939 2426 3287 3557 3702
Size: 254 252 177 256 1487 861 270 145
Fragments arranged by size:
1487 861 270 256 254 252 177 145
Bsu36I CC'TnA_GG
Cuts at: 0 1112 3702
Size: 1112 2590
BtgI C'CryG_G
Cuts at: 0 50 875 1134 1874 1898 3702
Size: 50 825 259 740 24 1804
Fragments arranged by size:
1804 825 740 259 50 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2059 2808 3702
Size: 2059 749 894
Fragments arranged by size:
2059 894 749
BtsI GCAGTG_nn'
Cuts at: 0 1007 3702
Size: 1007 2695
DraIII CAC_nnn'GTG
Cuts at: 0 970 3702
Size: 970 2732
DrdI GACnn_nn'nnGTC
Cuts at: 0 318 3702
Size: 318 3384
EaeI y'GGCC_r
Cuts at: 0 362 667 1105 1496 2119 2171 2627 2661
Size: 362 305 438 391 623 52 456 34
Cuts at: 2661 3022 3702
Size: 361 680
Fragments arranged by size:
680 623 456 438 391 362 361 305
52 34
EagI C'GGCC_G
Cuts at: 0 667 1105 2119 3702
Size: 667 438 1014 1583
Fragments arranged by size:
1583 1014 667 438
EarI CTCTTCn'nnn_
Cuts at: 0 1682 1993 2327 3482 3702
Size: 1682 311 334 1155 220
Fragments arranged by size:
1682 1155 334 311 220
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3370 3550 3702
Size: 3370 180 152
Fragments arranged by size:
3370 180 152
EcoICRI GAG'CTC
Cuts at: 0 1490 2868 3702
Size: 1490 1378 834
Fragments arranged by size:
1490 1378 834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 316 499 547 712 748 877 1198
Size: 90 226 183 48 165 36 129 321
Cuts at: 1198 1480 1615 3148 3436 3702
Size: 282 135 1533 288 266
Fragments arranged by size:
1533 321 288 282 266 226 183 165
135 129 90 48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 2839 2989 3184 3702
Size: 241 2598 150 195 518
Fragments arranged by size:
2598 518 241 195 150
EcoO109I rG'GnC_Cy
Cuts at: 0 166 246 280 1028 1371 1623 1636 2571
Size: 166 80 34 748 343 252 13 935
Cuts at: 2571 2615 3468 3702
Size: 44 853 234
Fragments arranged by size:
935 853 748 343 252 234 166 80
44 34 13
FspI TGC'GCA
Cuts at: 0 460 2932 3702
Size: 460 2472 770
Fragments arranged by size:
2472 770 460
HaeII r_GCGC'y
Cuts at: 0 11 704 1442 3306 3668 3702
Size: 11 693 738 1864 362 34
Fragments arranged by size:
1864 738 693 362 34 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 856 888 1753 1785 1837 1869
Size: 93 32 731 32 865 32 52 32
Cuts at: 1869 2671 2703 3360 3392 3702
Size: 802 32 657 32 310
Fragments arranged by size:
865 802 731 657 310 93 52 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1782 3399 3702
Size: 1782 1617 303
Fragments arranged by size:
1782 1617 303
KasI G'GCGC_C
Cuts at: 0 7 700 3702
Size: 7 693 3002
Fragments arranged by size:
3002 693 7
KpnI G_GTAC'C
Cuts at: 0 2094 3702
Size: 2094 1608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 91 484 1994 2240 3702
Size: 17 74 393 1510 246 1462
Fragments arranged by size:
1510 1462 393 246 74 17
MscI TGG'CCA
Cuts at: 0 2173 2663 3702
Size: 2173 490 1039
Fragments arranged by size:
2173 1039 490
MslI CAynn'nnrTG
Cuts at: 0 52 178 364 952 1062 1156 1957 2236
Size: 52 126 186 588 110 94 801 279
Cuts at: 2236 3702
Size: 1466
Fragments arranged by size:
1466 801 588 279 186 126 110 94
52
MspA1I CmG'CkG
Cuts at: 0 1020 1076 1338 1521 1544 2087 2496 2625
Size: 1020 56 262 183 23 543 409 129
Cuts at: 2625 2697 2859 2946 3046 3165 3198 3435 3480
Size: 72 162 87 100 119 33 237 45
Cuts at: 3480 3513 3537 3650 3702
Size: 33 24 113 52
Fragments arranged by size:
1020 543 409 262 237 183 162 129
119 113 100 87 72 56 52 45
33 33 24 23
NaeI GCC'GGC
Cuts at: 0 2507 2755 2905 3016 3702
Size: 2507 248 150 111 686
Fragments arranged by size:
2507 686 248 150 111
NarI GG'CG_CC
Cuts at: 0 8 701 3702
Size: 8 693 3001
Fragments arranged by size:
3001 693 8
NcoI C'CATG_G
Cuts at: 0 875 1874 1898 3702
Size: 875 999 24 1804
Fragments arranged by size:
1804 999 875 24
NgoMIV G'CCGG_C
Cuts at: 0 2505 2753 2903 3014 3702
Size: 2505 248 150 111 688
Fragments arranged by size:
2505 688 248 150 111
NspI r_CATG'y
Cuts at: 0 1980 2253 3702
Size: 1980 273 1449
Fragments arranged by size:
1980 1449 273
PasI CC'CwG_GG
Cuts at: 0 2803 3702
Size: 2803 899
PciI A'CATG_T
Cuts at: 0 2249 3702
Size: 2249 1453
PflMI CCAn_nnn'nTGG
Cuts at: 0 389 3702
Size: 389 3313
PfoI T'CCnGG_A
Cuts at: 0 257 442 3290 3702
Size: 257 185 2848 412
Fragments arranged by size:
2848 412 257 185
PpuMI rG'GwC_Cy
Cuts at: 0 246 280 1028 1371 3468 3702
Size: 246 34 748 343 2097 234
Fragments arranged by size:
2097 748 343 246 234 34
PspOMI G'GGCC_C
Cuts at: 0 165 2615 3702
Size: 165 2450 1087
Fragments arranged by size:
2450 1087 165
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3082 3114 3702
Size: 3082 32 588
Fragments arranged by size:
3082 588 32
PstI C_TGCA'G
Cuts at: 0 35 488 3197 3284 3702
Size: 35 453 2709 87 418
Fragments arranged by size:
2709 453 418 87 35
PvuII CAG'CTG
Cuts at: 0 1020 1521 1544 2625 2697 3480 3513 3702
Size: 1020 501 23 1081 72 783 33 189
Fragments arranged by size:
1081 1020 783 501 189 72 33 23
SacI G_AGCT'C
Cuts at: 0 1492 2870 3702
Size: 1492 1378 832
Fragments arranged by size:
1492 1378 832
SanDI GG'GwC_CC
Cuts at: 0 280 3702
Size: 280 3422
SapI GCTCTTCn'nnn_
Cuts at: 0 1993 2327 3702
Size: 1993 334 1375
Fragments arranged by size:
1993 1375 334
ScaI AGT'ACT
Cuts at: 0 1282 3702
Size: 1282 2420
SexAI A'CCwGG_T
Cuts at: 0 116 3702
Size: 116 3586
SfcI C'TryA_G
Cuts at: 0 31 484 1721 3193 3280 3702
Size: 31 453 1237 1472 87 422
Fragments arranged by size:
1472 1237 453 422 87 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3514 3702
Size: 3514 188
SfoI GGC'GCC
Cuts at: 0 9 702 3702
Size: 9 693 3000
Fragments arranged by size:
3000 693 9
SmaI CCC'GGG
Cuts at: 0 3642 3702
Size: 3642 60
SmlI C'TyrA_G
Cuts at: 0 1579 2098 3702
Size: 1579 519 1604
Fragments arranged by size:
1604 1579 519
StuI AGG'CCT
Cuts at: 0 1553 3702
Size: 1553 2149
StyI C'CwwG_G
Cuts at: 0 242 383 626 704 875 1868 1874 1898
Size: 242 141 243 78 171 993 6 24
Cuts at: 1898 1946 2351 3033 3702
Size: 48 405 682 669
Fragments arranged by size:
993 682 669 405 243 242 171 141
78 48 24 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1603 2698 3702
Size: 1603 1095 1004
Fragments arranged by size:
1603 1095 1004
TatI w'GTAC_w
Cuts at: 0 155 1280 2072 3702
Size: 155 1125 792 1630
Fragments arranged by size:
1630 1125 792 155
Tth111I GACn'n_nGTC
Cuts at: 0 1032 3702
Size: 1032 2670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 177 1896 1905 3411 3702
Size: 114 63 1719 9 1506 291
Fragments arranged by size:
1719 1506 291 114 63 9
XmaI C'CCGG_G
Cuts at: 0 3640 3702
Size: 3640 62
XmnI GAAnn'nnTTC
Cuts at: 0 451 532 2311 3702
Size: 451 81 1779 1391
Fragments arranged by size:
1779 1391 451 81
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AleI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII
SacI SanDI SapI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BbsI BfrBI BglII
BmtI BsaBI BsiWI BspHI BsrGI BssHII BssSI BstBI
BstZ17I ClaI CspCI DraI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI RsrII SacII SalI SbfI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XhoI ZraI