Restriction map for
pF1KB5090
(Linear) (Six-base) MAPSORT of: KIBB5090.seq Check: 2653 from: 1 to: 2307
KIBB5090.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
Acc65I G'GTAC_C
Cuts at: 0 38 2307
Size: 38 2269
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 427 808 2105 2134 2307
Size: 253 174 381 1297 29 173
Fragments arranged by size:
1297 381 253 174 173 29
AflIII A'CryG_T
Cuts at: 0 1940 2307
Size: 1940 367
AleI CACnn'nnGTG
Cuts at: 0 163 1174 1867 2307
Size: 163 1011 693 440
Fragments arranged by size:
1011 693 440 163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 761 1006 1834 2307
Size: 761 245 828 473
Fragments arranged by size:
828 761 473 245
AlwNI CAG_nnn'CTG
Cuts at: 0 99 361 429 606 1932 2101 2307
Size: 99 262 68 177 1326 169 206
Fragments arranged by size:
1326 262 206 177 169 99 68
ApoI r'AATT_y
Cuts at: 0 1060 1147 1219 1642 2307
Size: 1060 87 72 423 665
Fragments arranged by size:
1060 665 423 87 72
BamHI G'GATC_C
Cuts at: 0 1138 2307
Size: 1138 1169
BanI G'GyrC_C
Cuts at: 0 38 320 1810 2307
Size: 38 282 1490 497
Fragments arranged by size:
1490 497 282 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 2122 2307
Size: 2122 185
BbvCI CC'TCA_GC
Cuts at: 0 2235 2307
Size: 2235 72
BclI T'GATC_A
Cuts at: 0 1313 2307
Size: 1313 994
BfrBI ATG'CAT
Cuts at: 0 993 2307
Size: 993 1314
BglI GCCn_nnn'nGGC
Cuts at: 0 364 2307
Size: 364 1943
BglII A'GATC_T
Cuts at: 0 558 2307
Size: 558 1749
Bme1580I G_kGCm'C
Cuts at: 0 1165 2307
Size: 1165 1142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1386 2298 2307
Size: 1386 912 9
Fragments arranged by size:
1386 912 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 451 487 871 934 1790 2307
Size: 64 387 36 384 63 856 517
Fragments arranged by size:
856 517 387 384 64 63 36
Bpu10I CC'TnA_GC
Cuts at: 0 1522 2235 2307
Size: 1522 713 72
Fragments arranged by size:
1522 713 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 520 1333 1812 2307
Size: 304 216 813 479 495
Fragments arranged by size:
813 495 479 304 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 1318 1357 2307
Size: 1318 39 950
Fragments arranged by size:
1318 950 39
BsaAI yAC'GTr
Cuts at: 0 690 2307
Size: 690 1617
BsaHI Gr'CG_yC
Cuts at: 0 1025 1423 1853 2307
Size: 1025 398 430 454
Fragments arranged by size:
1025 454 430 398
BsaWI w'CCGG_w
Cuts at: 0 452 2307
Size: 452 1855
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1788 1818 2307
Size: 1788 30 489
Fragments arranged by size:
1788 489 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 868 931 1315 1527 2053 2307
Size: 727 141 63 384 212 526 254
Fragments arranged by size:
727 526 384 254 212 141 63
BseYI C'CCAG_C
Cuts at: 0 368 2307
Size: 368 1939
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 712 1291 2307
Size: 484 228 579 1016
Fragments arranged by size:
1016 579 484 228
BsiEI CG_ry'CG
Cuts at: 0 907 2307
Size: 907 1400
BsiHKAI G_wGCw'C
Cuts at: 0 1798 2307
Size: 1798 509
BsmI GAATG_Cn'
Cuts at: 0 2044 2307
Size: 2044 263
Bsp1286I G_dGCh'C
Cuts at: 0 1165 1798 2307
Size: 1165 633 509
Fragments arranged by size:
1165 633 509
BspEI T'CCGG_A
Cuts at: 0 452 2307
Size: 452 1855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 2155 2174 2307
Size: 266 1889 19 133
Fragments arranged by size:
1889 266 133 19
BsrBI CCG'CTC
Cuts at: 0 972 1776 1782 2307
Size: 972 804 6 525
Fragments arranged by size:
972 804 525 6
BsrFI r'CCGG_y
Cuts at: 0 902 1001 1021 2307
Size: 902 99 20 1286
Fragments arranged by size:
1286 902 99 20
BsrGI T'GTAC_A
Cuts at: 0 894 2307
Size: 894 1413
BstAPI GCAn_nnn'nTGC
Cuts at: 0 361 426 597 2307
Size: 361 65 171 1710
Fragments arranged by size:
1710 361 171 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1418 1995 2307
Size: 1418 577 312
Fragments arranged by size:
1418 577 312
BstYI r'GATC_y
Cuts at: 0 468 558 1138 1898 2307
Size: 468 90 580 760 409
Fragments arranged by size:
760 580 468 409 90
Bsu36I CC'TnA_GG
Cuts at: 0 1237 2307
Size: 1237 1070
BtgI C'CryG_G
Cuts at: 0 404 1907 2307
Size: 404 1503 400
Fragments arranged by size:
1503 404 400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 694 2307
Size: 694 1613
BtsI GCAGTG_nn'
Cuts at: 0 359 620 2307
Size: 359 261 1687
Fragments arranged by size:
1687 359 261
EaeI y'GGCC_r
Cuts at: 0 137 904 1511 2062 2307
Size: 137 767 607 551 245
Fragments arranged by size:
767 607 551 245 137
EagI C'GGCC_G
Cuts at: 0 904 2307
Size: 904 1403
EarI CTCTTCn'nnn_
Cuts at: 0 451 724 2307
Size: 451 273 1583
Fragments arranged by size:
1583 451 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 253 427 451 487 808 871 934
Size: 64 189 174 24 36 321 63 63
Cuts at: 934 1790 2105 2134 2307
Size: 856 315 29 173
Fragments arranged by size:
856 321 315 189 174 173 64 63
63 36 29 24
EcoO109I rG'GnC_Cy
Cuts at: 0 885 1518 1600 2307
Size: 885 633 82 707
Fragments arranged by size:
885 707 633 82
EcoRI G'AATT_C
Cuts at: 0 1219 2307
Size: 1219 1088
HaeII r_GCGC'y
Cuts at: 0 2102 2307
Size: 2102 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 264 296 1096 1128 1440 1472
Size: 102 32 130 32 800 32 312 32
Cuts at: 1472 1547 1579 2307
Size: 75 32 728
Fragments arranged by size:
800 728 312 130 102 75 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 835 2279 2307
Size: 835 1444 28
Fragments arranged by size:
1444 835 28
KpnI G_GTAC'C
Cuts at: 0 42 2307
Size: 42 2265
MfeI C'AATT_G
Cuts at: 0 1272 1490 2307
Size: 1272 218 817
Fragments arranged by size:
1272 817 218
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1418 2307
Size: 1220 198 889
Fragments arranged by size:
1220 889 198
MslI CAynn'nnrTG
Cuts at: 0 163 988 1174 1867 2307
Size: 163 825 186 693 440
Fragments arranged by size:
825 693 440 186 163
MspA1I CmG'CkG
Cuts at: 0 606 1572 1886 1932 2307
Size: 606 966 314 46 375
Fragments arranged by size:
966 606 375 314 46
NdeI CA'TA_TG
Cuts at: 0 132 2307
Size: 132 2175
NsiI A_TGCA'T
Cuts at: 0 995 2307
Size: 995 1312
NspI r_CATG'y
Cuts at: 0 157 555 987 1446 1944 2307
Size: 157 398 432 459 498 363
Fragments arranged by size:
498 459 432 398 363 157
PciI A'CATG_T
Cuts at: 0 1940 2307
Size: 1940 367
PflMI CCAn_nnn'nTGG
Cuts at: 0 1433 1908 2307
Size: 1433 475 399
Fragments arranged by size:
1433 475 399
PfoI T'CCnGG_A
Cuts at: 0 503 1141 2307
Size: 503 638 1166
Fragments arranged by size:
1166 638 503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 602 634 2307
Size: 602 32 1673
Fragments arranged by size:
1673 602 32
PpuMI rG'GwC_Cy
Cuts at: 0 885 1518 1600 2307
Size: 885 633 82 707
Fragments arranged by size:
885 707 633 82
PshAI GACnn'nnGTC
Cuts at: 0 1024 2307
Size: 1024 1283
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1732 1764 2307
Size: 1732 32 543
Fragments arranged by size:
1732 543 32
PstI C_TGCA'G
Cuts at: 0 357 1218 2152 2307
Size: 357 861 934 155
Fragments arranged by size:
934 861 357 155
PvuII CAG'CTG
Cuts at: 0 606 1572 1932 2307
Size: 606 966 360 375
Fragments arranged by size:
966 606 375 360
SanDI GG'GwC_CC
Cuts at: 0 885 2307
Size: 885 1422
SexAI A'CCwGG_T
Cuts at: 0 1602 2307
Size: 1602 705
SfcI C'TryA_G
Cuts at: 0 19 63 346 353 1214 2148 2307
Size: 19 44 283 7 861 934 159
Fragments arranged by size:
934 861 283 159 44 19 7
SmlI C'TyrA_G
Cuts at: 0 283 535 1348 1791 2307
Size: 283 252 813 443 516
Fragments arranged by size:
813 516 443 283 252
SphI G_CATG'C
Cuts at: 0 987 2307
Size: 987 1320
StuI AGG'CCT
Cuts at: 0 1346 2307
Size: 1346 961
StyI C'CwwG_G
Cuts at: 0 106 1341 2033 2307
Size: 106 1235 692 274
Fragments arranged by size:
1235 692 274 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1342 1857 2307
Size: 1342 515 450
Fragments arranged by size:
1342 515 450
TatI w'GTAC_w
Cuts at: 0 894 1721 2307
Size: 894 827 586
Fragments arranged by size:
894 827 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1592 1624 1788 1820 2307
Size: 1592 32 164 32 487
Fragments arranged by size:
1592 487 164 32 32
XbaI T'CTAG_A
Cuts at: 0 471 2307
Size: 471 1836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 402 1496 2307
Size: 402 1094 811
Fragments arranged by size:
1094 811 402
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AlwNI ApoI BamHI
BanI BbsI BbvCI BclI BfrBI BglI BglII Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII KpnI MfeI MmeI
MslI MspA1I NdeI NsiI NspI PciI PflMI PfoI
PpiI PpuMI PshAI PsrI PstI PvuII SanDI SexAI
SfcI SmlI SphI StuI StyI TaqII TatI TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanII BbeI BcgI BciVI BlpI BmgBI BmrI
BmtI BsaBI BsiWI BsmBI BspHI BsrDI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XhoI XmaI
XmnI ZraI