Restriction map for
pF1KB5091
(Linear) (Six-base) MAPSORT of: KIBB5091.seq Check: 1699 from: 1 to: 1812
KIBB5091.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AclI AA'CG_TT
Cuts at: 0 257 1797 1812
Size: 257 1540 15
Fragments arranged by size:
1540 257 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1812
Size: 1393 419
AleI CACnn'nnGTG
Cuts at: 0 1013 1812
Size: 1013 799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 75 1812
Size: 75 1737
ApaLI G'TGCA_C
Cuts at: 0 220 1812
Size: 220 1592
ApoI r'AATT_y
Cuts at: 0 172 1036 1096 1145 1812
Size: 172 864 60 49 667
Fragments arranged by size:
864 667 172 60 49
BanI G'GyrC_C
Cuts at: 0 840 1812
Size: 840 972
BanII G_rGCy'C
Cuts at: 0 411 538 1718 1812
Size: 411 127 1180 94
Fragments arranged by size:
1180 411 127 94
BciVI GTATCCnnnnn_n'
Cuts at: 0 752 1478 1812
Size: 752 726 334
Fragments arranged by size:
752 726 334
BlpI GC'TnA_GC
Cuts at: 0 40 1812
Size: 40 1772
Bme1580I G_kGCm'C
Cuts at: 0 224 1812
Size: 224 1588
BmrI ACTGGGnnnn_n'
Cuts at: 0 1212 1812
Size: 1212 600
Bpu10I CC'TnA_GC
Cuts at: 0 797 1812
Size: 797 1015
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1032 1812
Size: 773 259 780
Fragments arranged by size:
780 773 259
BsaBI GATnn'nnATC
Cuts at: 0 1772 1812
Size: 1772 40
BsaWI w'CCGG_w
Cuts at: 0 168 1756 1812
Size: 168 1588 56
Fragments arranged by size:
1588 168 56
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 776 1812
Size: 776 1036
BseYI C'CCAG_C
Cuts at: 0 371 563 627 1812
Size: 371 192 64 1185
Fragments arranged by size:
1185 371 192 64
BsiHKAI G_wGCw'C
Cuts at: 0 224 1539 1718 1812
Size: 224 1315 179 94
Fragments arranged by size:
1315 224 179 94
BsmI GAATG_Cn'
Cuts at: 0 553 1812
Size: 553 1259
Bsp1286I G_dGCh'C
Cuts at: 0 224 411 538 1539 1718 1812
Size: 224 187 127 1001 179 94
Fragments arranged by size:
1001 224 187 179 127 94
BspEI T'CCGG_A
Cuts at: 0 1756 1812
Size: 1756 56
BspHI T'CATG_A
Cuts at: 0 1149 1812
Size: 1149 663
BsrBI CCG'CTC
Cuts at: 0 1754 1812
Size: 1754 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 559 1812
Size: 559 1253
BstBI TT'CG_AA
Cuts at: 0 282 1812
Size: 282 1530
BstXI CCAn_nnnn'nTGG
Cuts at: 0 627 1812
Size: 627 1185
BstYI r'GATC_y
Cuts at: 0 1707 1812
Size: 1707 105
BtgI C'CryG_G
Cuts at: 0 633 1520 1812
Size: 633 887 292
Fragments arranged by size:
887 633 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 911 1812
Size: 911 901
BtsI GCAGTG_nn'
Cuts at: 0 1637 1812
Size: 1637 175
ClaI AT'CG_AT
Cuts at: 0 1451 1812
Size: 1451 361
EaeI y'GGCC_r
Cuts at: 0 1495 1812
Size: 1495 317
EcoICRI GAG'CTC
Cuts at: 0 1716 1812
Size: 1716 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1812
Size: 1393 419
EcoNI CCTnn'n_nnAGG
Cuts at: 0 697 1812
Size: 697 1115
EcoRV GAT'ATC
Cuts at: 0 1770 1812
Size: 1770 42
HaeII r_GCGC'y
Cuts at: 0 40 1812
Size: 40 1772
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 318 350 729 761 768 800 1005 1037
Size: 318 32 379 32 7 32 205 32
Cuts at: 1037 1812
Size: 775
Fragments arranged by size:
775 379 318 205 32 32 32 32
7
HincII GTy'rAC
Cuts at: 0 308 1812
Size: 308 1504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1409 1512 1812
Size: 1409 103 300
Fragments arranged by size:
1409 300 103
MslI CAynn'nnrTG
Cuts at: 0 1013 1812
Size: 1013 799
NcoI C'CATG_G
Cuts at: 0 633 1520 1812
Size: 633 887 292
Fragments arranged by size:
887 633 292
PasI CC'CwG_GG
Cuts at: 0 531 1812
Size: 531 1281
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 869 1812
Size: 639 230 943
Fragments arranged by size:
943 639 230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 925 957 1812
Size: 925 32 855
Fragments arranged by size:
925 855 32
PstI C_TGCA'G
Cuts at: 0 1425 1812
Size: 1425 387
SacI G_AGCT'C
Cuts at: 0 1718 1812
Size: 1718 94
ScaI AGT'ACT
Cuts at: 0 1784 1812
Size: 1784 28
SfcI C'TryA_G
Cuts at: 0 1421 1812
Size: 1421 391
SmlI C'TyrA_G
Cuts at: 0 788 1011 1812
Size: 788 223 801
Fragments arranged by size:
801 788 223
StyI C'CwwG_G
Cuts at: 0 443 633 1520 1812
Size: 443 190 887 292
Fragments arranged by size:
887 443 292 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 436 1812
Size: 86 350 1376
Fragments arranged by size:
1376 350 86
TatI w'GTAC_w
Cuts at: 0 1287 1782 1812
Size: 1287 495 30
Fragments arranged by size:
1287 495 30
Enzymes that do cut:
AclI AcuI AleI AlfI ApaLI ApoI BanI BanII
BciVI BlpI Bme1580I BmrI Bpu10I BpuEI BsaBI BsaWI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
EaeI EcoICRI Eco57MI EcoNI EcoRV HaeII Hin4I HincII
MmeI MslI NcoI PasI PflMI PpiI PstI SacI
ScaI SfcI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BmgBI BmtI BplI BpmI BsaI
BsaAI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I
CspCI DraI DraIII DrdI EagI EarI EciI EcoO109I
EcoRI FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI