Restriction map for
pF1KB5093
(Linear) (Six-base) MAPSORT of: KIBB5093.seq Check: 6717 from: 1 to: 2853
KIBB5093.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AclI AA'CG_TT
Cuts at: 0 2125 2853
Size: 2125 728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2464 2853
Size: 2464 389
AfeI AGC'GCT
Cuts at: 0 182 2853
Size: 182 2671
AflII C'TTAA_G
Cuts at: 0 819 2853
Size: 819 2034
AflIII A'CryG_T
Cuts at: 0 2451 2853
Size: 2451 402
AlwNI CAG_nnn'CTG
Cuts at: 0 543 952 1850 2853
Size: 543 409 898 1003
Fragments arranged by size:
1003 898 543 409
ApaI G_GGCC'C
Cuts at: 0 10 62 2853
Size: 10 52 2791
Fragments arranged by size:
2791 52 10
ApaLI G'TGCA_C
Cuts at: 0 508 2853
Size: 508 2345
ApoI r'AATT_y
Cuts at: 0 715 1106 1208 1440 1819 2853
Size: 715 391 102 232 379 1034
Fragments arranged by size:
1034 715 391 379 232 102
AseI AT'TA_AT
Cuts at: 0 1906 2158 2853
Size: 1906 252 695
Fragments arranged by size:
1906 695 252
AvaI C'yCGr_G
Cuts at: 0 154 433 2853
Size: 154 279 2420
Fragments arranged by size:
2420 279 154
AvrII C'CTAG_G
Cuts at: 0 2010 2853
Size: 2010 843
BanI G'GyrC_C
Cuts at: 0 72 90 501 1147 2853
Size: 72 18 411 646 1706
Fragments arranged by size:
1706 646 411 72 18
BanII G_rGCy'C
Cuts at: 0 10 62 164 2853
Size: 10 52 102 2689
Fragments arranged by size:
2689 102 52 10
BbeI G_GCGC'C
Cuts at: 0 76 94 2853
Size: 76 18 2759
Fragments arranged by size:
2759 76 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 2435 2853
Size: 2435 418
BbvCI CC'TCA_GC
Cuts at: 0 517 2853
Size: 517 2336
BciVI GTATCCnnnnn_n'
Cuts at: 0 583 2853
Size: 583 2270
BclI T'GATC_A
Cuts at: 0 2312 2853
Size: 2312 541
BfrBI ATG'CAT
Cuts at: 0 226 2853
Size: 226 2627
BglI GCCn_nnn'nGGC
Cuts at: 0 159 2002 2853
Size: 159 1843 851
Fragments arranged by size:
1843 851 159
BglII A'GATC_T
Cuts at: 0 563 1182 2853
Size: 563 619 1671
Fragments arranged by size:
1671 619 563
BlpI GC'TnA_GC
Cuts at: 0 163 2853
Size: 163 2690
Bme1580I G_kGCm'C
Cuts at: 0 10 62 155 512 2853
Size: 10 52 93 357 2341
Fragments arranged by size:
2341 357 93 52 10
BmgBI CAC'GTC
Cuts at: 0 2452 2853
Size: 2452 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 356 1615 2853
Size: 356 1259 1238
Fragments arranged by size:
1259 1238 356
BmtI G_CTAG'C
Cuts at: 0 1915 2853
Size: 1915 938
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2226 2853
Size: 2226 627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 700 1450 2853
Size: 319 381 750 1403
Fragments arranged by size:
1403 750 381 319
Bpu10I CC'TnA_GC
Cuts at: 0 517 1033 1350 2853
Size: 517 516 317 1503
Fragments arranged by size:
1503 517 516 317
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 2853
Size: 552 2301
BsaI GGTCTCn'nnnn_
Cuts at: 0 676 1061 2853
Size: 676 385 1792
Fragments arranged by size:
1792 676 385
BsaBI GATnn'nnATC
Cuts at: 0 809 2853
Size: 809 2044
BsaHI Gr'CG_yC
Cuts at: 0 73 91 2853
Size: 73 18 2762
Fragments arranged by size:
2762 73 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1872 2853
Size: 1872 981
BseYI C'CCAG_C
Cuts at: 0 61 1861 2853
Size: 61 1800 992
Fragments arranged by size:
1800 992 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 2788 2853
Size: 946 1842 65
Fragments arranged by size:
1842 946 65
BsiEI CG_ry'CG
Cuts at: 0 56 114 149 2853
Size: 56 58 35 2704
Fragments arranged by size:
2704 58 56 35
BsiHKAI G_wGCw'C
Cuts at: 0 512 2853
Size: 512 2341
Bsp1286I G_dGCh'C
Cuts at: 0 10 62 155 164 512 2853
Size: 10 52 93 9 348 2341
Fragments arranged by size:
2341 348 93 52 10 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1676 2853
Size: 1676 1177
BsrDI GCAATG_nn'
Cuts at: 0 2371 2853
Size: 2371 482
BsrGI T'GTAC_A
Cuts at: 0 1564 1699 2853
Size: 1564 135 1154
Fragments arranged by size:
1564 1154 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 399 1850 2853
Size: 399 1451 1003
Fragments arranged by size:
1451 1003 399
BstYI r'GATC_y
Cuts at: 0 563 1182 2853
Size: 563 619 1671
Fragments arranged by size:
1671 619 563
Bsu36I CC'TnA_GG
Cuts at: 0 722 1375 2675 2853
Size: 722 653 1300 178
Fragments arranged by size:
1300 722 653 178
BtsI GCAGTG_nn'
Cuts at: 0 1705 1851 2315 2660 2853
Size: 1705 146 464 345 193
Fragments arranged by size:
1705 464 345 193 146
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2448 2483 2853
Size: 2448 35 370
Fragments arranged by size:
2448 370 35
DraI TTT'AAA
Cuts at: 0 1445 2424 2853
Size: 1445 979 429
Fragments arranged by size:
1445 979 429
DraIII CAC_nnn'GTG
Cuts at: 0 1479 2853
Size: 1479 1374
EaeI y'GGCC_r
Cuts at: 0 53 146 2162 2853
Size: 53 93 2016 691
Fragments arranged by size:
2016 691 93 53
EagI C'GGCC_G
Cuts at: 0 53 146 2853
Size: 53 93 2707
Fragments arranged by size:
2707 93 53
EarI CTCTTCn'nnn_
Cuts at: 0 245 1957 2853
Size: 245 1712 896
Fragments arranged by size:
1712 896 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 700 1450 2464 2853
Size: 319 381 750 1014 389
Fragments arranged by size:
1014 750 389 381 319
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1289 1574 2853
Size: 1289 285 1279
Fragments arranged by size:
1289 1279 285
EcoO109I rG'GnC_Cy
Cuts at: 0 2205 2853
Size: 2205 648
EcoRV GAT'ATC
Cuts at: 0 189 1166 2853
Size: 189 977 1687
Fragments arranged by size:
1687 977 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 989 2853
Size: 989 1864
HaeII r_GCGC'y
Cuts at: 0 76 94 170 184 544 2853
Size: 76 18 76 14 360 2309
Fragments arranged by size:
2309 360 76 76 18 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 1167 1199 1608 1640 1783 1815
Size: 537 32 598 32 409 32 143 32
Cuts at: 1815 1956 1988 2853
Size: 141 32 865
Fragments arranged by size:
865 598 537 409 143 141 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 652 1371 2853
Size: 652 719 1482
Fragments arranged by size:
1482 719 652
HindIII A'AGCT_T
Cuts at: 0 1929 2853
Size: 1929 924
HpaI GTT'AAC
Cuts at: 0 1371 2853
Size: 1371 1482
KasI G'GCGC_C
Cuts at: 0 72 90 2853
Size: 72 18 2763
Fragments arranged by size:
2763 72 18
MfeI C'AATT_G
Cuts at: 0 259 2265 2853
Size: 259 2006 588
Fragments arranged by size:
2006 588 259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2714 2853
Size: 2714 139
MslI CAynn'nnrTG
Cuts at: 0 836 2853
Size: 836 2017
MspA1I CmG'CkG
Cuts at: 0 65 101 2639 2853
Size: 65 36 2538 214
Fragments arranged by size:
2538 214 65 36
NarI GG'CG_CC
Cuts at: 0 73 91 2853
Size: 73 18 2762
Fragments arranged by size:
2762 73 18
NdeI CA'TA_TG
Cuts at: 0 1395 2853
Size: 1395 1458
NheI G'CTAG_C
Cuts at: 0 1911 2853
Size: 1911 942
NsiI A_TGCA'T
Cuts at: 0 228 2853
Size: 228 2625
NspI r_CATG'y
Cuts at: 0 2245 2541 2853
Size: 2245 296 312
Fragments arranged by size:
2245 312 296
PasI CC'CwG_GG
Cuts at: 0 36 2853
Size: 36 2817
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 1098 1479 2853
Size: 183 915 381 1374
Fragments arranged by size:
1374 915 381 183
PfoI T'CCnGG_A
Cuts at: 0 130 1466 2853
Size: 130 1336 1387
Fragments arranged by size:
1387 1336 130
PpuMI rG'GwC_Cy
Cuts at: 0 2205 2853
Size: 2205 648
PsiI TTA'TAA
Cuts at: 0 701 2853
Size: 701 2152
PspOMI G'GGCC_C
Cuts at: 0 6 58 2853
Size: 6 52 2795
Fragments arranged by size:
2795 52 6
PstI C_TGCA'G
Cuts at: 0 100 2853
Size: 100 2753
PvuII CAG'CTG
Cuts at: 0 101 2639 2853
Size: 101 2538 214
Fragments arranged by size:
2538 214 101
SapI GCTCTTCn'nnn_
Cuts at: 0 245 1957 2853
Size: 245 1712 896
Fragments arranged by size:
1712 896 245
SexAI A'CCwGG_T
Cuts at: 0 2762 2853
Size: 2762 91
SfcI C'TryA_G
Cuts at: 0 96 535 2018 2090 2853
Size: 96 439 1483 72 763
Fragments arranged by size:
1483 763 439 96 72
SfoI GGC'GCC
Cuts at: 0 74 92 2853
Size: 74 18 2761
Fragments arranged by size:
2761 74 18
SmlI C'TyrA_G
Cuts at: 0 567 819 2853
Size: 567 252 2034
Fragments arranged by size:
2034 567 252
SspI AAT'ATT
Cuts at: 0 1600 2059 2853
Size: 1600 459 794
Fragments arranged by size:
1600 794 459
StuI AGG'CCT
Cuts at: 0 298 1160 1295 2773 2853
Size: 298 862 135 1478 80
Fragments arranged by size:
1478 862 298 135 80
StyI C'CwwG_G
Cuts at: 0 2010 2496 2853
Size: 2010 486 357
Fragments arranged by size:
2010 486 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 305 2853
Size: 190 115 2548
Fragments arranged by size:
2548 190 115
TatI w'GTAC_w
Cuts at: 0 1564 1699 2540 2853
Size: 1564 135 841 313
Fragments arranged by size:
1564 841 313 135
XbaI T'CTAG_A
Cuts at: 0 246 1179 2853
Size: 246 933 1674
Fragments arranged by size:
1674 933 246
XmnI GAAnn'nnTTC
Cuts at: 0 371 2588 2853
Size: 371 2217 265
Fragments arranged by size:
2217 371 265
Enzymes that do cut:
AclI AcuI AfeI AflII AflIII AlwNI ApaI ApaLI
ApoI AseI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrGI BstAPI BstYI Bsu36I BtsI CspCI
DraI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MmeI MslI MspA1I NarI NdeI NheI NsiI
NspI PasI PflMI PfoI PpuMI PsiI PspOMI PstI
PvuII SapI SexAI SfcI SfoI SmlI SspI StuI
StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AgeI AhdI AleI AlfI
AloI AscI AsiSI BaeI BamHI BcgI BsaAI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI BtgZI
ClaI DrdI EciI EcoICRI EcoRI FseI FspI FspAI
KpnI MluI MscI NaeI NcoI NgoMIV NotI NruI
PacI PciI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TstI Tth111I XcmI XhoI XmaI ZraI