Restriction map for
pF1KB5096
(Linear) (Six-base) MAPSORT of: KIBB5096.seq Check: 9488 from: 1 to: 1746
KIBB5096.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1492 1668 1746
Size: 81 1411 176 78
Fragments arranged by size:
1411 176 81 78
AfeI AGC'GCT
Cuts at: 0 166 331 1015 1746
Size: 166 165 684 731
Fragments arranged by size:
731 684 166 165
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1003 1035 1746
Size: 1003 32 711
Fragments arranged by size:
1003 711 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1470 1746
Size: 1470 276
ApaI G_GGCC'C
Cuts at: 0 947 1746
Size: 947 799
ApaLI G'TGCA_C
Cuts at: 0 712 1746
Size: 712 1034
ApoI r'AATT_y
Cuts at: 0 801 1132 1453 1746
Size: 801 331 321 293
Fragments arranged by size:
801 331 321 293
AvaI C'yCGr_G
Cuts at: 0 139 739 1746
Size: 139 600 1007
Fragments arranged by size:
1007 600 139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 135 168 1746
Size: 135 33 1578
Fragments arranged by size:
1578 135 33
BamHI G'GATC_C
Cuts at: 0 1373 1746
Size: 1373 373
BanI G'GyrC_C
Cuts at: 0 55 266 483 771 1746
Size: 55 211 217 288 975
Fragments arranged by size:
975 288 217 211 55
BanII G_rGCy'C
Cuts at: 0 740 947 1325 1746
Size: 740 207 378 421
Fragments arranged by size:
740 421 378 207
BbeI G_GCGC'C
Cuts at: 0 270 1746
Size: 270 1476
BbsI GAAGACnn'nnnn_
Cuts at: 0 996 1131 1410 1668 1746
Size: 996 135 279 258 78
Fragments arranged by size:
996 279 258 135 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 555 589 1746
Size: 45 34 476 34 1157
Fragments arranged by size:
1157 476 45 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1048 1746
Size: 1048 698
BclI T'GATC_A
Cuts at: 0 1571 1746
Size: 1571 175
BglI GCCn_nnn'nGGC
Cuts at: 0 769 1746
Size: 769 977
Bme1580I G_kGCm'C
Cuts at: 0 488 716 947 1746
Size: 488 228 231 799
Fragments arranged by size:
799 488 231 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 168 1746
Size: 168 1578
Bpu10I CC'TnA_GC
Cuts at: 0 1324 1746
Size: 1324 422
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 1746
Size: 352 1394
BsaAI yAC'GTr
Cuts at: 0 151 1746
Size: 151 1595
BsaHI Gr'CG_yC
Cuts at: 0 267 1177 1689 1746
Size: 267 910 512 57
Fragments arranged by size:
910 512 267 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1003 1033 1746
Size: 1003 30 713
Fragments arranged by size:
1003 713 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 644 1570 1573 1606 1746
Size: 644 926 3 33 140
Fragments arranged by size:
926 644 140 33 3
BseYI C'CCAG_C
Cuts at: 0 720 1215 1746
Size: 720 495 531
Fragments arranged by size:
720 531 495
BsiEI CG_ry'CG
Cuts at: 0 1540 1746
Size: 1540 206
BsiHKAI G_wGCw'C
Cuts at: 0 716 1325 1746
Size: 716 609 421
Fragments arranged by size:
716 609 421
BsmI GAATG_Cn'
Cuts at: 0 744 1746
Size: 744 1002
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1555 1746
Size: 1555 191
Bsp1286I G_dGCh'C
Cuts at: 0 488 716 740 947 1325 1746
Size: 488 228 24 207 378 421
Fragments arranged by size:
488 421 378 228 207 24
BspHI T'CATG_A
Cuts at: 0 530 1746
Size: 530 1216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 1746
Size: 140 1606
BsrFI r'CCGG_y
Cuts at: 0 1535 1746
Size: 1535 211
BssSI C'ACGA_G
Cuts at: 0 715 1746
Size: 715 1031
BstBI TT'CG_AA
Cuts at: 0 321 1746
Size: 321 1425
BstYI r'GATC_y
Cuts at: 0 678 1373 1746
Size: 678 695 373
Fragments arranged by size:
695 678 373
Bsu36I CC'TnA_GG
Cuts at: 0 1504 1746
Size: 1504 242
BtgI C'CryG_G
Cuts at: 0 555 974 1290 1622 1692 1746
Size: 555 419 316 332 70 54
Fragments arranged by size:
555 419 332 316 70 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 177 1424 1746
Size: 177 1247 322
Fragments arranged by size:
1247 322 177
BtsI GCAGTG_nn'
Cuts at: 0 466 1106 1337 1746
Size: 466 640 231 409
Fragments arranged by size:
640 466 409 231
EaeI y'GGCC_r
Cuts at: 0 188 761 977 1071 1537 1746
Size: 188 573 216 94 466 209
Fragments arranged by size:
573 466 216 209 188 94
EagI C'GGCC_G
Cuts at: 0 1537 1746
Size: 1537 209
EarI CTCTTCn'nnn_
Cuts at: 0 1261 1313 1746
Size: 1261 52 433
Fragments arranged by size:
1261 433 52
EcoICRI GAG'CTC
Cuts at: 0 1323 1746
Size: 1323 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 1492 1668 1746
Size: 81 1411 176 78
Fragments arranged by size:
1411 176 81 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1116 1746
Size: 1116 630
EcoO109I rG'GnC_Cy
Cuts at: 0 144 616 1086 1697 1746
Size: 144 472 470 611 49
Fragments arranged by size:
611 472 470 144 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1299 1746
Size: 1299 447
FseI GG_CCGG'CC
Cuts at: 0 1539 1746
Size: 1539 207
HaeII r_GCGC'y
Cuts at: 0 168 270 333 1017 1746
Size: 168 102 63 684 729
Fragments arranged by size:
729 684 168 102 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 867 899 1162 1194 1746
Size: 867 32 263 32 552
Fragments arranged by size:
867 552 263 32 32
KasI G'GCGC_C
Cuts at: 0 266 1746
Size: 266 1480
MscI TGG'CCA
Cuts at: 0 979 1073 1746
Size: 979 94 673
Fragments arranged by size:
979 673 94
MslI CAynn'nnrTG
Cuts at: 0 749 874 1582 1746
Size: 749 125 708 164
Fragments arranged by size:
749 708 164 125
MspA1I CmG'CkG
Cuts at: 0 187 1746
Size: 187 1559
NaeI GCC'GGC
Cuts at: 0 1537 1746
Size: 1537 209
NarI GG'CG_CC
Cuts at: 0 267 1746
Size: 267 1479
NcoI C'CATG_G
Cuts at: 0 555 1692 1746
Size: 555 1137 54
Fragments arranged by size:
1137 555 54
NgoMIV G'CCGG_C
Cuts at: 0 1535 1746
Size: 1535 211
PasI CC'CwG_GG
Cuts at: 0 732 1746
Size: 732 1014
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 789 1047 1746
Size: 732 57 258 699
Fragments arranged by size:
732 699 258 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 847 879 1003 1035 1746
Size: 847 32 124 32 711
Fragments arranged by size:
847 711 124 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 144 1697 1746
Size: 144 1553 49
Fragments arranged by size:
1553 144 49
PspOMI G'GGCC_C
Cuts at: 0 943 1746
Size: 943 803
PspXI vC'TCGA_Gb
Cuts at: 0 739 1746
Size: 739 1007
SacI G_AGCT'C
Cuts at: 0 1325 1746
Size: 1325 421
SanDI GG'GwC_CC
Cuts at: 0 1697 1746
Size: 1697 49
SapI GCTCTTCn'nnn_
Cuts at: 0 1313 1746
Size: 1313 433
ScaI AGT'ACT
Cuts at: 0 1303 1746
Size: 1303 443
SfcI C'TryA_G
Cuts at: 0 115 1746
Size: 115 1631
SfoI GGC'GCC
Cuts at: 0 268 1746
Size: 268 1478
SmaI CCC'GGG
Cuts at: 0 141 1746
Size: 141 1605
SmlI C'TyrA_G
Cuts at: 0 739 1746
Size: 739 1007
SnaBI TAC'GTA
Cuts at: 0 151 1746
Size: 151 1595
StyI C'CwwG_G
Cuts at: 0 398 555 658 1692 1746
Size: 398 157 103 1034 54
Fragments arranged by size:
1034 398 157 103 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 652 1152 1746
Size: 652 500 594
Fragments arranged by size:
652 594 500
TatI w'GTAC_w
Cuts at: 0 1301 1746
Size: 1301 445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 1746
Size: 458 32 1256
Fragments arranged by size:
1256 458 32
Tth111I GACn'n_nGTC
Cuts at: 0 1741 1746
Size: 1741 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 844 1048 1746
Size: 844 204 698
Fragments arranged by size:
844 698 204
XhoI C'TCGA_G
Cuts at: 0 739 1746
Size: 739 1007
XmaI C'CCGG_G
Cuts at: 0 139 1746
Size: 139 1607
Enzymes that do cut:
AcuI AfeI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI Bme1580I BmrI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrFI BssSI BstBI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PpiI
PpuMI PspOMI PspXI SacI SanDI SapI ScaI SfcI
SfoI SmaI SmlI SnaBI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AscI AseI AsiSI AvrII BbvCI
BfrBI BglII BlpI BmgBI BmtI BplI BpmI BpuEI
BsaBI BsaWI BsgI BsiWI BspEI BsrBI BsrDI BsrGI
BssHII BstAPI BstEII BstXI BstZ17I ClaI CspCI DraI
DraIII DrdI EciI EcoRI EcoRV FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI SgfI SgrAI SpeI
SphI SrfI SspI StuI SwaI XbaI XmnI ZraI