Restriction map for
pF1KB5101
(Linear) (Six-base) MAPSORT of: KIBB5101.seq Check: 8437 from: 1 to: 3147
KIBB5101.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 532 3147
Size: 532 2615
AatII G_ACGT'C
Cuts at: 0 1004 3147
Size: 1004 2143
AccI GT'mk_AC
Cuts at: 0 287 1094 1100 1457 3042 3147
Size: 287 807 6 357 1585 105
Fragments arranged by size:
1585 807 357 287 105 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1621 2911 3147
Size: 609 1012 1290 236
Fragments arranged by size:
1290 1012 609 236
AflIII A'CryG_T
Cuts at: 0 1602 3147
Size: 1602 1545
AhdI GACnn_n'nnGTC
Cuts at: 0 2473 3147
Size: 2473 674
AleI CACnn'nnGTG
Cuts at: 0 2953 3147
Size: 2953 194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 3147
Size: 296 2851
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2374 2406 3147
Size: 2374 32 741
Fragments arranged by size:
2374 741 32
AlwNI CAG_nnn'CTG
Cuts at: 0 699 1326 1335 1719 2101 2556 2724 3147
Size: 699 627 9 384 382 455 168 423
Fragments arranged by size:
699 627 455 423 384 382 168 9
ApaI G_GGCC'C
Cuts at: 0 55 164 1294 2774 3147
Size: 55 109 1130 1480 373
Fragments arranged by size:
1480 1130 373 109 55
ApaLI G'TGCA_C
Cuts at: 0 321 3147
Size: 321 2826
ApoI r'AATT_y
Cuts at: 0 334 554 838 1786 3147
Size: 334 220 284 948 1361
Fragments arranged by size:
1361 948 334 284 220
AvaI C'yCGr_G
Cuts at: 0 156 165 739 1739 2435 2650 2709 2767
Size: 156 9 574 1000 696 215 59 58
Cuts at: 2767 3147
Size: 380
Fragments arranged by size:
1000 696 574 380 215 156 59 58
9
AvrII C'CTAG_G
Cuts at: 0 813 3036 3147
Size: 813 2223 111
Fragments arranged by size:
2223 813 111
BamHI G'GATC_C
Cuts at: 0 2646 3147
Size: 2646 501
BanI G'GyrC_C
Cuts at: 0 59 304 520 1038 2059 3097 3147
Size: 59 245 216 518 1021 1038 50
Fragments arranged by size:
1038 1021 518 245 216 59 50
BanII G_rGCy'C
Cuts at: 0 55 164 172 444 1130 1242 1294 1309
Size: 55 109 8 272 686 112 52 15
Cuts at: 1309 1740 2495 2768 2774 3147
Size: 431 755 273 6 373
Fragments arranged by size:
755 686 431 373 273 272 112 109
55 52 15 8 6
BbeI G_GCGC'C
Cuts at: 0 63 2063 3147
Size: 63 2000 1084
Fragments arranged by size:
2000 1084 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 866 1946 3147
Size: 866 1080 1201
Fragments arranged by size:
1201 1080 866
BbvCI CC'TCA_GC
Cuts at: 0 2302 3147
Size: 2302 845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 137 171 1730 1764 3147
Size: 137 34 1559 34 1383
Fragments arranged by size:
1559 1383 137 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 811 3147
Size: 811 2336
BclI T'GATC_A
Cuts at: 0 749 3147
Size: 749 2398
BglI GCCn_nnn'nGGC
Cuts at: 0 620 1175 1237 2068 3147
Size: 620 555 62 831 1079
Fragments arranged by size:
1079 831 620 555 62
BglII A'GATC_T
Cuts at: 0 1763 1919 3147
Size: 1763 156 1228
Fragments arranged by size:
1763 1228 156
BlpI GC'TnA_GC
Cuts at: 0 2072 3116 3147
Size: 2072 1044 31
Fragments arranged by size:
2072 1044 31
Bme1580I G_kGCm'C
Cuts at: 0 55 164 325 523 881 1041 1294 1680
Size: 55 109 161 198 358 160 253 386
Cuts at: 1680 2774 3147
Size: 1094 373
Fragments arranged by size:
1094 386 373 358 253 198 161 160
109 55
BmgBI CAC'GTC
Cuts at: 0 2334 3147
Size: 2334 813
BmrI ACTGGGnnnn_n'
Cuts at: 0 1843 2410 3147
Size: 1843 567 737
Fragments arranged by size:
1843 737 567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1816 3147
Size: 1816 1331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 580 1677 2078 2124 2659 3113 3147
Size: 382 198 1097 401 46 535 454 34
Fragments arranged by size:
1097 535 454 401 382 198 46 34
Bpu10I CC'TnA_GC
Cuts at: 0 1573 2146 2302 2363 3147
Size: 1573 573 156 61 784
Fragments arranged by size:
1573 784 573 156 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3106 3147
Size: 3106 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 1249 1385 2432 3147
Size: 1249 136 1047 715
Fragments arranged by size:
1249 1047 715 136
BsaBI GATnn'nnATC
Cuts at: 0 1762 1813 3004 3147
Size: 1762 51 1191 143
Fragments arranged by size:
1762 1191 143 51
BsaHI Gr'CG_yC
Cuts at: 0 13 60 1001 2060 2987 3147
Size: 13 47 941 1059 927 160
Fragments arranged by size:
1059 941 927 160 47 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 2076 2106 2374 2404 2728 2758
Size: 394 30 1652 30 268 30 324 30
Cuts at: 2758 3071 3101 3147
Size: 313 30 46
Fragments arranged by size:
1652 394 324 313 268 46 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 441 1397 1540 2470 3147
Size: 406 35 956 143 930 677
Fragments arranged by size:
956 930 677 406 143 35
BseYI C'CCAG_C
Cuts at: 0 47 144 590 1113 1259 1302 2509 3147
Size: 47 97 446 523 146 43 1207 638
Fragments arranged by size:
1207 638 523 446 146 97 47 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 2589 2647 2761 3147
Size: 58 2531 58 114 386
Fragments arranged by size:
2531 386 114 58 58
BsiEI CG_ry'CG
Cuts at: 0 205 3147
Size: 205 2942
BsiHKAI G_wGCw'C
Cuts at: 0 240 325 2495 2768 2849 3147
Size: 240 85 2170 273 81 298
Fragments arranged by size:
2170 298 273 240 85 81
BsmI GAATG_Cn'
Cuts at: 0 2019 3147
Size: 2019 1128
Bsp1286I G_dGCh'C
Cuts at: 0 55 164 172 240 325 444 523 881
Size: 55 109 8 68 85 119 79 358
Cuts at: 881 1041 1130 1242 1294 1309 1680 1740 2495
Size: 160 89 112 52 15 371 60 755
Cuts at: 2495 2768 2774 2849 3147
Size: 273 6 75 298
Fragments arranged by size:
755 371 358 298 273 160 119 112
109 89 85 79 75 68 60 55
52 15 8 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 532 1112 1970 2478 3147
Size: 532 580 858 508 669
Fragments arranged by size:
858 669 580 532 508
BsrBI CCG'CTC
Cuts at: 0 578 1530 3147
Size: 578 952 1617
Fragments arranged by size:
1617 952 578
BsrDI GCAATG_nn'
Cuts at: 0 1410 2965 3147
Size: 1410 1555 182
Fragments arranged by size:
1555 1410 182
BsrFI r'CCGG_y
Cuts at: 0 200 1117 3147
Size: 200 917 2030
Fragments arranged by size:
2030 917 200
BsrGI T'GTAC_A
Cuts at: 0 476 2879 3147
Size: 476 2403 268
Fragments arranged by size:
2403 476 268
BstEII G'GTnAC_C
Cuts at: 0 2082 2373 3147
Size: 2082 291 774
Fragments arranged by size:
2082 774 291
BstYI r'GATC_y
Cuts at: 0 695 1763 1919 2297 2646 2720 3147
Size: 695 1068 156 378 349 74 427
Fragments arranged by size:
1068 695 427 378 349 156 74
BstZ17I GTA'TAC
Cuts at: 0 1458 3147
Size: 1458 1689
Bsu36I CC'TnA_GG
Cuts at: 0 1076 1771 1867 2399 3147
Size: 1076 695 96 532 748
Fragments arranged by size:
1076 748 695 532 96
BtgI C'CryG_G
Cuts at: 0 444 515 1860 2354 3104 3147
Size: 444 71 1345 494 750 43
Fragments arranged by size:
1345 750 494 444 71 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1800 3147
Size: 1800 1347
BtsI GCAGTG_nn'
Cuts at: 0 424 992 2411 2872 3147
Size: 424 568 1419 461 275
Fragments arranged by size:
1419 568 461 424 275
DraIII CAC_nnn'GTG
Cuts at: 0 36 320 3147
Size: 36 284 2827
Fragments arranged by size:
2827 284 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 18 3147
Size: 18 3129
EaeI y'GGCC_r
Cuts at: 0 202 648 989 1152 1167 1229 2945 3147
Size: 202 446 341 163 15 62 1716 202
Fragments arranged by size:
1716 446 341 202 202 163 62 15
EagI C'GGCC_G
Cuts at: 0 202 3147
Size: 202 2945
EcoICRI GAG'CTC
Cuts at: 0 2493 2766 3147
Size: 2493 273 381
Fragments arranged by size:
2493 381 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 580 609 1621 1677 2078 2124 2659
Size: 382 198 29 1012 56 401 46 535
Cuts at: 2659 2911 3113 3147
Size: 252 202 34
Fragments arranged by size:
1012 535 401 382 252 202 198 56
46 34 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 1207 3147
Size: 760 447 1940
Fragments arranged by size:
1940 760 447
EcoO109I rG'GnC_Cy
Cuts at: 0 51 52 141 160 161 1200 1290 1430
Size: 51 1 89 19 1 1039 90 140
Cuts at: 1430 2460 2467 2506 2634 2713 2771 3147
Size: 1030 7 39 128 79 58 376
Fragments arranged by size:
1039 1030 376 140 128 90 89 79
58 51 39 19 7 1 1
EcoRI G'AATT_C
Cuts at: 0 554 838 3147
Size: 554 284 2309
Fragments arranged by size:
2309 554 284
EcoRV GAT'ATC
Cuts at: 0 1411 3147
Size: 1411 1736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2850 3147
Size: 2850 297
HaeII r_GCGC'y
Cuts at: 0 63 2063 3147
Size: 63 2000 1084
Fragments arranged by size:
2000 1084 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 919 951 1786 1818 2459 2491
Size: 394 32 493 32 835 32 641 32
Cuts at: 2491 2705 2737 2781 2813 3147
Size: 214 32 44 32 334
Fragments arranged by size:
835 641 493 394 334 214 44 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 3064 3147
Size: 3064 83
KasI G'GCGC_C
Cuts at: 0 59 2059 3147
Size: 59 2000 1088
Fragments arranged by size:
2000 1088 59
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2624 3147
Size: 2624 523
MscI TGG'CCA
Cuts at: 0 1154 1231 2947 3147
Size: 1154 77 1716 200
Fragments arranged by size:
1716 1154 200 77
MslI CAynn'nnrTG
Cuts at: 0 1951 2486 2953 3103 3147
Size: 1951 535 467 150 44
Fragments arranged by size:
1951 535 467 150 44
MspA1I CmG'CkG
Cuts at: 0 42 72 81 482 590 771 1641 1968
Size: 42 30 9 401 108 181 870 327
Cuts at: 1968 2543 2841 2922 2953 3147
Size: 575 298 81 31 194
Fragments arranged by size:
870 575 401 327 298 194 181 108
81 42 31 30 9
NaeI GCC'GGC
Cuts at: 0 1119 3147
Size: 1119 2028
NarI GG'CG_CC
Cuts at: 0 60 2060 3147
Size: 60 2000 1087
Fragments arranged by size:
2000 1087 60
NcoI C'CATG_G
Cuts at: 0 444 3104 3147
Size: 444 2660 43
Fragments arranged by size:
2660 444 43
NdeI CA'TA_TG
Cuts at: 0 3093 3147
Size: 3093 54
NgoMIV G'CCGG_C
Cuts at: 0 1117 3147
Size: 1117 2030
NspI r_CATG'y
Cuts at: 0 467 3147
Size: 467 2680
PasI CC'CwG_GG
Cuts at: 0 110 1194 2128 3147
Size: 110 1084 934 1019
Fragments arranged by size:
1084 1019 934 110
PflMI CCAn_nnn'nTGG
Cuts at: 0 2046 2228 2556 3147
Size: 2046 182 328 591
Fragments arranged by size:
2046 591 328 182
PfoI T'CCnGG_A
Cuts at: 0 359 2261 3147
Size: 359 1902 886
Fragments arranged by size:
1902 886 359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2374 2406 3147
Size: 2374 32 741
Fragments arranged by size:
2374 741 32
PpuMI rG'GwC_Cy
Cuts at: 0 1200 1430 2460 2467 2713 3147
Size: 1200 230 1030 7 246 434
Fragments arranged by size:
1200 1030 434 246 230 7
PspOMI G'GGCC_C
Cuts at: 0 51 160 1290 2770 3147
Size: 51 109 1130 1480 377
Fragments arranged by size:
1480 1130 377 109 51
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 880 912 1981 2013 3147
Size: 880 32 1069 32 1134
Fragments arranged by size:
1134 1069 880 32 32
PstI C_TGCA'G
Cuts at: 0 275 776 1109 1331 1540 1967 2804 2867
Size: 275 501 333 222 209 427 837 63
Cuts at: 2867 3147
Size: 280
Fragments arranged by size:
837 501 427 333 280 275 222 209
63
PvuII CAG'CTG
Cuts at: 0 42 482 590 771 1641 1968 2543 2922
Size: 42 440 108 181 870 327 575 379
Cuts at: 2922 2953 3147
Size: 31 194
Fragments arranged by size:
870 575 440 379 327 194 181 108
42 31
SacI G_AGCT'C
Cuts at: 0 2495 2768 3147
Size: 2495 273 379
Fragments arranged by size:
2495 379 273
ScaI AGT'ACT
Cuts at: 0 152 2107 3147
Size: 152 1955 1040
Fragments arranged by size:
1955 1040 152
SexAI A'CCwGG_T
Cuts at: 0 755 3147
Size: 755 2392
SfcI C'TryA_G
Cuts at: 0 271 772 1105 1327 1536 1963 2800 2863
Size: 271 501 333 222 209 427 837 63
Cuts at: 2863 3147
Size: 284
Fragments arranged by size:
837 501 427 333 284 271 222 209
63
SfoI GGC'GCC
Cuts at: 0 61 2061 3147
Size: 61 2000 1086
Fragments arranged by size:
2000 1086 61
SmaI CCC'GGG
Cuts at: 0 167 3147
Size: 167 2980
SmlI C'TyrA_G
Cuts at: 0 1739 3121 3147
Size: 1739 1382 26
Fragments arranged by size:
1739 1382 26
SphI G_CATG'C
Cuts at: 0 467 3147
Size: 467 2680
StyI C'CwwG_G
Cuts at: 0 242 298 444 615 813 1832 2502 2966
Size: 242 56 146 171 198 1019 670 464
Cuts at: 2966 3036 3104 3147
Size: 70 68 43
Fragments arranged by size:
1019 670 464 242 198 171 146 70
68 56 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 255 1127 1993 2038 2141 3147
Size: 123 132 872 866 45 103 1006
Fragments arranged by size:
1006 872 866 132 123 103 45
TatI w'GTAC_w
Cuts at: 0 150 404 476 2105 2879 3147
Size: 150 254 72 1629 774 268
Fragments arranged by size:
1629 774 268 254 150 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 173 205 809 841 2076 2108 2728 2760
Size: 173 32 604 32 1235 32 620 32
Cuts at: 2760 3147
Size: 387
Fragments arranged by size:
1235 620 604 387 173 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 217 3147
Size: 217 2930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 479 1162 2423 3147
Size: 479 683 1261 724
Fragments arranged by size:
1261 724 683 479
XhoI C'TCGA_G
Cuts at: 0 1739 3147
Size: 1739 1408
XmaI C'CCGG_G
Cuts at: 0 165 3147
Size: 165 2982
ZraI GAC'GTC
Cuts at: 0 1002 3147
Size: 1002 2145
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI
PsrI PstI PvuII SacI ScaI SexAI SfcI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AscI AseI AsiSI
BaeI BfrBI BmtI BsaAI BsaWI BsiWI BsmBI BspEI
BspHI BssHII BssSI BstAPI BstBI BstXI ClaI CspCI
DraI EarI EciI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PsiI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XmnI