Restriction map for
pF1KB5105
(Linear) (Six-base) MAPSORT of: KIBB5105.seq Check: 1331 from: 1 to: 1851
KIBB5105.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1851
Size: 875 976
AflIII A'CryG_T
Cuts at: 0 368 1851
Size: 368 1483
AleI CACnn'nnGTG
Cuts at: 0 613 1851
Size: 613 1238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1387 1851
Size: 1387 464
AlwNI CAG_nnn'CTG
Cuts at: 0 79 1592 1851
Size: 79 1513 259
Fragments arranged by size:
1513 259 79
ApoI r'AATT_y
Cuts at: 0 583 1442 1753 1851
Size: 583 859 311 98
Fragments arranged by size:
859 583 311 98
BamHI G'GATC_C
Cuts at: 0 1761 1851
Size: 1761 90
BanI G'GyrC_C
Cuts at: 0 1135 1851
Size: 1135 716
BanII G_rGCy'C
Cuts at: 0 881 1851
Size: 881 970
BbsI GAAGACnn'nnnn_
Cuts at: 0 1466 1823 1851
Size: 1466 357 28
Fragments arranged by size:
1466 357 28
BbvCI CC'TCA_GC
Cuts at: 0 1064 1851
Size: 1064 787
BfrBI ATG'CAT
Cuts at: 0 1831 1851
Size: 1831 20
BglI GCCn_nnn'nGGC
Cuts at: 0 1144 1851
Size: 1144 707
Bme1580I G_kGCm'C
Cuts at: 0 1140 1851
Size: 1140 711
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1747 1851
Size: 1747 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 1546 1851
Size: 767 779 305
Fragments arranged by size:
779 767 305
Bpu10I CC'TnA_GC
Cuts at: 0 1064 1436 1851
Size: 1064 372 415
Fragments arranged by size:
1064 415 372
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 1851
Size: 75 1776
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 218 248 353 383 1851
Size: 218 30 105 30 1468
Fragments arranged by size:
1468 218 105 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 1851
Size: 374 1477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 1851
Size: 128 1723
BsiHKAI G_wGCw'C
Cuts at: 0 881 1718 1851
Size: 881 837 133
Fragments arranged by size:
881 837 133
BsmI GAATG_Cn'
Cuts at: 0 1831 1833 1851
Size: 1831 2 18
Fragments arranged by size:
1831 18 2
Bsp1286I G_dGCh'C
Cuts at: 0 881 1140 1718 1851
Size: 881 259 578 133
Fragments arranged by size:
881 578 259 133
BsrDI GCAATG_nn'
Cuts at: 0 533 1851
Size: 533 1318
BsrGI T'GTAC_A
Cuts at: 0 1476 1851
Size: 1476 375
BstYI r'GATC_y
Cuts at: 0 228 1761 1851
Size: 228 1533 90
Fragments arranged by size:
1533 228 90
BtgI C'CryG_G
Cuts at: 0 611 1851
Size: 611 1240
BtsI GCAGTG_nn'
Cuts at: 0 1670 1851
Size: 1670 181
DraIII CAC_nnn'GTG
Cuts at: 0 1240 1851
Size: 1240 611
EaeI y'GGCC_r
Cuts at: 0 687 1851
Size: 687 1164
EarI CTCTTCn'nnn_
Cuts at: 0 913 1851
Size: 913 938
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 461 1851
Size: 461 1390
EcoICRI GAG'CTC
Cuts at: 0 879 1851
Size: 879 972
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 875 1546 1851
Size: 767 108 671 305
Fragments arranged by size:
767 671 305 108
EcoO109I rG'GnC_Cy
Cuts at: 0 76 1068 1851
Size: 76 992 783
Fragments arranged by size:
992 783 76
EcoRV GAT'ATC
Cuts at: 0 324 390 776 1120 1851
Size: 324 66 386 344 731
Fragments arranged by size:
731 386 344 324 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1851
Size: 1036 32 783
Fragments arranged by size:
1036 783 32
HincII GTy'rAC
Cuts at: 0 683 1851
Size: 683 1168
MfeI C'AATT_G
Cuts at: 0 1344 1851
Size: 1344 507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 204 299 1649 1851
Size: 132 72 95 1350 202
Fragments arranged by size:
1350 202 132 95 72
MscI TGG'CCA
Cuts at: 0 689 1851
Size: 689 1162
MslI CAynn'nnrTG
Cuts at: 0 613 1851
Size: 613 1238
NcoI C'CATG_G
Cuts at: 0 611 1851
Size: 611 1240
NsiI A_TGCA'T
Cuts at: 0 1833 1851
Size: 1833 18
NspI r_CATG'y
Cuts at: 0 1824 1851
Size: 1824 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 1240 1851
Size: 1240 611
PfoI T'CCnGG_A
Cuts at: 0 863 1851
Size: 863 988
PpuMI rG'GwC_Cy
Cuts at: 0 76 1851
Size: 76 1775
PshAI GACnn'nnGTC
Cuts at: 0 315 1851
Size: 315 1536
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 623 655 1851
Size: 623 32 1196
Fragments arranged by size:
1196 623 32
PstI C_TGCA'G
Cuts at: 0 1110 1451 1851
Size: 1110 341 400
Fragments arranged by size:
1110 400 341
SacI G_AGCT'C
Cuts at: 0 881 1851
Size: 881 970
ScaI AGT'ACT
Cuts at: 0 1006 1851
Size: 1006 845
SexAI A'CCwGG_T
Cuts at: 0 1236 1851
Size: 1236 615
SfcI C'TryA_G
Cuts at: 0 260 1106 1359 1447 1851
Size: 260 846 253 88 404
Fragments arranged by size:
846 404 260 253 88
StyI C'CwwG_G
Cuts at: 0 611 1379 1851
Size: 611 768 472
Fragments arranged by size:
768 611 472
TatI w'GTAC_w
Cuts at: 0 234 1004 1476 1851
Size: 234 770 472 375
Fragments arranged by size:
770 472 375 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 353 385 755 787 1851
Size: 353 32 370 32 1064
Fragments arranged by size:
1064 370 353 32 32
XbaI T'CTAG_A
Cuts at: 0 1054 1851
Size: 1054 797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 1851
Size: 120 1731
XmnI GAAnn'nnTTC
Cuts at: 0 1494 1831 1851
Size: 1494 337 20
Fragments arranged by size:
1494 337 20
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApoI BamHI BanI
BanII BbsI BbvCI BfrBI BglI Bme1580I BplI BpmI
Bpu10I BsaI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BsrDI BsrGI BstYI BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV Hin4I HincII MfeI
MmeI MscI MslI NcoI NsiI NspI PflMI PfoI
PpuMI PshAI PsrI PstI SacI ScaI SexAI SfcI
StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BclI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PsiI PspOMI PspXI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XhoI XmaI ZraI