Restriction map for
pF1KB5106
(Linear) (Six-base) MAPSORT of: KIBB5106.seq Check: 4697 from: 1 to: 2352
KIBB5106.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1693 2352
Size: 1693 659
AccI GT'mk_AC
Cuts at: 0 593 2352
Size: 593 1759
Acc65I G'GTAC_C
Cuts at: 0 665 2352
Size: 665 1687
AclI AA'CG_TT
Cuts at: 0 2177 2352
Size: 2177 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 836 2224 2352
Size: 343 493 1388 128
Fragments arranged by size:
1388 493 343 128
AflIII A'CryG_T
Cuts at: 0 898 900 1440 2306 2331 2352
Size: 898 2 540 866 25 21
Fragments arranged by size:
898 866 540 25 21 2
AleI CACnn'nnGTG
Cuts at: 0 901 2352
Size: 901 1451
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 2352
Size: 358 1994
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 694 726 2352
Size: 694 32 1626
Fragments arranged by size:
1626 694 32
ApaI G_GGCC'C
Cuts at: 0 24 2352
Size: 24 2328
ApoI r'AATT_y
Cuts at: 0 2128 2352
Size: 2128 224
AvaI C'yCGr_G
Cuts at: 0 17 913 1449 1562 2111 2352
Size: 17 896 536 113 549 241
Fragments arranged by size:
896 549 536 241 113 17
BanI G'GyrC_C
Cuts at: 0 290 538 665 1257 2028 2352
Size: 290 248 127 592 771 324
Fragments arranged by size:
771 592 324 290 248 127
BanII G_rGCy'C
Cuts at: 0 24 34 517 1787 1826 2352
Size: 24 10 483 1270 39 526
Fragments arranged by size:
1270 526 483 39 24 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1885 2352
Size: 1885 467
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 60 94 2352
Size: 60 34 2258
Fragments arranged by size:
2258 60 34
BclI T'GATC_A
Cuts at: 0 1209 1511 2352
Size: 1209 302 841
Fragments arranged by size:
1209 841 302
BglI GCCn_nnn'nGGC
Cuts at: 0 73 1418 2352
Size: 73 1345 934
Fragments arranged by size:
1345 934 73
BlpI GC'TnA_GC
Cuts at: 0 1290 2352
Size: 1290 1062
Bme1580I G_kGCm'C
Cuts at: 0 24 783 920 1260 2031 2352
Size: 24 759 137 340 771 321
Fragments arranged by size:
771 759 340 321 137 24
BmgBI CAC'GTC
Cuts at: 0 2307 2352
Size: 2307 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 243 714 2352
Size: 243 471 1638
Fragments arranged by size:
1638 471 243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 75 261 2352
Size: 75 186 2091
Fragments arranged by size:
2091 186 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 231 454 661 1567 2352
Size: 70 161 223 207 906 785
Fragments arranged by size:
906 785 223 207 161 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 2086 2239 2352
Size: 92 1994 153 113
Fragments arranged by size:
1994 153 113 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 1735 2352
Size: 1735 617
BsaAI yAC'GTr
Cuts at: 0 156 901 921 2352
Size: 156 745 20 1431
Fragments arranged by size:
1431 745 156 20
BsaHI Gr'CG_yC
Cuts at: 0 596 2352
Size: 596 1756
BsaWI w'CCGG_w
Cuts at: 0 410 2352
Size: 410 1942
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 739 769 1430 1460 2352
Size: 739 30 661 30 892
Fragments arranged by size:
892 739 661 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 760 944 1134 1167 1591 2352
Size: 451 309 184 190 33 424 761
Fragments arranged by size:
761 451 424 309 190 184 33
BseYI C'CCAG_C
Cuts at: 0 740 885 1020 1267 2004 2157 2352
Size: 740 145 135 247 737 153 195
Fragments arranged by size:
740 737 247 195 153 145 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 556 943 1102 1120 2352
Size: 496 60 387 159 18 1232
Fragments arranged by size:
1232 496 387 159 60 18
BsiEI CG_ry'CG
Cuts at: 0 1283 1692 1793 2352
Size: 1283 409 101 559
Fragments arranged by size:
1283 559 409 101
BsiHKAI G_wGCw'C
Cuts at: 0 56 1862 1907 2228 2352
Size: 56 1806 45 321 124
Fragments arranged by size:
1806 321 124 56 45
BsiWI C'GTAC_G
Cuts at: 0 1284 2352
Size: 1284 1068
BsmI GAATG_Cn'
Cuts at: 0 946 2352
Size: 946 1406
Bsp1286I G_dGCh'C
Cuts at: 0 24 34 56 517 783 920 1260 1787
Size: 24 10 22 461 266 137 340 527
Cuts at: 1787 1826 1862 1907 2031 2228 2352
Size: 39 36 45 124 197 124
Fragments arranged by size:
527 461 340 266 197 137 124 124
45 39 36 24 22 10
BspEI T'CCGG_A
Cuts at: 0 410 2352
Size: 410 1942
BspHI T'CATG_A
Cuts at: 0 602 2270 2352
Size: 602 1668 82
Fragments arranged by size:
1668 602 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 347 1693 2352
Size: 347 1346 659
Fragments arranged by size:
1346 659 347
BsrDI GCAATG_nn'
Cuts at: 0 1307 2352
Size: 1307 1045
BsrFI r'CCGG_y
Cuts at: 0 67 1408 2352
Size: 67 1341 944
Fragments arranged by size:
1341 944 67
BssSI C'ACGA_G
Cuts at: 0 1573 2352
Size: 1573 779
BstBI TT'CG_AA
Cuts at: 0 1817 2352
Size: 1817 535
BstXI CCAn_nnnn'nTGG
Cuts at: 0 972 1599 2079 2352
Size: 972 627 480 273
Fragments arranged by size:
972 627 480 273
BstYI r'GATC_y
Cuts at: 0 956 1214 1839 2352
Size: 956 258 625 513
Fragments arranged by size:
956 625 513 258
BtgI C'CryG_G
Cuts at: 0 134 608 1262 1592 1745 2352
Size: 134 474 654 330 153 607
Fragments arranged by size:
654 607 474 330 153 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 204 975 1123 2352
Size: 204 771 148 1229
Fragments arranged by size:
1229 771 204 148
BtsI GCAGTG_nn'
Cuts at: 0 288 1929 2090 2352
Size: 288 1641 161 262
Fragments arranged by size:
1641 288 262 161
ClaI AT'CG_AT
Cuts at: 0 461 2352
Size: 461 1891
DraIII CAC_nnn'GTG
Cuts at: 0 1056 2352
Size: 1056 1296
EaeI y'GGCC_r
Cuts at: 0 443 453 962 1280 1317 1410 1769 1790
Size: 443 10 509 318 37 93 359 21
Cuts at: 1790 2282 2352
Size: 492 70
Fragments arranged by size:
509 492 443 359 318 93 70 37
21 10
EagI C'GGCC_G
Cuts at: 0 1280 1790 2352
Size: 1280 510 562
Fragments arranged by size:
1280 562 510
EarI CTCTTCn'nnn_
Cuts at: 0 412 1460 1814 1961 2352
Size: 412 1048 354 147 391
Fragments arranged by size:
1048 412 391 354 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 555 1695 2352
Size: 555 1140 657
Fragments arranged by size:
1140 657 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 231 343 454 661 836 1567 2224
Size: 70 161 112 111 207 175 731 657
Cuts at: 2224 2352
Size: 128
Fragments arranged by size:
731 657 207 175 161 128 112 111
70
EcoO109I rG'GnC_Cy
Cuts at: 0 21 629 935 2352
Size: 21 608 306 1417
Fragments arranged by size:
1417 608 306 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 51 189 2352
Size: 51 138 2163
Fragments arranged by size:
2163 138 51
FspI TGC'GCA
Cuts at: 0 142 1486 2352
Size: 142 1344 866
Fragments arranged by size:
1344 866 142
FspAI rTGC'GCAy
Cuts at: 0 142 1486 2352
Size: 142 1344 866
Fragments arranged by size:
1344 866 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 1178 1210 1831 1863 2352
Size: 196 32 950 32 621 32 489
Fragments arranged by size:
950 621 489 196 32 32 32
HincII GTy'rAC
Cuts at: 0 594 2352
Size: 594 1758
KpnI G_GTAC'C
Cuts at: 0 669 2352
Size: 669 1683
MfeI C'AATT_G
Cuts at: 0 824 1227 2352
Size: 824 403 1125
Fragments arranged by size:
1125 824 403
MluI A'CGCG_T
Cuts at: 0 1440 2352
Size: 1440 912
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1643 1874 2291 2352
Size: 1643 231 417 61
Fragments arranged by size:
1643 417 231 61
MscI TGG'CCA
Cuts at: 0 445 964 1771 2284 2352
Size: 445 519 807 513 68
Fragments arranged by size:
807 519 513 445 68
MslI CAynn'nnrTG
Cuts at: 0 82 544 901 976 1249 1480 1597 2152
Size: 82 462 357 75 273 231 117 555
Cuts at: 2152 2352
Size: 200
Fragments arranged by size:
555 462 357 273 231 200 117 82
75
MspA1I CmG'CkG
Cuts at: 0 95 885 1068 1264 1294 1712 1956 2325
Size: 95 790 183 196 30 418 244 369
Cuts at: 2325 2352
Size: 27
Fragments arranged by size:
790 418 369 244 196 183 95 30
27
NaeI GCC'GGC
Cuts at: 0 69 2352
Size: 69 2283
NcoI C'CATG_G
Cuts at: 0 1592 1745 2352
Size: 1592 153 607
Fragments arranged by size:
1592 607 153
NgoMIV G'CCGG_C
Cuts at: 0 67 2352
Size: 67 2285
NspI r_CATG'y
Cuts at: 0 2016 2335 2352
Size: 2016 319 17
Fragments arranged by size:
2016 319 17
PciI A'CATG_T
Cuts at: 0 2331 2352
Size: 2331 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 198 2352
Size: 198 2154
PfoI T'CCnGG_A
Cuts at: 0 47 1544 2352
Size: 47 1497 808
Fragments arranged by size:
1497 808 47
PmlI CAC'GTG
Cuts at: 0 901 921 2352
Size: 901 20 1431
Fragments arranged by size:
1431 901 20
PpuMI rG'GwC_Cy
Cuts at: 0 629 935 2352
Size: 629 306 1417
Fragments arranged by size:
1417 629 306
PshAI GACnn'nnGTC
Cuts at: 0 1393 2352
Size: 1393 959
PspOMI G'GGCC_C
Cuts at: 0 20 2352
Size: 20 2332
PstI C_TGCA'G
Cuts at: 0 347 1847 1924 1993 2352
Size: 347 1500 77 69 359
Fragments arranged by size:
1500 359 347 77 69
PvuII CAG'CTG
Cuts at: 0 885 1294 1956 2325 2352
Size: 885 409 662 369 27
Fragments arranged by size:
885 662 409 369 27
SacII CC_GC'GG
Cuts at: 0 1265 2352
Size: 1265 1087
SalI G'TCGA_C
Cuts at: 0 592 2352
Size: 592 1760
SanDI GG'GwC_CC
Cuts at: 0 935 2352
Size: 935 1417
SapI GCTCTTCn'nnn_
Cuts at: 0 1814 2352
Size: 1814 538
ScaI AGT'ACT
Cuts at: 0 1459 2352
Size: 1459 893
SfcI C'TryA_G
Cuts at: 0 343 1843 1920 1989 2352
Size: 343 1500 77 69 363
Fragments arranged by size:
1500 363 343 77 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 73 2352
Size: 73 2279
SmaI CCC'GGG
Cuts at: 0 19 1451 1564 2113 2352
Size: 19 1432 113 549 239
Fragments arranged by size:
1432 549 239 113 19
SmlI C'TyrA_G
Cuts at: 0 107 2101 2254 2352
Size: 107 1994 153 98
Fragments arranged by size:
1994 153 107 98
SspI AAT'ATT
Cuts at: 0 801 2352
Size: 801 1551
StuI AGG'CCT
Cuts at: 0 298 1531 1898 2352
Size: 298 1233 367 454
Fragments arranged by size:
1233 454 367 298
StyI C'CwwG_G
Cuts at: 0 26 164 686 1361 1413 1592 1745 2352
Size: 26 138 522 675 52 179 153 607
Fragments arranged by size:
675 607 522 179 153 138 52 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 696 2352
Size: 696 1656
TatI w'GTAC_w
Cuts at: 0 170 1457 2288 2352
Size: 170 1287 831 64
Fragments arranged by size:
1287 831 170 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 2352
Size: 739 32 1581
Fragments arranged by size:
1581 739 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 737 2154 2352
Size: 737 1417 198
Fragments arranged by size:
1417 737 198
XmaI C'CCGG_G
Cuts at: 0 17 1449 1562 2111 2352
Size: 17 1432 113 549 241
Fragments arranged by size:
1432 549 241 113 17
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI ApaI ApoI AvaI BanI BanII BbsI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraIII EaeI EagI EarI EciI
Eco57MI EcoO109I FalI FspI FspAI Hin4I HincII KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PciI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacII SalI SanDI SapI ScaI
SfcI SfiI SmaI SmlI SspI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglII BmtI Bpu10I BsaBI BsmBI BsrBI BsrGI
BssHII BstAPI BstEII BstZ17I Bsu36I CspCI DraI DrdI
EcoICRI EcoNI EcoRI EcoRV FseI HaeII HindIII HpaI
KasI NarI NdeI NheI NotI NruI NsiI PacI
PasI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmnI ZraI