Restriction map for
pF1KB5109
(Linear) (Six-base) MAPSORT of: KIBB5109.seq Check: 9142 from: 1 to: 1818
KIBB5109.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 30 881 1818
Size: 30 851 937
Fragments arranged by size:
937 851 30
AatII G_ACGT'C
Cuts at: 0 788 1818
Size: 788 1030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1299 1315 1456 1818
Size: 353 946 16 141 362
Fragments arranged by size:
946 362 353 141 16
AhdI GACnn_n'nnGTC
Cuts at: 0 1285 1818
Size: 1285 533
AleI CACnn'nnGTG
Cuts at: 0 701 1818
Size: 701 1117
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 760 952 1818
Size: 760 192 866
Fragments arranged by size:
866 760 192
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 1818
Size: 1087 32 699
Fragments arranged by size:
1087 699 32
AlwNI CAG_nnn'CTG
Cuts at: 0 846 975 1818
Size: 846 129 843
Fragments arranged by size:
846 843 129
ApaI G_GGCC'C
Cuts at: 0 1471 1818
Size: 1471 347
ApaLI G'TGCA_C
Cuts at: 0 1357 1818
Size: 1357 461
AseI AT'TA_AT
Cuts at: 0 1037 1818
Size: 1037 781
AvaI C'yCGr_G
Cuts at: 0 1249 1818
Size: 1249 569
BanI G'GyrC_C
Cuts at: 0 1426 1501 1818
Size: 1426 75 317
Fragments arranged by size:
1426 317 75
BanII G_rGCy'C
Cuts at: 0 826 1343 1471 1818
Size: 826 517 128 347
Fragments arranged by size:
826 517 347 128
BbeI G_GCGC'C
Cuts at: 0 1505 1818
Size: 1505 313
BbsI GAAGACnn'nnnn_
Cuts at: 0 341 381 661 1818
Size: 341 40 280 1157
Fragments arranged by size:
1157 341 280 40
BbvCI CC'TCA_GC
Cuts at: 0 244 1818
Size: 244 1574
BciVI GTATCCnnnnn_n'
Cuts at: 0 249 1249 1818
Size: 249 1000 569
Fragments arranged by size:
1000 569 249
BglI GCCn_nnn'nGGC
Cuts at: 0 480 1818
Size: 480 1338
Bme1580I G_kGCm'C
Cuts at: 0 1361 1429 1471 1818
Size: 1361 68 42 347
Fragments arranged by size:
1361 347 68 42
BmgBI CAC'GTC
Cuts at: 0 942 1482 1818
Size: 942 540 336
Fragments arranged by size:
942 540 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 668 1053 1818
Size: 668 385 765
Fragments arranged by size:
765 668 385
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1086 1818
Size: 1086 732
Bpu10I CC'TnA_GC
Cuts at: 0 244 1818
Size: 244 1574
BsaBI GATnn'nnATC
Cuts at: 0 640 1818
Size: 640 1178
BsaHI Gr'CG_yC
Cuts at: 0 53 785 1502 1523 1818
Size: 53 732 717 21 295
Fragments arranged by size:
732 717 295 53 21
BsaWI w'CCGG_w
Cuts at: 0 223 1645 1818
Size: 223 1422 173
Fragments arranged by size:
1422 223 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 262 292 655 685 1627 1657
Size: 205 30 27 30 363 30 942 30
Cuts at: 1657 1818
Size: 161
Fragments arranged by size:
942 363 205 161 30 30 30 30
27
BseYI C'CCAG_C
Cuts at: 0 141 202 304 678 846 925 1078 1818
Size: 141 61 102 374 168 79 153 740
Fragments arranged by size:
740 374 168 153 141 102 79 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1711 1818
Size: 922 789 107
Fragments arranged by size:
922 789 107
BsiHKAI G_wGCw'C
Cuts at: 0 170 275 1361 1763 1818
Size: 170 105 1086 402 55
Fragments arranged by size:
1086 402 170 105 55
BsiWI C'GTAC_G
Cuts at: 0 5 1818
Size: 5 1813
BsmI GAATG_Cn'
Cuts at: 0 514 1818
Size: 514 1304
BsmBI CGTCTCn'nnnn_
Cuts at: 0 60 1048 1818
Size: 60 988 770
Fragments arranged by size:
988 770 60
Bsp1286I G_dGCh'C
Cuts at: 0 170 275 826 1343 1361 1429 1471 1763
Size: 170 105 551 517 18 68 42 292
Cuts at: 1763 1818
Size: 55
Fragments arranged by size:
551 517 292 170 105 68 55 42
18
BspEI T'CCGG_A
Cuts at: 0 223 1818
Size: 223 1595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 30 881 1818
Size: 30 851 937
Fragments arranged by size:
937 851 30
BsrDI GCAATG_nn'
Cuts at: 0 215 320 1818
Size: 215 105 1498
Fragments arranged by size:
1498 215 105
BsrFI r'CCGG_y
Cuts at: 0 470 479 1721 1818
Size: 470 9 1242 97
Fragments arranged by size:
1242 470 97 9
BsrGI T'GTAC_A
Cuts at: 0 98 1818
Size: 98 1720
BssHII G'CGCG_C
Cuts at: 0 674 1818
Size: 674 1144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 703 834 1533 1818
Size: 703 131 699 285
Fragments arranged by size:
703 699 285 131
BstYI r'GATC_y
Cuts at: 0 248 1706 1818
Size: 248 1458 112
Fragments arranged by size:
1458 248 112
Bsu36I CC'TnA_GG
Cuts at: 0 80 124 1818
Size: 80 44 1694
Fragments arranged by size:
1694 80 44
BtgI C'CryG_G
Cuts at: 0 353 488 800 818 827 1115 1262 1818
Size: 353 135 312 18 9 288 147 556
Fragments arranged by size:
556 353 312 288 147 135 18 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 512 1818
Size: 512 1306
BtsI GCAGTG_nn'
Cuts at: 0 1361 1818
Size: 1361 457
ClaI AT'CG_AT
Cuts at: 0 964 1818
Size: 964 854
DrdI GACnn_nn'nnGTC
Cuts at: 0 1402 1818
Size: 1402 416
EaeI y'GGCC_r
Cuts at: 0 188 472 485 630 1818
Size: 188 284 13 145 1188
Fragments arranged by size:
1188 284 188 145 13
EarI CTCTTCn'nnn_
Cuts at: 0 414 1818
Size: 414 1404
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 698 1396 1818
Size: 698 698 422
Fragments arranged by size:
698 698 422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1299 1315 1456 1818
Size: 353 946 16 141 362
Fragments arranged by size:
946 362 353 141 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 1294 1818
Size: 37 1257 524
Fragments arranged by size:
1257 524 37
EcoO109I rG'GnC_Cy
Cuts at: 0 526 1467 1468 1818
Size: 526 941 1 350
Fragments arranged by size:
941 526 350 1
HaeII r_GCGC'y
Cuts at: 0 1505 1818
Size: 1505 313
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 957 989 1271 1303 1549 1581
Size: 391 32 534 32 282 32 246 32
Cuts at: 1581 1691 1723 1818
Size: 110 32 95
Fragments arranged by size:
534 391 282 246 110 95 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 576 1818
Size: 576 1242
KasI G'GCGC_C
Cuts at: 0 1501 1818
Size: 1501 317
MfeI C'AATT_G
Cuts at: 0 1382 1818
Size: 1382 436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1818
Size: 703 1115
MscI TGG'CCA
Cuts at: 0 190 487 1818
Size: 190 297 1331
Fragments arranged by size:
1331 297 190
MslI CAynn'nnrTG
Cuts at: 0 701 1657 1663 1818
Size: 701 956 6 155
Fragments arranged by size:
956 701 155 6
MspA1I CmG'CkG
Cuts at: 0 206 846 1142 1196 1818
Size: 206 640 296 54 622
Fragments arranged by size:
640 622 296 206 54
NaeI GCC'GGC
Cuts at: 0 472 1818
Size: 472 1346
NarI GG'CG_CC
Cuts at: 0 1502 1818
Size: 1502 316
NcoI C'CATG_G
Cuts at: 0 818 1818
Size: 818 1000
NgoMIV G'CCGG_C
Cuts at: 0 470 1818
Size: 470 1348
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 779 846 1818
Size: 180 599 67 972
Fragments arranged by size:
972 599 180 67
PfoI T'CCnGG_A
Cuts at: 0 1105 1818
Size: 1105 713
PpuMI rG'GwC_Cy
Cuts at: 0 526 1818
Size: 526 1292
PspOMI G'GGCC_C
Cuts at: 0 1467 1818
Size: 1467 351
PstI C_TGCA'G
Cuts at: 0 1147 1818
Size: 1147 671
PvuII CAG'CTG
Cuts at: 0 846 1142 1818
Size: 846 296 676
Fragments arranged by size:
846 676 296
SanDI GG'GwC_CC
Cuts at: 0 526 1818
Size: 526 1292
SapI GCTCTTCn'nnn_
Cuts at: 0 414 1818
Size: 414 1404
SexAI A'CCwGG_T
Cuts at: 0 1097 1818
Size: 1097 721
SfcI C'TryA_G
Cuts at: 0 1143 1191 1818
Size: 1143 48 627
Fragments arranged by size:
1143 627 48
SfoI GGC'GCC
Cuts at: 0 1503 1818
Size: 1503 315
SmaI CCC'GGG
Cuts at: 0 1251 1818
Size: 1251 567
StyI C'CwwG_G
Cuts at: 0 818 1463 1818
Size: 818 645 355
Fragments arranged by size:
818 645 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 754 1492 1818
Size: 754 738 326
Fragments arranged by size:
754 738 326
TatI w'GTAC_w
Cuts at: 0 98 1818
Size: 98 1720
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 655 687 1247 1279 1627 1659 1668 1700
Size: 655 32 560 32 348 32 9 32
Cuts at: 1700 1818
Size: 118
Fragments arranged by size:
655 560 348 118 32 32 32 32
9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 1113 1818
Size: 301 812 705
Fragments arranged by size:
812 705 301
XmaI C'CCGG_G
Cuts at: 0 1249 1818
Size: 1249 569
XmnI GAAnn'nnTTC
Cuts at: 0 22 1312 1818
Size: 22 1290 506
Fragments arranged by size:
1290 506 22
ZraI GAC'GTC
Cuts at: 0 786 1818
Size: 786 1032
Enzymes that do cut:
AarI AatII AcuI AhdI AleI AlfI AloI AlwNI
ApaI ApaLI AseI AvaI BanI BanII BbeI BbsI
BbvCI BciVI BglI Bme1580I BmgBI BmrI BplI Bpu10I
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI
BssHII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PflMI PfoI PpuMI PspOMI
PstI PvuII SanDI SapI SexAI SfcI SfoI SmaI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI ApoI
AscI AsiSI AvrII BaeI BamHI BcgI BclI BfrBI
BglII BlpI BmtI BpmI BpuEI BsaI BsaAI BseRI
BsiEI BspHI BsrBI BssSI BstAPI BstBI BstEII BstZ17I
CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MluI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SfiI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI