Restriction map for
pF1KB5110
(Linear) (Six-base) MAPSORT of: KIBB5110.seq Check: 1002 from: 1 to: 1914
KIBB5110.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 236 1208 1914
Size: 236 972 706
Fragments arranged by size:
972 706 236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 991 1323 1914
Size: 853 138 332 591
Fragments arranged by size:
853 591 332 138
AflIII A'CryG_T
Cuts at: 0 89 1914
Size: 89 1825
AleI CACnn'nnGTG
Cuts at: 0 1456 1914
Size: 1456 458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1474 1598 1914
Size: 1474 124 316
Fragments arranged by size:
1474 316 124
AlwNI CAG_nnn'CTG
Cuts at: 0 562 876 1188 1914
Size: 562 314 312 726
Fragments arranged by size:
726 562 314 312
ApoI r'AATT_y
Cuts at: 0 412 1765 1914
Size: 412 1353 149
Fragments arranged by size:
1353 412 149
AvaI C'yCGr_G
Cuts at: 0 734 1914
Size: 734 1180
BanI G'GyrC_C
Cuts at: 0 1843 1914
Size: 1843 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 571 1013 1914
Size: 571 442 901
Fragments arranged by size:
901 571 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 889 923 1812 1846 1914
Size: 889 34 889 34 68
Fragments arranged by size:
889 889 68 34 34
BclI T'GATC_A
Cuts at: 0 1038 1914
Size: 1038 876
Bme1580I G_kGCm'C
Cuts at: 0 1422 1846 1914
Size: 1422 424 68
Fragments arranged by size:
1422 424 68
BmgBI CAC'GTC
Cuts at: 0 255 1545 1914
Size: 255 1290 369
Fragments arranged by size:
1290 369 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 757 860 958 1914
Size: 757 103 98 956
Fragments arranged by size:
956 757 103 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1415 1914
Size: 1415 499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 1017 1914
Size: 145 872 897
Fragments arranged by size:
897 872 145
BsaAI yAC'GTr
Cuts at: 0 1528 1914
Size: 1528 386
BsaWI w'CCGG_w
Cuts at: 0 76 1914
Size: 76 1838
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 634 658 1914
Size: 388 246 24 1256
Fragments arranged by size:
1256 388 246 24
BseYI C'CCAG_C
Cuts at: 0 1235 1375 1914
Size: 1235 140 539
Fragments arranged by size:
1235 539 140
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1480 1914
Size: 1201 279 434
Fragments arranged by size:
1201 434 279
BsiHKAI G_wGCw'C
Cuts at: 0 44 396 1914
Size: 44 352 1518
Fragments arranged by size:
1518 352 44
Bsp1286I G_dGCh'C
Cuts at: 0 44 396 1422 1846 1914
Size: 44 352 1026 424 68
Fragments arranged by size:
1026 424 352 68 44
BspHI T'CATG_A
Cuts at: 0 1904 1914
Size: 1904 10
BsrDI GCAATG_nn'
Cuts at: 0 1046 1070 1349 1499 1514 1674 1914
Size: 1046 24 279 150 15 160 240
Fragments arranged by size:
1046 279 240 160 150 24 15
BsrFI r'CCGG_y
Cuts at: 0 1846 1914
Size: 1846 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1072 1188 1914
Size: 1072 116 726
Fragments arranged by size:
1072 726 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1107 1890 1914
Size: 1107 783 24
Fragments arranged by size:
1107 783 24
BstYI r'GATC_y
Cuts at: 0 215 290 386 873 1914
Size: 215 75 96 487 1041
Fragments arranged by size:
1041 487 215 96 75
Bsu36I CC'TnA_GG
Cuts at: 0 809 1914
Size: 809 1105
BtgI C'CryG_G
Cuts at: 0 1382 1454 1505 1676 1914
Size: 1382 72 51 171 238
Fragments arranged by size:
1382 238 171 72 51
DraIII CAC_nnn'GTG
Cuts at: 0 952 1914
Size: 952 962
DrdI GACnn_nn'nnGTC
Cuts at: 0 1075 1687 1914
Size: 1075 612 227
Fragments arranged by size:
1075 612 227
EaeI y'GGCC_r
Cuts at: 0 1446 1604 1848 1914
Size: 1446 158 244 66
Fragments arranged by size:
1446 244 158 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 991 1323 1415 1914
Size: 853 138 332 92 499
Fragments arranged by size:
853 499 332 138 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1906 1914
Size: 1906 8
EcoO109I rG'GnC_Cy
Cuts at: 0 589 975 1914
Size: 589 386 939
Fragments arranged by size:
939 589 386
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1332 1914
Size: 1332 582
HaeII r_GCGC'y
Cuts at: 0 1251 1914
Size: 1251 663
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 378 410 984 1000 1016 1023 1032 1055
Size: 378 32 574 16 16 7 9 23
Cuts at: 1055 1914
Size: 859
Fragments arranged by size:
859 574 378 32 23 16 16 9
7
HincII GTy'rAC
Cuts at: 0 87 1914
Size: 87 1827
HpaI GTT'AAC
Cuts at: 0 87 1914
Size: 87 1827
MluI A'CGCG_T
Cuts at: 0 89 1914
Size: 89 1825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 707 755 1088 1914
Size: 318 389 48 333 826
Fragments arranged by size:
826 389 333 318 48
MscI TGG'CCA
Cuts at: 0 1606 1914
Size: 1606 308
MslI CAynn'nnrTG
Cuts at: 0 403 1456 1504 1914
Size: 403 1053 48 410
Fragments arranged by size:
1053 410 403 48
MspA1I CmG'CkG
Cuts at: 0 1392 1686 1914
Size: 1392 294 228
Fragments arranged by size:
1392 294 228
NcoI C'CATG_G
Cuts at: 0 1454 1505 1914
Size: 1454 51 409
Fragments arranged by size:
1454 409 51
NdeI CA'TA_TG
Cuts at: 0 231 1914
Size: 231 1683
NspI r_CATG'y
Cuts at: 0 960 1215 1575 1914
Size: 960 255 360 339
Fragments arranged by size:
960 360 339 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 1722 1914
Size: 1722 192
PfoI T'CCnGG_A
Cuts at: 0 1217 1914
Size: 1217 697
PpuMI rG'GwC_Cy
Cuts at: 0 589 1914
Size: 589 1325
PstI C_TGCA'G
Cuts at: 0 116 567 886 1068 1193 1914
Size: 116 451 319 182 125 721
Fragments arranged by size:
721 451 319 182 125 116
PvuII CAG'CTG
Cuts at: 0 1392 1686 1914
Size: 1392 294 228
Fragments arranged by size:
1392 294 228
ScaI AGT'ACT
Cuts at: 0 786 1914
Size: 786 1128
SfcI C'TryA_G
Cuts at: 0 112 563 882 1064 1165 1189 1914
Size: 112 451 319 182 101 24 725
Fragments arranged by size:
725 451 319 182 112 101 24
SmaI CCC'GGG
Cuts at: 0 736 1914
Size: 736 1178
SmlI C'TyrA_G
Cuts at: 0 160 996 1914
Size: 160 836 918
Fragments arranged by size:
918 836 160
SphI G_CATG'C
Cuts at: 0 1575 1914
Size: 1575 339
StyI C'CwwG_G
Cuts at: 0 26 479 938 1454 1505 1533 1706 1883
Size: 26 453 459 516 51 28 173 177
Cuts at: 1883 1914
Size: 31
Fragments arranged by size:
516 459 453 177 173 51 31 28
26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 157 739 940 1914
Size: 157 582 201 974
Fragments arranged by size:
974 582 201 157
TatI w'GTAC_w
Cuts at: 0 784 1914
Size: 784 1130
Tth111I GACn'n_nGTC
Cuts at: 0 852 1914
Size: 852 1062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 945 1836 1890 1914
Size: 945 891 54 24
Fragments arranged by size:
945 891 54 24
XmaI C'CCGG_G
Cuts at: 0 734 1914
Size: 734 1180
XmnI GAAnn'nnTTC
Cuts at: 0 1336 1914
Size: 1336 578
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AvaI
BanI BbsI BcgI BclI Bme1580I BmgBI BmrI BpmI
BpuEI BsaAI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
DraIII DrdI EaeI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HpaI MluI MmeI MscI MslI MspA1I
NcoI NdeI NspI PflMI PfoI PpuMI PstI PvuII
ScaI SfcI SmaI SmlI SphI StyI TaqII TatI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BciVI BfrBI BglI BglII
BlpI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI EagI EarI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII KasI KpnI MfeI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TstI XbaI XhoI ZraI