Restriction map for
pF1KB5115
(Linear) (Six-base) MAPSORT of: KIBB5115.seq Check: 5688 from: 1 to: 3291
KIBB5115.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2154 3291
Size: 2154 1137
AccI GT'mk_AC
Cuts at: 0 740 824 3291
Size: 740 84 2467
Fragments arranged by size:
2467 740 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 2665 2912 3291
Size: 530 2135 247 379
Fragments arranged by size:
2135 530 379 247
AflIII A'CryG_T
Cuts at: 0 86 829 1134 1146 3065 3291
Size: 86 743 305 12 1919 226
Fragments arranged by size:
1919 743 305 226 86 12
AhdI GACnn_n'nnGTC
Cuts at: 0 3066 3291
Size: 3066 225
AleI CACnn'nnGTG
Cuts at: 0 769 3291
Size: 769 2522
AlwNI CAG_nnn'CTG
Cuts at: 0 185 508 3291
Size: 185 323 2783
Fragments arranged by size:
2783 323 185
ApaI G_GGCC'C
Cuts at: 0 643 1874 3291
Size: 643 1231 1417
Fragments arranged by size:
1417 1231 643
ApoI r'AATT_y
Cuts at: 0 553 1534 2610 2886 3291
Size: 553 981 1076 276 405
Fragments arranged by size:
1076 981 553 405 276
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 732 765 3291
Size: 732 33 2526
Fragments arranged by size:
2526 732 33
BanI G'GyrC_C
Cuts at: 0 1921 2029 3291
Size: 1921 108 1262
Fragments arranged by size:
1921 1262 108
BanII G_rGCy'C
Cuts at: 0 460 643 1874 3105 3291
Size: 460 183 1231 1231 186
Fragments arranged by size:
1231 1231 460 186 183
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 469 952 3291
Size: 17 452 483 2339
Fragments arranged by size:
2339 483 452 17
BbvCI CC'TCA_GC
Cuts at: 0 131 362 3291
Size: 131 231 2929
Fragments arranged by size:
2929 231 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3085 3119 3291
Size: 3085 34 172
Fragments arranged by size:
3085 172 34
BclI T'GATC_A
Cuts at: 0 684 1002 2853 3291
Size: 684 318 1851 438
Fragments arranged by size:
1851 684 438 318
BglII A'GATC_T
Cuts at: 0 414 3291
Size: 414 2877
Bme1580I G_kGCm'C
Cuts at: 0 643 1874 1926 2034 2493 3291
Size: 643 1231 52 108 459 798
Fragments arranged by size:
1231 798 643 459 108 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 173 3291
Size: 173 3118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2595 2890 3291
Size: 2595 295 401
Fragments arranged by size:
2595 401 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 374 455 807 3291
Size: 50 324 81 352 2484
Fragments arranged by size:
2484 352 324 81 50
Bpu10I CC'TnA_GC
Cuts at: 0 131 362 3167 3291
Size: 131 231 2805 124
Fragments arranged by size:
2805 231 131 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 3263 3291
Size: 918 2345 28
Fragments arranged by size:
2345 918 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 2627 3291
Size: 2627 664
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3164 3194 3291
Size: 3164 30 97
Fragments arranged by size:
3164 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 47 50 1711 1775 1820 2054 2192 2240
Size: 47 3 1661 64 45 234 138 48
Cuts at: 2240 2288 2336 2384 2432 3291
Size: 48 48 48 48 859
Fragments arranged by size:
1661 859 234 138 64 48 48 48
48 48 47 45 3
BseYI C'CCAG_C
Cuts at: 0 721 3291
Size: 721 2570
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1029 2948 3291
Size: 593 436 1919 343
Fragments arranged by size:
1919 593 436 343
BsiHKAI G_wGCw'C
Cuts at: 0 390 460 3291
Size: 390 70 2831
Fragments arranged by size:
2831 390 70
Bsp1286I G_dGCh'C
Cuts at: 0 390 460 643 1874 1926 2034 2493 3105
Size: 390 70 183 1231 52 108 459 612
Cuts at: 3105 3291
Size: 186
Fragments arranged by size:
1231 612 459 390 186 183 108 70
52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2130 2154 3291
Size: 2130 24 1137
Fragments arranged by size:
2130 1137 24
BsrBI CCG'CTC
Cuts at: 0 439 3291
Size: 439 2852
BsrFI r'CCGG_y
Cuts at: 0 231 3291
Size: 231 3060
BstAPI GCAn_nnn'nTGC
Cuts at: 0 385 3291
Size: 385 2906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2166 2214 2262 2310 2358 3291
Size: 2166 48 48 48 48 933
Fragments arranged by size:
2166 933 48 48 48 48
BstYI r'GATC_y
Cuts at: 0 414 1810 2574 3291
Size: 414 1396 764 717
Fragments arranged by size:
1396 764 717 414
BstZ17I GTA'TAC
Cuts at: 0 825 3291
Size: 825 2466
BtgI C'CryG_G
Cuts at: 0 1156 1898 2006 2069 2114 2207 2255 2351
Size: 1156 742 108 63 45 93 48 96
Cuts at: 2351 3291
Size: 940
Fragments arranged by size:
1156 940 742 108 96 93 63 48
45
BtsI GCAGTG_nn'
Cuts at: 0 60 611 2596 2957 3291
Size: 60 551 1985 361 334
Fragments arranged by size:
1985 551 361 334 60
ClaI AT'CG_AT
Cuts at: 0 678 3291
Size: 678 2613
DraI TTT'AAA
Cuts at: 0 996 2801 3291
Size: 996 1805 490
Fragments arranged by size:
1805 996 490
DraIII CAC_nnn'GTG
Cuts at: 0 1096 3291
Size: 1096 2195
EaeI y'GGCC_r
Cuts at: 0 2 122 3106 3291
Size: 2 120 2984 185
Fragments arranged by size:
2984 185 120 2
EarI CTCTTCn'nnn_
Cuts at: 0 275 560 612 614 2615 2684 2693 3291
Size: 275 285 52 2 2001 69 9 598
Fragments arranged by size:
2001 598 285 275 69 52 9 2
EcoICRI GAG'CTC
Cuts at: 0 458 3291
Size: 458 2833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 374 455 530 807 2665 2912 3291
Size: 50 324 81 75 277 1858 247 379
Fragments arranged by size:
1858 379 324 277 247 81 75 50
EcoO109I rG'GnC_Cy
Cuts at: 0 639 640 1840 1870 1978 2104 3291
Size: 639 1 1200 30 108 126 1187
Fragments arranged by size:
1200 1187 639 126 108 30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2695 3165 3291
Size: 2695 470 126
Fragments arranged by size:
2695 470 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 797 1597 1629 1795 1812 1827 1844
Size: 765 32 800 32 166 17 15 17
Cuts at: 1844 3052 3084 3291
Size: 1208 32 207
Fragments arranged by size:
1208 800 765 207 166 32 32 32
17 17 15
HincII GTy'rAC
Cuts at: 0 1575 3291
Size: 1575 1716
MfeI C'AATT_G
Cuts at: 0 1167 3291
Size: 1167 2124
MscI TGG'CCA
Cuts at: 0 4 3291
Size: 4 3287
MslI CAynn'nnrTG
Cuts at: 0 67 769 912 980 996 2164 2212 2260
Size: 67 702 143 68 16 1168 48 48
Cuts at: 2260 2308 2356 3038 3291
Size: 48 48 682 253
Fragments arranged by size:
1168 702 682 253 143 68 67 48
48 48 48 16
MspA1I CmG'CkG
Cuts at: 0 182 659 3291
Size: 182 477 2632
Fragments arranged by size:
2632 477 182
NcoI C'CATG_G
Cuts at: 0 1898 2069 2114 2207 2255 2351 3291
Size: 1898 171 45 93 48 96 940
Fragments arranged by size:
1898 940 171 96 93 48 45
NdeI CA'TA_TG
Cuts at: 0 965 3291
Size: 965 2326
NspI r_CATG'y
Cuts at: 0 90 833 883 1138 1150 1909 2478 3069
Size: 90 743 50 255 12 759 569 591
Cuts at: 3069 3291
Size: 222
Fragments arranged by size:
759 743 591 569 255 222 90 50
12
PciI A'CATG_T
Cuts at: 0 86 829 1134 1146 3065 3291
Size: 86 743 305 12 1919 226
Fragments arranged by size:
1919 743 305 226 86 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 3291
Size: 15 3276
PspOMI G'GGCC_C
Cuts at: 0 639 1870 3291
Size: 639 1231 1421
Fragments arranged by size:
1421 1231 639
PstI C_TGCA'G
Cuts at: 0 618 3291
Size: 618 2673
PvuII CAG'CTG
Cuts at: 0 182 3291
Size: 182 3109
SacI G_AGCT'C
Cuts at: 0 460 3291
Size: 460 2831
SapI GCTCTTCn'nnn_
Cuts at: 0 612 3291
Size: 612 2679
SexAI A'CCwGG_T
Cuts at: 0 194 2718 3291
Size: 194 2524 573
Fragments arranged by size:
2524 573 194
SfcI C'TryA_G
Cuts at: 0 614 1210 3291
Size: 614 596 2081
Fragments arranged by size:
2081 614 596
SmlI C'TyrA_G
Cuts at: 0 933 3278 3291
Size: 933 2345 13
Fragments arranged by size:
2345 933 13
SphI G_CATG'C
Cuts at: 0 1909 3291
Size: 1909 1382
SspI AAT'ATT
Cuts at: 0 1320 3291
Size: 1320 1971
StyI C'CwwG_G
Cuts at: 0 635 814 1898 2069 2114 2207 2255 2351
Size: 635 179 1084 171 45 93 48 96
Cuts at: 2351 3149 3291
Size: 798 142
Fragments arranged by size:
1084 798 635 179 171 142 96 93
48 45
SwaI ATTT'AAAT
Cuts at: 0 996 3291
Size: 996 2295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 2017 3291
Size: 22 1995 1274
Fragments arranged by size:
1995 1274 22
TatI w'GTAC_w
Cuts at: 0 1732 2040 3257 3291
Size: 1732 308 1217 34
Fragments arranged by size:
1732 1217 308 34
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 117 718 1588 2964 3291
Size: 12 105 601 870 1376 327
Fragments arranged by size:
1376 870 601 327 105 12
XmnI GAAnn'nnTTC
Cuts at: 0 1524 3291
Size: 1524 1767
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaI
ApoI BaeI BanI BanII BbsI BbvCI BcgI BclI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstXI BstYI BstZ17I BtgI BtsI ClaI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI
Hin4I HincII MfeI MscI MslI MspA1I NcoI NdeI
NspI PciI PflMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SmlI SphI SspI StyI SwaI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BciVI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI
CspCI DrdI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI TstI Tth111I XbaI XhoI XmaI
ZraI