Restriction map for
pF1KB5122
(Linear) (Six-base) MAPSORT of: KIBB5122.seq Check: 7820 from: 1 to: 1542
KIBB5122.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AccI GT'mk_AC
Cuts at: 0 168 225 642 857 1286 1542
Size: 168 57 417 215 429 256
Fragments arranged by size:
429 417 256 215 168 57
Acc65I G'GTAC_C
Cuts at: 0 208 1542
Size: 208 1334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1542
Size: 530 1012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1436 1542
Size: 1436 106
AlwNI CAG_nnn'CTG
Cuts at: 0 328 331 562 1542
Size: 328 3 231 980
Fragments arranged by size:
980 328 231 3
ApoI r'AATT_y
Cuts at: 0 834 1178 1542
Size: 834 344 364
Fragments arranged by size:
834 364 344
AseI AT'TA_AT
Cuts at: 0 197 1542
Size: 197 1345
BanI G'GyrC_C
Cuts at: 0 121 208 1542
Size: 121 87 1334
Fragments arranged by size:
1334 121 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1280 1314 1542
Size: 1280 34 228
Fragments arranged by size:
1280 228 34
BfrBI ATG'CAT
Cuts at: 0 1301 1542
Size: 1301 241
Bme1580I G_kGCm'C
Cuts at: 0 126 1542
Size: 126 1416
BmtI G_CTAG'C
Cuts at: 0 779 1542
Size: 779 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 872 1542
Size: 584 288 670
Fragments arranged by size:
670 584 288
BsaBI GATnn'nnATC
Cuts at: 0 151 1300 1542
Size: 151 1149 242
Fragments arranged by size:
1149 242 151
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1475 1505 1542
Size: 1475 30 37
Fragments arranged by size:
1475 37 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 1542
Size: 415 1127
BsiHKAI G_wGCw'C
Cuts at: 0 414 1425 1542
Size: 414 1011 117
Fragments arranged by size:
1011 414 117
BsmI GAATG_Cn'
Cuts at: 0 989 1479 1542
Size: 989 490 63
Fragments arranged by size:
989 490 63
Bsp1286I G_dGCh'C
Cuts at: 0 126 414 1425 1542
Size: 126 288 1011 117
Fragments arranged by size:
1011 288 126 117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1352 1542
Size: 1352 190
BsrGI T'GTAC_A
Cuts at: 0 1334 1395 1542
Size: 1334 61 147
Fragments arranged by size:
1334 147 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 328 331 1542
Size: 328 3 1211
Fragments arranged by size:
1211 328 3
BstEII G'GTnAC_C
Cuts at: 0 766 1542
Size: 766 776
BstYI r'GATC_y
Cuts at: 0 364 1542
Size: 364 1178
BstZ17I GTA'TAC
Cuts at: 0 643 1542
Size: 643 899
Bsu36I CC'TnA_GG
Cuts at: 0 783 1542
Size: 783 759
BtsI GCAGTG_nn'
Cuts at: 0 18 1542
Size: 18 1524
DraI TTT'AAA
Cuts at: 0 1118 1542
Size: 1118 424
DrdI GACnn_nn'nnGTC
Cuts at: 0 1147 1542
Size: 1147 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 584 872 1542
Size: 530 54 288 670
Fragments arranged by size:
670 530 288 54
EcoO109I rG'GnC_Cy
Cuts at: 0 799 1071 1542
Size: 799 272 471
Fragments arranged by size:
799 471 272
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 730 1397 1542
Size: 730 667 145
Fragments arranged by size:
730 667 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 670 702 1206 1238 1407 1439
Size: 226 32 412 32 504 32 169 32
Cuts at: 1439 1542
Size: 103
Fragments arranged by size:
504 412 226 169 103 32 32 32
32
HincII GTy'rAC
Cuts at: 0 24 226 1542
Size: 24 202 1316
Fragments arranged by size:
1316 202 24
HindIII A'AGCT_T
Cuts at: 0 290 304 1542
Size: 290 14 1238
Fragments arranged by size:
1238 290 14
KpnI G_GTAC'C
Cuts at: 0 212 1542
Size: 212 1330
MfeI C'AATT_G
Cuts at: 0 1143 1542
Size: 1143 399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 1473 1542
Size: 1225 248 69
Fragments arranged by size:
1225 248 69
MslI CAynn'nnrTG
Cuts at: 0 816 890 1046 1542
Size: 816 74 156 496
Fragments arranged by size:
816 496 156 74
MspA1I CmG'CkG
Cuts at: 0 139 328 337 346 1542
Size: 139 189 9 9 1196
Fragments arranged by size:
1196 189 139 9 9
NheI G'CTAG_C
Cuts at: 0 775 1542
Size: 775 767
NsiI A_TGCA'T
Cuts at: 0 1303 1542
Size: 1303 239
PflMI CCAn_nnn'nTGG
Cuts at: 0 752 1542
Size: 752 790
PpuMI rG'GwC_Cy
Cuts at: 0 1071 1542
Size: 1071 471
PstI C_TGCA'G
Cuts at: 0 336 351 1542
Size: 336 15 1191
Fragments arranged by size:
1191 336 15
PvuII CAG'CTG
Cuts at: 0 328 337 346 1542
Size: 328 9 9 1196
Fragments arranged by size:
1196 328 9 9
SalI G'TCGA_C
Cuts at: 0 224 1542
Size: 224 1318
SanDI GG'GwC_CC
Cuts at: 0 1071 1542
Size: 1071 471
ScaI AGT'ACT
Cuts at: 0 629 1542
Size: 629 913
SfcI C'TryA_G
Cuts at: 0 169 332 347 419 1437 1542
Size: 169 163 15 72 1018 105
Fragments arranged by size:
1018 169 163 105 72 15
SspI AAT'ATT
Cuts at: 0 1457 1542
Size: 1457 85
StyI C'CwwG_G
Cuts at: 0 1035 1542
Size: 1035 507
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1304 1542
Size: 1304 238
TatI w'GTAC_w
Cuts at: 0 273 627 976 1334 1395 1542
Size: 273 354 349 358 61 147
Fragments arranged by size:
358 354 349 273 147 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 579 611 1542
Size: 579 32 931
Fragments arranged by size:
931 579 32
XbaI T'CTAG_A
Cuts at: 0 690 1542
Size: 690 852
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApoI AseI BanI
BcgI BfrBI Bme1580I BmtI BpmI BsaBI BsaXI BseRI
BsiHKAI BsmI Bsp1286I BspMI BsrGI BstAPI BstEII BstYI
BstZ17I Bsu36I BtsI DraI DrdI Eco57MI EcoO109I FalI
Hin4I HincII HindIII KpnI MfeI MmeI MslI MspA1I
NheI NsiI PflMI PpuMI PstI PvuII SalI SanDI
ScaI SfcI SspI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstXI BtgI BtgZI ClaI CspCI DraIII EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HpaI KasI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI
XhoI XmaI XmnI ZraI