Restriction map for
pF1KB5138
(Linear) (Six-base) MAPSORT of: KIBB5138.seq Check: 4787 from: 1 to: 2385
KIBB5138.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 2233 2290 2385
Size: 598 1635 57 95
Fragments arranged by size:
1635 598 95 57
AflIII A'CryG_T
Cuts at: 0 1182 2385
Size: 1182 1203
AleI CACnn'nnGTG
Cuts at: 0 562 1108 2385
Size: 562 546 1277
Fragments arranged by size:
1277 562 546
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1735 2385
Size: 1735 650
AlwNI CAG_nnn'CTG
Cuts at: 0 960 978 2127 2385
Size: 960 18 1149 258
Fragments arranged by size:
1149 960 258 18
ApoI r'AATT_y
Cuts at: 0 289 500 703 1290 2045 2385
Size: 289 211 203 587 755 340
Fragments arranged by size:
755 587 340 289 211 203
AseI AT'TA_AT
Cuts at: 0 1481 2366 2385
Size: 1481 885 19
Fragments arranged by size:
1481 885 19
AvaI C'yCGr_G
Cuts at: 0 177 2385
Size: 177 2208
BanI G'GyrC_C
Cuts at: 0 787 1917 2385
Size: 787 1130 468
Fragments arranged by size:
1130 787 468
BanII G_rGCy'C
Cuts at: 0 115 920 1783 2385
Size: 115 805 863 602
Fragments arranged by size:
863 805 602 115
BbeI G_GCGC'C
Cuts at: 0 791 2385
Size: 791 1594
BbsI GAAGACnn'nnnn_
Cuts at: 0 536 739 1106 1663 2032 2385
Size: 536 203 367 557 369 353
Fragments arranged by size:
557 536 369 367 353 203
BbvCI CC'TCA_GC
Cuts at: 0 1676 1871 2385
Size: 1676 195 514
Fragments arranged by size:
1676 514 195
BglII A'GATC_T
Cuts at: 0 1063 2385
Size: 1063 1322
BlpI GC'TnA_GC
Cuts at: 0 971 2385
Size: 971 1414
Bme1580I G_kGCm'C
Cuts at: 0 628 2385
Size: 628 1757
BmgBI CAC'GTC
Cuts at: 0 2091 2385
Size: 2091 294
BmrI ACTGGGnnnn_n'
Cuts at: 0 1251 2385
Size: 1251 1134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 590 1662 1802 2385
Size: 590 1072 140 583
Fragments arranged by size:
1072 590 583 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1601 1979 2056 2385
Size: 118 1483 378 77 329
Fragments arranged by size:
1483 378 329 118 77
Bpu10I CC'TnA_GC
Cuts at: 0 1676 1871 2385
Size: 1676 195 514
Fragments arranged by size:
1676 514 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 2179 2385
Size: 1520 659 206
Fragments arranged by size:
1520 659 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 1313 2385
Size: 1313 1072
BsaAI yAC'GTr
Cuts at: 0 432 1183 2385
Size: 432 751 1202
Fragments arranged by size:
1202 751 432
BsaBI GATnn'nnATC
Cuts at: 0 479 599 2385
Size: 479 120 1786
Fragments arranged by size:
1786 479 120
BsaHI Gr'CG_yC
Cuts at: 0 788 2385
Size: 788 1597
BsaWI w'CCGG_w
Cuts at: 0 887 1305 2224 2385
Size: 887 418 919 161
Fragments arranged by size:
919 887 418 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 129 159 2385
Size: 129 30 2226
Fragments arranged by size:
2226 129 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 1859 2275 2385
Size: 292 1567 416 110
Fragments arranged by size:
1567 416 292 110
BseYI C'CCAG_C
Cuts at: 0 779 1013 1402 2135 2385
Size: 779 234 389 733 250
Fragments arranged by size:
779 733 389 250 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1832 2385
Size: 892 940 553
Fragments arranged by size:
940 892 553
BsmI GAATG_Cn'
Cuts at: 0 576 1362 2385
Size: 576 786 1023
Fragments arranged by size:
1023 786 576
Bsp1286I G_dGCh'C
Cuts at: 0 115 628 920 1783 2385
Size: 115 513 292 863 602
Fragments arranged by size:
863 602 513 292 115
BspEI T'CCGG_A
Cuts at: 0 887 2385
Size: 887 1498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 914 2385
Size: 914 1471
BsrDI GCAATG_nn'
Cuts at: 0 340 2385
Size: 340 2045
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1546 1555 2385
Size: 162 1384 9 830
Fragments arranged by size:
1384 830 162 9
BstYI r'GATC_y
Cuts at: 0 1063 1608 2385
Size: 1063 545 777
Fragments arranged by size:
1063 777 545
Bsu36I CC'TnA_GG
Cuts at: 0 202 2385
Size: 202 2183
BtgI C'CryG_G
Cuts at: 0 867 1370 1728 1852 1972 1982 2202 2385
Size: 867 503 358 124 120 10 220 183
Fragments arranged by size:
867 503 358 220 183 124 120 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1154 2385
Size: 1154 1231
BtsI GCAGTG_nn'
Cuts at: 0 1001 2385
Size: 1001 1384
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1779 1814 2385
Size: 1779 35 571
Fragments arranged by size:
1779 571 35
DraI TTT'AAA
Cuts at: 0 170 2385
Size: 170 2215
EaeI y'GGCC_r
Cuts at: 0 614 807 1367 2032 2385
Size: 614 193 560 665 353
Fragments arranged by size:
665 614 560 353 193
EarI CTCTTCn'nnn_
Cuts at: 0 117 902 1229 2105 2252 2276 2330 2385
Size: 117 785 327 876 147 24 54 55
Fragments arranged by size:
876 785 327 147 117 55 54 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 598 1601 1979 2056 2233 2290 2385
Size: 118 480 1003 378 77 177 57 95
Fragments arranged by size:
1003 480 378 177 118 95 77 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1228 2385
Size: 1228 1157
EcoO109I rG'GnC_Cy
Cuts at: 0 1239 1375 1957 2385
Size: 1239 136 582 428
Fragments arranged by size:
1239 582 428 136
FspI TGC'GCA
Cuts at: 0 857 2385
Size: 857 1528
HaeII r_GCGC'y
Cuts at: 0 791 2385
Size: 791 1594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1145 1177 1944 1976 2385
Size: 1145 32 767 32 409
Fragments arranged by size:
1145 767 409 32 32
HincII GTy'rAC
Cuts at: 0 951 2385
Size: 951 1434
KasI G'GCGC_C
Cuts at: 0 787 2385
Size: 787 1598
MfeI C'AATT_G
Cuts at: 0 943 2385
Size: 943 1442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 2385
Size: 381 2004
MscI TGG'CCA
Cuts at: 0 616 809 2385
Size: 616 193 1576
Fragments arranged by size:
1576 616 193
MslI CAynn'nnrTG
Cuts at: 0 562 1108 1727 2385
Size: 562 546 619 658
Fragments arranged by size:
658 619 562 546
MspA1I CmG'CkG
Cuts at: 0 327 960 975 2184 2385
Size: 327 633 15 1209 201
Fragments arranged by size:
1209 633 327 201 15
NarI GG'CG_CC
Cuts at: 0 788 2385
Size: 788 1597
NcoI C'CATG_G
Cuts at: 0 867 1370 1728 1852 2385
Size: 867 503 358 124 533
Fragments arranged by size:
867 533 503 358 124
NspI r_CATG'y
Cuts at: 0 286 2385
Size: 286 2099
PasI CC'CwG_GG
Cuts at: 0 913 2385
Size: 913 1472
PflMI CCAn_nnn'nTGG
Cuts at: 0 960 2385
Size: 960 1425
PmlI CAC'GTG
Cuts at: 0 1183 2385
Size: 1183 1202
PpuMI rG'GwC_Cy
Cuts at: 0 1239 1375 2385
Size: 1239 136 1010
Fragments arranged by size:
1239 1010 136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 652 684 2170 2202 2385
Size: 652 32 1486 32 183
Fragments arranged by size:
1486 652 183 32 32
PvuII CAG'CTG
Cuts at: 0 327 960 975 2385
Size: 327 633 15 1410
Fragments arranged by size:
1410 633 327 15
SapI GCTCTTCn'nnn_
Cuts at: 0 2330 2385
Size: 2330 55
ScaI AGT'ACT
Cuts at: 0 666 2385
Size: 666 1719
SfcI C'TryA_G
Cuts at: 0 452 2385
Size: 452 1933
SfoI GGC'GCC
Cuts at: 0 789 2385
Size: 789 1596
SmlI C'TyrA_G
Cuts at: 0 1535 2194 2385
Size: 1535 659 191
Fragments arranged by size:
1535 659 191
SphI G_CATG'C
Cuts at: 0 286 2385
Size: 286 2099
StuI AGG'CCT
Cuts at: 0 1835 2385
Size: 1835 550
StyI C'CwwG_G
Cuts at: 0 617 867 1370 1728 1797 1852 2060 2385
Size: 617 250 503 358 69 55 208 325
Fragments arranged by size:
617 503 358 325 250 208 69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1337 1416 2385
Size: 1337 79 969
Fragments arranged by size:
1337 969 79
TatI w'GTAC_w
Cuts at: 0 139 664 2153 2385
Size: 139 525 1489 232
Fragments arranged by size:
1489 525 232 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 2385
Size: 129 32 2224
Fragments arranged by size:
2224 129 32
Tth111I GACn'n_nGTC
Cuts at: 0 2127 2385
Size: 2127 258
XbaI T'CTAG_A
Cuts at: 0 1813 2385
Size: 1813 572
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BbvCI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI
Bsp1286I BspEI BspMI BsrDI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI EaeI EarI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NspI PasI PflMI
PmlI PpuMI PsrI PvuII SapI ScaI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglI BmtI BsiEI
BsiHKAI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I ClaI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XcmI XhoI
XmaI XmnI ZraI