Restriction map for
pF1KB5139
(Linear) (Six-base) MAPSORT of: KIBB5139.seq Check: 868 from: 1 to: 1701
KIBB5139.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1219 1701
Size: 1219 482
AccI GT'mk_AC
Cuts at: 0 695 1701
Size: 695 1006
Acc65I G'GTAC_C
Cuts at: 0 1038 1701
Size: 1038 663
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1470 1554 1701
Size: 1470 84 147
Fragments arranged by size:
1470 147 84
AfeI AGC'GCT
Cuts at: 0 730 814 1162 1701
Size: 730 84 348 539
Fragments arranged by size:
730 539 348 84
AleI CACnn'nnGTG
Cuts at: 0 130 359 1701
Size: 130 229 1342
Fragments arranged by size:
1342 229 130
AlwNI CAG_nnn'CTG
Cuts at: 0 90 1701
Size: 90 1611
ApaI G_GGCC'C
Cuts at: 0 25 68 168 411 641 1077 1176 1701
Size: 25 43 100 243 230 436 99 525
Fragments arranged by size:
525 436 243 230 100 99 43 25
ApaLI G'TGCA_C
Cuts at: 0 592 769 1270 1701
Size: 592 177 501 431
Fragments arranged by size:
592 501 431 177
AvaI C'yCGr_G
Cuts at: 0 799 978 1701
Size: 799 179 723
Fragments arranged by size:
799 723 179
BanI G'GyrC_C
Cuts at: 0 145 233 497 1038 1621 1701
Size: 145 88 264 541 583 80
Fragments arranged by size:
583 541 264 145 88 80
BanII G_rGCy'C
Cuts at: 0 25 68 168 411 486 641 988 1028
Size: 25 43 100 243 75 155 347 40
Cuts at: 1028 1077 1176 1701
Size: 49 99 525
Fragments arranged by size:
525 347 243 155 100 99 75 49
43 40 25
BbeI G_GCGC'C
Cuts at: 0 1625 1701
Size: 1625 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 1249 1701
Size: 1249 452
BbvCI CC'TCA_GC
Cuts at: 0 112 602 1701
Size: 112 490 1099
Fragments arranged by size:
1099 490 112
BglI GCCn_nnn'nGGC
Cuts at: 0 517 1701
Size: 517 1184
Bme1580I G_kGCm'C
Cuts at: 0 25 68 150 168 180 411 596 641
Size: 25 43 82 18 12 231 185 45
Cuts at: 641 773 799 1077 1176 1274 1701
Size: 132 26 278 99 98 427
Fragments arranged by size:
427 278 231 185 132 99 98 82
45 43 26 25 18 12
BmgBI CAC'GTC
Cuts at: 0 128 1701
Size: 128 1573
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 555 1701
Size: 132 423 1146
Fragments arranged by size:
1146 423 132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1177 1701
Size: 631 546 524
Fragments arranged by size:
631 546 524
Bpu10I CC'TnA_GC
Cuts at: 0 112 602 1701
Size: 112 490 1099
Fragments arranged by size:
1099 490 112
BsaAI yAC'GTr
Cuts at: 0 597 1701
Size: 597 1104
BsaHI Gr'CG_yC
Cuts at: 0 1622 1701
Size: 1622 79
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 677 707 1701
Size: 677 30 994
Fragments arranged by size:
994 677 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 628 1315 1678 1701
Size: 628 687 363 23
Fragments arranged by size:
687 628 363 23
BseYI C'CCAG_C
Cuts at: 0 44 203 380 989 1701
Size: 44 159 177 609 712
Fragments arranged by size:
712 609 177 159 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1701
Size: 289 1412
BsiEI CG_ry'CG
Cuts at: 0 699 894 1022 1103 1311 1368 1563 1701
Size: 699 195 128 81 208 57 195 138
Fragments arranged by size:
699 208 195 195 138 128 81 57
BsiHKAI G_wGCw'C
Cuts at: 0 486 596 773 1088 1274 1701
Size: 486 110 177 315 186 427
Fragments arranged by size:
486 427 315 186 177 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 447 1701
Size: 447 1254
Bsp1286I G_dGCh'C
Cuts at: 0 25 68 150 168 180 411 486 596
Size: 25 43 82 18 12 231 75 110
Cuts at: 596 641 773 799 988 1028 1077 1088 1176
Size: 45 132 26 189 40 49 11 88
Cuts at: 1176 1274 1701
Size: 98 427
Fragments arranged by size:
427 231 189 132 110 98 88 82
75 49 45 43 40 26 25 18
12 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 926 1219 1701
Size: 926 293 482
Fragments arranged by size:
926 482 293
BsrBI CCG'CTC
Cuts at: 0 783 1200 1284 1536 1701
Size: 783 417 84 252 165
Fragments arranged by size:
783 417 252 165 84
BsrFI r'CCGG_y
Cuts at: 0 230 845 859 1000 1050 1430 1444 1514
Size: 230 615 14 141 50 380 14 70
Cuts at: 1514 1701
Size: 187
Fragments arranged by size:
615 380 230 187 141 70 50 14
14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 887 1701
Size: 887 814
BstEII G'GTnAC_C
Cuts at: 0 663 1701
Size: 663 1038
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 361 633 1701
Size: 39 322 272 1068
Fragments arranged by size:
1068 322 272 39
BtgI C'CryG_G
Cuts at: 0 32 105 659 1016 1701
Size: 32 73 554 357 685
Fragments arranged by size:
685 554 357 73 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 154 417 1701
Size: 154 263 1284
Fragments arranged by size:
1284 263 154
BtsI GCAGTG_nn'
Cuts at: 0 301 1701
Size: 301 1400
DraIII CAC_nnn'GTG
Cuts at: 0 1254 1701
Size: 1254 447
EaeI y'GGCC_r
Cuts at: 0 29 270 546 891 1019 1308 1644 1701
Size: 29 241 276 345 128 289 336 57
Fragments arranged by size:
345 336 289 276 241 128 57 29
EagI C'GGCC_G
Cuts at: 0 891 1019 1308 1701
Size: 891 128 289 393
Fragments arranged by size:
891 393 289 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1393 1701
Size: 1393 308
EcoICRI GAG'CTC
Cuts at: 0 484 1701
Size: 484 1217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1177 1470 1554 1701
Size: 631 546 293 84 147
Fragments arranged by size:
631 546 293 147 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 613 1701
Size: 94 519 1088
Fragments arranged by size:
1088 519 94
EcoO109I rG'GnC_Cy
Cuts at: 0 21 22 65 164 606 637 638 1073
Size: 21 1 43 99 442 31 1 435
Cuts at: 1073 1074 1095 1701
Size: 1 21 606
Fragments arranged by size:
606 442 435 99 43 31 21 21
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 765 1701
Size: 765 936
HaeII r_GCGC'y
Cuts at: 0 732 816 1164 1625 1701
Size: 732 84 348 461 76
Fragments arranged by size:
732 461 348 84 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 677 709 1701
Size: 677 32 992
Fragments arranged by size:
992 677 32
HincII GTy'rAC
Cuts at: 0 696 1701
Size: 696 1005
KasI G'GCGC_C
Cuts at: 0 1621 1701
Size: 1621 80
KpnI G_GTAC'C
Cuts at: 0 1042 1701
Size: 1042 659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1523 1607 1701
Size: 1523 84 94
Fragments arranged by size:
1523 94 84
MscI TGG'CCA
Cuts at: 0 31 1646 1701
Size: 31 1615 55
Fragments arranged by size:
1615 55 31
MslI CAynn'nnrTG
Cuts at: 0 130 173 359 1701
Size: 130 43 186 1342
Fragments arranged by size:
1342 186 130 43
MspA1I CmG'CkG
Cuts at: 0 107 418 661 993 1018 1127 1182 1701
Size: 107 311 243 332 25 109 55 519
Fragments arranged by size:
519 332 311 243 109 107 55 25
NaeI GCC'GGC
Cuts at: 0 1002 1701
Size: 1002 699
NarI GG'CG_CC
Cuts at: 0 1622 1701
Size: 1622 79
NcoI C'CATG_G
Cuts at: 0 32 1701
Size: 32 1669
NdeI CA'TA_TG
Cuts at: 0 532 1701
Size: 532 1169
NgoMIV G'CCGG_C
Cuts at: 0 1000 1701
Size: 1000 701
NotI GC'GGCC_GC
Cuts at: 0 891 1308 1701
Size: 891 417 393
Fragments arranged by size:
891 417 393
PasI CC'CwG_GG
Cuts at: 0 90 647 1323 1701
Size: 90 557 676 378
Fragments arranged by size:
676 557 378 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 90 1701
Size: 90 1611
PfoI T'CCnGG_A
Cuts at: 0 1613 1701
Size: 1613 88
PpuMI rG'GwC_Cy
Cuts at: 0 1095 1701
Size: 1095 606
PspOMI G'GGCC_C
Cuts at: 0 21 64 164 407 637 1073 1172 1701
Size: 21 43 100 243 230 436 99 529
Fragments arranged by size:
529 436 243 230 100 99 43 21
PvuI CG_AT'CG
Cuts at: 0 1103 1368 1701
Size: 1103 265 333
Fragments arranged by size:
1103 333 265
PvuII CAG'CTG
Cuts at: 0 418 993 1127 1701
Size: 418 575 134 574
Fragments arranged by size:
575 574 418 134
SacI G_AGCT'C
Cuts at: 0 486 1701
Size: 486 1215
SacII CC_GC'GG
Cuts at: 0 108 662 1019 1701
Size: 108 554 357 682
Fragments arranged by size:
682 554 357 108
SalI G'TCGA_C
Cuts at: 0 694 1701
Size: 694 1007
SexAI A'CCwGG_T
Cuts at: 0 1250 1502 1701
Size: 1250 252 199
Fragments arranged by size:
1250 252 199
SfcI C'TryA_G
Cuts at: 0 1122 1701
Size: 1122 579
SfoI GGC'GCC
Cuts at: 0 1623 1701
Size: 1623 78
SgrAI Cr'CCGG_yG
Cuts at: 0 230 845 859 1430 1444 1514 1701
Size: 230 615 14 571 14 70 187
Fragments arranged by size:
615 571 230 187 70 14 14
SmaI CCC'GGG
Cuts at: 0 980 1701
Size: 980 721
StuI AGG'CCT
Cuts at: 0 1399 1701
Size: 1399 302
StyI C'CwwG_G
Cuts at: 0 32 626 1701
Size: 32 594 1075
Fragments arranged by size:
1075 594 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 372 1103 1609 1701
Size: 195 177 731 506 92
Fragments arranged by size:
731 506 195 177 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 931 963 1701
Size: 931 32 738
Fragments arranged by size:
931 738 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 1701
Size: 336 1365
XmaI C'CCGG_G
Cuts at: 0 978 1701
Size: 978 723
XmnI GAAnn'nnTTC
Cuts at: 0 1231 1701
Size: 1231 470
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BstAPI BstEII BstXI BtgI BtgZI BtsI
DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI
PasI PflMI PfoI PpuMI PspOMI PvuI PvuII SacI
SacII SalI SexAI SfcI SfoI SgrAI SmaI StuI
StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EarI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI NheI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI RsrII SanDI SapI SbfI ScaI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI ZraI