Restriction map for
pF1KB5141
(Linear) (Six-base) MAPSORT of: KIBB5141.seq Check: 6437 from: 1 to: 1569
KIBB5141.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 156 321 1569
Size: 156 165 1248
Fragments arranged by size:
1248 165 156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1461 1569
Size: 604 857 108
Fragments arranged by size:
857 604 108
AflIII A'CryG_T
Cuts at: 0 914 1388 1569
Size: 914 474 181
Fragments arranged by size:
914 474 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1318 1569
Size: 1318 251
AlwNI CAG_nnn'CTG
Cuts at: 0 56 1138 1569
Size: 56 1082 431
Fragments arranged by size:
1082 431 56
ApaI G_GGCC'C
Cuts at: 0 974 1569
Size: 974 595
ApoI r'AATT_y
Cuts at: 0 1286 1569
Size: 1286 283
AvaI C'yCGr_G
Cuts at: 0 115 229 973 1569
Size: 115 114 744 596
Fragments arranged by size:
744 596 115 114
BamHI G'GATC_C
Cuts at: 0 410 1569
Size: 410 1159
BanII G_rGCy'C
Cuts at: 0 230 677 743 974 998 1569
Size: 230 447 66 231 24 571
Fragments arranged by size:
571 447 231 230 66 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 1268 1569
Size: 1268 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1413 1447 1569
Size: 1413 34 122
Fragments arranged by size:
1413 122 34
BclI T'GATC_A
Cuts at: 0 1067 1569
Size: 1067 502
BglI GCCn_nnn'nGGC
Cuts at: 0 88 1569
Size: 88 1481
Bme1580I G_kGCm'C
Cuts at: 0 405 974 1129 1439 1569
Size: 405 569 155 310 130
Fragments arranged by size:
569 405 310 155 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 1126 1569
Size: 1126 443
BmtI G_CTAG'C
Cuts at: 0 458 1569
Size: 458 1111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1017 1569
Size: 1017 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 908 966 1367 1432 1481 1569
Size: 169 739 58 401 65 49 88
Fragments arranged by size:
739 401 169 88 65 58 49
Bpu10I CC'TnA_GC
Cuts at: 0 1364 1569
Size: 1364 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 193 1569
Size: 88 105 1376
Fragments arranged by size:
1376 105 88
BsaBI GATnn'nnATC
Cuts at: 0 634 1486 1569
Size: 634 852 83
Fragments arranged by size:
852 634 83
BsaHI Gr'CG_yC
Cuts at: 0 290 680 1569
Size: 290 390 889
Fragments arranged by size:
889 390 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1000 1030 1569
Size: 1000 30 539
Fragments arranged by size:
1000 539 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 724 1064 1201 1429 1569
Size: 724 340 137 228 140
Fragments arranged by size:
724 340 228 140 137
BseYI C'CCAG_C
Cuts at: 0 49 373 719 1569
Size: 49 324 346 850
Fragments arranged by size:
850 346 324 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1060 1569
Size: 775 285 509
Fragments arranged by size:
775 509 285
BsiEI CG_ry'CG
Cuts at: 0 83 1569
Size: 83 1486
BsiHKAI G_wGCw'C
Cuts at: 0 104 209 230 677 743 791 998 1569
Size: 104 105 21 447 66 48 207 571
Fragments arranged by size:
571 447 207 105 104 66 48 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 600 1569
Size: 600 969
Bsp1286I G_dGCh'C
Cuts at: 0 104 209 230 405 677 743 791 974
Size: 104 105 21 175 272 66 48 183
Cuts at: 974 998 1129 1439 1569
Size: 24 131 310 130
Fragments arranged by size:
310 272 183 175 131 130 105 104
66 48 24 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 321 368 1082 1569
Size: 156 165 47 714 487
Fragments arranged by size:
714 487 165 156 47
BsrFI r'CCGG_y
Cuts at: 0 125 214 1569
Size: 125 89 1355
Fragments arranged by size:
1355 125 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 839 1569
Size: 839 730
BstXI CCAn_nnnn'nTGG
Cuts at: 0 990 1353 1569
Size: 990 363 216
Fragments arranged by size:
990 363 216
BstYI r'GATC_y
Cuts at: 0 410 587 1331 1569
Size: 410 177 744 238
Fragments arranged by size:
744 410 238 177
Bsu36I CC'TnA_GG
Cuts at: 0 782 1569
Size: 782 787
BtgI C'CryG_G
Cuts at: 0 551 1151 1569
Size: 551 600 418
Fragments arranged by size:
600 551 418
BtsI GCAGTG_nn'
Cuts at: 0 1290 1449 1569
Size: 1290 159 120
Fragments arranged by size:
1290 159 120
EaeI y'GGCC_r
Cuts at: 0 80 1238 1569
Size: 80 1158 331
Fragments arranged by size:
1158 331 80
EagI C'GGCC_G
Cuts at: 0 80 1569
Size: 80 1489
EarI CTCTTCn'nnn_
Cuts at: 0 866 1569
Size: 866 703
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 423 1569
Size: 423 1146
EcoICRI GAG'CTC
Cuts at: 0 228 675 741 996 1569
Size: 228 447 66 255 573
Fragments arranged by size:
573 447 255 228 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 604 908 966 1367 1432 1461 1481
Size: 169 435 304 58 401 65 29 20
Cuts at: 1481 1569
Size: 88
Fragments arranged by size:
435 401 304 169 88 65 58 29
20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 706 1569
Size: 328 378 863
Fragments arranged by size:
863 378 328
EcoO109I rG'GnC_Cy
Cuts at: 0 196 443 970 1569
Size: 196 247 527 599
Fragments arranged by size:
599 527 247 196
EcoRI G'AATT_C
Cuts at: 0 1286 1569
Size: 1286 283
HaeII r_GCGC'y
Cuts at: 0 19 727 1569
Size: 19 708 842
Fragments arranged by size:
842 708 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 1204 1236 1569
Size: 266 32 906 32 333
Fragments arranged by size:
906 333 266 32 32
HincII GTy'rAC
Cuts at: 0 468 1569
Size: 468 1101
MscI TGG'CCA
Cuts at: 0 1240 1569
Size: 1240 329
MslI CAynn'nnrTG
Cuts at: 0 688 1063 1162 1569
Size: 688 375 99 407
Fragments arranged by size:
688 407 375 99
MspA1I CmG'CkG
Cuts at: 0 6 240 661 1098 1569
Size: 6 234 421 437 471
Fragments arranged by size:
471 437 421 234 6
NaeI GCC'GGC
Cuts at: 0 127 216 1569
Size: 127 89 1353
Fragments arranged by size:
1353 127 89
NcoI C'CATG_G
Cuts at: 0 1151 1569
Size: 1151 418
NgoMIV G'CCGG_C
Cuts at: 0 125 214 1569
Size: 125 89 1355
Fragments arranged by size:
1355 125 89
NheI G'CTAG_C
Cuts at: 0 454 1569
Size: 454 1115
NotI GC'GGCC_GC
Cuts at: 0 80 1569
Size: 80 1489
NspI r_CATG'y
Cuts at: 0 1392 1569
Size: 1392 177
PasI CC'CwG_GG
Cuts at: 0 805 1569
Size: 805 764
PciI A'CATG_T
Cuts at: 0 1388 1569
Size: 1388 181
PflMI CCAn_nnn'nTGG
Cuts at: 0 56 300 1138 1377 1569
Size: 56 244 838 239 192
Fragments arranged by size:
838 244 239 192 56
PfoI T'CCnGG_A
Cuts at: 0 1409 1553 1569
Size: 1409 144 16
Fragments arranged by size:
1409 144 16
PpuMI rG'GwC_Cy
Cuts at: 0 196 443 1569
Size: 196 247 1126
Fragments arranged by size:
1126 247 196
PspOMI G'GGCC_C
Cuts at: 0 970 1569
Size: 970 599
PspXI vC'TCGA_Gb
Cuts at: 0 229 1569
Size: 229 1340
PstI C_TGCA'G
Cuts at: 0 239 419 1526 1569
Size: 239 180 1107 43
Fragments arranged by size:
1107 239 180 43
PvuII CAG'CTG
Cuts at: 0 661 1098 1569
Size: 661 437 471
Fragments arranged by size:
661 471 437
SacI G_AGCT'C
Cuts at: 0 230 677 743 998 1569
Size: 230 447 66 255 571
Fragments arranged by size:
571 447 255 230 66
SanDI GG'GwC_CC
Cuts at: 0 196 1569
Size: 196 1373
SbfI CC_TGCA'GG
Cuts at: 0 419 1569
Size: 419 1150
ScaI AGT'ACT
Cuts at: 0 979 1569
Size: 979 590
SfcI C'TryA_G
Cuts at: 0 235 415 447 1522 1569
Size: 235 180 32 1075 47
Fragments arranged by size:
1075 235 180 47 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 88 1569
Size: 88 1481
SmlI C'TyrA_G
Cuts at: 0 103 208 229 1569
Size: 103 105 21 1340
Fragments arranged by size:
1340 105 103 21
StuI AGG'CCT
Cuts at: 0 91 1569
Size: 91 1478
StyI C'CwwG_G
Cuts at: 0 1019 1151 1569
Size: 1019 132 418
Fragments arranged by size:
1019 418 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1212 1569
Size: 1212 357
TatI w'GTAC_w
Cuts at: 0 917 977 1569
Size: 917 60 592
Fragments arranged by size:
917 592 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 188 220 1000 1032 1569
Size: 188 32 780 32 537
Fragments arranged by size:
780 537 188 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1159 1569
Size: 1159 410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1026 1569
Size: 1026 543
XhoI C'TCGA_G
Cuts at: 0 229 1569
Size: 229 1340
XmnI GAAnn'nnTTC
Cuts at: 0 1537 1569
Size: 1537 32
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApoI AvaI
BamHI BanII BbsI BcgI BclI BglI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacI SanDI SbfI
ScaI SfcI SfiI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BciVI BfrBI BglII BlpI BmgBI
BsaI BsaAI BsaWI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SapI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI ZraI