Restriction map for
pF1KB5152
(Linear) (Six-base) MAPSORT of: KIBB5152.seq Check: 4342 from: 1 to: 1962
KIBB5152.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1453 1962
Size: 1453 509
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 207 1582 1962
Size: 48 159 1375 380
Fragments arranged by size:
1375 380 159 48
AflIII A'CryG_T
Cuts at: 0 1520 1962
Size: 1520 442
AlwNI CAG_nnn'CTG
Cuts at: 0 1086 1531 1962
Size: 1086 445 431
Fragments arranged by size:
1086 445 431
ApaI G_GGCC'C
Cuts at: 0 410 713 1578 1962
Size: 410 303 865 384
Fragments arranged by size:
865 410 384 303
AvaI C'yCGr_G
Cuts at: 0 403 690 959 1109 1342 1571 1667 1962
Size: 403 287 269 150 233 229 96 295
Fragments arranged by size:
403 295 287 269 233 229 150 96
BanI G'GyrC_C
Cuts at: 0 21 232 316 1126 1408 1465 1962
Size: 21 211 84 810 282 57 497
Fragments arranged by size:
810 497 282 211 84 57 21
BanII G_rGCy'C
Cuts at: 0 47 410 680 713 730 860 958 1075
Size: 47 363 270 33 17 130 98 117
Cuts at: 1075 1122 1578 1962
Size: 47 456 384
Fragments arranged by size:
456 384 363 270 130 117 98 47
47 33 17
BbeI G_GCGC'C
Cuts at: 0 25 320 1962
Size: 25 295 1642
Fragments arranged by size:
1642 295 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 529 1187 1962
Size: 529 658 775
Fragments arranged by size:
775 658 529
BbvCI CC'TCA_GC
Cuts at: 0 669 882 1962
Size: 669 213 1080
Fragments arranged by size:
1080 669 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 992 1026 1962
Size: 992 34 936
Fragments arranged by size:
992 936 34
BglI GCCn_nnn'nGGC
Cuts at: 0 118 701 767 953 1358 1406 1474 1573
Size: 118 583 66 186 405 48 68 99
Cuts at: 1573 1962
Size: 389
Fragments arranged by size:
583 405 389 186 118 99 68 66
48
Bme1580I G_kGCm'C
Cuts at: 0 34 235 410 713 1231 1578 1958 1962
Size: 34 201 175 303 518 347 380 4
Fragments arranged by size:
518 380 347 303 201 175 34 4
BmgBI CAC'GTC
Cuts at: 0 1523 1962
Size: 1523 439
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 1962
Size: 435 1527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 897 1962
Size: 702 195 1065
Fragments arranged by size:
1065 702 195
Bpu10I CC'TnA_GC
Cuts at: 0 165 564 669 882 1930 1962
Size: 165 399 105 213 1048 32
Fragments arranged by size:
1048 399 213 165 105 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1017 1962
Size: 169 848 945
Fragments arranged by size:
945 848 169
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 1886 1962
Size: 1159 727 76
Fragments arranged by size:
1159 727 76
BsaAI yAC'GTr
Cuts at: 0 645 899 1206 1962
Size: 645 254 307 756
Fragments arranged by size:
756 645 307 254
BsaHI Gr'CG_yC
Cuts at: 0 22 317 908 1962
Size: 22 295 591 1054
Fragments arranged by size:
1054 591 295 22
BsaWI w'CCGG_w
Cuts at: 0 518 1962
Size: 518 1444
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 830 860 1507 1537 1962
Size: 436 30 364 30 647 30 425
Fragments arranged by size:
647 436 425 364 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 189 870 1513 1528 1558 1561 1962
Size: 189 681 643 15 30 3 401
Fragments arranged by size:
681 643 401 189 30 15 3
BseYI C'CCAG_C
Cuts at: 0 160 211 559 616 717 1138 1302 1396
Size: 160 51 348 57 101 421 164 94
Cuts at: 1396 1713 1962
Size: 317 249
Fragments arranged by size:
421 348 317 249 164 160 101 94
57 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 787 1717 1759 1962
Size: 646 141 930 42 203
Fragments arranged by size:
930 646 203 141 42
BsiEI CG_ry'CG
Cuts at: 0 1478 1962
Size: 1478 484
BsiHKAI G_wGCw'C
Cuts at: 0 47 680 1211 1962
Size: 47 633 531 751
Fragments arranged by size:
751 633 531 47
BsiWI C'GTAC_G
Cuts at: 0 899 1962
Size: 899 1063
BsmBI CGTCTCn'nnnn_
Cuts at: 0 332 915 1962
Size: 332 583 1047
Fragments arranged by size:
1047 583 332
Bsp1286I G_dGCh'C
Cuts at: 0 34 47 235 410 680 713 730 860
Size: 34 13 188 175 270 33 17 130
Cuts at: 860 958 1075 1122 1211 1231 1578 1958 1962
Size: 98 117 47 89 20 347 380 4
Fragments arranged by size:
380 347 270 188 175 130 117 98
89 47 34 33 20 17 13 4
BspEI T'CCGG_A
Cuts at: 0 518 1962
Size: 518 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1453 1962
Size: 1453 509
BsrBI CCG'CTC
Cuts at: 0 305 312 481 1635 1674 1824 1962
Size: 305 7 169 1154 39 150 138
Fragments arranged by size:
1154 305 169 150 138 39 7
BsrFI r'CCGG_y
Cuts at: 0 104 155 1234 1288 1962
Size: 104 51 1079 54 674
Fragments arranged by size:
1079 674 104 54 51
BssSI C'ACGA_G
Cuts at: 0 811 1962
Size: 811 1151
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1251 1815 1962
Size: 1251 564 147
Fragments arranged by size:
1251 564 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 835 1302 1962
Size: 279 556 467 660
Fragments arranged by size:
660 556 467 279
Bsu36I CC'TnA_GG
Cuts at: 0 1278 1962
Size: 1278 684
BtgI C'CryG_G
Cuts at: 0 1067 1158 1223 1263 1469 1962
Size: 1067 91 65 40 206 493
Fragments arranged by size:
1067 493 206 91 65 40
BtsI GCAGTG_nn'
Cuts at: 0 780 1962
Size: 780 1182
DraIII CAC_nnn'GTG
Cuts at: 0 1224 1962
Size: 1224 738
EaeI y'GGCC_r
Cuts at: 0 119 1260 1475 1655 1962
Size: 119 1141 215 180 307
Fragments arranged by size:
1141 307 215 180 119
EagI C'GGCC_G
Cuts at: 0 1475 1962
Size: 1475 487
EarI CTCTTCn'nnn_
Cuts at: 0 270 1859 1962
Size: 270 1589 103
Fragments arranged by size:
1589 270 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 489 594 1962
Size: 489 105 1368
Fragments arranged by size:
1368 489 105
EcoICRI GAG'CTC
Cuts at: 0 45 678 1962
Size: 45 633 1284
Fragments arranged by size:
1284 633 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 207 702 897 1582 1962
Size: 48 159 495 195 685 380
Fragments arranged by size:
685 495 380 195 159 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 1112 1192 1777 1962
Size: 361 751 80 585 185
Fragments arranged by size:
751 585 361 185 80
EcoO109I rG'GnC_Cy
Cuts at: 0 407 434 525 1083 1566 1962
Size: 407 27 91 558 483 396
Fragments arranged by size:
558 483 407 396 91 27
FseI GG_CCGG'CC
Cuts at: 0 108 1962
Size: 108 1854
HaeII r_GCGC'y
Cuts at: 0 25 320 1273 1650 1962
Size: 25 295 953 377 312
Fragments arranged by size:
953 377 312 295 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 275 307 1507 1539 1962
Size: 275 32 1200 32 423
Fragments arranged by size:
1200 423 275 32 32
KasI G'GCGC_C
Cuts at: 0 21 316 1962
Size: 21 295 1646
Fragments arranged by size:
1646 295 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1308 1869 1962
Size: 1308 561 93
Fragments arranged by size:
1308 561 93
MscI TGG'CCA
Cuts at: 0 1262 1657 1962
Size: 1262 395 305
Fragments arranged by size:
1262 395 305
MslI CAynn'nnrTG
Cuts at: 0 1204 1962
Size: 1204 758
MspA1I CmG'CkG
Cuts at: 0 81 182 738 831 935 973 1528 1962
Size: 81 101 556 93 104 38 555 434
Fragments arranged by size:
556 555 434 104 101 93 81 38
NaeI GCC'GGC
Cuts at: 0 106 1236 1962
Size: 106 1130 726
Fragments arranged by size:
1130 726 106
NarI GG'CG_CC
Cuts at: 0 22 317 1962
Size: 22 295 1645
Fragments arranged by size:
1645 295 22
NgoMIV G'CCGG_C
Cuts at: 0 104 1234 1962
Size: 104 1130 728
Fragments arranged by size:
1130 728 104
NotI GC'GGCC_GC
Cuts at: 0 1475 1962
Size: 1475 487
NspI r_CATG'y
Cuts at: 0 1245 1725 1962
Size: 1245 480 237
Fragments arranged by size:
1245 480 237
PasI CC'CwG_GG
Cuts at: 0 1080 1962
Size: 1080 882
PflMI CCAn_nnn'nTGG
Cuts at: 0 1086 1224 1962
Size: 1086 138 738
Fragments arranged by size:
1086 738 138
PfoI T'CCnGG_A
Cuts at: 0 56 1962
Size: 56 1906
PmlI CAC'GTG
Cuts at: 0 1206 1962
Size: 1206 756
PpuMI rG'GwC_Cy
Cuts at: 0 434 525 1962
Size: 434 91 1437
Fragments arranged by size:
1437 434 91
PshAI GACnn'nnGTC
Cuts at: 0 1151 1962
Size: 1151 811
PspOMI G'GGCC_C
Cuts at: 0 406 709 1574 1962
Size: 406 303 865 388
Fragments arranged by size:
865 406 388 303
PspXI vC'TCGA_Gb
Cuts at: 0 1109 1962
Size: 1109 853
PstI C_TGCA'G
Cuts at: 0 1169 1962
Size: 1169 793
PvuII CAG'CTG
Cuts at: 0 831 973 1962
Size: 831 142 989
Fragments arranged by size:
989 831 142
SacI G_AGCT'C
Cuts at: 0 47 680 1962
Size: 47 633 1282
Fragments arranged by size:
1282 633 47
ScaI AGT'ACT
Cuts at: 0 445 1962
Size: 445 1517
SfcI C'TryA_G
Cuts at: 0 1165 1443 1962
Size: 1165 278 519
Fragments arranged by size:
1165 519 278
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1573 1962
Size: 1573 389
SfoI GGC'GCC
Cuts at: 0 23 318 1962
Size: 23 295 1644
Fragments arranged by size:
1644 295 23
SmaI CCC'GGG
Cuts at: 0 405 692 1573 1669 1962
Size: 405 287 881 96 293
Fragments arranged by size:
881 405 293 287 96
SmlI C'TyrA_G
Cuts at: 0 148 1032 1109 1962
Size: 148 884 77 853
Fragments arranged by size:
884 853 148 77
StuI AGG'CCT
Cuts at: 0 1322 1702 1928 1962
Size: 1322 380 226 34
Fragments arranged by size:
1322 380 226 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 573 709 1294 1779 1937 1962
Size: 250 323 136 585 485 158 25
Fragments arranged by size:
585 485 323 250 158 136 25
TatI w'GTAC_w
Cuts at: 0 443 1962
Size: 443 1519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 1962
Size: 830 32 1100
Fragments arranged by size:
1100 830 32
XhoI C'TCGA_G
Cuts at: 0 1109 1962
Size: 1109 853
XmaI C'CCGG_G
Cuts at: 0 403 690 1571 1667 1962
Size: 403 287 881 96 295
Fragments arranged by size:
881 403 295 287 96
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstAPI BstXI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI ScaI SfcI SfiI SfoI SmaI
SmlI StuI TaqII TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI
BmtI BplI BsaBI BsmI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NruI
NsiI PacI PciI PmeI PpiI PsiI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI
Tth111I XbaI XcmI XmnI ZraI