Restriction map for
pF1KB5159
(Linear) (Six-base) MAPSORT of: KIBB5159.seq Check: 9806 from: 1 to: 588
KIBB5159.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 588
Size: 144 444
AatII G_ACGT'C
Cuts at: 0 236 588
Size: 236 352
AfeI AGC'GCT
Cuts at: 0 544 588
Size: 544 44
AvaI C'yCGr_G
Cuts at: 0 91 286 301 469 588
Size: 91 195 15 168 119
Fragments arranged by size:
195 168 119 91 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 501 588
Size: 105 396 87
Fragments arranged by size:
396 105 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 460 494 588
Size: 460 34 94
Fragments arranged by size:
460 94 34
Bme1580I G_kGCm'C
Cuts at: 0 107 332 588
Size: 107 225 256
Fragments arranged by size:
256 225 107
BmgBI CAC'GTC
Cuts at: 0 56 588
Size: 56 532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 588
Size: 295 293
BsaAI yAC'GTr
Cuts at: 0 102 588
Size: 102 486
BsaHI Gr'CG_yC
Cuts at: 0 233 588
Size: 233 355
BsiEI CG_ry'CG
Cuts at: 0 201 211 432 588
Size: 201 10 221 156
Fragments arranged by size:
221 201 156 10
BsiHKAI G_wGCw'C
Cuts at: 0 478 588
Size: 478 110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 508 588
Size: 508 80
Bsp1286I G_dGCh'C
Cuts at: 0 107 332 478 588
Size: 107 225 146 110
Fragments arranged by size:
225 146 110 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 368 588
Size: 144 224 220
Fragments arranged by size:
224 220 144
BsrBI CCG'CTC
Cuts at: 0 213 588
Size: 213 375
BssHII G'CGCG_C
Cuts at: 0 525 588
Size: 525 63
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 572 588
Size: 56 516 16
Fragments arranged by size:
516 56 16
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 588
Size: 339 249
BtgI C'CryG_G
Cuts at: 0 437 588
Size: 437 151
DrdI GACnn_nn'nnGTC
Cuts at: 0 232 376 588
Size: 232 144 212
Fragments arranged by size:
232 212 144
EaeI y'GGCC_r
Cuts at: 0 5 128 208 344 429 440 518 588
Size: 5 123 80 136 85 11 78 70
Fragments arranged by size:
136 123 85 80 78 70 11 5
EagI C'GGCC_G
Cuts at: 0 208 429 588
Size: 208 221 159
Fragments arranged by size:
221 208 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 588
Size: 295 293
EcoO109I rG'GnC_Cy
Cuts at: 0 297 588
Size: 297 291
FspI TGC'GCA
Cuts at: 0 364 418 448 588
Size: 364 54 30 140
Fragments arranged by size:
364 140 54 30
FspAI rTGC'GCAy
Cuts at: 0 418 448 588
Size: 418 30 140
Fragments arranged by size:
418 140 30
HaeII r_GCGC'y
Cuts at: 0 169 546 588
Size: 169 377 42
Fragments arranged by size:
377 169 42
MscI TGG'CCA
Cuts at: 0 346 588
Size: 346 242
MslI CAynn'nnrTG
Cuts at: 0 139 241 340 442 588
Size: 139 102 99 102 146
Fragments arranged by size:
146 139 102 102 99
NcoI C'CATG_G
Cuts at: 0 437 588
Size: 437 151
NotI GC'GGCC_GC
Cuts at: 0 208 588
Size: 208 380
NspI r_CATG'y
Cuts at: 0 379 588
Size: 379 209
PpuMI rG'GwC_Cy
Cuts at: 0 297 588
Size: 297 291
PspXI vC'TCGA_Gb
Cuts at: 0 469 588
Size: 469 119
PstI C_TGCA'G
Cuts at: 0 539 588
Size: 539 49
SanDI GG'GwC_CC
Cuts at: 0 297 588
Size: 297 291
SfcI C'TryA_G
Cuts at: 0 535 588
Size: 535 53
SmlI C'TyrA_G
Cuts at: 0 469 588
Size: 469 119
StyI C'CwwG_G
Cuts at: 0 437 488 588
Size: 437 51 100
Fragments arranged by size:
437 100 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 588
Size: 339 249
XhoI C'TCGA_G
Cuts at: 0 469 588
Size: 469 119
XmnI GAAnn'nnTTC
Cuts at: 0 313 588
Size: 313 275
ZraI GAC'GTC
Cuts at: 0 234 588
Size: 234 354
Enzymes that do cut:
AarI AatII AfeI AvaI BbsI BcgI Bme1580I BmgBI
BpmI BsaAI BsaHI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BssHII BstAPI BstXI BtgI DrdI EaeI EagI
Eco57MI EcoO109I FspI FspAI HaeII MscI MslI NcoI
NotI NspI PpuMI PspXI PstI SanDI SfcI SmlI
StyI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BglI BglII BlpI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XmaI