Restriction map for
pF1KB5177
(Linear) (Six-base) MAPSORT of: KIBB5177.seq Check: 3967 from: 1 to: 651
KIBB5177.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 163 651
Size: 163 488
AclI AA'CG_TT
Cuts at: 0 495 651
Size: 495 156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 202 230 249 651
Size: 156 46 28 19 402
Fragments arranged by size:
402 156 46 28 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 651
Size: 64 587
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 243 275 651
Size: 243 32 376
Fragments arranged by size:
376 243 32
ApoI r'AATT_y
Cuts at: 0 33 515 609 651
Size: 33 482 94 42
Fragments arranged by size:
482 94 42 33
AseI AT'TA_AT
Cuts at: 0 245 651
Size: 245 406
AvaI C'yCGr_G
Cuts at: 0 9 651
Size: 9 642
BanI G'GyrC_C
Cuts at: 0 48 651
Size: 48 603
BbvCI CC'TCA_GC
Cuts at: 0 144 651
Size: 144 507
BciVI GTATCCnnnnn_n'
Cuts at: 0 7 103 651
Size: 7 96 548
Fragments arranged by size:
548 96 7
BclI T'GATC_A
Cuts at: 0 393 651
Size: 393 258
BfrBI ATG'CAT
Cuts at: 0 337 651
Size: 337 314
Bme1580I G_kGCm'C
Cuts at: 0 640 651
Size: 640 11
Bpu10I CC'TnA_GC
Cuts at: 0 144 651
Size: 144 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 578 651
Size: 578 73
BsaBI GATnn'nnATC
Cuts at: 0 308 651
Size: 308 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 243 273 651
Size: 243 30 378
Fragments arranged by size:
378 243 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 91 651
Size: 35 56 560
Fragments arranged by size:
560 56 35
BseYI C'CCAG_C
Cuts at: 0 90 651
Size: 90 561
BsmI GAATG_Cn'
Cuts at: 0 41 62 337 651
Size: 41 21 275 314
Fragments arranged by size:
314 275 41 21
Bsp1286I G_dGCh'C
Cuts at: 0 640 651
Size: 640 11
BspHI T'CATG_A
Cuts at: 0 383 423 651
Size: 383 40 228
Fragments arranged by size:
383 228 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 286 651
Size: 286 365
BstZ17I GTA'TAC
Cuts at: 0 164 651
Size: 164 487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 202 230 249 651
Size: 156 46 28 19 402
Fragments arranged by size:
402 156 46 28 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 651
Size: 396 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 378 410 651
Size: 97 32 249 32 241
Fragments arranged by size:
249 241 97 32 32
HindIII A'AGCT_T
Cuts at: 0 550 651
Size: 550 101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 651
Size: 281 370
MslI CAynn'nnrTG
Cuts at: 0 46 651
Size: 46 605
MspA1I CmG'CkG
Cuts at: 0 148 651
Size: 148 503
NsiI A_TGCA'T
Cuts at: 0 339 651
Size: 339 312
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 651
Size: 243 32 376
Fragments arranged by size:
376 243 32
PstI C_TGCA'G
Cuts at: 0 283 651
Size: 283 368
PvuII CAG'CTG
Cuts at: 0 148 651
Size: 148 503
SfcI C'TryA_G
Cuts at: 0 279 651
Size: 279 372
SmaI CCC'GGG
Cuts at: 0 11 651
Size: 11 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 651
Size: 59 32 560
Fragments arranged by size:
560 59 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 447 651
Size: 447 204
XmaI C'CCGG_G
Cuts at: 0 9 651
Size: 9 642
XmnI GAAnn'nnTTC
Cuts at: 0 215 651
Size: 215 436
Enzymes that do cut:
AccI AclI AcuI AlfI AloI ApoI AseI AvaI
BanI BbvCI BciVI BclI BfrBI Bme1580I Bpu10I BsaI
BsaBI BsaXI BseRI BseYI BsmI Bsp1286I BspHI BspMI
BstZ17I Eco57MI FalI Hin4I HindIII MmeI MslI MspA1I
NsiI PpiI PstI PvuII SfcI SmaI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BcgI BglI BglII BlpI
BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaHI
BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI Tth111I XbaI
XhoI ZraI