(Linear) (Six-base) MAPSORT of: KIBB5179.seq  Check: 6406  from: 1  to: 1185



KIBB5179.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0     869    1185 
   Size:        869     316
 
Acc65I G'GTAC_C

Cuts at:      0    1006    1185 
   Size:       1006     179
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    1117    1185 
   Size:        121     996      68

  Fragments arranged by size: 

                 996     121      68
 
ApoI r'AATT_y

Cuts at:      0    1053    1185 
   Size:       1053     132
 
BamHI G'GATC_C

Cuts at:      0     144    1185 
   Size:        144    1041
 
BanI G'GyrC_C

Cuts at:      0     838    1006    1185 
   Size:        838     168     179

  Fragments arranged by size: 

                 838     179     168
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     389    1185 
   Size:        389     796
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     708    1185 
   Size:        708     477
 
BglI GCCn_nnn'nGGC

Cuts at:      0     490     509    1185 
   Size:        490      19     676

  Fragments arranged by size: 

                 676     490      19
 
Bme1580I G_kGCm'C

Cuts at:      0      49    1169    1185 
   Size:         49    1120      16

  Fragments arranged by size: 

                1120      49      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    1185 
   Size:        416     769
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     530    1185 
   Size:        338     192     655

  Fragments arranged by size: 

                 655     338     192
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     392     957    1185 
   Size:        392     565     228

  Fragments arranged by size: 

                 565     392     228
 
BsaBI GATnn'nnATC

Cuts at:      0    1091    1185 
   Size:       1091      94
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     322     352    1185 
   Size:        322      30     833

  Fragments arranged by size: 

                 833     322      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55     454     848    1185 
   Size:         55     399     394     337

  Fragments arranged by size: 

                 399     394     337      55
 
BseYI C'CCAG_C

Cuts at:      0     202    1185 
   Size:        202     983
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577    1185 
   Size:        577     608
 
BsiEI CG_ry'CG

Cuts at:      0      74     873    1185 
   Size:         74     799     312

  Fragments arranged by size: 

                 799     312      74
 
BsiHKAI G_wGCw'C

Cuts at:      0     828    1185 
   Size:        828     357
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     828    1169    1185 
   Size:         49     779     341      16

  Fragments arranged by size: 

                 779     341      49      16
 
BssSI C'ACGA_G

Cuts at:      0      25    1185 
   Size:         25    1160
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     564    1185 
   Size:        564     621
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     465    1185 
   Size:        465     720
 
BstYI r'GATC_y

Cuts at:      0     144    1086    1092    1185 
   Size:        144     942       6      93

  Fragments arranged by size: 

                 942     144      93       6
 
Bsu36I CC'TnA_GG

Cuts at:      0     764    1185 
   Size:        764     421
 
BtgI C'CryG_G

Cuts at:      0    1156    1185 
   Size:       1156      29
 
EaeI y'GGCC_r

Cuts at:      0    1131    1185 
   Size:       1131      54
 
EarI CTCTTCn'nnn_

Cuts at:      0     626    1012    1185 
   Size:        626     386     173

  Fragments arranged by size: 

                 626     386     173
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1054    1185 
   Size:       1054     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     416    1117    1185 
   Size:        121     295     701      68

  Fragments arranged by size: 

                 701     295     121      68
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     139     709    1185 
   Size:        139     570     476

  Fragments arranged by size: 

                 570     476     139
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     779     811    1185 
   Size:        322      32     425      32     374

  Fragments arranged by size: 

                 425     374     322      32      32
 
HincII GTy'rAC

Cuts at:      0     231     870    1185 
   Size:        231     639     315

  Fragments arranged by size: 

                 639     315     231
 
HindIII A'AGCT_T

Cuts at:      0    1174    1185 
   Size:       1174      11
 
KpnI G_GTAC'C

Cuts at:      0    1010    1185 
   Size:       1010     175
 
MfeI C'AATT_G

Cuts at:      0     605    1185 
   Size:        605     580
 
MscI TGG'CCA

Cuts at:      0    1133    1185 
   Size:       1133      52
 
MspA1I CmG'CkG

Cuts at:      0     880    1185 
   Size:        880     305
 
NcoI C'CATG_G

Cuts at:      0    1156    1185 
   Size:       1156      29
 
PasI CC'CwG_GG

Cuts at:      0    1069    1185 
   Size:       1069     116
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     243    1185 
   Size:        243     942
 
PstI C_TGCA'G

Cuts at:      0    1125    1185 
   Size:       1125      60
 
PvuII CAG'CTG

Cuts at:      0     880    1185 
   Size:        880     305
 
SalI G'TCGA_C

Cuts at:      0     868    1185 
   Size:        868     317
 
SfcI C'TryA_G

Cuts at:      0     730    1121    1185 
   Size:        730     391      64

  Fragments arranged by size: 

                 730     391      64
 
SmlI C'TyrA_G

Cuts at:      0     353     545    1185 
   Size:        353     192     640

  Fragments arranged by size: 

                 640     353     192
 
StyI C'CwwG_G

Cuts at:      0     326     520     863    1156    1185 
   Size:        326     194     343     293      29

  Fragments arranged by size: 

                 343     326     293     194      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      60     101     888    1185 
   Size:         60      41     787     297

  Fragments arranged by size: 

                 787     297      60      41
 
XmnI GAAnn'nnTTC

Cuts at:      0    1054    1185 
   Size:       1054     131
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     ApoI    BamHI     BanI     BbsI    BciVI
     BglI Bme1580I     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     EaeI     EarI     EciI  Eco57MI    EcoNI
    Hin4I   HincII  HindIII     KpnI     MfeI     MscI   MspA1I     NcoI
     PasI    PflMI     PstI    PvuII     SalI     SfcI     SmlI     StyI
    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI
   BstEII  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     MluI     MmeI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI