(Linear) (Six-base) MAPSORT of: KIBB5180.seq Check: 3112 from: 1 to: 390
KIBB5180.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 390
Size: 37 353
ApoI r'AATT_y
Cuts at: 0 116 390
Size: 116 274
AvaI C'yCGr_G
Cuts at: 0 88 390
Size: 88 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 390
Size: 291 99
BsaWI w'CCGG_w
Cuts at: 0 153 390
Size: 153 237
BspHI T'CATG_A
Cuts at: 0 35 390
Size: 35 355
BsrGI T'GTAC_A
Cuts at: 0 140 390
Size: 140 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 390
Size: 37 353
MslI CAynn'nnrTG
Cuts at: 0 191 390
Size: 191 199
SmaI CCC'GGG
Cuts at: 0 90 390
Size: 90 300
SmlI C'TyrA_G
Cuts at: 0 306 390
Size: 306 84
TatI w'GTAC_w
Cuts at: 0 140 390
Size: 140 250
XmaI C'CCGG_G
Cuts at: 0 88 390
Size: 88 302
Enzymes that do cut:
AcuI ApoI AvaI BpuEI BsaWI BspHI BsrGI Eco57MI
MslI SmaI SmlI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmnI ZraI