(Linear) (Six-base) MAPSORT of: KIBB5180.seq Check: 3112 from: 1 to: 390 KIBB5180.orf With 182 enzymes: SgfI * April 24, 2009 13:04 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 390 Size: 37 353 ApoI r'AATT_y Cuts at: 0 116 390 Size: 116 274 AvaI C'yCGr_G Cuts at: 0 88 390 Size: 88 302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 291 390 Size: 291 99 BsaWI w'CCGG_w Cuts at: 0 153 390 Size: 153 237 BspHI T'CATG_A Cuts at: 0 35 390 Size: 35 355 BsrGI T'GTAC_A Cuts at: 0 140 390 Size: 140 250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 390 Size: 37 353 MslI CAynn'nnrTG Cuts at: 0 191 390 Size: 191 199 SmaI CCC'GGG Cuts at: 0 90 390 Size: 90 300 SmlI C'TyrA_G Cuts at: 0 306 390 Size: 306 84 TatI w'GTAC_w Cuts at: 0 140 390 Size: 140 250 XmaI C'CCGG_G Cuts at: 0 88 390 Size: 88 302 Enzymes that do cut: AcuI ApoI AvaI BpuEI BsaWI BspHI BsrGI Eco57MI MslI SmaI SmlI TatI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI