Restriction map for
pF1KB5181
(Linear) (Six-base) MAPSORT of: KIBB5181.seq Check: 1198 from: 1 to: 1968
KIBB5181.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 1186 1968
Size: 1186 782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1151 1968
Size: 616 535 817
Fragments arranged by size:
817 616 535
AfeI AGC'GCT
Cuts at: 0 814 1968
Size: 814 1154
AleI CACnn'nnGTG
Cuts at: 0 177 253 916 1968
Size: 177 76 663 1052
Fragments arranged by size:
1052 663 177 76
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1118 1570 1603 1804 1968
Size: 1118 452 33 201 164
Fragments arranged by size:
1118 452 201 164 33
AlwNI CAG_nnn'CTG
Cuts at: 0 775 1237 1353 1442 1836 1968
Size: 775 462 116 89 394 132
Fragments arranged by size:
775 462 394 132 116 89
AvaI C'yCGr_G
Cuts at: 0 430 1640 1776 1968
Size: 430 1210 136 192
Fragments arranged by size:
1210 430 192 136
BanI G'GyrC_C
Cuts at: 0 1042 1186 1968
Size: 1042 144 782
Fragments arranged by size:
1042 782 144
BanII G_rGCy'C
Cuts at: 0 8 74 332 364 404 604 1133 1229
Size: 8 66 258 32 40 200 529 96
Cuts at: 1229 1499 1535 1968
Size: 270 36 433
Fragments arranged by size:
529 433 270 258 200 96 66 40
36 32 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 620 1339 1968
Size: 620 719 629
Fragments arranged by size:
719 629 620
BbvCI CC'TCA_GC
Cuts at: 0 1818 1968
Size: 1818 150
BclI T'GATC_A
Cuts at: 0 920 1968
Size: 920 1048
BfrBI ATG'CAT
Cuts at: 0 269 1968
Size: 269 1699
BglI GCCn_nnn'nGGC
Cuts at: 0 764 1503 1968
Size: 764 739 465
Fragments arranged by size:
764 739 465
BglII A'GATC_T
Cuts at: 0 392 680 1968
Size: 392 288 1288
Fragments arranged by size:
1288 392 288
BlpI GC'TnA_GC
Cuts at: 0 1693 1968
Size: 1693 275
Bme1580I G_kGCm'C
Cuts at: 0 1045 1559 1783 1968
Size: 1045 514 224 185
Fragments arranged by size:
1045 514 224 185
BmgBI CAC'GTC
Cuts at: 0 1668 1968
Size: 1668 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 1094 1250 1968
Size: 87 1007 156 718
Fragments arranged by size:
1007 718 156 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 699 1968
Size: 699 1269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1443 1582 1968
Size: 49 1394 139 386
Fragments arranged by size:
1394 386 139 49
Bpu10I CC'TnA_GC
Cuts at: 0 879 1091 1818 1968
Size: 879 212 727 150
Fragments arranged by size:
879 727 212 150
BsaWI w'CCGG_w
Cuts at: 0 1189 1968
Size: 1189 779
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 448 521 580 721 967 1968
Size: 394 54 73 59 141 246 1001
Fragments arranged by size:
1001 394 246 141 73 59 54
BseYI C'CCAG_C
Cuts at: 0 1053 1230 1275 1367 1453 1576 1706 1968
Size: 1053 177 45 92 86 123 130 262
Fragments arranged by size:
1053 262 177 130 123 92 86 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1968
Size: 673 1295
BsiEI CG_ry'CG
Cuts at: 0 541 1968
Size: 541 1427
BsiHKAI G_wGCw'C
Cuts at: 0 74 332 404 604 997 1133 1535 1699
Size: 74 258 72 200 393 136 402 164
Cuts at: 1699 1968
Size: 269
Fragments arranged by size:
402 393 269 258 200 164 136 74
72
BsmI GAATG_Cn'
Cuts at: 0 702 1968
Size: 702 1266
Bsp1286I G_dGCh'C
Cuts at: 0 8 74 332 364 404 604 997 1045
Size: 8 66 258 32 40 200 393 48
Cuts at: 1045 1133 1229 1499 1535 1559 1699 1783 1968
Size: 88 96 270 36 24 140 84 185
Fragments arranged by size:
393 270 258 200 185 140 96 88
84 66 48 40 36 32 24 8
BspHI T'CATG_A
Cuts at: 0 794 1968
Size: 794 1174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1430 1968
Size: 1430 538
BsrBI CCG'CTC
Cuts at: 0 822 1968
Size: 822 1146
BsrFI r'CCGG_y
Cuts at: 0 976 1968
Size: 976 992
BstAPI GCAn_nnn'nTGC
Cuts at: 0 942 1353 1442 1968
Size: 942 411 89 526
Fragments arranged by size:
942 526 411 89
BstEII G'GTnAC_C
Cuts at: 0 1900 1968
Size: 1900 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 179 258 357 582 1968
Size: 179 79 99 225 1386
Fragments arranged by size:
1386 225 179 99 79
BstYI r'GATC_y
Cuts at: 0 161 392 680 887 1011 1026 1968
Size: 161 231 288 207 124 15 942
Fragments arranged by size:
942 288 231 207 161 124 15
BtgI C'CryG_G
Cuts at: 0 535 1895 1968
Size: 535 1360 73
Fragments arranged by size:
1360 535 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 25 1968
Size: 25 1943
BtsI GCAGTG_nn'
Cuts at: 0 1648 1968
Size: 1648 320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1360 1395 1968
Size: 1360 35 573
Fragments arranged by size:
1360 573 35
DraIII CAC_nnn'GTG
Cuts at: 0 1485 1968
Size: 1485 483
EaeI y'GGCC_r
Cuts at: 0 538 1541 1968
Size: 538 1003 427
Fragments arranged by size:
1003 538 427
EagI C'GGCC_G
Cuts at: 0 538 1968
Size: 538 1430
EarI CTCTTCn'nnn_
Cuts at: 0 268 592 682 844 1633 1968
Size: 268 324 90 162 789 335
Fragments arranged by size:
789 335 324 268 162 90
EcoICRI GAG'CTC
Cuts at: 0 72 330 402 602 1131 1533 1968
Size: 72 258 72 200 529 402 435
Fragments arranged by size:
529 435 402 258 200 72 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 616 1151 1443 1582 1968
Size: 49 567 535 292 139 386
Fragments arranged by size:
567 535 386 292 139 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 64 430 796 913 1968
Size: 64 366 366 117 1055
Fragments arranged by size:
1055 366 366 117 64
EcoO109I rG'GnC_Cy
Cuts at: 0 127 789 1251 1419 1644 1833 1968
Size: 127 662 462 168 225 189 135
Fragments arranged by size:
662 462 225 189 168 135 127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 1905 1968
Size: 333 1572 63
Fragments arranged by size:
1572 333 63
HaeII r_GCGC'y
Cuts at: 0 816 1466 1662 1968
Size: 816 650 196 306
Fragments arranged by size:
816 650 306 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 591 623 1120 1152 1968
Size: 556 32 3 32 497 32 816
Fragments arranged by size:
816 556 497 32 32 32 3
HincII GTy'rAC
Cuts at: 0 960 1968
Size: 960 1008
HindIII A'AGCT_T
Cuts at: 0 499 1968
Size: 499 1469
KpnI G_GTAC'C
Cuts at: 0 1190 1968
Size: 1190 778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 867 1193 1688 1968
Size: 515 352 326 495 280
Fragments arranged by size:
515 495 352 326 280
MscI TGG'CCA
Cuts at: 0 1543 1968
Size: 1543 425
MslI CAynn'nnrTG
Cuts at: 0 177 253 739 916 1552 1968
Size: 177 76 486 177 636 416
Fragments arranged by size:
636 486 416 177 177 76
MspA1I CmG'CkG
Cuts at: 0 413 775 939 1304 1442 1738 1968
Size: 413 362 164 365 138 296 230
Fragments arranged by size:
413 365 362 296 230 164 138
NaeI GCC'GGC
Cuts at: 0 978 1968
Size: 978 990
NcoI C'CATG_G
Cuts at: 0 1895 1968
Size: 1895 73
NgoMIV G'CCGG_C
Cuts at: 0 976 1968
Size: 976 992
NsiI A_TGCA'T
Cuts at: 0 271 1968
Size: 271 1697
NspI r_CATG'y
Cuts at: 0 269 1968
Size: 269 1699
PasI CC'CwG_GG
Cuts at: 0 988 1968
Size: 988 980
PfoI T'CCnGG_A
Cuts at: 0 1415 1968
Size: 1415 553
PpuMI rG'GwC_Cy
Cuts at: 0 789 1419 1644 1833 1968
Size: 789 630 225 189 135
Fragments arranged by size:
789 630 225 189 135
PstI C_TGCA'G
Cuts at: 0 896 1346 1427 1447 1769 1968
Size: 896 450 81 20 322 199
Fragments arranged by size:
896 450 322 199 81 20
PvuII CAG'CTG
Cuts at: 0 413 775 939 1442 1738 1968
Size: 413 362 164 503 296 230
Fragments arranged by size:
503 413 362 296 230 164
SacI G_AGCT'C
Cuts at: 0 74 332 404 604 1133 1535 1968
Size: 74 258 72 200 529 402 433
Fragments arranged by size:
529 433 402 258 200 74 72
SanDI GG'GwC_CC
Cuts at: 0 1644 1968
Size: 1644 324
SapI GCTCTTCn'nnn_
Cuts at: 0 268 592 844 1968
Size: 268 324 252 1124
Fragments arranged by size:
1124 324 268 252
SbfI CC_TGCA'GG
Cuts at: 0 896 1968
Size: 896 1072
SfcI C'TryA_G
Cuts at: 0 408 892 1342 1423 1443 1765 1968
Size: 408 484 450 81 20 322 203
Fragments arranged by size:
484 450 408 322 203 81 20
SmaI CCC'GGG
Cuts at: 0 1642 1968
Size: 1642 326
StuI AGG'CCT
Cuts at: 0 196 454 877 1022 1506 1968
Size: 196 258 423 145 484 462
Fragments arranged by size:
484 462 423 258 196 145
StyI C'CwwG_G
Cuts at: 0 251 1745 1895 1968
Size: 251 1494 150 73
Fragments arranged by size:
1494 251 150 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 928 1843 1888 1968
Size: 928 915 45 80
Fragments arranged by size:
928 915 80 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 213 245 504 536 1471 1503 1968
Size: 213 32 259 32 935 32 465
Fragments arranged by size:
935 465 259 213 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 1952 1968
Size: 147 1805 16
Fragments arranged by size:
1805 147 16
XmaI C'CCGG_G
Cuts at: 0 1640 1968
Size: 1640 328
XmnI GAAnn'nnTTC
Cuts at: 0 1201 1968
Size: 1201 767
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlfI AlwNI AvaI BanI
BanII BbsI BbvCI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI
NspI PasI PfoI PpuMI PstI PvuII SacI SanDI
SapI SbfI SfcI SmaI StuI StyI TaqII TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BmtI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BsiWI BsmBI BspEI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI DraI
DrdI EciI EcoRI EcoRV FseI FspI FspAI HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
ZraI